+Open data
-Basic information
Entry | Database: PDB / ID: 7ps8 | ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | NMR Structure of the U3 RNA G-quadruplex | ||||||||||||||||||||||||||||
Components | RNA (5'-R(*Keywords | VIRUS / HIV-1 | Function / homology | : / RNA / RNA (> 10) | Function and homology information Biological species | HIV-1 06TG.HT008 (virus) | Method | SOLUTION NMR / simulated annealing | Authors | Marquevielle, J. / Amrane, S. | Funding support | France, 1items |
Citation | Journal: To Be Published | Title: NMR Structure of the U3 RNA G-quadruplex Authors: Marquevielle, J. / Amrane, S. History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7ps8.cif.gz | 153 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7ps8.ent.gz | 124.2 KB | Display | PDB format |
PDBx/mmJSON format | 7ps8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7ps8_validation.pdf.gz | 360.9 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 7ps8_full_validation.pdf.gz | 433.6 KB | Display | |
Data in XML | 7ps8_validation.xml.gz | 11.3 KB | Display | |
Data in CIF | 7ps8_validation.cif.gz | 16.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ps/7ps8 ftp://data.pdbj.org/pub/pdb/validation_reports/ps/7ps8 | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
---|---|
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: RNA chain | Mass: 7585.560 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) HIV-1 06TG.HT008 (virus) |
---|---|
#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Type: solution / Contents: 1.5 mM 1H rHIVpro3, 90% H2O/10% D2O / Label: rHIVpro3 / Solvent system: 90% H2O/10% D2O |
---|---|
Sample | Conc.: 1.5 mM / Component: rHIVpro3 / Isotopic labeling: 1H |
Sample conditions | Ionic strength: 35 mM / Label: buffer 1X / pH: 6.9 / Pressure: 1 atm / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 4 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 750 / Conformers submitted total number: 10 |