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- PDB-7nc7: Crystal structure of fructose-bisphosphate aldolases FBAC from Ba... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7nc7 | ||||||
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Title | Crystal structure of fructose-bisphosphate aldolases FBAC from Bacillus methanolicus | ||||||
![]() | Fructose-bisphosphate aldolase | ||||||
![]() | LYASE / glycolytic aldolase | ||||||
Function / homology | ![]() fructose-bisphosphate aldolase / fructose-bisphosphate aldolase activity / fructose 1,6-bisphosphate metabolic process / glycolytic process / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Einsle, O. / Zhang, L. / Guetle, D. / Jacquot, J.P. | ||||||
![]() | ![]() Title: Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference Authors: Schultenkamper, K. / Gutle, D.D. / Lopez, M.G. / Keller, L.B. / Zhang, L. / Einsle, O. / Jacquot, J.P. / Wendisch, V.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 117.8 KB | Display | ![]() |
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PDB format | ![]() | 91.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 910.4 KB | Display | ![]() |
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Full document | ![]() | 917.8 KB | Display | |
Data in XML | ![]() | 23.1 KB | Display | |
Data in CIF | ![]() | 32.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7nccC ![]() 3q94S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30742.129 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: MGA3 / ATCC 53907 / Gene: fbaC, BMMGA3_16125 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 38.62 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.1 M sodium acetate trihydrate pH 4.5, 2.0 M ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jul 31, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→27.42 Å / Num. obs: 24421 / % possible obs: 99.8 % / Redundancy: 4.1 % / CC1/2: 0.996 / Net I/σ(I): 6.7 |
Reflection shell | Resolution: 2.2→2.28 Å / Num. unique obs: 2158 / CC1/2: 0.521 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3Q94 Resolution: 2.2→27.42 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.913 / SU B: 9.398 / SU ML: 0.227 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.319 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 155.81 Å2 / Biso mean: 36.602 Å2 / Biso min: 17.22 Å2
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Refinement step | Cycle: final / Resolution: 2.2→27.42 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.257 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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