詳細 | タイプ | Solution-ID | 内容 | Label | 溶媒系 |
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solution1 | 0.4 mM [U-15N] PA4635, 25 mM sodium phosphate, 150 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O | 15N_sample90% H2O/10% D2Osolution2 | 0.4 mM [U-13C; U-15N] PA4635, 25 mM sodium phosphate, 150 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O | 13C_sample90% H2O/10% D2Osolution3 | 0.4 mM [U-13C; U-15N] PA4635, 25 mM sodium phosphate, 150 mM sodium chloride, 2 mM DTT, 100% D2O | D2O_sample100% D2Osolution4 | 0.4 mM [U-13C; U-15N] PA4635, 25 mM sodium phosphate, 150 mM sodium chloride, 2 mM DTT, 100% D2O | C13_D2O_sample100% D2O | | | | | | | | | | | |
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試料 | 濃度 (mg/ml) | 構成要素 | Isotopic labeling | Solution-ID |
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0.4 mMPA4635[U-15N] | 1 | 25 mMsodium phosphatenatural abundance1 | 150 mMsodium chloridenatural abundance1 | 2 mMDTTnatural abundance1 | 0.4 mMPA4635[U-13C; U-15N] | 2 | 25 mMsodium phosphatenatural abundance2 | 150 mMsodium chloridenatural abundance2 | 2 mMDTTnatural abundance2 | 0.4 mMPA4635[U-13C; U-15N] | 3 | 25 mMsodium phosphatenatural abundance3 | 150 mMsodium chloridenatural abundance3 | 2 mMDTTnatural abundance3 | 0.4 mMPA4635[U-13C; U-15N] | 4 | 25 mMsodium phosphatenatural abundance4 | 150 mMsodium chloridenatural abundance4 | 2 mMDTTnatural abundance4 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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試料状態 | イオン強度: 150 mM / Label: condition_1 / pH: 6.8 / 圧: 1 atm / 温度: 293 K |
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