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Yorodumi- PDB-7p3o: Solution structures of HIV-1 and SIVmac p6 and their interaction ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7p3o | ||||||
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Title | Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles | ||||||
Components | Gag proteinHIV-1 protease | ||||||
Keywords | VIRAL PROTEIN / HIV-1 / P6 | ||||||
Function / homology | Gag protein p6 / Gag protein p6 / Gag protein Function and homology information | ||||||
Biological species | Human immunodeficiency virus 1 | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Wang, X. / Nonin-Lecomte, S. / Bouaziz, S. | ||||||
Citation | Journal: To Be Published Title: Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles Authors: Wang, X. / Bouaziz, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7p3o.cif.gz | 180.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7p3o.ent.gz | 152.9 KB | Display | PDB format |
PDBx/mmJSON format | 7p3o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p3/7p3o ftp://data.pdbj.org/pub/pdb/validation_reports/p3/7p3o | HTTPS FTP |
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-Related structure data
Related structure data | 7p3mC 7p3pC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5812.297 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human immunodeficiency virus 1 / Gene: gag / Production host: Synthecium (invertebrata) / References: UniProt: E9P083 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution / Contents: 1.0 mM unlabeled HIV-1 p6, 90% H2O/10% D2O / Label: unlabeled / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1.0 mM / Component: HIV-1 p6 / Isotopic labeling: unlabeled |
Sample conditions | Ionic strength: 50 mM / Label: conditions_1 / pH: 4 / Pressure: 1 atm / Temperature: 323 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 2 / Details: CNS | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 10 |