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- PDB-7qh3: Crystal structure of the anti-sigma factor RsfG from Streptomyces... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7qh3 | ||||||
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Title | Crystal structure of the anti-sigma factor RsfG from Streptomyces tsukubaensis NRRL18488 | ||||||
![]() | RsfG | ||||||
![]() | TRANSCRIPTION / anti-sigma / Streptomyces / sigma | ||||||
Function / homology | DUF1579 domain-containing protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Leite, J.P. / Lourenco, F. / Gales, L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structures of Streptomyces tsukubaensis sigma factor SigG1 and anti-sigma RsfG. Authors: Leite, J.P. / Lourenco, F. / Oliveira, R. / Sousa, S.F. / Mendes, M.V. / Gales, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 119.2 KB | Display | ![]() |
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PDB format | ![]() | 91.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7qh5C C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16351.823 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 42081 / NBRC 108919 / NRRL 18488 / 9993 / Gene: STSU_011565, STSU_11555 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.73 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.1 M Ammonium phosphate dibasic; 0.1 M Tris hydrochloride pH 8.5; 0.5 M sodium phosphate dibasic dehydrate; 0.5 M potassium phosphate dibasic |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 25, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97856 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→70.27 Å / Num. obs: 31236 / % possible obs: 99.5 % / Redundancy: 3.5 % / Biso Wilson estimate: 43.61 Å2 / CC1/2: 0.995 / Net I/σ(I): 8.5 |
Reflection shell | Resolution: 2.3→2.38 Å / Rmerge(I) obs: 0.087 / Num. unique obs: 3091 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 95.75 Å2 / Biso mean: 44.9133 Å2 / Biso min: 22.19 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.3→70.27 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 11
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