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- PDB-7ng3: Crystal structure of MG-132 covalently bound to the main protease... -

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Basic information

Entry
Database: PDB / ID: 7ng3
TitleCrystal structure of MG-132 covalently bound to the main protease (3CLpro/Mpro) of SARS-CoV-2 in spacegroup P1.
Components3C-like proteinase
KeywordsVIRAL PROTEIN / SARS-CoV-2 / Mpro / 3CLpro / EXSCALATE4COV / drug discovery / Elettra / MG-132
Function / homology
Function and homology information


protein guanylyltransferase activity / RNA endonuclease activity producing 3'-phosphomonoesters, hydrolytic mechanism / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Maturation of replicase proteins / TRAF3-dependent IRF activation pathway / ISG15-specific peptidase activity ...protein guanylyltransferase activity / RNA endonuclease activity producing 3'-phosphomonoesters, hydrolytic mechanism / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / Lyases; Phosphorus-oxygen lyases / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Maturation of replicase proteins / TRAF3-dependent IRF activation pathway / ISG15-specific peptidase activity / Transcription of SARS-CoV-2 sgRNAs / snRNP Assembly / Translation of Replicase and Assembly of the Replication Transcription Complex / Replication of the SARS-CoV-2 genome / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / host cell endoplasmic reticulum-Golgi intermediate compartment / double membrane vesicle viral factory outer membrane / SARS coronavirus main proteinase / 5'-3' DNA helicase activity / 3'-5'-RNA exonuclease activity / host cell endosome / symbiont-mediated degradation of host mRNA / mRNA guanylyltransferase / symbiont-mediated suppression of host ISG15-protein conjugation / G-quadruplex RNA binding / symbiont-mediated suppression of host toll-like receptor signaling pathway / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / omega peptidase activity / SARS-CoV-2 modulates host translation machinery / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / host cell Golgi apparatus / symbiont-mediated suppression of host NF-kappaB cascade / symbiont-mediated perturbation of host ubiquitin-like protein modification / DNA helicase / methyltransferase cap1 activity / ubiquitinyl hydrolase 1 / cysteine-type deubiquitinase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / single-stranded RNA binding / regulation of autophagy / host cell perinuclear region of cytoplasm / viral protein processing / lyase activity / host cell endoplasmic reticulum membrane / RNA helicase / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / symbiont-mediated suppression of host gene expression / copper ion binding / viral translational frameshifting / symbiont-mediated activation of host autophagy / RNA-directed RNA polymerase / cysteine-type endopeptidase activity / viral RNA genome replication / RNA-directed RNA polymerase activity / DNA-templated transcription / lipid binding / host cell nucleus / SARS-CoV-2 activates/modulates innate and adaptive immune responses / ATP hydrolysis activity / proteolysis / RNA binding / zinc ion binding / ATP binding / membrane
Similarity search - Function
main proteinase (3clpro) structure, domain 3 / main proteinase (3clpro) structure, domain 3 / RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP14, guanine-N7-methyltransferase domain, coronavirus / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. ...main proteinase (3clpro) structure, domain 3 / main proteinase (3clpro) structure, domain 3 / RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 14, betacoronavirus / NSP15, NendoU domain, coronavirus / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP14, guanine-N7-methyltransferase domain, coronavirus / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / NSP12 RNA-dependent RNA polymerase, coronavirus / : / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Coronavirus Nsp12 Interface domain profile. / : / : / Coronavirus Nonstructural protein 13, 1B domain / Coronavirus Non-structural protein 13, zinc-binding domain / Coronavirus Nonstructural protein 13, stalk domain / Nidovirus 2-O-methyltransferase / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Non-structural protein NSP15, N-terminal domain superfamily, coronavirus / Non-structural protein NSP15, middle domain superfamily / Coronavirus replicase NSP15, N-terminal oligomerization / Nonstructural protein 15, middle domain, coronavirus / Nonstructural protein 13, 1B domain, coronavirus / Coronavirus replicase NSP15, middle domain / Coronavirus replicase NSP15, N-terminal oligomerisation / Nidovirus 3'-5' exoribonuclease domain / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Non-structural protein NSP16, coronavirus-like / Non-structural protein 14, coronavirus / RNA polymerase, N-terminal, coronavirus / Coronavirus 2'-O-methyltransferase / Coronavirus proofreading exoribonuclease / Coronavirus RNA-dependent RNA polymerase, N-terminal / Nonstructural protein 13, zinc-binding domain, coronavirus-like / Coronaviridae zinc-binding (CV ZBD) domain profile. / Arterivirus Nsp11 N-terminal/Coronavirus NSP15 middle domain / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. / Nidoviral uridylate-specific endoribonuclease (NendoU) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Endoribonuclease EndoU-like / NendoU domain, nidovirus / Coronavirus replicase NSP15, uridylate-specific endoribonuclease / : / Lipocalin signature. / DNA2/NAM7 helicase-like, C-terminal / AAA domain / (+) RNA virus helicase core domain / (+)RNA virus helicase core domain profile. / Non-structural protein NSP3, SUD-N (Mac2) domain, betacoronavirus / Sarbecovirus Nsp3c-N domain profile. / Non-structural protein NSP3, N-terminal, betacoronavirus / Polyprotein cleavage domain PL2pro superfamily, betacoronavirus / Non-structural protein NSP3, SUD-N (Mac2) domain superfamily, betacoronavirus / Betacoronavirus SUD-C domain / Betacoronavirus replicase NSP3, N-terminal / NSP1 globular domain superfamily, betacoronavirus / Non-structural protein 2, SARS-CoV-like / Trypsin-like serine proteases / Carbamoyl-phosphate synthase subdomain signature 2. / Betacoronavirus Nsp3c-M domain profile. / NSP1, globular domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain, betacoronavirus / Non-structural protein NSP3, SUD-M domain superfamily, betacoronavirus / Betacoronavirus replicase NSP1 / Betacoronavirus single-stranded poly(A) binding domain / NSP1, C-terminal domain, betacoronavirus / : / Betacoronavirus (BetaCoV) Nsp1 C-terminal domain profile. / Betacoronavirus Nsp3e group 2-specific marker (G2M) domain profile. / Betacoronavirus Nsp3c-C domain profile. / Betacoronavirus Nsp3e nucleic acid-binding (NAB) domain profile. / DPUP/SUD, C-terminal, betacoronavirus / Non-structural protein NSP3, nucleic acid-binding domain, betacoronavirus / Non-structural protein NSP3A domain-like superfamily / Non-structural protein NSP3, nucleic acid-binding domain superfamily, betacoronavirus / Non-structural protein 6, betacoronavirus / Betacoronavirus nucleic acid-binding (NAB) / Papain-like viral protease, palm and finger domains, coronavirus / Papain-like protease, N-terminal domain superfamily, coronavirus / Coronavirus replicase NSP2, N-terminal / : / Coronavirus replicase NSP2, C-terminal / Coronavirus (CoV) Nsp2 middle domain profile. / NSP1, globular domain, alpha/betacoronavirus / Coronavirus (CoV) Nsp1 globular domain profile. / Coronavirus (CoV) Nsp2 N-terminal domain profile. / Coronavirus (CoV) Nsp2 C-terminal domain profile. / Nonstructural protein 2, N-terminal domain, coronavirus / Non-structural protein 2, C-terminal domain, coronavirus / NSP3, second ubiquitin-like (Ubl) domain, coronavirus / Coronavirus Nsp3a Ubl domain profile. / Coronavirus Nsp3d Ubl domain profile. / NSP3, first ubiquitin-like (Ubl) domain, coronavirus / Peptidase family C16 domain profile. / : / :
Similarity search - Domain/homology
PHQ-Leu-Leu-Leu-aldehyde MG-132, bound form / Chem-ALD / Replicase polyprotein 1ab
Similarity search - Component
Biological speciesSevere acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å
AuthorsCostanzi, E. / Demitri, N. / Giabbai, B. / Storici, P.
Funding supportEuropean Union, 1items
OrganizationGrant numberCountry
European Commission101003551European Union
CitationJournal: Int J Mol Sci / Year: 2021
Title: Structural and Biochemical Analysis of the Dual Inhibition of MG-132 against SARS-CoV-2 Main Protease (Mpro/3CLpro) and Human Cathepsin-L.
Authors: Costanzi, E. / Kuzikov, M. / Esposito, F. / Albani, S. / Demitri, N. / Giabbai, B. / Camasta, M. / Tramontano, E. / Rossetti, G. / Zaliani, A. / Storici, P.
History
DepositionFeb 8, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Apr 7, 2021Provider: repository / Type: Initial release
Revision 1.1Nov 24, 2021Group: Data collection / Database references ...Data collection / Database references / Source and taxonomy / Structure summary
Category: citation / citation_author ...citation / citation_author / database_2 / entity / entity_name_com / entity_src_gen / pdbx_database_proc / struct
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _entity.pdbx_description / _entity.pdbx_ec / _entity_name_com.name / _entity_src_gen.gene_src_common_name / _struct.pdbx_center_of_mass_x / _struct.pdbx_center_of_mass_y / _struct.pdbx_center_of_mass_z
Revision 1.2Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.3Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 3C-like proteinase
B: 3C-like proteinase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)68,6425
Polymers67,6512
Non-polymers9913
Water6,954386
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5140 Å2
ΔGint-31 kcal/mol
Surface area25110 Å2
MethodPISA
Unit cell
Length a, b, c (Å)47.282, 54.918, 59.503
Angle α, β, γ (deg.)114.649, 99.955, 90.536
Int Tables number1
Space group name H-MP1
Space group name HallP1
Symmetry operation#1: x,y,z

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Components

#1: Protein 3C-like proteinase / 3CL-PRO / 3CLp / Main protease / Mpro / Non-structural protein 5 / nsp5 / SARS coronavirus main proteinase


Mass: 33825.547 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: P0DTD1, SARS coronavirus main proteinase
#2: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#3: Chemical ChemComp-ALD / N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide


Type: Peptide-like / Class: Inhibitor / Mass: 477.637 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C26H43N3O5 / Feature type: SUBJECT OF INVESTIGATION / References: PHQ-Leu-Leu-Leu-aldehyde MG-132, bound form
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 386 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.04 Å3/Da / Density % sol: 39.59 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop
Details: 0.05M Magnesium chloride hexahydrate , 0.1M MES pH 6.5, 5 % w/vPEG 4000, 10% v/v 2-Propanol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ELETTRA / Beamline: 11.2C / Wavelength: 0.9999 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 3, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9999 Å / Relative weight: 1
ReflectionResolution: 1.8→53.05 Å / Num. obs: 47488 / % possible obs: 96.6 % / Redundancy: 3.4 % / Biso Wilson estimate: 23.18 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.071 / Net I/σ(I): 10.4
Reflection shellResolution: 1.8→1.84 Å / Num. unique obs: 2756 / CC1/2: 0.566

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Processing

Software
NameVersionClassification
PHENIX1.19.1_4122refinement
PHENIX1.19.1_4122refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7BBE

7bbe
PDB Unreleased entry


Resolution: 1.8→49.71 Å / SU ML: 0.2046 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 22.6733
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2147 2412 5.08 %
Rwork0.1787 45062 -
obs0.1805 47474 96.62 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 31.37 Å2
Refinement stepCycle: LAST / Resolution: 1.8→49.71 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4701 0 69 386 5156
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01064878
X-RAY DIFFRACTIONf_angle_d0.94376627
X-RAY DIFFRACTIONf_chiral_restr0.0582749
X-RAY DIFFRACTIONf_plane_restr0.0076860
X-RAY DIFFRACTIONf_dihedral_angle_d14.05691757
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.8-1.840.29611390.25942592X-RAY DIFFRACTION95.49
1.84-1.880.31611180.26082668X-RAY DIFFRACTION95.71
1.88-1.920.2861360.2452628X-RAY DIFFRACTION95.41
1.92-1.970.27281430.22542609X-RAY DIFFRACTION94.47
1.97-2.030.23271450.20672600X-RAY DIFFRACTION96.21
2.03-2.090.22571310.1992652X-RAY DIFFRACTION96.46
2.09-2.150.29231320.19142687X-RAY DIFFRACTION96.54
2.15-2.230.2251540.18482641X-RAY DIFFRACTION97.05
2.23-2.320.20881370.19142632X-RAY DIFFRACTION96.68
2.32-2.420.24551510.18162665X-RAY DIFFRACTION97.61
2.42-2.550.23741680.18432685X-RAY DIFFRACTION97.41
2.55-2.710.221380.18862674X-RAY DIFFRACTION97.37
2.71-2.920.21951250.18422685X-RAY DIFFRACTION97.27
2.92-3.220.19621680.17872662X-RAY DIFFRACTION97.96
3.22-3.680.20621480.15732660X-RAY DIFFRACTION97.2
3.68-4.640.17021390.14122673X-RAY DIFFRACTION97.2
4.64-49.710.18881400.1692649X-RAY DIFFRACTION96.57
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.73808796983-0.7782230821450.7974856887261.51981225886-0.5325937031231.75645210588-0.2360310939380.001823749518950.1623513857220.1115771917140.134643710648-0.0634442970707-0.349024125959-0.1171564233650.08985809672770.224830904679-0.0117007062973-0.007405161469720.1796234117760.0008914458172120.174117717969-9.977965480894.7244406052-24.3452374011
22.52956448752-0.2449227049640.7189698444093.311411238861.136452233.77541849255-0.163827481809-0.02632988306960.2516835333760.149788897018-0.03038088972680.318384736384-0.277580331831-0.3733071332830.1746916008550.2736109550250.0408398278948-0.02444541966160.3125494961910.0337534394730.277003449859-22.56867138899.22334087027-33.3674906652
30.968989375214-0.666742466915-0.2033864210934.397295663761.118158135981.00550037364-0.02677208857780.09083596549260.296057977191-0.0107980368803-0.108503816746-0.321261589881-0.4360339432090.1637747520710.0779118026930.332725102918-0.0601364104733-0.05175564241890.1795061896660.07397177523480.29639398661-2.453062761756.89489910242-30.3345941115
42.01922948991-1.006335050870.3307371665761.924036791290.2607806026092.86159398697-0.09595199302350.09657972767830.0202811590563-0.05259145216310.0756317704626-0.04519828755110.03347776707150.04483479110490.0264440977330.15881384255-0.01180699420350.008976703002920.197751207920.0007248576488370.170208587185-8.05952782665-3.87114862031-27.2616813074
51.92643111547-0.9735536815290.01668000887080.957369840072-0.1271418796160.2796610004450.040542449443-0.0114076385918-0.141433428668-0.03333223555570.001319884936090.1072998501070.0483223971664-0.0105431083741-0.06185723387310.1516618011720.001369387743540.01437803714440.188509413666-0.006803497934970.173451166582-2.25716071138-16.3071925171-29.6361852898
62.5356881995-0.211566219468-0.1639429036351.957722940460.5473119978722.76209561451-0.07435188863730.0342194779816-0.1608840740550.05802547407710.0908214871736-0.08273770822190.2055805722110.233495600124-0.02013875502710.1653878824850.009892459381270.01242938931470.225641869727-0.02135671765450.18813457086810.9109801463-22.7681119216-26.8083018837
72.062585591010.870247329531.453809410961.076191137580.3522974231162.20749705532-0.3116618543470.1280436622750.27149699417-0.1136995010170.09351130028780.0523485926654-0.4342961337550.1553548695110.2083601344330.2439346700250.00366806646926-0.0182092788180.197497769225-0.02577012346930.2291077657217.717915213744.86303846366-6.36588049498
82.607432209241.38847066670.7297546541943.32697498140.0993129705742.39525581103-0.141537346272-0.2378673449630.2798025508460.00490666684666-0.100897340512-0.318370267261-0.5240364674010.2634891496570.2269607670960.333805729836-0.00545638733456-0.05556416009180.309135382794-0.0628423440680.28597660481314.52331445038.840963479451.87938195169
91.716525546570.929104870580.4163496963862.31667185755-0.5581516772112.8019660528-0.0790604395583-0.1496336466630.156970676284-0.04506447234290.03215957109820.0258960359668-0.0819812751691-0.1099113585920.0441772497290.1383427418940.007994975545360.004392540417570.20891192059-0.04031799930490.1887182294936.24411391106-4.03353399205-3.43548368405
102.112439959880.795241955688-0.1932768465720.7901701833350.06410504936440.134863832546-0.05281569443480.04223165655630.0179932456365-0.003690802668920.0310522331633-0.0985716327442-0.04916544611610.0616431266909-2.70263695105E-50.199522655047-0.01104563467450.02217538115350.2450164942380.01934973170490.21517522461-0.0817671209744-16.28948118-1.96082287871
111.782595045990.570704066534-0.6649880751892.75745212839-1.371200768245.39937276169-0.196475281709-0.0746956546785-0.301430280170.06704944684380.2439801641590.1210299710410.42207977491-0.227381277895-0.04611547744830.2936534051340.01901005408470.0501130855310.230700091017-0.001766871927040.260229246602-13.9042399084-29.2422888162-2.17184010141
121.719132791760.0810849991712-0.2371413625221.6049151794-0.7336963263982.16035872127-0.1079517874970.081771317697-0.091314686875-0.02155888510680.08304098768360.01980206868160.0942412781976-0.06875650760050.03384968295020.1686509217378.30395969229E-50.01717813060170.2559239791850.006276368602450.177509994824-12.2390741462-19.1855898531-5.2076339284
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 43 )AA1 - 431 - 43
22chain 'A' and (resid 44 through 91 )AA44 - 9144 - 91
33chain 'A' and (resid 92 through 110 )AA92 - 11092 - 110
44chain 'A' and (resid 111 through 185 )AA111 - 185111 - 185
55chain 'A' and (resid 186 through 213 )AA186 - 213186 - 213
66chain 'A' and (resid 214 through 302 )AA214 - 302214 - 302
77chain 'B' and (resid 1 through 43 )BB1 - 431 - 43
88chain 'B' and (resid 44 through 110 )BB44 - 11044 - 110
99chain 'B' and (resid 111 through 185 )BB111 - 185111 - 185
1010chain 'B' and (resid 186 through 214 )BB186 - 214186 - 214
1111chain 'B' and (resid 215 through 237 )BB215 - 237215 - 237
1212chain 'B' and (resid 238 through 305 )BB238 - 305238 - 305

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Jul 12, 2017. Major update of PDB

Major update of PDB

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External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

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Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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