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Open data
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Basic information
Entry | Database: PDB / ID: 7pfh | ||||||
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Title | 2 minute Fe2+ soak structure of SynFtn E141D | ||||||
![]() | Ferritin | ||||||
![]() | METAL BINDING PROTEIN / iron binding protein | ||||||
Function / homology | ![]() bacterial non-heme ferritin / intracellular sequestering of iron ion / ferroxidase activity / ferric iron binding / ferrous iron binding / iron ion transport / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
Model details | structure determined from crystals of SynFtn D65A that have not been soaked in Fe2+ | ||||||
![]() | Hemmings, A.M. / Bradley, J.M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Key carboxylate residues for iron transit through the prokaryotic ferritin Syn Ftn. Authors: Bradley, J.M. / Fair, J. / Hemmings, A.M. / Le Brun, N.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 90.6 KB | Display | ![]() |
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PDB format | ![]() | 69.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 970.7 KB | Display | ![]() |
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Full document | ![]() | 971.2 KB | Display | |
Data in XML | ![]() | 10.8 KB | Display | |
Data in CIF | ![]() | 15.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7pf7C ![]() 7pf8C ![]() 7pf9C ![]() 7pfbC ![]() 7pfgC ![]() 7pfiC ![]() 7pfjC ![]() 7pfkC ![]() 5ouwS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 20218.330 Da / Num. of mol.: 1 / Fragment: ferritin / Mutation: E141D Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: CC9311 / Gene: sync_1539 / Plasmid: pET21a / Production host: ![]() ![]() | ||||||||
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#2: Chemical | #3: Chemical | #4: Chemical | ChemComp-ACT / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.85 Å3/Da / Density % sol: 56.85 % / Mosaicity: 0.051 ° |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 4.6 / Details: 2M NaCl |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 17, 2020 | ||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 | ||||||||||||||||||||||||
Reflection | Resolution: 1.41→53.3 Å / Num. obs: 45787 / % possible obs: 99.7 % / Redundancy: 15 % / Rpim(I) all: 0.011 / Rrim(I) all: 0.047 / Net I/σ(I): 23.3 / Num. measured all: 685729 | ||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5OUW Resolution: 1.5→44.218 Å / SU ML: 0.12 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 15.97 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 73.7 Å2 / Biso mean: 30.7856 Å2 / Biso min: 21.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.5→44.218 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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