+Open data
-Basic information
Entry | Database: PDB / ID: 7olg | ||||||
---|---|---|---|---|---|---|---|
Title | EB1 bound to MACF peptide | ||||||
Components |
| ||||||
Keywords | PROTEIN BINDING / microtubule / +TIPs / homodimer / Leucine zipper | ||||||
Function / homology | Function and homology information protein localization to astral microtubule / cortical microtubule cytoskeleton / mitotic spindle astral microtubule end / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / The role of GTSE1 in G2/M progression after G2 checkpoint / protein localization to microtubule / RHO GTPases Activate Formins ...protein localization to astral microtubule / cortical microtubule cytoskeleton / mitotic spindle astral microtubule end / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / The role of GTSE1 in G2/M progression after G2 checkpoint / protein localization to microtubule / RHO GTPases Activate Formins / Separation of Sister Chromatids / microtubule plus-end / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / cell projection membrane / Regulation of PLK1 Activity at G2/M Transition / attachment of mitotic spindle microtubules to kinetochore / microtubule plus-end binding / non-motile cilium assembly / microtubule bundle formation / protein localization to centrosome / microtubule organizing center / negative regulation of microtubule polymerization / mitotic spindle pole / cytoplasmic microtubule / establishment of mitotic spindle orientation / microtubule polymerization / regulation of microtubule polymerization or depolymerization / spindle assembly / spindle midzone / positive regulation of microtubule polymerization / ciliary basal body / cell projection / microtubule cytoskeleton / cell migration / microtubule / cell division / focal adhesion / centrosome / protein kinase binding / Golgi apparatus / identical protein binding Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Almeida, T.B. / Barsukov, I.L. | ||||||
Citation | Journal: To Be Published Title: EB1 bound to MACF peptide Authors: Almeida, T.B. / Barsukov, I.L. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7olg.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7olg.ent.gz | 962.2 KB | Display | PDB format |
PDBx/mmJSON format | 7olg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7olg_validation.pdf.gz | 504.3 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 7olg_full_validation.pdf.gz | 3.1 MB | Display | |
Data in XML | 7olg_validation.xml.gz | 307.9 KB | Display | |
Data in CIF | 7olg_validation.cif.gz | 321.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ol/7olg ftp://data.pdbj.org/pub/pdb/validation_reports/ol/7olg | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
---|---|
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 8049.979 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mapre1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q61166 #2: Protein/peptide | Mass: 1283.561 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Type: solution Contents: 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O Label: 15N-13C_sample / Solvent system: 95% H2O/5% D2O | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||
Sample conditions | Ionic strength: 50 mM / Label: conditions_1 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 160 / Conformers submitted total number: 20 |