PDB-7r2o: Crystal structure of a flavodiiron protein S262Y mutant in the ox...

ID or keywords:

Entry

Database: PDB / ID: 7r2o

Title

Crystal structure of a flavodiiron protein S262Y mutant in the oxidized state from Escherichia coli

Components

Anaerobic nitric oxide reductase flavorubredoxin

Keywords

OXIDOREDUCTASE / flavodiiron / nitrosative stress / ROS / FMN / iron

Function / homology

Function and homology information

nitric oxide reductase activity / nitric oxide catabolic process / oxidoreductase activity, acting on other nitrogenous compounds as donors / response to nitric oxide / FMN binding / electron transfer activity / iron ion binding / protein-containing complex / identical protein binding / cytoplasm
Similarity search - Function

Biological species

Escherichia coli K-12 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.85 Å

Authors

Borges, P.T. / Teixeira, M. / Romao, C.V. / Frazao, C.

Funding support

United Kingdom, 1items

Citation

Journal: To Be Published
Title: Crystal structure of a flavodiiron protein S262Y mutant in the oxidized state from Escherichia coli
Authors: Borges, P.T. / Teixeira, M. / Romao, C.V. / Frazao, C.

History

Deposition	Feb 5, 2022	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Feb 15, 2023	Provider: repository / Type: Initial release
Revision 1.1	May 1, 2024	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7r2o.cif.gz	349.4 KB	Display	PDBx/mmCIF format
PDB format	pdb7r2o.ent.gz	282.4 KB	Display	PDB format
PDBx/mmJSON format	7r2o.json.gz		Tree view	PDBx/mmJSON format
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Validation report

Summary document	7r2o_validation.pdf.gz	1.1 MB	Display	wwPDB validaton report
Full document	7r2o_full_validation.pdf.gz	1.1 MB	Display
Data in XML	7r2o_validation.xml.gz	36.1 KB	Display
Data in CIF	7r2o_validation.cif.gz	53 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/r2/7r2o ftp://data.pdbj.org/pub/pdb/validation_reports/r2/7r2o	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#133 - Jan 2011 Nitric Oxide Synthase similarity (3) #187 - Jul 2015 New Delhi Metallo-β-Lactamase similarity (2) #142 - Oct 2011 PDB Pioneers similarity (4) #228 - Dec 2018 Directed Evolution of Enzymes similarity (3) #105 - Sep 2008 Ribonuclease A similarity (2)

Deposited unit

A: Anaerobic nitric oxide reductase flavorubredoxin

B: Anaerobic nitric oxide reductase flavorubredoxin

hetero molecules

110 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Anaerobic nitric oxide reductase flavorubredoxin

B: Anaerobic nitric oxide reductase flavorubredoxin

hetero molecules

A: Anaerobic nitric oxide reductase flavorubredoxin

B: Anaerobic nitric oxide reductase flavorubredoxin

hetero molecules

defined by author
Evidence: gel filtration
220 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

Idetical with deposited unit
defined by author

3

A: Anaerobic nitric oxide reductase flavorubredoxin

hetero molecules

A: Anaerobic nitric oxide reductase flavorubredoxin

hetero molecules

defined by software
110 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

B: Anaerobic nitric oxide reductase flavorubredoxin

hetero molecules

B: Anaerobic nitric oxide reductase flavorubredoxin

hetero molecules

defined by software
110 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	169.968, 64.019, 88.882
Angle α, β, γ (deg.)	90.000, 120.420, 90.000
Int Tables number	5
Space group name H-M	C121

Components on special symmetry positions

ID	Model	Components
1	1	A-608- HOH
2	1	A-853- HOH
3	1	A-871- HOH

#1: Protein	Anaerobic nitric oxide reductase flavorubredoxin / FlRd / FlavoRb Mass: 54369.098 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K12 / Gene: norV, flrD, ygaI, ygaJ, ygaK, b2710, JW2680 / Production host: Escherichia coli (E. coli) / References: UniProt: Q46877
#2: Chemical	ChemComp-FMN / FLAVIN MONONUCLEOTIDE / RIBOFLAVIN MONOPHOSPHATE Mass: 456.344 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₁₇H₂₁N₄O₉P / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical	ChemComp-FEO / MU-OXO-DIIRON Mass: 127.689 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Fe₂O
#4: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₃H₈O₃
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 572 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 1.92 Å³/Da / Density % sol: 35.85 %
Crystal grow	Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.1 M sodium citrate pH 5.6, 20-23% w/v PEG 4000 and 23-25% v/v 2-propanol

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9762 Å
Detector	Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 10, 2017
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9762 Å / Relative weight: 1
Reflection	Resolution: 1.85→58.67 Å / Num. obs: 70227 / % possible obs: 99.4 % / Redundancy: 3.4 % / CC_1/2: 0.992 / Net I/σ(I): 5.68
Reflection shell	Resolution: 1.85→1.96 Å / Num. unique obs: 70227 / CC_1/2: 0.385

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Processing

Software

Name	Version	Classification
XSCALE		data scaling
PHENIX	1.17.1_3660	refinement
PDB_EXTRACT	3.27	data extraction
XDS		data reduction
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: FlRd

Resolution: 1.85→58.67 Å / SU ML: 0.22 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 19.94 / Stereochemistry target values: LS_WUNIT_K1
Details: THE REFINEMENT CONVERGED TO R-WORK AND R-FREE OF 0.187 AND 0.221, RESPECTIVELY. THE FINAL MODEL WAS THEN REFINED VERSUS THE FULL DATA SET.

	R_factor	Num. reflection	% reflection
R_free	0.1837	70199	100 %
R_work	0.1837	70199	-
obs	0.1837	70199	99.66 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso max: 119.47 Å² / B_iso mean: 33.0748 Å² / B_iso min: 16.75 Å²

Refinement step

Cycle: final / Resolution: 1.85→58.67 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	6439	0	97	572	7108
B_iso mean	-	-	39.91	39.21	-
Num. residues	-	-	-	-	800

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0 / Total num. of bins used: 30

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	% reflection obs (%)
1.85-1.87	0.3462	2281	0.3462	2281	4562	99
1.87-1.89	0.3341	2352	0.3341	2352	4704	100
1.89-1.92	0.3283	2288	0.3283	2288	4576	100
1.92-1.94	0.3169	2339	0.3169	2339	4678	100
1.94-1.97	0.3113	2337	0.3113	2337	4674	100
1.97-1.99	0.3061	2309	0.3061	2309	4618	100
1.99-2.02	0.2982	2335	0.2982	2335	4670	100
2.02-2.05	0.2867	2348	0.2867	2348	4696	100
2.05-2.08	0.2769	2308	0.2769	2308	4616	100
2.08-2.12	0.2723	2342	0.2723	2342	4684	100
2.12-2.15	0.2511	2338	0.2511	2338	4676	100
2.15-2.19	0.2444	2345	0.2444	2345	4690	100
2.19-2.24	0.2361	2328	0.2361	2328	4656	100
2.24-2.28	0.233	2323	0.233	2323	4646	100
2.28-2.33	0.2211	2354	0.2211	2354	4708	100
2.33-2.39	0.2265	2318	0.2265	2318	4636	100
2.39-2.44	0.2132	2342	0.2132	2342	4684	100
2.45-2.51	0.2047	2309	0.2047	2309	4618	100
2.51-2.59	0.1984	2348	0.1984	2348	4696	100
2.59-2.67	0.1902	2377	0.1902	2377	4754	100
2.67-2.76	0.187	2305	0.187	2305	4610	100
2.76-2.87	0.1764	2364	0.1764	2364	4728	100
2.87-3.01	0.1719	2332	0.1719	2332	4664	100
3.01-3.16	0.1588	2336	0.1588	2336	4672	99
3.16-3.36	0.1518	2364	0.1518	2364	4728	100
3.36-3.62	0.13	2332	0.13	2332	4664	100
3.62-3.99	0.1223	2364	0.1223	2364	4728	100
3.99-4.56	0.1137	2382	0.1137	2382	4764	100
4.56-5.75	0.1369	2365	0.1369	2365	4730	99
5.75-58.67	0.178	2434	0.178	2434	4868	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.894	0.3936	-2.325	1.4259	0.5423	3.7436	-0.2695	0.2004	-0.449	0.0833	0.196	0.0405	0.5223	-0.3193	0.1953	0.2554	-0.0109	-0.0064	0.2643	-0.0168	0.2746	5.0444	-14.623	14.7862
2	1.6493	-1.4499	-0.4588	2.1387	0.7996	1.964	-0.1367	-0.1803	-0.1389	0.3797	-0.0804	0.0426	-0.0253	-0.0502	0.1936	0.3174	-0.0411	0.0224	0.2384	-0.0198	0.294	-12.1728	-9.3818	28.4651
3	1.4971	0.0081	-0.0391	1.1674	0.1386	1.3849	-0.0985	0.1472	-0.0236	0.0273	0.0429	-0.0288	0.0619	-0.026	0.0659	0.2084	-0.0012	0.0084	0.1938	0.0184	0.1994	8.9489	-8.717	18.6633
4	8.1923	-0.7684	-0.2656	4.6672	-0.7291	2.6782	-0.1252	-0.7265	-0.8683	0.2773	0.1769	0.1373	0.4288	-0.0008	-0.0566	0.345	0.004	-0.0065	0.251	0.0564	0.3532	8.9388	-15.5898	27.8388
5	0.9213	-0.3814	0.0948	2.0383	-0.1166	1.4249	-0.0727	-0.0924	0.0105	0.2174	0.121	-0.242	0.1452	0.068	-0.0602	0.227	-0.0015	-0.0237	0.2267	0.0104	0.2116	11.3803	-3.04	26.3185
6	1.3868	0.2304	-0.0705	0.8623	0.1722	1.5893	-0.0522	-0.1771	0.1633	0.0692	-0.0002	-0.0027	-0.1804	0.23	0.0423	0.2076	-0.0094	-0.0155	0.2266	-0.0124	0.2279	11.9086	4.6765	28.1629
7	3.6224	-1.9047	-1.2646	3.8938	1.9075	5.1845	0.2167	0.5453	0.2176	-0.5281	0.0795	-0.9459	-0.3681	0.1099	-0.1895	0.2556	-0.0196	0.0408	0.3052	0.0057	0.3527	16.5508	-2.1682	8.7297
8	1.1825	-0.0808	-0.2851	0.607	0.0922	1.1834	-0.0562	-0.0331	-0.1321	-0.0297	-0.0296	0.0466	0.1026	-0.0973	0.0968	0.2094	-0.0116	-0.0041	0.2195	0.0015	0.1929	-3.2528	-3.8929	15.1617
9	1.6882	0.3994	-0.8815	1.0931	0.0153	0.8452	-0.043	0.129	0.1446	0.086	-0.026	0.0601	-0.0478	-0.1031	0.0401	0.2124	0.0018	-0.0229	0.2605	0.0208	0.2214	-7.6833	6.7987	14.3681
10	1.0855	0.3175	-0.051	0.7148	0.0359	0.838	0.0409	0.125	-0.0747	-0.0135	0.0263	0.1452	0.1975	0.0285	0.0098	0.233	-0.0141	0.0006	0.2187	-0.0238	0.2018	-3.9026	-5.5513	8.8272
11	6.0869	-1.826	-1.2692	2.0142	-0.0152	0.6563	0.1801	0.6957	-0.0601	0.0016	-0.1712	-0.1033	-0.0129	-0.1049	0.0538	0.247	-0.0183	0.0067	0.2618	0.0111	0.1876	-16.6723	5.7297	11.14
12	3.145	-0.366	-0.7484	1.0926	-0.3551	1.6215	-0.1318	0.3545	-0.2563	-0.0809	0.0241	0.1018	0.128	-0.1872	0.0904	0.2275	-0.044	0.0098	0.2424	-0.0424	0.2433	-34.8821	1.0439	25.0772
13	1.4587	0.062	0.8221	0.437	-0.1761	1.559	-0.033	-0.0125	-0.0307	0.0463	-0.0159	0.0037	-0.0361	-0.0677	0.0233	0.205	-0.0043	-0.0145	0.1903	0.0107	0.1892	-28.1398	6.5737	31.1104
14	4.3706	-0.7092	0.0154	1.4493	0.2903	3.7509	0.1683	0.2759	0.3265	-0.1123	0.0419	-0.1566	-0.0798	0.1579	-0.0737	0.2577	-0.0284	0.0033	0.2271	0.03	0.2708	-22.1143	11.9487	23.4292
15	2.5641	0.7035	-1.2729	1.1451	-0.9922	1.9755	0.2219	0.0438	0.2824	0.2359	-0.0831	0.1576	-0.4655	-0.0475	-0.0792	0.2481	-0.0117	0.0133	0.2455	0.0054	0.2828	-37.4544	12.3927	37.566
16	4.3346	0.4829	-0.558	2.3574	0.8023	2.1268	0.1132	-0.0823	0.5047	-0.059	0.0105	-0.0486	-0.1366	-0.2002	-0.0263	0.2784	-0.0072	-0.0104	0.178	0.0325	0.3013	-28.9166	16.6494	27.6884
17	2.0283	0.1083	-0.8003	1.0706	-1.1999	3.2582	-0.0127	0.6921	0.1783	-0.0421	0.0356	0.1923	-0.1533	-0.6398	-0.0427	0.2581	0.0206	-0.0192	0.4071	0.0314	0.2758	-44.0745	9.4966	23.1329
18	2.7004	-0.0544	1.7799	1.5011	-0.4559	3.5644	-0.1741	0.4685	0.5826	-0.0334	0.1595	0.1793	-0.4499	0.3102	0.0399	0.2862	-0.0562	-0.0396	0.329	0.0689	0.3165	-50.0027	-9.2333	14.5475
19	1.446	-1.0618	0.4126	2.3869	-0.388	1.7379	-0.0378	-0.0888	0.2181	0.4128	-0.2192	-0.0684	0.0555	0.0255	0.2909	0.3372	-0.0487	-0.0446	0.2508	0.0212	0.3365	-32.7997	-14.2803	28.3782
20	1.4148	-0.2768	-0.0294	1.1019	-0.095	1.898	-0.0793	0.1602	0.1057	-0.085	0.0692	0.0658	-0.1349	-0.0209	0.015	0.2499	-0.0252	-0.0607	0.2566	0.0134	0.2854	-53.9057	-15.0515	18.5018
21	2.5309	-0.3447	-0.6793	2.7147	0.4878	1.2257	-0.0064	-0.0925	0.3566	0.0547	0.098	0.0279	-0.3015	0.0659	-0.1227	0.2614	-0.0008	-0.0097	0.2263	-0.0194	0.3028	-54.2978	-13.2979	25.8574
22	0.9933	-0.057	-0.1867	1.1609	-0.3383	1.7964	0.0363	-0.1441	0.0315	0.1003	0.0647	0.0627	-0.1889	-0.1301	-0.0935	0.2096	-0.019	-0.0042	0.2353	-0.0245	0.2336	-57.1342	-23.9909	29.6297
23	4.366	-2.1857	-0.9204	4.9497	1.4945	1.7011	0.0059	0.5817	-0.3923	-0.1955	-0.3058	0.8584	0.2154	-0.4135	0.0997	0.2622	-0.0663	-0.0002	0.4193	-0.0909	0.3378	-58.7151	-30.7516	13.0818
24	0.9635	-0.1356	-0.3325	1.4779	0.0022	0.9145	-0.023	0.3024	-0.0178	-0.1342	-0.0479	0.0889	0.062	0.0329	0.0393	0.201	-0.0262	-0.0317	0.2636	-0.0251	0.2154	-50.9096	-25.8482	13.9943
25	1.4186	0.1964	0.286	1.728	-0.2361	1.8208	-0.0767	0.3021	0.3619	-0.1492	0.0744	-0.1013	-0.1897	0.3089	0.034	0.2348	-0.0769	-0.0147	0.3382	0.0497	0.2717	-35.0765	-13.1496	14.5667
26	1.5967	0.4302	0.6429	1.2429	0.1336	0.7702	-0.0378	0.0012	-0.152	-0.1131	-0.1082	-0.0604	0.0385	-0.1533	0.1892	0.2288	-0.0185	0.011	0.3035	-0.0747	0.2667	-36.4518	-30.8782	15.0998
27	1.9088	0.1817	-0.1746	0.6992	-0.1651	1.2913	0.0341	0.3574	0.095	-0.1635	0.0362	-0.0238	0.1547	0.1235	0.0236	0.27	-0.0254	-0.0287	0.3646	0.0241	0.2358	-42.2119	-18.7474	7.9161
28	3.1455	-0.6202	0.6618	0.9078	-0.2764	0.4111	-0.039	0.4948	-0.0146	0.0169	0.0084	0.2322	0.0301	0.24	0.0119	0.2596	-0.0337	-0.0142	0.392	-0.0268	0.23	-27.1499	-28.4614	11.1006
29	2.3202	0.0664	1.1363	1.21	0.26	1.7045	-0.1222	0.1783	0.1434	-0.012	0.0438	-0.0618	-0.1251	0.2572	0.0956	0.1987	-0.031	-0.0092	0.2341	0.0313	0.2306	-9.7255	-24.4773	25.9065
30	1.744	0.1586	-0.785	0.4636	-0.0551	1.3517	-0.13	0.114	-0.1269	0.0562	0.0636	0.0009	0.0044	0.0493	0.0227	0.2184	-0.0141	-0.001	0.1965	-0.0056	0.2396	-18.1919	-30.5494	30.7203
31	3.216	-0.5794	2.5258	1.1517	0.458	3.111	0.201	0.004	-0.3902	0.0462	0.0017	0.1259	0.1761	-0.0144	-0.3153	0.1982	-0.04	0.0306	0.1983	-0.0047	0.2853	-13.255	-35.829	29.0802
32	2.1739	0.7059	1.4908	1.6525	-0.6932	2.5017	0.1139	-0.0478	-0.2875	-0.1375	-0.0019	-0.2037	0.25	0.0028	-0.0551	0.2404	-0.0108	0.02	0.1714	-0.0211	0.3023	-16.1785	-39.5311	31.1451
33	1.5109	0.0764	0.7284	0.7207	0.8532	2.1873	0.0228	0.0914	-0.0783	0.0748	-0.0172	0.0025	0.0346	0.4542	-0.0165	0.2362	0.0006	0.0004	0.2538	0.0121	0.2875	0.2511	-32.7249	31.6731
34	1.1381	-0.4758	0.8122	1.7034	0.6583	4.6301	0.1282	0.9202	-0.1061	-0.0417	-0.1418	-0.2942	0.4302	0.7605	-0.0478	0.2801	0.0098	0.0465	0.4724	-0.0474	0.3406	-3.2809	-34.5365	16.7694

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	(chain A and resid 2:15)	A	2 - 15
2	X-RAY DIFFRACTION	2	(chain A and resid 16:33)	A	16 - 33
3	X-RAY DIFFRACTION	3	(chain A and resid 34:52)	A	34 - 52
4	X-RAY DIFFRACTION	4	(chain A and resid 53:69)	A	53 - 69
5	X-RAY DIFFRACTION	5	(chain A and resid 70:106)	A	70 - 106
6	X-RAY DIFFRACTION	6	(chain A and resid 107:129)	A	107 - 129
7	X-RAY DIFFRACTION	7	(chain A and resid 130:138)	A	130 - 138
8	X-RAY DIFFRACTION	8	(chain A and resid 139:190)	A	139 - 190
9	X-RAY DIFFRACTION	9	(chain A and resid 191:218)	A	191 - 218
10	X-RAY DIFFRACTION	10	(chain A and resid 219:239)	A	219 - 239
11	X-RAY DIFFRACTION	11	(chain A and resid 240:251)	A	240 - 251
12	X-RAY DIFFRACTION	12	(chain A and resid 252:298)	A	252 - 298
13	X-RAY DIFFRACTION	13	(chain A and resid 299:328)	A	299 - 328
14	X-RAY DIFFRACTION	14	(chain A and resid 329:341)	A	329 - 341
15	X-RAY DIFFRACTION	15	(chain A and resid 342:356)	A	342 - 356
16	X-RAY DIFFRACTION	16	(chain A and resid 357:370)	A	357 - 370
17	X-RAY DIFFRACTION	17	(chain A and resid 371:402)	A	371 - 402
18	X-RAY DIFFRACTION	18	(chain B and resid 2:15)	B	2 - 15
19	X-RAY DIFFRACTION	19	(chain B and resid 16:33)	B	16 - 33
20	X-RAY DIFFRACTION	20	(chain B and resid 34:52)	B	34 - 52
21	X-RAY DIFFRACTION	21	(chain B and resid 53:83)	B	53 - 83
22	X-RAY DIFFRACTION	22	(chain B and resid 84:124)	B	84 - 124
23	X-RAY DIFFRACTION	23	(chain B and resid 125:131)	B	125 - 131
24	X-RAY DIFFRACTION	24	(chain B and resid 132:164)	B	132 - 164
25	X-RAY DIFFRACTION	25	(chain B and resid 165:191)	B	165 - 191
26	X-RAY DIFFRACTION	26	(chain B and resid 192:216)	B	192 - 216
27	X-RAY DIFFRACTION	27	(chain B and resid 217:237)	B	217 - 237
28	X-RAY DIFFRACTION	28	(chain B and resid 238:253)	B	238 - 253
29	X-RAY DIFFRACTION	29	(chain B and resid 254:298)	B	254 - 298
30	X-RAY DIFFRACTION	30	(chain B and resid 299:333)	B	299 - 333
31	X-RAY DIFFRACTION	31	(chain B and resid 334:350)	B	334 - 350
32	X-RAY DIFFRACTION	32	(chain B and resid 351:366)	B	351 - 366
33	X-RAY DIFFRACTION	33	(chain B and resid 367:382)	B	367 - 382
34	X-RAY DIFFRACTION	34	(chain B and resid 383:402)	B	383 - 402

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