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Open data
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Basic information
Entry | Database: PDB / ID: 7ol2 | ||||||
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Title | Crystal structure of mouse contactin 1 immunoglobulin domains | ||||||
![]() | Contactin-1 | ||||||
![]() | CELL ADHESION / Zipper / Dimer / Glycoprotein / Immunoglobulin cell adhesion protein Neural cell adhesion protein / Horseshoe | ||||||
Function / homology | ![]() : / : / cell-cell adhesion mediator activity / central nervous system myelin formation / positive regulation of sodium ion transport / Notch signaling pathway / myelination / cerebellum development / locomotory behavior / axon guidance ...: / : / cell-cell adhesion mediator activity / central nervous system myelin formation / positive regulation of sodium ion transport / Notch signaling pathway / myelination / cerebellum development / locomotory behavior / axon guidance / brain development / cell-cell adhesion / positive regulation of neuron projection development / positive regulation of peptidyl-tyrosine phosphorylation / neuron projection development / myelin sheath / gene expression / carbohydrate binding / membrane raft / axon / positive regulation of gene expression / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chataigner, L.M.P. / Janssen, B.J.C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into the contactin 1 - neurofascin 155 adhesion complex. Authors: Chataigner, L.M.P. / Gogou, C. / den Boer, M.A. / Frias, C.P. / Thies-Weesie, D.M.E. / Granneman, J.C.M. / Heck, A.J.R. / Meijer, D.H. / Janssen, B.J.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 247.7 KB | Display | ![]() |
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PDB format | ![]() | 197.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7ok5C ![]() 7ol4C ![]() 2om5S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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Components
#1: Protein | Mass: 66907.609 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Polysaccharide | Source method: isolated from a genetically manipulated source #5: Sugar | ChemComp-NAG / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.23 Å3/Da / Density % sol: 70.95 % |
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Crystal grow | Temperature: 277.15 K / Method: vapor diffusion, sitting drop / pH: 5.9 Details: 20% w/v PEG 3350 and 0.2 M Magnesium nitrate hexahydrate pH 5.9 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 31, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9686 Å / Relative weight: 1 |
Reflection | Resolution: 3.89→108.09 Å / Num. obs: 20221 / % possible obs: 93.9 % / Redundancy: 9.3 % / CC1/2: 0.961 / Net I/σ(I): 4.4 |
Reflection shell | Resolution: 3.89→4.27 Å / Mean I/σ(I) obs: 1.5 / Num. unique obs: 3909 / CC1/2: 0.524 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2OM5 Resolution: 3.89→107.94 Å / SU ML: 0.39 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 26.3 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 201.77 Å2 / Biso mean: 105.278 Å2 / Biso min: 39.79 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.89→107.94 Å
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Refine LS restraints NCS |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 7
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