[English] 日本語

Yorodumi

- PDB-7qnm: Crystallization and structural analyses of ZgHAD, a L-2-haloacid ... -

+
Open data

-
Basic information

Entry

Database: PDB / ID: 7qnm

Title

Crystallization and structural analyses of ZgHAD, a L-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans

Components

(S)-2-haloacid dehalogenase

Keywords

HYDROLASE / haloacid dehalogenases / marine / moint-mutant / oligomerisation

Function / homology

Function and homology information

(S)-2-haloacid dehalogenase / (S)-2-haloacid dehalogenase activity
Similarity search - Function

Biological species

Zobellia galactanivorans (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.73 Å

Authors

Grigorian, E. / Roret, T. / Leblanc, C. / Delage, L. / Czjzek, M.

Funding support

1items

Citation

Journal: Protein Sci. / Year: 2023
Title: X-ray structure and mechanism of ZgHAD, a l-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans.
Authors: Grigorian, E. / Roret, T. / Czjzek, M. / Leblanc, C. / Delage, L.

History

Deposition	Dec 21, 2021	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Dec 21, 2022	Provider: repository / Type: Initial release
Revision 1.1	Jan 11, 2023	Group: Database references / Category: citation Item: _citation.journal_volume / _citation.title / _citation.year
Revision 1.2	Feb 7, 2024	Group: Data collection / Category: chem_comp_atom / chem_comp_bond
Revision 1.3	Feb 14, 2024	Group: Refinement description / Category: pdbx_initial_refinement_model / Item: _pdbx_initial_refinement_model.accession_code

-
Structure visualization

Structure viewer	Molecule: MolmilJmol/JSmol

-
Downloads & links

-
Download

PDBx/mmCIF format	7qnm.cif.gz	1.1 MB	Display	PDBx/mmCIF format
PDB format	pdb7qnm.ent.gz	902.5 KB	Display	PDB format
PDBx/mmJSON format	7qnm.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

-
Validation report

Summary document	7qnm_validation.pdf.gz	660.5 KB	Display	wwPDB validaton report
Full document	7qnm_full_validation.pdf.gz	749.4 KB	Display
Data in XML	7qnm_validation.xml.gz	189.2 KB	Display
Data in CIF	7qnm_validation.cif.gz	254.7 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/qn/7qnm ftp://data.pdbj.org/pub/pdb/validation_reports/qn/7qnm	HTTPS FTP

-
Related structure data

Related structure data	7arpC 7aszSC C: citing same article (ref.) S: Starting model for refinement
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

-
Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#220 - Apr 2018 Dehalogenases similarity (5) #118 - Oct 2009 Sodium-Potassium Pump similarity (1)

-
Assembly

Deposited unit

A: (S)-2-haloacid dehalogenase

B: (S)-2-haloacid dehalogenase

C: (S)-2-haloacid dehalogenase

D: (S)-2-haloacid dehalogenase

E: (S)-2-haloacid dehalogenase

F: (S)-2-haloacid dehalogenase

G: (S)-2-haloacid dehalogenase

H: (S)-2-haloacid dehalogenase

I: (S)-2-haloacid dehalogenase

J: (S)-2-haloacid dehalogenase

K: (S)-2-haloacid dehalogenase

L: (S)-2-haloacid dehalogenase

M: (S)-2-haloacid dehalogenase

N: (S)-2-haloacid dehalogenase

O: (S)-2-haloacid dehalogenase

P: (S)-2-haloacid dehalogenase

Q: (S)-2-haloacid dehalogenase

R: (S)-2-haloacid dehalogenase

S: (S)-2-haloacid dehalogenase

T: (S)-2-haloacid dehalogenase

U: (S)-2-haloacid dehalogenase

V: (S)-2-haloacid dehalogenase

W: (S)-2-haloacid dehalogenase

X: (S)-2-haloacid dehalogenase

Y: (S)-2-haloacid dehalogenase

Z: (S)-2-haloacid dehalogenase

hetero molecules

656 kDa, 26 polymers
Search similar-shape structures of this assembly by Omokage search (details)

A: (S)-2-haloacid dehalogenase

B: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

C: (S)-2-haloacid dehalogenase

D: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

E: (S)-2-haloacid dehalogenase

F: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

G: (S)-2-haloacid dehalogenase

H: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

I: (S)-2-haloacid dehalogenase

J: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

K: (S)-2-haloacid dehalogenase

L: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

M: (S)-2-haloacid dehalogenase

N: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

O: (S)-2-haloacid dehalogenase

P: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Q: (S)-2-haloacid dehalogenase

R: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

S: (S)-2-haloacid dehalogenase

T: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

U: (S)-2-haloacid dehalogenase

V: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

W: (S)-2-haloacid dehalogenase

X: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Y: (S)-2-haloacid dehalogenase

Z: (S)-2-haloacid dehalogenase

hetero molecules

defined by author&software
Evidence: gel filtration
50.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	76.165, 132.789, 275.698
Angle α, β, γ (deg.)	90.00, 92.31, 90.00
Int Tables number	4
Space group name H-M	P12₁1
Symmetry operation	#1: x,y,z #2: -x,y+1/2,-z

Noncrystallographic symmetry (NCS)

NCS domain:
NCS ensembles :

-
Components

#1: Protein	... (S)-2-haloacid dehalogenase Mass: 25148.123 Da / Num. of mol.: 26 / Mutation: H179N Source method: isolated from a genetically manipulated source Source: (gene. exp.) Zobellia galactanivorans (bacteria) Strain: DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij Gene: dhlB, zobellia_4183 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: G0L7V6, (S)-2-haloacid dehalogenase
#2: Chemical	... ChemComp-PO4 / PHOSPHATE ION Mass: 94.971 Da / Num. of mol.: 26 / Source method: obtained synthetically / Formula: PO₄
#3: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 55 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	N

-
Experimental details

-
Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

Crystal	Density Matthews: 2.13 Å³/Da / Density % sol: 42.27 %
Crystal grow	Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 25% (w/v) PEG3350, 0.1 M Tris-HCl pH 8.5 and 0.2 M NaCl

-
Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.980114 Å
Detector	Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Oct 15, 2019
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.980114 Å / Relative weight: 1
Reflection	Resolution: 2.73→49.51 Å / Num. obs: 144327 / % possible obs: 99.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.9 % / B_iso Wilson estimate: 65.43 Å² / CC_1/2: 0.998 / R_pim(I) all: 0.054 / R_rim(I) all: 0.143 / Χ²: 0.92 / Net I/av σ(I): 9.6 / Net I/σ(I): 5
Reflection shell	Resolution: 2.75→2.8 Å / Redundancy: 6.2 % / Mean I/σ(I) obs: 1 / Num. unique obs: 36555 / CC_1/2: 0.517 / R_pim(I) all: 0.78 / R_rim(I) all: 1.475 / Χ²: 0.79 / % possible all: 83.7

-
Processing

Software

Name	Version	Classification
PHENIX	1.18.2_3874	refinement
REFMAC	5.8.0267	refinement
XDS		data reduction
XSCALE		data scaling
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 7ASZ

7asz

Resolution: 2.73→49.51 Å / Cor.coef. F_o:F_c: 0.95 / Cor.coef. F_o:F_c free: 0.921 / SU B: 19.622 / SU ML: 0.37 / Cross valid method: THROUGHOUT / ESU R Free: 0.407 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.25434	7202	5 %	RANDOM
R_work	0.20646	-	-	-
obs	0.20881	136695	98.79 %	-

Solvent computation

Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK

Displacement parameters

B_iso mean: 83.714 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	-4.31 Å²	0 Å²	0.44 Å²
2-	-	-0.18 Å²	0 Å²
3-	-	-	4.44 Å²

Refinement step

Cycle: 1 / Resolution: 2.73→49.51 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	44819	0	130	55	45004

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.009	0.013	45677
X-RAY DIFFRACTION	r_bond_other_d	0.005	0.017	45201
X-RAY DIFFRACTION	r_angle_refined_deg	1.502	1.637	61715
X-RAY DIFFRACTION	r_angle_other_deg	1.225	1.579	104055
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	6.055	5	5731
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	29.323	23.049	2132
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	14.766	15	8390
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	15.025	15	234
X-RAY DIFFRACTION	r_chiral_restr	0.052	0.2	5965
X-RAY DIFFRACTION	r_gen_planes_refined	0.007	0.02	50751
X-RAY DIFFRACTION	r_gen_planes_other	0.013	0.02	9813
X-RAY DIFFRACTION	r_nbd_refined
X-RAY DIFFRACTION	r_nbd_other
X-RAY DIFFRACTION	r_nbtor_refined
X-RAY DIFFRACTION	r_nbtor_other
X-RAY DIFFRACTION	r_xyhbond_nbd_refined
X-RAY DIFFRACTION	r_xyhbond_nbd_other
X-RAY DIFFRACTION	r_metal_ion_refined
X-RAY DIFFRACTION	r_metal_ion_other
X-RAY DIFFRACTION	r_symmetry_vdw_refined
X-RAY DIFFRACTION	r_symmetry_vdw_other
X-RAY DIFFRACTION	r_symmetry_hbond_refined
X-RAY DIFFRACTION	r_symmetry_hbond_other
X-RAY DIFFRACTION	r_symmetry_metal_ion_refined
X-RAY DIFFRACTION	r_symmetry_metal_ion_other
X-RAY DIFFRACTION	r_mcbond_it	5.59	8.569	23002
X-RAY DIFFRACTION	r_mcbond_other	5.589	8.569	23001
X-RAY DIFFRACTION	r_mcangle_it	8.289	12.853	28707
X-RAY DIFFRACTION	r_mcangle_other	8.289	12.853	28708
X-RAY DIFFRACTION	r_scbond_it	5.855	9.164	22675
X-RAY DIFFRACTION	r_scbond_other	5.854	9.164	22672
X-RAY DIFFRACTION	r_scangle_it
X-RAY DIFFRACTION	r_scangle_other	9.17	13.496	33003
X-RAY DIFFRACTION	r_long_range_B_refined	11.979		48805
X-RAY DIFFRACTION	r_long_range_B_other	11.979		48805
X-RAY DIFFRACTION	r_rigid_bond_restr
X-RAY DIFFRACTION	r_sphericity_free
X-RAY DIFFRACTION	r_sphericity_bonded

Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION

Ens-ID	Dom-ID	Auth asym-ID	Number	Type	Rms dev position (Å)	Weight position
10	10	A	1712	tight positional	0.23	0.25
11	11	B	1720	tight positional	0.21	0.25
12	12	C	1720	tight positional	0.25	0.25
13	13	D	1712	tight positional	0.26	0.25
14	14	E	1720	tight positional	0.22	0.25
15	15	F	1712	tight positional	0.23	0.25
16	16	G	1712	tight positional	0.23	0.25
17	17	H	1712	tight positional	0.25	0.25
18	18	I	1720	tight positional	0.23	0.25
19	19	J	1712	tight positional	0.22	0.25
20	20	K	1720	tight positional	0.21	0.25
21	21	L	1720	tight positional	0.21	0.25
22	22	M	1720	tight positional	0.29	0.25
23	23	N	1720	tight positional	0.2	0.25
24	24	O	1720	tight positional	0.22	0.25
25	25	P	1720	tight positional	0.23	0.25
26	26	Q	1712	tight positional	0.22	0.25
27	27	R	1720	tight positional	0.24	0.25
28	28	S	1720	tight positional	0.22	0.25
29	29	T	1720	tight positional	0.25	0.25
30	30	U	1712	tight positional	0.25	0.25
31	31	V	1712	tight positional	0.25	0.25
32	32	W	1712	tight positional	0.23	0.25
33	33	X	1712	tight positional	0.2	0.25
34	34	Y	1712	tight positional	0.23	0.25
35	35	Z	1720	tight positional	0.24	0.25
36	36	A	1720	tight positional	0.27	0.25
37	37	B	1712	tight positional	0.25	0.25
38	38	C	1720	tight positional	0.26	0.25
39	39	D	1712	tight positional	0.23	0.25
40	40	E	1712	tight positional	0.26	0.25
41	41	F	1712	tight positional	0.25	0.25
42	42	G	1720	tight positional	0.24	0.25
43	43	H	1712	tight positional	0.22	0.25
44	44	I	1720	tight positional	0.19	0.25
45	45	J	1720	tight positional	0.23	0.25
46	46	K	1720	tight positional	0.29	0.25
47	47	L	1720	tight positional	0.23	0.25
48	48	M	1720	tight positional	0.25	0.25
49	49	N	1720	tight positional	0.22	0.25
50	50	O	1712	tight positional	0.21	0.25
51	51	P	1712	tight positional	0.2	0.25
52	52	Q	1712	tight positional	0.23	0.25
53	53	R	1729	tight positional	0.24	0.25
54	54	S	1729	tight positional	0.27	0.25
55	55	T	1729	tight positional	0.23	0.25
56	56	U	1729	tight positional	0.22	0.25
57	57	V	1729	tight positional	0.21	0.25
58	58	W	1712	tight positional	0.19	0.25
59	59	X	1712	tight positional	0.24	0.25
60	60	Y	1729	tight positional	0.26	0.25
61	61	Z	1712	tight positional	0.2	0.25
62	62	A	1729	tight positional	0.27	0.25
63	63	B	1729	tight positional	0.25	0.25
64	64	C	1729	tight positional	0.26	0.25
65	65	D	1712	tight positional	0.24	0.25
66	66	E	1729	tight positional	0.22	0.25
67	67	F	1712	tight positional	0.22	0.25
68	68	G	1712	tight positional	0.2	0.25
69	69	H	1712	tight positional	0.27	0.25
70	70	I	1712	tight positional	0.23	0.25
71	71	J	1712	tight positional	0.2	0.25
72	72	K	1712	tight positional	0.2	0.25
73	73	L	1720	tight positional	0.23	0.25
74	74	M	1720	tight positional	0.26	0.25
75	75	N	1712	tight positional	0.25	0.25
76	76	O	1712	tight positional	0.24	0.25
77	77	P	1712	tight positional	0.22	0.25
78	78	Q	1712	tight positional	0.23	0.25
79	79	R	1712	tight positional	0.22	0.25
80	80	S	1720	tight positional	0.21	0.25
81	81	T	1720	tight positional	0.27	0.25
82	82	U	1712	tight positional	0.23	0.25
83	83	V	1720	tight positional	0.22	0.25
84	84	W	1712	tight positional	0.27	0.25
85	85	X	1712	tight positional	0.25	0.25
86	86	Y	1712	tight positional	0.22	0.25
87	87	Z	1720	tight positional	0.24	0.25
88	88	A	1712	tight positional	0.22	0.25
89	89	B	1720	tight positional	0.24	0.25
90	90	C	1720	tight positional	0.19	0.25
91	91	D	1720	tight positional	0.29	0.25
92	92	E	1720	tight positional	0.22	0.25
93	93	F	1720	tight positional	0.23	0.25
94	94	G	1720	tight positional	0.24	0.25
95	95	H	1720	tight positional	0.24	0.25
96	96	I	1712	tight positional	0.22	0.25
97	97	J	1712	tight positional	0.21	0.25
98	98	K	1712	tight positional	0.25	0.25
99	99	L	1712	tight positional	0.2	0.25
100	100	M	1712	tight positional	0.21	0.25
101	101	N	1720	tight positional	0.22	0.25
102	102	O	1720	tight positional	0.25	0.25
103	103	P	1712	tight positional	0.25	0.25
104	104	Q	1720	tight positional	0.22	0.25
105	105	R	1712	tight positional	0.25	0.25
106	106	S	1712	tight positional	0.23	0.25
107	107	T	1712	tight positional	0.23	0.25
108	108	U	1720	tight positional	0.23	0.25
109	109	V	1712	tight positional	0.21	0.25
110	110	W	1720	tight positional	0.21	0.25
111	111	X	1720	tight positional	0.22	0.25
112	112	Y	1720	tight positional	0.29	0.25
113	113	Z	1720	tight positional	0.23	0.25
114	114	A	1720	tight positional	0.21	0.25
115	115	B	1720	tight positional	0.21	0.25
116	116	C	1712	tight positional	0.23	0.25
117	117	D	1712	tight positional	0.26	0.25
118	118	E	1712	tight positional	0.25	0.25
119	119	F	1712	tight positional	0.22	0.25
120	120	G	1712	tight positional	0.22	0.25
121	121	H	1720	tight positional	0.23	0.25
122	122	I	1720	tight positional	0.28	0.25
123	123	J	1712	tight positional	0.25	0.25
124	124	K	1720	tight positional	0.22	0.25
125	125	L	1712	tight positional	0.25	0.25
126	126	M	1712	tight positional	0.23	0.25
127	127	N	1712	tight positional	0.26	0.25
128	128	O	1720	tight positional	0.25	0.25
129	129	P	1712	tight positional	0.23	0.25
130	130	Q	1720	tight positional	0.24	0.25
131	131	R	1720	tight positional	0.23	0.25
132	132	S	1720	tight positional	0.29	0.25
133	133	T	1720	tight positional	0.23	0.25
134	134	U	1720	tight positional	0.22	0.25
135	135	V	1720	tight positional	0.24	0.25
136	136	W	1729	tight positional	0.28	0.25
137	137	X	1729	tight positional	0.26	0.25
138	138	Y	1729	tight positional	0.24	0.25
139	139	Z	1729	tight positional	0.25	0.25
140	140	A	1712	tight positional	0.22	0.25
141	141	B	1712	tight positional	0.24	0.25
142	142	C	1729	tight positional	0.24	0.25
143	143	D	1712	tight positional	0.19	0.25
144	144	E	1729	tight positional	0.25	0.25
145	145	F	1729	tight positional	0.26	0.25
146	146	G	1729	tight positional	0.28	0.25
147	147	H	1712	tight positional	0.23	0.25
148	148	I	1729	tight positional	0.26	0.25
149	149	J	1712	tight positional	0.21	0.25
150	150	K	1712	tight positional	0.22	0.25
151	151	L	1712	tight positional	0.28	0.25
152	152	M	1712	tight positional	0.23	0.25
153	153	N	1712	tight positional	0.23	0.25
154	154	O	1712	tight positional	0.23	0.25
155	155	P	1729	tight positional	0.29	0.25
156	156	Q	1729	tight positional	0.3	0.25
157	157	R	1729	tight positional	0.27	0.25
158	158	S	1712	tight positional	0.24	0.25
159	159	T	1712	tight positional	0.22	0.25
160	160	U	1729	tight positional	0.3	0.25
161	161	V	1712	tight positional	0.22	0.25
162	162	W	1729	tight positional	0.33	0.25
163	163	X	1729	tight positional	0.29	0.25
164	164	Y	1729	tight positional	0.24	0.25
165	165	Z	1712	tight positional	0.23	0.25
166	166	A	1729	tight positional	0.23	0.25
167	167	B	1712	tight positional	0.23	0.25
168	168	C	1712	tight positional	0.25	0.25
169	169	D	1712	tight positional	0.23	0.25
170	170	E	1712	tight positional	0.22	0.25
171	171	F	1712	tight positional	0.22	0.25
172	172	G	1712	tight positional	0.23	0.25
173	173	H	1729	tight positional	0.23	0.25
174	174	I	1729	tight positional	0.23	0.25
175	175	J	1712	tight positional	0.2	0.25
176	176	K	1712	tight positional	0.26	0.25
177	177	L	1729	tight positional	0.24	0.25
178	178	M	1712	tight positional	0.23	0.25
179	179	N	1729	tight positional	0.28	0.25
180	180	O	1729	tight positional	0.26	0.25
181	181	P	1729	tight positional	0.26	0.25
182	182	Q	1712	tight positional	0.23	0.25
183	183	R	1729	tight positional	0.25	0.25
184	184	S	1712	tight positional	0.22	0.25
185	185	T	1712	tight positional	0.22	0.25
186	186	U	1712	tight positional	0.29	0.25
187	187	V	1712	tight positional	0.23	0.25
188	188	W	1712	tight positional	0.23	0.25
189	189	X	1712	tight positional	0.24	0.25
190	190	Y	1729	tight positional	0.22	0.25
191	191	Z	1712	tight positional	0.21	0.25
192	192	A	1712	tight positional	0.24	0.25
193	193	B	1729	tight positional	0.25	0.25
194	194	C	1712	tight positional	0.23	0.25
195	195	D	1729	tight positional	0.24	0.25
196	196	E	1729	tight positional	0.25	0.25
197	197	F	1729	tight positional	0.29	0.25
198	198	G	1712	tight positional	0.24	0.25
199	199	H	1729	tight positional	0.22	0.25
200	200	I	1712	tight positional	0.2	0.25
201	201	J	1712	tight positional	0.21	0.25
202	202	K	1712	tight positional	0.29	0.25
203	203	L	1712	tight positional	0.21	0.25
204	204	M	1712	tight positional	0.22	0.25
205	205	N	1712	tight positional	0.22	0.25
206	206	O	1712	tight positional	0.2	0.25
207	207	P	1712	tight positional	0.25	0.25
208	208	Q	1729	tight positional	0.24	0.25
209	209	R	1712	tight positional	0.19	0.25
210	210	S	1729	tight positional	0.28	0.25
211	211	T	1729	tight positional	0.24	0.25
212	212	U	1729	tight positional	0.27	0.25
213	213	V	1712	tight positional	0.23	0.25
214	214	W	1729	tight positional	0.24	0.25
215	215	X	1712	tight positional	0.23	0.25
216	216	Y	1712	tight positional	0.22	0.25
217	217	Z	1712	tight positional	0.27	0.25
218	218	A	1712	tight positional	0.24	0.25
219	219	B	1712	tight positional	0.22	0.25
220	220	C	1712	tight positional	0.23	0.25
221	221	D	1720	tight positional	0.25	0.25
222	222	E	1712	tight positional	0.24	0.25
223	223	F	1720	tight positional	0.19	0.25
224	224	G	1712	tight positional	0.24	0.25
225	225	H	1712	tight positional	0.2	0.25
226	226	I	1712	tight positional	0.22	0.25
227	227	J	1720	tight positional	0.24	0.25
228	228	K	1712	tight positional	0.21	0.25
229	229	L	1720	tight positional	0.22	0.25
230	230	M	1720	tight positional	0.21	0.25
231	231	N	1720	tight positional	0.29	0.25
232	232	O	1720	tight positional	0.21	0.25
233	233	P	1720	tight positional	0.22	0.25
234	234	Q	1720	tight positional	0.21	0.25
235	235	R	1712	tight positional	0.26	0.25
236	236	S	1720	tight positional	0.25	0.25
237	237	T	1712	tight positional	0.26	0.25
238	238	U	1712	tight positional	0.24	0.25
239	239	V	1712	tight positional	0.24	0.25
240	240	W	1720	tight positional	0.27	0.25
241	241	X	1712	tight positional	0.24	0.25
242	242	Y	1720	tight positional	0.25	0.25
243	243	Z	1720	tight positional	0.27	0.25
244	244	A	1720	tight positional	0.28	0.25
245	245	B	1720	tight positional	0.25	0.25
246	246	C	1720	tight positional	0.26	0.25
247	247	D	1720	tight positional	0.25	0.25
248	248	E	1712	tight positional	0.22	0.25
249	249	F	1729	tight positional	0.27	0.25
250	250	G	1729	tight positional	0.29	0.25
251	251	H	1729	tight positional	0.28	0.25
252	252	I	1712	tight positional	0.25	0.25
253	253	J	1729	tight positional	0.26	0.25
254	254	K	1712	tight positional	0.23	0.25
255	255	L	1712	tight positional	0.25	0.25
256	256	M	1712	tight positional	0.29	0.25
257	257	N	1712	tight positional	0.25	0.25
258	258	O	1712	tight positional	0.24	0.25
259	259	P	1712	tight positional	0.27	0.25
260	260	Q	1712	tight positional	0.26	0.25
261	261	R	1712	tight positional	0.22	0.25
262	262	S	1712	tight positional	0.22	0.25
263	263	T	1720	tight positional	0.23	0.25
264	264	U	1712	tight positional	0.23	0.25
265	265	V	1720	tight positional	0.23	0.25
266	266	W	1720	tight positional	0.21	0.25
267	267	X	1720	tight positional	0.28	0.25
268	268	Y	1720	tight positional	0.22	0.25
269	269	Z	1720	tight positional	0.22	0.25
270	270	A	1720	tight positional	0.23	0.25
271	271	B	1729	tight positional	0.3	0.25
272	272	C	1729	tight positional	0.33	0.25
273	273	D	1712	tight positional	0.26	0.25
274	274	E	1729	tight positional	0.28	0.25
275	275	F	1712	tight positional	0.23	0.25
276	276	G	1712	tight positional	0.26	0.25
277	277	H	1712	tight positional	0.3	0.25
278	278	I	1712	tight positional	0.25	0.25
279	279	J	1712	tight positional	0.27	0.25
280	280	K	1712	tight positional	0.25	0.25
281	281	L	1729	tight positional	0.29	0.25
282	282	M	1712	tight positional	0.25	0.25
283	283	N	1729	tight positional	0.25	0.25
284	284	O	1712	tight positional	0.25	0.25
285	285	P	1712	tight positional	0.22	0.25
286	286	Q	1712	tight positional	0.28	0.25
287	287	R	1712	tight positional	0.22	0.25
288	288	S	1712	tight positional	0.22	0.25
289	289	T	1712	tight positional	0.21	0.25
290	290	U	1712	tight positional	0.23	0.25
291	291	V	1729	tight positional	0.26	0.25
292	292	W	1712	tight positional	0.24	0.25
293	293	X	1712	tight positional	0.23	0.25
294	294	Y	1712	tight positional	0.24	0.25
295	295	Z	1712	tight positional	0.23	0.25
296	296	A	1712	tight positional	0.2	0.25
297	297	B	1712	tight positional	0.25	0.25
298	298	C	1712	tight positional	0.24	0.25
299	299	D	1720	tight positional	0.23	0.25
300	300	E	1720	tight positional	0.22	0.25
301	301	F	1720	tight positional	0.28	0.25
302	302	G	1720	tight positional	0.24	0.25
303	303	H	1720	tight positional	0.22	0.25
304	304	I	1720	tight positional	0.25	0.25
305	305	J	1712	tight positional	0.2	0.25
306	306	K	1712	tight positional	0.22	0.25
307	307	L	1712	tight positional	0.27	0.25
308	308	M	1712	tight positional	0.21	0.25
309	309	N	1712	tight positional	0.22	0.25
310	310	O	1712	tight positional	0.2	0.25
311	311	P	1720	tight positional	0.22	0.25
312	312	Q	1720	tight positional	0.29	0.25
313	313	R	1720	tight positional	0.22	0.25
314	314	S	1720	tight positional	0.22	0.25
315	315	T	1720	tight positional	0.23	0.25
316	316	U	1720	tight positional	0.28	0.25
317	317	V	1720	tight positional	0.22	0.25
318	318	W	1720	tight positional	0.2	0.25
319	319	X	1720	tight positional	0.23	0.25
320	320	Y	1720	tight positional	0.28	0.25
321	321	Z	1720	tight positional	0.27	0.25
322	322	A	1720	tight positional	0.28	0.25
323	323	B	1720	tight positional	0.22	0.25
324	324	C	1720	tight positional	0.22	0.25
325	325	D	1720	tight positional	0.21	0.25
1	1	E	1720	tight thermal	5.45	2.5
2	2	F	1712	tight thermal	7.14	2.5
3	3	G	1720	tight thermal	13.75	2.5
4	4	H	1720	tight thermal	10.83	2.5
5	5	I	1720	tight thermal	8.71	2.5
6	6	J	1712	tight thermal	7.25	2.5
7	7	K	1712	tight thermal	8.34	2.5
8	8	L	1712	tight thermal	10.43	2.5
9	9	M	1712	tight thermal	7.97	2.5
10	10	N	1712	tight thermal	9.42	2.5
11	11	O	1720	tight thermal	9.76	2.5
12	12	P	1720	tight thermal	6.79	2.5
13	13	Q	1712	tight thermal	8.71	2.5
14	14	R	1720	tight thermal	11.17	2.5
15	15	S	1712	tight thermal	10.67	2.5
16	16	T	1712	tight thermal	7.11	2.5
17	17	U	1712	tight thermal	6.89	2.5
18	18	V	1720	tight thermal	7.82	2.5
19	19	W	1712	tight thermal	7.65	2.5
20	20	X	1720	tight thermal	6.37	2.5
21	21	Y	1720	tight thermal	8.1	2.5
22	22	Z	1720	tight thermal	8.88	2.5
23	23	A	1720	tight thermal	6.72	2.5
24	24	B	1720	tight thermal	10.37	2.5
25	25	C	1720	tight thermal	9.67	2.5
26	26	D	1712	tight thermal	6.59	2.5
27	27	E	1720	tight thermal	13.56	2.5
28	28	F	1720	tight thermal	10.36	2.5
29	29	G	1720	tight thermal	7.84	2.5
30	30	H	1712	tight thermal	6.33	2.5
31	31	I	1712	tight thermal	7.13	2.5
32	32	J	1712	tight thermal	9.66	2.5
33	33	K	1712	tight thermal	7.36	2.5
34	34	L	1712	tight thermal	8.54	2.5
35	35	M	1720	tight thermal	8.91	2.5
36	36	N	1720	tight thermal	6.25	2.5
37	37	O	1712	tight thermal	7.77	2.5
38	38	P	1720	tight thermal	10.48	2.5
39	39	Q	1712	tight thermal	10.27	2.5
40	40	R	1712	tight thermal	6.98	2.5
41	41	S	1712	tight thermal	6.25	2.5
42	42	T	1720	tight thermal	7.93	2.5
43	43	U	1712	tight thermal	7.29	2.5
44	44	V	1720	tight thermal	5.86	2.5
45	45	W	1720	tight thermal	8.13	2.5
46	46	X	1720	tight thermal	8.99	2.5
47	47	Y	1720	tight thermal	6.48	2.5
48	48	Z	1720	tight thermal	9.77	2.5
49	49	A	1720	tight thermal	9.71	2.5
50	50	B	1712	tight thermal	11.81	2.5
51	51	C	1712	tight thermal	8.87	2.5
52	52	D	1712	tight thermal	8.19	2.5
53	53	E	1729	tight thermal	8.57	2.5
54	54	F	1729	tight thermal	9.9	2.5
55	55	G	1729	tight thermal	12.26	2.5
56	56	H	1729	tight thermal	9.51	2.5
57	57	I	1729	tight thermal	11.16	2.5
58	58	J	1712	tight thermal	11.6	2.5
59	59	K	1712	tight thermal	7.54	2.5
60	60	L	1729	tight thermal	10.07	2.5
61	61	M	1712	tight thermal	13.41	2.5
62	62	N	1729	tight thermal	13.15	2.5
63	63	O	1729	tight thermal	7.31	2.5
64	64	P	1729	tight thermal	5.65	2.5
65	65	Q	1712	tight thermal	7.49	2.5
66	66	R	1729	tight thermal	6.34	2.5
67	67	S	1712	tight thermal	6.1	2.5
68	68	T	1712	tight thermal	6.53	2.5
69	69	U	1712	tight thermal	7.43	2.5
70	70	V	1712	tight thermal	6.68	2.5
71	71	W	1712	tight thermal	7.26	2.5
72	72	X	1712	tight thermal	7.82	2.5
73	73	Y	1720	tight thermal	9.3	2.5
74	74	Z	1720	tight thermal	12.25	2.5
75	75	A	1712	tight thermal	16.65	2.5
76	76	B	1712	tight thermal	17.63	2.5
77	77	C	1712	tight thermal	20.47	2.5
78	78	D	1712	tight thermal	18.21	2.5
79	79	E	1712	tight thermal	19.8	2.5
80	80	F	1720	tight thermal	19.7	2.5
81	81	G	1720	tight thermal	15.31	2.5
82	82	H	1712	tight thermal	18.28	2.5
83	83	I	1720	tight thermal	21.47	2.5
84	84	J	1712	tight thermal	21.55	2.5
85	85	K	1712	tight thermal	15.77	2.5
86	86	L	1712	tight thermal	12.42	2.5
87	87	M	1720	tight thermal	9.25	2.5
88	88	N	1712	tight thermal	9.96	2.5
89	89	O	1720	tight thermal	11.48	2.5
90	90	P	1720	tight thermal	9.83	2.5
91	91	Q	1720	tight thermal	9.28	2.5
92	92	R	1720	tight thermal	11.87	2.5
93	93	S	1720	tight thermal	9.64	2.5
94	94	T	1720	tight thermal	10.63	2.5
95	95	U	1720	tight thermal	10.5	2.5
96	96	V	1712	tight thermal	13.15	2.5
97	97	W	1712	tight thermal	14.49	2.5
98	98	X	1712	tight thermal	17.04	2.5
99	99	Y	1712	tight thermal	14.28	2.5
100	100	Z	1712	tight thermal	16.25	2.5
101	101	A	1720	tight thermal	16.61	2.5
102	102	B	1720	tight thermal	11.5	2.5
103	103	C	1712	tight thermal	15.05	2.5
104	104	D	1720	tight thermal	18.26	2.5
105	105	E	1712	tight thermal	18.05	2.5
106	106	F	1712	tight thermal	11.66	2.5
107	107	G	1712	tight thermal	9.35	2.5
108	108	H	1720	tight thermal	7.83	2.5
109	109	I	1712	tight thermal	7.58	2.5
110	110	J	1720	tight thermal	9.25	2.5
111	111	K	1720	tight thermal	8.16	2.5
112	112	L	1720	tight thermal	7.38	2.5
113	113	M	1720	tight thermal	8.19	2.5
114	114	N	1720	tight thermal	7.34	2.5
115	115	O	1720	tight thermal	7.58	2.5
116	116	P	1712	tight thermal	9.68	2.5
117	117	Q	1712	tight thermal	9.52	2.5
118	118	R	1712	tight thermal	11.39	2.5
119	119	S	1712	tight thermal	10.94	2.5
120	120	T	1712	tight thermal	11.89	2.5
121	121	U	1720	tight thermal	11.34	2.5
122	122	V	1720	tight thermal	9.7	2.5
123	123	W	1712	tight thermal	9.52	2.5
124	124	X	1720	tight thermal	12.88	2.5
125	125	Y	1712	tight thermal	13.53	2.5
126	126	Z	1712	tight thermal	9.47	2.5
127	127	A	1712	tight thermal	8.61	2.5
128	128	B	1720	tight thermal	8.23	2.5
129	129	C	1712	tight thermal	8.58	2.5
130	130	D	1720	tight thermal	7.22	2.5
131	131	E	1720	tight thermal	8.78	2.5
132	132	F	1720	tight thermal	9.51	2.5
133	133	G	1720	tight thermal	8.63	2.5
134	134	H	1720	tight thermal	10.09	2.5
135	135	I	1720	tight thermal	10.3	2.5
136	136	J	1729	tight thermal	5.44	2.5
137	137	K	1729	tight thermal	7.72	2.5
138	138	L	1729	tight thermal	6.11	2.5
139	139	M	1729	tight thermal	6.12	2.5
140	140	N	1712	tight thermal	6.53	2.5
141	141	O	1712	tight thermal	6.05	2.5
142	142	P	1729	tight thermal	6.01	2.5
143	143	Q	1712	tight thermal	7.39	2.5
144	144	R	1729	tight thermal	7.25	2.5
145	145	S	1729	tight thermal	6.77	2.5
146	146	T	1729	tight thermal	7.76	2.5
147	147	U	1712	tight thermal	10.77	2.5
148	148	V	1729	tight thermal	10.16	2.5
149	149	W	1712	tight thermal	8	2.5
150	150	X	1712	tight thermal	10.6	2.5
151	151	Y	1712	tight thermal	11.49	2.5
152	152	Z	1712	tight thermal	8.82	2.5
153	153	A	1712	tight thermal	12.53	2.5
154	154	B	1712	tight thermal	12.11	2.5
155	155	C	1729	tight thermal	6.3	2.5
156	156	D	1729	tight thermal	6.53	2.5
157	157	E	1729	tight thermal	5.78	2.5
158	158	F	1712	tight thermal	5.35	2.5
159	159	G	1712	tight thermal	7.36	2.5
160	160	H	1729	tight thermal	5.25	2.5
161	161	I	1712	tight thermal	5.97	2.5
162	162	J	1729	tight thermal	6.75	2.5
163	163	K	1729	tight thermal	8.3	2.5
164	164	L	1729	tight thermal	9.2	2.5
165	165	M	1712	tight thermal	11.71	2.5
166	166	N	1729	tight thermal	11.46	2.5
167	167	O	1712	tight thermal	9.14	2.5
168	168	P	1712	tight thermal	11.9	2.5
169	169	Q	1712	tight thermal	12.78	2.5
170	170	R	1712	tight thermal	9.96	2.5
171	171	S	1712	tight thermal	13.79	2.5
172	172	T	1712	tight thermal	13.83	2.5
173	173	U	1729	tight thermal	6.9	2.5
174	174	V	1729	tight thermal	5.92	2.5
175	175	W	1712	tight thermal	5.11	2.5
176	176	X	1712	tight thermal	9.62	2.5
177	177	Y	1729	tight thermal	5.41	2.5
178	178	Z	1712	tight thermal	6.05	2.5
179	179	A	1729	tight thermal	6.98	2.5
180	180	B	1729	tight thermal	9.93	2.5
181	181	C	1729	tight thermal	11.88	2.5
182	182	D	1712	tight thermal	14.5	2.5
183	183	E	1729	tight thermal	14.12	2.5
184	184	F	1712	tight thermal	11.48	2.5
185	185	G	1712	tight thermal	14.63	2.5
186	186	H	1712	tight thermal	15.61	2.5
187	187	I	1712	tight thermal	12.51	2.5
188	188	J	1712	tight thermal	16.35	2.5
189	189	K	1712	tight thermal	16.3	2.5
190	190	L	1729	tight thermal	5.63	2.5
191	191	M	1712	tight thermal	6.66	2.5
192	192	N	1712	tight thermal	5.94	2.5
193	193	O	1729	tight thermal	6.19	2.5
194	194	P	1712	tight thermal	7.86	2.5
195	195	Q	1729	tight thermal	7.51	2.5
196	196	R	1729	tight thermal	6.89	2.5
197	197	S	1729	tight thermal	8.95	2.5
198	198	T	1712	tight thermal	12.07	2.5
199	199	U	1729	tight thermal	11.34	2.5
200	200	V	1712	tight thermal	9.16	2.5
201	201	W	1712	tight thermal	11.81	2.5
202	202	X	1712	tight thermal	12.83	2.5
203	203	Y	1712	tight thermal	10	2.5
204	204	Z	1712	tight thermal	13.28	2.5
205	205	A	1712	tight thermal	12.93	2.5
206	206	B	1712	tight thermal	4.93	2.5
207	207	C	1712	tight thermal	7.98	2.5
208	208	D	1729	tight thermal	5.75	2.5
209	209	E	1712	tight thermal	5.88	2.5
210	210	F	1729	tight thermal	5.58	2.5
211	211	G	1729	tight thermal	8.54	2.5
212	212	H	1729	tight thermal	10.41	2.5
213	213	I	1712	tight thermal	13.65	2.5
214	214	J	1729	tight thermal	13.18	2.5
215	215	K	1712	tight thermal	10.76	2.5
216	216	L	1712	tight thermal	13.66	2.5
217	217	M	1712	tight thermal	14.81	2.5
218	218	N	1712	tight thermal	11.66	2.5
219	219	O	1712	tight thermal	15.31	2.5
220	220	P	1712	tight thermal	15.26	2.5
221	221	Q	1720	tight thermal	8.89	2.5
222	222	R	1712	tight thermal	5.16	2.5
223	223	S	1720	tight thermal	5.2	2.5
224	224	T	1712	tight thermal	5.98	2.5
225	225	U	1712	tight thermal	9.24	2.5
226	226	V	1712	tight thermal	10.74	2.5
227	227	W	1720	tight thermal	13.74	2.5
228	228	X	1712	tight thermal	13.38	2.5
229	229	Y	1720	tight thermal	10.83	2.5
230	230	Z	1720	tight thermal	13.67	2.5
231	231	A	1720	tight thermal	15.01	2.5
232	232	B	1720	tight thermal	12.09	2.5
233	233	C	1720	tight thermal	15.64	2.5
234	234	D	1720	tight thermal	15.66	2.5
235	235	E	1712	tight thermal	7.68	2.5
236	236	F	1720	tight thermal	10.2	2.5
237	237	G	1712	tight thermal	9.72	2.5
238	238	H	1712	tight thermal	6.25	2.5
239	239	I	1712	tight thermal	6.71	2.5
240	240	J	1720	tight thermal	9.62	2.5
241	241	K	1712	tight thermal	8.77	2.5
242	242	L	1720	tight thermal	7.47	2.5
243	243	M	1720	tight thermal	9.25	2.5
244	244	N	1720	tight thermal	10.07	2.5
245	245	O	1720	tight thermal	7.89	2.5
246	246	P	1720	tight thermal	10.71	2.5
247	247	Q	1720	tight thermal	10.18	2.5
248	248	R	1712	tight thermal	6.54	2.5
249	249	S	1729	tight thermal	7.32	2.5
250	250	T	1729	tight thermal	8.19	2.5
251	251	U	1729	tight thermal	9.41	2.5
252	252	V	1712	tight thermal	12.43	2.5
253	253	W	1729	tight thermal	12.05	2.5
254	254	X	1712	tight thermal	9.61	2.5
255	255	Y	1712	tight thermal	12.15	2.5
256	256	Z	1712	tight thermal	13.41	2.5
257	257	A	1712	tight thermal	10.51	2.5
258	258	B	1712	tight thermal	14.05	2.5
259	259	C	1712	tight thermal	13.98	2.5
260	260	D	1712	tight thermal	5.08	2.5
261	261	E	1712	tight thermal	10.63	2.5
262	262	F	1712	tight thermal	12.72	2.5
263	263	G	1720	tight thermal	15.35	2.5
264	264	H	1712	tight thermal	15.15	2.5
265	265	I	1720	tight thermal	12.46	2.5
266	266	J	1720	tight thermal	15.57	2.5
267	267	K	1720	tight thermal	16.54	2.5
268	268	L	1720	tight thermal	13.49	2.5
269	269	M	1720	tight thermal	17.57	2.5
270	270	N	1720	tight thermal	17.29	2.5
271	271	O	1729	tight thermal	10.14	2.5
272	272	P	1729	tight thermal	12.28	2.5
273	273	Q	1712	tight thermal	15.22	2.5
274	274	R	1729	tight thermal	14.91	2.5
275	275	S	1712	tight thermal	12.34	2.5
276	276	T	1712	tight thermal	15.4	2.5
277	277	U	1712	tight thermal	16.34	2.5
278	278	V	1712	tight thermal	13.06	2.5
279	279	W	1712	tight thermal	17.36	2.5
280	280	X	1712	tight thermal	17	2.5
281	281	Y	1729	tight thermal	7.3	2.5
282	282	Z	1712	tight thermal	10.11	2.5
283	283	A	1729	tight thermal	8.89	2.5
284	284	B	1712	tight thermal	7.73	2.5
285	285	C	1712	tight thermal	9.48	2.5
286	286	D	1712	tight thermal	10.21	2.5
287	287	E	1712	tight thermal	8.57	2.5
288	288	F	1712	tight thermal	10.74	2.5
289	289	G	1712	tight thermal	9.94	2.5
290	290	H	1712	tight thermal	7.82	2.5
291	291	I	1729	tight thermal	6.75	2.5
292	292	J	1712	tight thermal	6.35	2.5
293	293	K	1712	tight thermal	6.55	2.5
294	294	L	1712	tight thermal	7.76	2.5
295	295	M	1712	tight thermal	6.75	2.5
296	296	N	1712	tight thermal	7.84	2.5
297	297	O	1712	tight thermal	8	2.5
298	298	P	1712	tight thermal	6.33	2.5
299	299	Q	1720	tight thermal	6.76	2.5
300	300	R	1720	tight thermal	6.78	2.5
301	301	S	1720	tight thermal	6.26	2.5
302	302	T	1720	tight thermal	6.77	2.5
303	303	U	1720	tight thermal	7.96	2.5
304	304	V	1720	tight thermal	7.94	2.5
305	305	W	1712	tight thermal	6.06	2.5
306	306	X	1712	tight thermal	5.79	2.5
307	307	Y	1712	tight thermal	5.82	2.5
308	308	Z	1712	tight thermal	6.96	2.5
309	309	A	1712	tight thermal	6.81	2.5
310	310	B	1712	tight thermal	6.7	2.5
311	311	C	1720	tight thermal	7.02	2.5
312	312	D	1720	tight thermal	7.85	2.5
313	313	E	1720	tight thermal	6.55	2.5
314	314	F	1720	tight thermal	8.42	2.5
315	315	G	1720	tight thermal	8.32	2.5
316	316	H	1720	tight thermal	6.08	2.5
317	317	I	1720	tight thermal	7.61	2.5
318	318	J	1720	tight thermal	6.78	2.5
319	319	K	1720	tight thermal	6.95	2.5
320	320	L	1720	tight thermal	7.45	2.5
321	321	M	1720	tight thermal	7.43	2.5
322	322	N	1720	tight thermal	7.12	2.5
323	323	O	1720	tight thermal	8.42	2.5
324	324	P	1720	tight thermal	8.03	2.5
325	325	Q	1720	tight thermal	6.68	2.5

LS refinement shell

Resolution: 2.73→2.801 Å / Total num. of bins used: 20

	R_factor	Num. reflection	% reflection
R_free	0.371	433	-
R_work	0.338	8753	-
obs	-	-	86.13 %

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

Version 3 of the EMDB header file is now the official format.
The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi

Thousand views of thousand structures

Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options

+
Open data

-
Basic information

-
Structure visualization

-
Downloads & links

-
Download

-
Validation report

-
Related structure data

-
Links

-
Assembly

-
Components

-
Experimental details

-
Experiment

-
Sample preparation

-
Data collection

-
Processing

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

-
Aug 12, 2020. Covid-19 info

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi

-Movie

-Structure viewers

+Viewer log

-This page

-This web site

-Options

+Open data

-Basic information

-Structure visualization

-Downloads & links

-Download

-Validation report

-Related structure data

-Links

-Assembly

-Components

-Experimental details

-Experiment

-Sample preparation

-Data collection

-Processing

+About Yorodumi

-News

-Feb 9, 2022. New format data for meta-information of EMDB entries

-Aug 12, 2020. Covid-19 info

+Mar 5, 2020. Novel coronavirus structure data

+Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+Jul 12, 2017. Major update of PDB

-Yorodumi

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options

+
Open data

-
Basic information

-
Structure visualization

-
Downloads & links

-
Download

-
Validation report

-
Related structure data

-
Links

-
Assembly

-
Components

-
Experimental details

-
Experiment

-
Sample preparation

-
Data collection

-
Processing

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

-
Aug 12, 2020. Covid-19 info

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi