Details | Type | Solution-ID | Contents | Details | Label | Solvent system |
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solution1 | 0.6 mM BCL-2 6A, 90% H2O/10% D2O | 0.6 mM RNA, 2.0 mM KCl, 90/10% H2O/D2O, pH=7BCL-2 6A mutant_H2O | 90% H2O/10% D2Osolution2 | 0.6 mM BCL-2 6A, 100% D2O | 0.6 mM RNA, 2.0 mM KCl, 100% D2O, pH=7BCL-2 6A mutant_D2O | 100% D2Osolution3 | 0.15 mM [U-13C; U-15N]-Gua BCL-2 6A, 90% H2O/10% D2O | 0.15 mM RNA, 2.0 mM KCl, 90/10% H2O/D2O, pH=7BCL-2 6A mutant_G 13C, 15N-labeled_H2O | 90% H2O/10% D2Osolution4 | 0.4 mM [U-13C; U-15N]-Ade BCL-2 6A, 100% D2O | 0.4 mM RNA, 2.0 mM KCl, 100% D2O, pH=7BCL-2 6A mutant_A 13C, 15N-labeled_D2O | 100% D2Osolution5 | 0.15 mM [U-13C; U-15N]-Gua BCL-2 6A, 100% D2O | 0.15 mM RNA, 2.0 mM KCl, 100% D2O, pH=7BCL-2 6A mutant_G 13C, 15N-labeled_D2O | 100% D2Osolution6 | 0.4 mM [U-13C; U-15N]-Ura BCL-2 6A, 100% D2O | 0.4 mM RNA, 2.0 mM KCl, 100% D2O, pH=7BCL-2 6A mutant_U 13C, 15N-labeled_D2O | 100% D2O | | | | | | | | | | | | | | | | | |
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Sample | Conc. (mg/ml) | Component | Isotopic labeling | Solution-ID |
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0.6 mMBCL-2 6A | natural abundance1 | 0.6 mMBCL-2 6A | natural abundance2 | 0.15 mMBCL-2 6A | [U-13C; U-15N]-Gua | 3 | 0.4 mMBCL-2 6A | [U-13C; U-15N]-Ade | 4 | 0.15 mMBCL-2 6A | [U-13C; U-15N]-Gua | 5 | 0.4 mMBCL-2 6A | [U-13C; U-15N]-Ura | 6 | | | | | | | | |
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Sample conditions | Conditions-ID | Ionic strength | Label | pH | Pressure (kPa) | Temperature (K) |
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1 | 2 mMexchangeable7.0 1 atm298 K2 | 2 mMnon-exchangeable | 7.0 1 atm298 K3 | 2 mMhydrogen bond7.0 1 atm298 K4 | 2 mMconstant-time HSQC of A | 7.0 1 atm298 K5 | 2 mMconstant-time HSQC of G | 7.0 1 atm298 K6 | 2 mMconstant-time HSQC of U | 7.0 1 atm298 K | | | | | | | | | | | | | | | | | | | | | | | | | |
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