+
データを開く
-
基本情報
登録情報 | データベース: PDB / ID: 7q3d | ||||||
---|---|---|---|---|---|---|---|
タイトル | Structure of the human CPLANE complex | ||||||
![]() |
| ||||||
![]() | PROTEIN TRANSPORT / ciliogenesis / ciliopathies / longin / lipid binding | ||||||
機能・相同性 | ![]() negative regulation of neural crest formation / negative regulation of fibroblast growth factor receptor signaling pathway involved in neural plate anterior/posterior pattern formation / auditory receptor cell morphogenesis / axonemal basal plate / respiratory system development / tongue morphogenesis / regulation of embryonic cell shape / septin cytoskeleton organization / digestive system development / embryonic body morphogenesis ...negative regulation of neural crest formation / negative regulation of fibroblast growth factor receptor signaling pathway involved in neural plate anterior/posterior pattern formation / auditory receptor cell morphogenesis / axonemal basal plate / respiratory system development / tongue morphogenesis / regulation of embryonic cell shape / septin cytoskeleton organization / digestive system development / embryonic body morphogenesis / regulation of ruffle assembly / regulation of fibroblast migration / podocyte cell migration / establishment of planar polarity / spinal cord dorsal/ventral patterning / motile cilium assembly / embryonic skeletal system morphogenesis / circulatory system development / cilium organization / negative regulation of cell division / positive regulation of cilium assembly / non-motile cilium assembly / regulation of smoothened signaling pathway / positive regulation of smoothened signaling pathway / camera-type eye development / motile cilium / regulation of establishment of cell polarity / limb development / neural tube development / regulation of focal adhesion assembly / smoothened signaling pathway / embryonic digit morphogenesis / hair follicle morphogenesis / roof of mouth development / axoneme / hair follicle development / cilium assembly / regulation of ossification / negative regulation of keratinocyte proliferation / Hedgehog 'off' state / vesicle-mediated transport / keratinocyte differentiation / negative regulation of cell migration / ciliary basal body / kidney development / neural tube closure / cell projection / establishment of protein localization / negative regulation of canonical Wnt signaling pathway / cilium / regulation of protein localization / protein transport / nervous system development / cytoskeleton / negative regulation of cell population proliferation / cell surface / extracellular exosome / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.35 Å | ||||||
![]() | Langousis, G. / Cavadini, S. / Kempf, G. / Matthias, P. | ||||||
資金援助 | 1件
| ||||||
![]() | ![]() タイトル: Structure of the ciliogenesis-associated CPLANE complex. 著者: Gerasimos Langousis / Simone Cavadini / Niels Boegholm / Esben Lorentzen / Georg Kempf / Patrick Matthias / ![]() ![]() 要旨: Dysfunctional cilia cause pleiotropic human diseases termed ciliopathies. These hereditary maladies are often caused by defects in cilia assembly, a complex event that is regulated by the ...Dysfunctional cilia cause pleiotropic human diseases termed ciliopathies. These hereditary maladies are often caused by defects in cilia assembly, a complex event that is regulated by the ciliogenesis and planar polarity effector (CPLANE) proteins Wdpcp, Inturned, and Fuzzy. CPLANE proteins are essential for building the cilium and are mutated in multiple ciliopathies, yet their structure and molecular functions remain elusive. Here, we show that mammalian CPLANE proteins comprise a bona fide complex and report the near-atomic resolution structures of the human Wdpcp-Inturned-Fuzzy complex and of the mouse Wdpcp-Inturned-Fuzzy complex bound to the small guanosine triphosphatase Rsg1. Notably, the crescent-shaped CPLANE complex binds phospholipids such as phosphatidylinositol 3-phosphate via multiple modules and a CPLANE ciliopathy mutant exhibits aberrant lipid binding. Our study provides critical structural and functional insights into an enigmatic ciliogenesis-associated complex as well as unexpected molecular rationales for ciliopathies. | ||||||
履歴 |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-
ダウンロード
PDBx/mmCIF形式 | ![]() | 547.5 KB | 表示 | ![]() |
---|---|---|---|---|
PDB形式 | ![]() | 440.5 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 1.2 MB | 表示 | ![]() |
---|---|---|---|---|
文書・詳細版 | ![]() | 1.2 MB | 表示 | |
XML形式データ | ![]() | 46.6 KB | 表示 | |
CIF形式データ | ![]() | 70.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 13789MC ![]() 7q3eC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
---|---|
類似構造データ | 類似検索 - 機能・相同性 ![]() |
-
リンク
-
集合体
登録構造単位 | ![]()
|
---|---|
1 |
|
-
要素
#1: タンパク質 | 分子量: 89649.320 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: O95876 |
---|---|
#2: タンパク質 | 分子量: 108150.633 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: Q9ULD6 |
#3: タンパク質 | 分子量: 45721.949 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: Q9BT04 |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
---|---|
EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-
試料調製
構成要素 | 名称: CPLANE / タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT |
---|---|
由来(天然) | 生物種: ![]() |
由来(組換発現) | 生物種: ![]() ![]() |
緩衝液 | pH: 8 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
-
電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
---|---|
顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 1.04 e/Å2 フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) |
-
解析
EMソフトウェア |
| |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | |||||||||||||||||||||
3次元再構成 | 解像度: 3.35 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 129902 / 対称性のタイプ: POINT | |||||||||||||||||||||
原子モデル構築 | プロトコル: FLEXIBLE FIT / 空間: REAL |