詳細 | タイプ | Solution-ID | 内容 | 詳細 | Label | 溶媒系 |
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solution1 | 1.0 mM BCL-2 6A8U17U, 90% H2O/10% D2O | 1.0 mM RNA, 2.0 mM KCl, 90/10% H2O/D2O, pH=7BCL-2 6A8U17U mutant_H2O | 90% H2O/10% D2Osolution2 | 1.0 mM BCL-2 6A8U17U, 100% D2O | 1.0 mM RNA, 2.0 mM KCl, 100% D2O, pH=7BCL-2 6A8U17U mutant_D2O | 100% D2Osolution3 | 0.5 mM G & U 15N-labeled BCL-2 6A8U17U, 90% H2O/10% D2O | 0.5 mM RNA with G&U 15N-labeled, 2.0 mM KCl, 90/10% H2O/D2O, pH=7 | BCL-2 6A8U17U mutant G&U 15N-labeled_H2O | 90% H2O/10% D2Osolution4 | 0.5 mM G & U 15N-labeled BCL-2 6A8U17U, 100% D2O | 0.5 mM RNA with G&U 15N-labeled, 2.0 mM KCl, 100% D2O, pH=7 | BCL-2 6A8U17U mutant G&U 15N-labeled_D2O | 100% D2Osolution5 | 0.5 mM A & C 15N-labeled BCL-2 6A8U17U, 100% D2O | 0.6 mM RNA with A&C 15N-labeled, 2.0 mM KCl, 100% D2O, pH=7 | BCL-2 6A8U17U mutant A&C 15N-labeled_D2O | 100% D2Osolution6 | 0.5 mM A 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O | 0.5 mM RNA with A 13C, 15N-labeled, 2.0 mM KCl, 100% D2O, pH=7 | BCL-2 6A8U17U mutant A 13C, 15N-labeled_D2O | 100% D2Osolution7 | 0.3 mM C 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O | 0.3 mM RNA with C 13C, 15N-labeled, 2.0 mM KCl, 100% D2O, pH=7 | BCL-2 6A8U17U mutant C 13C, 15N-labeled_D2O | 100% D2Osolution8 | 0.3 mM G 13C-labeled BCL-2 6A8U17U, 100% D2O | 0.3 mM RNA with G 13C-labeled, 2.0 mM KCl, 100% D2O, pH=7 | BCL-2 6A8U17U mutant G 13C-labeled_D2O | 100% D2Osolution9 | 0.5 mM U 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O | 0.5 mM RNA with U 13C, 15N-labeled, 2.0 mM KCl, 100% D2O, pH=7 | BCL-2 6A8U17U mutant U 13C, 15N-labeled_D2O | 100% D2O | | | | | | | | | | | | | | | | | | | |
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試料 | 濃度 (mg/ml) | 構成要素 | Isotopic labeling | Solution-ID |
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1.0 mMBCL-2 6A8U17U | natural abundance1 | 1.0 mMBCL-2 6A8U17U | natural abundance2 | 0.5 mMBCL-2 6A8U17U | G & U 15N-labeled | 3 | 0.5 mMBCL-2 6A8U17U | G & U 15N-labeled | 4 | 0.5 mMBCL-2 6A8U17U | A & C 15N-labeled | 5 | 0.5 mMBCL-2 6A8U17U | A 13C, 15N-labeled | 6 | 0.3 mMBCL-2 6A8U17U | C 13C, 15N-labeled | 7 | 0.3 mMBCL-2 6A8U17U | G 13C-labeled | 8 | 0.5 mMBCL-2 6A8U17U | U 13C, 15N-labeled | 9 | | | | | | | | | | | |
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試料状態 | Conditions-ID | イオン強度 | Label | pH | 圧 (kPa) | 温度 (K) |
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1 | 2 mMexchangeable7 | 1 atm298 K2 | 2 mMnon-exchangeable | 7 | 1 atm298 K3 | 2 mMhydrogen bond7 | 1 atm298 K4 | 2 mMlong-range HSQC of G&U | 7 | 1 atm298 K5 | 2 mMlong-range HSQC of A&C | 7 | 1 atm298 K6 | 2 mMconstant-time HSQC of A | 7 | 1 atm298 K7 | 2 mMconstant-time HSQC of C | 7 | 1 atm298 K8 | 2 mMconstant-time HSQC of G | 7 | 1 atm298 K9 | 2 mMconstant-time HSQC of U | 7 | 1 atm298 K | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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