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- PDB-7ogs: X-ray Structure of Interferon Regulatory Factor 4 DNA binding dom... -

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Basic information

Entry
Database: PDB / ID: 7ogs
TitleX-ray Structure of Interferon Regulatory Factor 4 DNA binding domain bound to an interferon-stimulated response element
Components
  • DNA (5'-D(P*AP*TP*AP*AP*CP*TP*GP*AP*AP*AP*CP*CP*GP*AP*AP*AP*GP*TP*AP*C)-3')
  • DNA (5'-D(P*TP*GP*TP*AP*CP*TP*TP*TP*CP*GP*GP*TP*TP*TP*CP*AP*GP*TP*TP*A)-3')
  • Interferon regulatory factor 4
KeywordsDNA BINDING PROTEIN / INTERFERON REGULATORY FACTORS / TRANSCRIPTION REGULATION / PROTEIN-DNA INTERACTION / TRANSCRIPTION
Function / homology
Function and homology information


T-helper 17 cell lineage commitment / regulation of T-helper cell differentiation / negative regulation of toll-like receptor signaling pathway / positive regulation of interleukin-13 production / myeloid dendritic cell differentiation / defense response to protozoan / positive regulation of interleukin-4 production / Regulation of MITF-M-dependent genes involved in pigmentation / positive regulation of interleukin-10 production / immune system process ...T-helper 17 cell lineage commitment / regulation of T-helper cell differentiation / negative regulation of toll-like receptor signaling pathway / positive regulation of interleukin-13 production / myeloid dendritic cell differentiation / defense response to protozoan / positive regulation of interleukin-4 production / Regulation of MITF-M-dependent genes involved in pigmentation / positive regulation of interleukin-10 production / immune system process / Nuclear events stimulated by ALK signaling in cancer / positive regulation of interleukin-2 production / T cell activation / Interferon gamma signaling / sequence-specific double-stranded DNA binding / Interferon alpha/beta signaling / nucleosome / positive regulation of cold-induced thermogenesis / DNA-binding transcription activator activity, RNA polymerase II-specific / Interleukin-4 and Interleukin-13 signaling / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / transcription coactivator activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin remodeling / DNA-binding transcription factor activity / regulation of transcription by RNA polymerase II / chromatin / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / membrane / cytosol
Similarity search - Function
Interferon regulatory factor-3 / Interferon-regulatory factor 3 / Interferon-regulatory factor 3 / Interferon regulatory factor, conserved site / IRF tryptophan pentad repeat DNA-binding domain signature. / Interferon regulatory factor transcription factor / interferon regulatory factor / Interferon regulatory factor DNA-binding domain / IRF tryptophan pentad repeat DNA-binding domain profile. / SMAD-like domain superfamily ...Interferon regulatory factor-3 / Interferon-regulatory factor 3 / Interferon-regulatory factor 3 / Interferon regulatory factor, conserved site / IRF tryptophan pentad repeat DNA-binding domain signature. / Interferon regulatory factor transcription factor / interferon regulatory factor / Interferon regulatory factor DNA-binding domain / IRF tryptophan pentad repeat DNA-binding domain profile. / SMAD-like domain superfamily / SMAD/FHA domain superfamily / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
DNA / DNA (> 10) / Interferon regulatory factor 4
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.37 Å
AuthorsAgnarelli, A. / El Omari, K. / Alt, A.O. / Mancini, E.J.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Wellcome Trust204833/Z/16/Z United Kingdom
CitationJournal: To Be Published
Title: X-ray Structure of Interferon Regulatory Factor 4 DNA binding domain bound to interferon-stimulated response element
Authors: Agnarelli, A. / El Omari, K. / Alt, A.O. / Mancini, E.J.
History
DepositionMay 7, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 1, 2022Provider: repository / Type: Initial release
Revision 1.1Jun 22, 2022Group: Derived calculations / Category: pdbx_struct_assembly / pdbx_struct_assembly_gen
Revision 1.2Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Interferon regulatory factor 4
B: Interferon regulatory factor 4
C: DNA (5'-D(P*TP*GP*TP*AP*CP*TP*TP*TP*CP*GP*GP*TP*TP*TP*CP*AP*GP*TP*TP*A)-3')
D: DNA (5'-D(P*AP*TP*AP*AP*CP*TP*GP*AP*AP*AP*CP*CP*GP*AP*AP*AP*GP*TP*AP*C)-3')
E: Interferon regulatory factor 4
F: Interferon regulatory factor 4
G: DNA (5'-D(P*TP*GP*TP*AP*CP*TP*TP*TP*CP*GP*GP*TP*TP*TP*CP*AP*GP*TP*TP*A)-3')
H: DNA (5'-D(P*AP*TP*AP*AP*CP*TP*GP*AP*AP*AP*CP*CP*GP*AP*AP*AP*GP*TP*AP*C)-3')


Theoretical massNumber of molelcules
Total (without water)89,7568
Polymers89,7568
Non-polymers00
Water00
1
A: Interferon regulatory factor 4
B: Interferon regulatory factor 4
C: DNA (5'-D(P*TP*GP*TP*AP*CP*TP*TP*TP*CP*GP*GP*TP*TP*TP*CP*AP*GP*TP*TP*A)-3')
D: DNA (5'-D(P*AP*TP*AP*AP*CP*TP*GP*AP*AP*AP*CP*CP*GP*AP*AP*AP*GP*TP*AP*C)-3')


Theoretical massNumber of molelcules
Total (without water)44,8784
Polymers44,8784
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
E: Interferon regulatory factor 4
F: Interferon regulatory factor 4
G: DNA (5'-D(P*TP*GP*TP*AP*CP*TP*TP*TP*CP*GP*GP*TP*TP*TP*CP*AP*GP*TP*TP*A)-3')
H: DNA (5'-D(P*AP*TP*AP*AP*CP*TP*GP*AP*AP*AP*CP*CP*GP*AP*AP*AP*GP*TP*AP*C)-3')


Theoretical massNumber of molelcules
Total (without water)44,8784
Polymers44,8784
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)64.347, 66.082, 202.195
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "A"
d_2ens_1chain "B"
d_3ens_1chain "E"
d_4ens_1chain "F"
d_1ens_2chain "C"
d_2ens_2chain "G"
d_1ens_3chain "D"
d_2ens_3chain "H"

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1GLYLYSA1 - 114
d_21ens_1GLYLYSB1 - 114
d_31ens_1GLYLYSE1 - 114
d_41ens_1GLYLYSF1 - 114
d_11ens_2DTDAC
d_21ens_2DTDAG
d_11ens_3DADCD
d_21ens_3DADCH

NCS ensembles :
ID
ens_1
ens_2
ens_3

NCS oper:
IDCodeMatrixVector
1given(0.99453475912, -0.103254429535, -0.0154640125405), (-0.0978611635577, -0.870293244491, -0.482714057451), (0.0363841389839, 0.481589235111, -0.87564148089)-18.8087007136, 4.11999216151, 66.793283972
2given(0.997823556697, 0.0574988154849, 0.0322805811198), (0.0606694813989, -0.99231809052, -0.107814754336), (0.0258333839516, 0.109538547752, -0.993646789775)-43.1316420143, -31.6989155292, 50.9149607993
3given(0.99485555124, -0.101121541181, -0.00607174415108), (0.0831999198696, 0.781404319767, 0.618453767378), (-0.0577945109981, -0.615777332288, 0.785797729406)-61.8312274785, -45.8778483404, -14.8208223584
4given(0.999930554592, 0.0103331274992, -0.0056667865785), (0.00958751098265, -0.992882092211, -0.118714913136), (-0.00685314724765, 0.118652338551, -0.992912210082)-42.1425441033, -31.9113635106, 50.4378293184
5given(0.999813651277, 0.0189917629705, 0.00346058663212), (0.019283125693, -0.990958322639, -0.132777120982), (0.000907625514726, 0.132819109062, -0.991139879373)-42.4301638482, -31.2924202864, 50.6793973265

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Components

#1: Protein
Interferon regulatory factor 4 / IRF-4 / Lymphocyte-specific interferon regulatory factor / LSIRF / Multiple myeloma oncogene 1 / NF-EM5


Mass: 16306.470 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: IRF4, MUM1 / Plasmid: pCDFDUET / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: Q15306
#2: DNA chain DNA (5'-D(P*TP*GP*TP*AP*CP*TP*TP*TP*CP*GP*GP*TP*TP*TP*CP*AP*GP*TP*TP*A)-3')


Mass: 6120.960 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#3: DNA chain DNA (5'-D(P*AP*TP*AP*AP*CP*TP*GP*AP*AP*AP*CP*CP*GP*AP*AP*AP*GP*TP*AP*C)-3')


Mass: 6144.034 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.39 Å3/Da / Density % sol: 48.64 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.5
Details: 0.1M Bis Tris pH 5.5, 0.2M ammonium acetate, 25% PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.9119 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jan 26, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9119 Å / Relative weight: 1
ReflectionResolution: 2.37→64.35 Å / Num. obs: 35957 / % possible obs: 99.99 % / Redundancy: 6.3 % / Biso Wilson estimate: 63.07 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.174 / Rpim(I) all: 0.074 / Rrim(I) all: 0.189 / Net I/σ(I): 5.8
Reflection shellResolution: 2.37→2.41 Å / Redundancy: 5.5 % / Rmerge(I) obs: 3.086 / Mean I/σ(I) obs: 0.2 / Num. unique obs: 1740 / CC1/2: 0.281 / Rpim(I) all: 1.434 / Rrim(I) all: 3.409 / % possible all: 100

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Processing

Software
NameVersionClassification
xia2data reduction
xia2data scaling
PHASERphasing
PHENIX1.18.2_3874refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7O56

7o56


Resolution: 2.37→62.81 Å / SU ML: 0.5164 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 34.5193
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2494 1724 4.95 %
Rwork0.2167 33082 -
obs0.2183 34806 96.97 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 76.85 Å2
Refinement stepCycle: LAST / Resolution: 2.37→62.81 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3792 1640 0 0 5432
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00995732
X-RAY DIFFRACTIONf_angle_d1.25088076
X-RAY DIFFRACTIONf_chiral_restr0.0655832
X-RAY DIFFRACTIONf_plane_restr0.0079764
X-RAY DIFFRACTIONf_dihedral_angle_d30.01122284
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.296299807953
ens_1d_3AX-RAY DIFFRACTIONTorsion NCS0.25241330114
ens_1d_4AX-RAY DIFFRACTIONTorsion NCS0.276051849632
ens_2d_2CX-RAY DIFFRACTIONTorsion NCS0.583357700148
ens_3d_2DX-RAY DIFFRACTIONTorsion NCS0.550695991247
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.37-2.440.41291040.43791983X-RAY DIFFRACTION71.55
2.44-2.520.40221250.42392751X-RAY DIFFRACTION98.32
2.52-2.610.40911820.38852752X-RAY DIFFRACTION99.86
2.61-2.710.37971510.37712821X-RAY DIFFRACTION99.83
2.71-2.840.41141550.38892762X-RAY DIFFRACTION99.59
2.84-2.990.36211400.33532821X-RAY DIFFRACTION99.6
2.99-3.170.30081470.27892758X-RAY DIFFRACTION97.84
3.17-3.420.29991230.26212792X-RAY DIFFRACTION97
3.42-3.760.25261400.21722833X-RAY DIFFRACTION99.77
3.76-4.310.20951390.17792866X-RAY DIFFRACTION99.73
4.31-5.420.21321550.15852910X-RAY DIFFRACTION99.97
5.43-62.810.16111630.13013033X-RAY DIFFRACTION99.81
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.14436183020.557245651818-0.2971518564373.09861312174-0.669132370070.8252967943520.1754561940730.380306228390.123914539178-0.6226183951710.09188086125080.692279521997-0.648673467824-0.3735866310930.06429883197660.860883680613-0.0855628297808-0.09244946860470.5037717252880.07155296781530.54076609881-16.186334805510.230689360216.4368273818
22.047922488410.4157042441371.841177547362.723073666571.991387868873.51999052843-0.590305752984-0.1342425656280.286841124802-0.1747697202730.308994203555-0.943672026323-1.306450226150.602490410960.04940112041650.801527937113-0.1998508817260.01110135327950.6389951126180.04128408269070.828373846696-1.856742356497.5515134040624.515731168
32.80206346609-0.1057253377030.3590253614960.581764356006-0.7673329823851.976549778880.117944948486-0.7296092178260.2501435135370.488566027556-0.223784756273-0.0487789022036-0.1052757184780.1478864669630.09143892477880.699920982566-0.1244001953820.01315092250260.503522740098-0.06291336020420.573570409014-12.87590911847.0493648065432.6843642785
44.61587679833-1.1024384197-1.147805461342.72329793111-0.0440187005073.715110874880.3608240234550.86880649386-0.731397226055-0.697202618081-0.178051294405-0.7528007367250.4285086644190.36267616479-0.01431870988810.929366716539-0.07949356695770.08169174732170.684535900332-0.02339478798360.712226438128-8.34579972585-0.46928084283215.6973574014
50.0496056529631-0.146471168693-0.08156338644560.5882302517620.5523554548950.774212024068-0.2696506871940.3749492557570.983280035844-0.6160641062890.0675857751867-0.2028465972980.1813578258790.633237374496-0.1346175189111.02192859478-0.170585681082-0.02156072050061.174662100920.1341755069411.27087523902-25.757913492-1.7306172675813.1159220872
64.30393542885-0.09969172102141.575922366881.88416685574-0.4913862866740.7061238918950.279538451559-0.53770068803-0.05051745372760.114310578949-0.3734542846480.2532949350090.594334069182-0.75179120848-0.06366319698650.605965467167-0.08657871376730.02042572533420.7723483035570.01072294200640.633384734135-36.2191640683-11.123849725456.7725426902
71.95391622716-1.02077142773-1.776243626054.851853784562.94622691362.89733300262-0.320487828746-1.54199817586-0.250652009629-0.1868322116870.580261691612-0.3084062811150.05274667114650.90705142396-0.1926374118680.588816070389-0.00312866614792-0.09708564413320.68994428614-0.01461940299270.532325013781-21.2917803087-11.949374778849.2613590896
89.22449267685-0.206222134823-1.347327533983.02037619014-2.697162817772.918144692480.417904009967-0.701881232579-1.139064684320.136257312768-0.1147446536940.135289803004-0.4868003999050.551352276734-0.3231806490640.628354419297-0.0461910852171-0.09203812063020.607368108769-0.02792883637210.783313671925-23.3626490766-20.973564807847.6701642641
91.85854172374-0.365494413762-0.2805521047591.62982999990.3964027179431.84276412648-0.00582948735113-0.10801901322-0.0773593163299-0.3861843761390.08259044414580.1044676037490.0136763211678-0.132835826871-0.07839205260850.566268181956-0.0642215645108-0.09083401875560.5522882569380.04890797109820.539986404753-32.8313168011-16.52867996441.0724226527
104.495349127790.5203660672461.681459610843.18370481310.6469286896473.32594161052-0.316581797616-0.8572848354110.8895049973390.3532878203360.247330109851-0.231446757615-0.462976181973-0.07414824006440.0006788886161440.639575974447-0.0364731708558-0.03175484607770.612500123101-0.01925189636780.564877430462-27.3214209465-2.2160798797852.5545323358
110.156434747417-0.0453040199562-0.1262357797240.006208511653340.03026173976530.0881969018538-0.205095947584-0.201850471141-0.258403626138-0.5867744859691.08009688858-0.1762430454420.1533351916620.710378948398-0.09355324284230.7419510655970.1132895738860.003350733605631.15681885817-0.2515297803921.42311087145-44.5032008261.9407763996653.4560200414
123.85957065952-0.563959367106-2.537750055299.951459503922.283145902932.90606696272-0.7335585690131.944283624111.703491183370.006817085226570.07728856903571.10923699928-1.10155534778-0.40897590704-0.09194959826430.4377628674-0.0159268394506-0.1593727660381.240735994330.1404706620971.03319540259-49.0137250972-3.2550204358138.1784143177
133.572822139852.98097817596-2.011195870652.70673789466-0.8921246838632.39008554646-0.0867481884201-0.12739784591-0.425353099899-0.0165745844821-0.0523136390419-0.402307953525-0.003846791477920.1969901770290.1094095363670.513437371583-0.0727387192692-0.04628699085850.705430870241-0.001965109718620.685542562556-16.643836174-7.0518588400732.7967225523
141.282653551640.3449243790231.699958250262.663249997173.7528635566.56808094802-0.103901274648-2.0162857395-0.9434651237880.864661506139-0.137513608855-1.14954070778-1.613556093511.555600788920.6147557829750.427666452568-0.244233841448-0.1681794025021.389130687180.3469809299061.21400459983.54940087935-3.794752159337.0380093306
153.160620268821.04601564492-0.4607873199172.9927648139-1.311588293773.17732880511-0.3563264304440.438929752859-0.188607263765-0.334491935350.1706361405750.0452514057858-0.0313942054312-0.3283285055560.1638041052810.433611621315-0.0833166088465-0.08574191097760.523472077909-0.0245022947220.478769177486-28.5953014176-6.5009438354532.9663151603
164.32044507481-0.2581937026521.348705797242.06278335084-0.3530657217811.19943526870.263004036261-0.829872495383-0.8529718427950.006401827466920.2918998307270.3413698311330.837375206495-0.745021214245-0.00176966946080.69123189141-0.113480914312-0.06967497109830.6874702784150.1417564741890.622244144663-58.1609153534-44.593459553835.2896842511
172.40249347941-1.22170650567-1.282680025653.432031313651.722226972153.30373222118-0.415058562644-0.788344637475-1.25075246513-0.309859894445-0.0190303173573-0.5115304092660.8071450717280.933829996972-0.144704343450.7291885198590.03991445819950.03518325838130.7457039180450.07381882888550.852085480974-43.7225739453-41.969186271927.3073346643
182.91008834949-0.428641190833-0.215355919970.538621077611-0.8773089855762.670128633060.2260664304660.79192630898-0.595953349033-0.615494087046-0.334774132006-0.1192129910750.33164383515-0.152187382293-0.05119426079190.705859262203-0.143861592117-0.03234369652850.639849823215-0.1370538230520.647592112967-54.4921700466-42.964254604318.8806659852
194.684845769990.2581169294211.69784805772.9278524021.102519666113.25689647930.33928403108-0.8388760587690.7248840939510.343471694874-0.000612716534102-0.201419696153-0.322509992004-0.05059934062060.02154737071490.648371281428-0.136239612049-0.05540087101220.5931611847520.01163148635860.561055601223-51.0460551945-33.491457271335.0528450528
200.192335319958-0.216192306681-0.2928234680610.5955222527160.4044960832290.4668377526430.377006712873-0.558157079922-0.7334331681320.9995835025110.972901854479-0.442802550401-0.928999002640.533632931203-0.1936195214540.945117701859-0.02042915814390.02671159351471.494672604590.08095806338211.57401232353-68.5238987664-32.886838710237.0600447727
214.413198016990.114774811725-0.5366178395351.4907384792-0.5593256937321.23248523190.08814541992690.8391030172570.224584760037-0.681058903676-0.1712703515860.214019511511-0.0300998236145-0.3764046487440.1199805277121.035806764870.176902854234-0.1833675438190.952100778993-0.04732104593220.533860759911-79.1068914838-29.0434298366-7.27170927759
223.39274232308-0.05567462755451.493165709142.573698984370.3067198007992.608866052170.04229124611650.5944840052890.385809287472-0.419766287019-0.453138314712-0.148187053846-0.4727759818890.348296656917-0.03835724084910.753310227750.0361723778399-0.0458611440820.7032776496390.01182858030460.543591351574-71.3782020744-22.63485385234.47967449909
234.70501407091-0.681263478532-0.7823414565872.970455514170.3296252792193.11484361980.01161090234391.26589076708-1.23911250983-0.512290520303-0.238505069022-0.1904286935450.9490081224470.9071842724640.02938346160471.050768626740.222754027302-0.02289482320510.934814181246-0.2202670650470.783239840428-70.21372171-37.2439566721-1.69493693692
240.0190859536817-0.007679637015570.084977617560.11271183563-0.1076758794420.4138814016530.3551491023471.21125752361-0.848763623112-0.2553774124640.4050837905880.389097591620.8448872153730.962070528683-0.08459137624031.23435647641-0.0272675957287-0.3067121543061.23913446137-0.2124096241231.36751981712-87.4175556598-41.3575861606-1.94504183545
252.768035758781.073167758912.290866281062.16818312880.153069981993.41811333028-0.612339185638-1.25465417429-1.7590353996-0.3854299480610.226920249770.4621782122681.668513753-0.3159544742680.2485942752960.593633806646-0.07675496958460.01620007900981.193722608180.08978476848481.27427217908-91.4068042876-33.291750473412.6286564876
264.10042799857-0.9975348439873.169780515872.33944134419-0.1650893583162.1971727914-0.05562653541840.1700945764540.730299115512-0.0836154839393-0.143067286258-0.481913628892-0.1177137405570.05538783404310.1250736160950.485011987276-0.0818733822643-0.05648594772910.7088935692660.06114373342030.648760960051-59.0334245378-29.089512147517.0997139496
271.388168412080.089004935463-0.1641808006965.777342211524.255204959963.993266389420.06617707050132.505752455051.00358487543-0.79050080913-0.624355254416-1.574097560330.7656088629381.295719910650.3066655966940.5190686817970.1121117530140.08666568096011.444110289930.2807472513540.962049593587-38.6325114992-32.130632133713.5999715788
283.60939409609-2.703406358691.159103661982.624529253990.1551540274352.48542011646-0.228157692115-0.2423657851230.380519349030.2813110096690.02786848832320.3229420023684.77020830125E-5-0.2781122537170.1894233810670.520113727895-0.03761548522770.01358410153480.654039884974-0.03485993827040.576867351093-71.0908483938-29.857451111317.0744375827
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 20 through 48 )AA20 - 481 - 29
22chain 'A' and (resid 49 through 68 )AA49 - 6830 - 49
33chain 'A' and (resid 69 through 103 )AA69 - 10350 - 84
44chain 'A' and (resid 104 through 127 )AA104 - 12785 - 108
55chain 'A' and (resid 128 through 133 )AA128 - 133109 - 114
66chain 'B' and (resid 20 through 48 )BB20 - 481 - 29
77chain 'B' and (resid 49 through 63 )BB49 - 6330 - 44
88chain 'B' and (resid 64 through 68 )BB64 - 6845 - 49
99chain 'B' and (resid 69 through 103 )BB69 - 10350 - 84
1010chain 'B' and (resid 104 through 127 )BB104 - 12785 - 108
1111chain 'B' and (resid 128 through 133 )BB128 - 133109 - 114
1212chain 'C' and (resid 1 through 5 )CC1 - 5
1313chain 'C' and (resid 6 through 20 )CC6 - 20
1414chain 'D' and (resid 1 through 5 )DD1 - 5
1515chain 'D' and (resid 6 through 20 )DD6 - 20
1616chain 'E' and (resid 20 through 48 )EE20 - 481 - 29
1717chain 'E' and (resid 49 through 68 )EE49 - 6830 - 49
1818chain 'E' and (resid 69 through 103 )EE69 - 10350 - 84
1919chain 'E' and (resid 104 through 127 )EE104 - 12785 - 108
2020chain 'E' and (resid 128 through 133 )EE128 - 133109 - 114
2121chain 'F' and (resid 20 through 48 )FF20 - 481 - 29
2222chain 'F' and (resid 49 through 103 )FF49 - 10330 - 84
2323chain 'F' and (resid 104 through 127 )FF104 - 12785 - 108
2424chain 'F' and (resid 128 through 133 )FF128 - 133109 - 114
2525chain 'G' and (resid 1 through 5 )GG1 - 5
2626chain 'G' and (resid 6 through 20 )GG6 - 20
2727chain 'H' and (resid 1 through 5 )HH1 - 5
2828chain 'H' and (resid 6 through 20 )HH6 - 20

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