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- PDB-7n9v: CA-targeting nanobody is a tool for studying HIV-1 capsid lattice... -

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IDまたはキーワード:

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基本情報

登録情報
データベース: PDB / ID: 7n9v
タイトルCA-targeting nanobody is a tool for studying HIV-1 capsid lattice interactions
要素
  • Capsid protein
  • Nanobody-GYAR
キーワードVIRAL PROTEIN/IMMUNE SYSTEM / HIV-1 / hexamer / nanobody / CypA / VIRAL PROTEIN-IMMUNE SYSTEM complex
機能・相同性
機能・相同性情報


viral nucleocapsid / host cell cytoplasm / viral translational frameshifting / host cell nucleus / structural molecule activity / virion membrane / RNA binding / zinc ion binding / ATP binding / cytoplasm
類似検索 - 分子機能
: / gag protein p24 N-terminal domain / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / Retroviral matrix protein / Retrovirus capsid, C-terminal / Retrovirus capsid, N-terminal ...: / gag protein p24 N-terminal domain / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / Retroviral matrix protein / Retrovirus capsid, C-terminal / Retrovirus capsid, N-terminal / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile.
類似検索 - ドメイン・相同性
生物種Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
Lama glama (ラマ)
手法X線回折 / シンクロトロン / 分子置換 / 解像度: 3.45 Å
データ登録者Tripler, T.N.
資金援助 米国, 2件
組織認可番号
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)P50GM082251 米国
National Institutes of Health/National Cancer Institute (NIH/NCI)F32 GM131426 01A1 米国
引用ジャーナル: To Be Published
タイトル: CA-targeting nanobody is a tool for studying HIV-1 capsid lattice interactions
著者: Gerber, E.E. / Digiananttonio, K.M. / Tripler, T.N. / Smaga, S.S. / Summers, B.J. / Xiong, Y.
履歴
登録2021年6月18日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02022年6月22日Provider: repository / タイプ: Initial release
改定 1.12023年10月18日Group: Data collection / Refinement description
カテゴリ: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

ダウンロードとリンク

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集合体

登録構造単位
C: Capsid protein
A: Capsid protein
D: Capsid protein
G: Capsid protein
I: Capsid protein
K: Capsid protein
J: Nanobody-GYAR
B: Nanobody-GYAR
E: Nanobody-GYAR
F: Nanobody-GYAR
H: Nanobody-GYAR
L: Nanobody-GYAR


分子量 (理論値)分子数
合計 (水以外)220,64912
ポリマ-220,64912
非ポリマー00
00
1


  • 登録構造と同一
  • 登録者が定義した集合体
  • 根拠: gel filtration
タイプ名称対称操作
identity operation1_555x,y,z1
単位格子
Length a, b, c (Å)102.548, 140.378, 125.186
Angle α, β, γ (deg.)90.000, 113.896, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
非結晶学的対称性 (NCS)NCSドメイン:
IDEns-ID詳細
d_1ens_1(chain "A" and (resid 1 through 177 or resid 183 through 221))
d_2ens_1(chain "C" and (resid 1 through 88 or resid 93 through 177 or resid 183 through 221))
d_3ens_1(chain "D" and (resid 1 through 88 or resid 93 through 177 or resid 183 through 221))
d_4ens_1(chain "G" and (resid 1 through 88 or resid 93 through 177 or resid 183 through 221))
d_5ens_1(chain "I" and (resid 1 through 88 or resid 93 through 177 or resid 183 through 221))
d_6ens_1(chain "K" and (resid 1 through 88 or resid 93 through 221))
d_1ens_2chain "B"
d_2ens_2chain "E"
d_3ens_2chain "F"
d_4ens_2chain "H"
d_5ens_2chain "J"
d_6ens_2chain "L"

NCSドメイン領域:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1PROALAB1 - 173
d_12ens_1ASNVALB179 - 217
d_21ens_1PROALAA1 - 88
d_22ens_1PROALAA90 - 174
d_23ens_1ASNVALA178 - 216
d_31ens_1PROALAC1 - 88
d_32ens_1PROALAC90 - 174
d_33ens_1ASNVALC180 - 218
d_41ens_1PROALAD1 - 88
d_42ens_1PROALAD93 - 177
d_43ens_1ASNVALD183 - 221
d_51ens_1PROALAE1 - 88
d_52ens_1PROALAE93 - 177
d_53ens_1ASNVALE183 - 221
d_61ens_1PROALAF1 - 88
d_62ens_1PROVALF93 - 216
d_11ens_2ASPSERH1 - 113
d_21ens_2ASPSERI1 - 113
d_31ens_2ASPSERJ1 - 113
d_41ens_2ASPSERK1 - 113
d_51ens_2ASPSERG1 - 113
d_61ens_2ASPSERL1 - 113

NCSアンサンブル:
ID
ens_1
ens_2

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要素

#1: タンパク質
Capsid protein


分子量: 24639.279 Da / 分子数: 6 / 断片: UNP residues 133-354 / 由来タイプ: 組換発現
由来: (組換発現) Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
遺伝子: gag / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: B6DRA0
#2: 抗体
Nanobody-GYAR


分子量: 12135.625 Da / 分子数: 6 / 由来タイプ: 組換発現 / 由来: (組換発現) Lama glama (ラマ) / 発現宿主: Escherichia coli BL21(DE3) (大腸菌)

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実験情報

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実験

実験手法: X線回折 / 使用した結晶の数: 1

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試料調製

結晶マシュー密度: 3.77 Å3/Da / 溶媒含有率: 67.37 %
結晶化温度: 297 K / 手法: マイクロバッチ法 / 詳細: potassium nitrate, pH 6.9, 20% PEG3350

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データ収集

回折平均測定温度: 100 K / Serial crystal experiment: N
放射光源由来: シンクロトロン / サイト: APS / ビームライン: 24-ID-C / 波長: 0.9791 Å
検出器タイプ: DECTRIS PILATUS 6M-F / 検出器: PIXEL / 日付: 2018年10月29日
放射プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長波長: 0.9791 Å / 相対比: 1
反射解像度: 3.45→50 Å / Num. obs: 39258 / % possible obs: 96.2 % / 冗長度: 2.8 % / Biso Wilson estimate: 116.18 Å2 / Rmerge(I) obs: 0.11 / Rpim(I) all: 0.077 / Rrim(I) all: 0.134 / Χ2: 1.049 / Net I/σ(I): 6.6
反射 シェル

Diffraction-ID: 1

解像度 (Å)冗長度 (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) allΧ2% possible all
3.5-3.562.80.82219940.5480.5711.0050.87597.3
3.56-3.632.80.76519590.5690.5350.9370.92397.2
3.63-3.692.80.71619690.6180.5040.8790.96397.1
3.69-3.772.70.5619670.7110.3980.690.97896.9
3.77-3.852.80.49519660.7760.3480.6081.00397.2
3.85-3.942.70.4319340.8030.3070.5311.06395.6
3.94-4.042.60.31719100.8880.2310.3941.03194.3
4.04-4.152.50.30218920.8990.2250.3781.09292.7
4.15-4.272.80.25619620.9190.1810.315197.1
4.27-4.412.90.21219650.9480.1460.2581.00496.8
4.41-4.572.90.17519520.9620.1210.2141.10395.7
4.57-4.752.80.16119450.9640.1110.1971.18296
4.75-4.972.80.13419840.9720.0930.1641.16596.5
4.97-5.232.70.13719520.9690.0980.1691.14996.3
5.23-5.552.60.1319070.9740.0940.1621.293.2
5.55-5.9830.11419990.9850.0770.1381.13698.5
5.98-6.5830.0920010.9880.0610.111.08197.9
6.58-7.532.80.06920150.990.0470.0841.08897.4
7.53-9.482.70.05719810.9940.0390.0691.01696
9.48-502.80.0520040.9940.0350.0620.93195.4

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解析

ソフトウェア
名称バージョン分類
PHENIX1.19.1_4122精密化
DENZOデータ削減
HKL-2000データスケーリング
PDB_EXTRACT3.27データ抽出
PHASER位相決定
精密化構造決定の手法: 分子置換
開始モデル: PDB entries 5O2U & 3H47
解像度: 3.45→46.88 Å / SU ML: 0.5138 / 交差検証法: FREE R-VALUE / σ(F): 1.35 / 位相誤差: 29.8309
立体化学のターゲット値: GeoStd + Monomer Library + CDL v1.2
Rfactor反射数%反射Selection details
Rfree0.2575 1997 5.09 %RANDOM
Rwork0.2123 37218 --
obs0.2147 39215 91.94 %-
溶媒の処理減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL
原子変位パラメータBiso mean: 168.99 Å2
精密化ステップサイクル: LAST / 解像度: 3.45→46.88 Å
タンパク質核酸リガンド溶媒全体
原子数15089 0 0 0 15089
拘束条件
Refine-IDタイプDev ideal
X-RAY DIFFRACTIONf_bond_d0.007115390
X-RAY DIFFRACTIONf_angle_d1.399620891
X-RAY DIFFRACTIONf_chiral_restr0.07662339
X-RAY DIFFRACTIONf_plane_restr0.00852729
X-RAY DIFFRACTIONf_dihedral_angle_d4.62462121
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDタイプRms dev position (Å)
ens_1d_2BX-RAY DIFFRACTIONTorsion NCS1.28529102178
ens_1d_3BX-RAY DIFFRACTIONTorsion NCS0.778678223006
ens_1d_4BX-RAY DIFFRACTIONTorsion NCS1.41720889347
ens_1d_5BX-RAY DIFFRACTIONTorsion NCS1.3642064415
ens_1d_6BX-RAY DIFFRACTIONTorsion NCS1.15852736795
ens_2d_2HX-RAY DIFFRACTIONTorsion NCS0.543810989566
ens_2d_3HX-RAY DIFFRACTIONTorsion NCS0.50625235437
ens_2d_4HX-RAY DIFFRACTIONTorsion NCS0.408384225718
ens_2d_5HX-RAY DIFFRACTIONTorsion NCS0.558337638122
ens_2d_6HX-RAY DIFFRACTIONTorsion NCS0.591552935454
LS精密化 シェル
解像度 (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.45-3.540.4736640.35971106X-RAY DIFFRACTION38.37
3.54-3.640.35511620.31882733X-RAY DIFFRACTION96.08
3.64-3.740.32521440.3042783X-RAY DIFFRACTION96.98
3.74-3.860.32541530.27232781X-RAY DIFFRACTION97.02
3.86-40.35121350.2732786X-RAY DIFFRACTION95.36
4-4.160.28181690.25192656X-RAY DIFFRACTION93.08
4.16-4.350.28651380.21392809X-RAY DIFFRACTION96.91
4.35-4.580.25021490.20272765X-RAY DIFFRACTION96.14
4.58-4.870.22481370.19042780X-RAY DIFFRACTION96.18
4.87-5.240.28121440.20282791X-RAY DIFFRACTION96.1
5.24-5.770.29291670.22592725X-RAY DIFFRACTION94.98
5.77-6.60.23051320.21852869X-RAY DIFFRACTION98.07
6.6-8.310.25221550.19532796X-RAY DIFFRACTION96.16
8.31-46.880.19041480.16032838X-RAY DIFFRACTION95.8
精密化 TLS

手法: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
14.544642957020.845665187046-0.07484059333464.1290955026-0.6990775165264.83810355250.022687539464-0.110318160936-0.1961762890460.411489527379-0.04332205290670.2616190960070.166439324838-0.115398949142-0.0202482112860.8180623078790.03978815784860.1334891717850.64749547342-0.06254446483460.899629359682-71.00489912-35.032433206946.4318625056
29.747289456282.66845094779-0.904527121354.93571951293-0.7533868409983.242529806340.720790003291-1.10395872141-0.6071339670691.29925653833-0.295540506893-0.565255023053-0.09941140240520.33912341822-0.01594218937181.02621157304-0.0611745680785-0.05564004653460.5367967818910.04757008535660.80226888352-61.4477794161-38.823394320249.9059616614
33.67914082791-2.463710796241.441646140439.164478461291.793792468525.42017453651-0.2105203818220.02074399754770.4528105055472.269738604430.851314457703-2.164397225810.5963501061711.1693471274-0.3152048977971.652018961710.495789162498-0.7637048039091.41870704073-0.1001473498381.98861852082-37.5749240012-30.969304462563.392835011
44.15955392772-0.678283189251.668642772554.717184514162.716129712756.93192312543-0.0376141468510.0837602876894-0.048790501716-1.06341613451-0.3159773461670.99859919042-0.478946821548-0.6613124995430.2258693750381.346413639870.0296453741156-0.2544043125080.8093001382070.1023948581061.02006762833-81.3177152983-0.95510498431122.4754984878
53.55094528979-0.826164441381-1.410969134588.483070305132.491429414314.311484180440.2297143486550.660027324888-0.458877003613-1.2280435106-0.3628651847410.34565323314-0.0494267838899-0.1756335926020.1980149193631.86859150831-0.0314060550656-0.0389401236711.44055501338-0.2587063341320.890305626554-65.2995849885-27.66355150363.81112363656
65.367796192210.333403341518-0.5037261041953.57444895661.934973897824.47862185156-0.132868901364-0.872586685727-0.4233853095151.281885496250.217751215970.4044134465150.027910500136-0.236400279309-0.1170280862950.9884410526330.189120982050.1409944880750.7727395201260.1446215714650.920553322648-63.1936850503-20.148873980564.6907951428
72.77089711134.64917336039-0.4595347874746.852953421530.6966783867952.05828703041-0.2737638016750.429870994986-0.6761201896491.134371069050.633551241402-1.563589307760.1155066762440.674661662223-0.1968526680341.045461009850.0253492731982-0.2925816499891.1332915109-0.05055305190631.3199421153-43.3173207881-5.6019186119664.6702530905
84.637179888461.043193561792.582968373939.171663677962.083909452932.04887737675-0.676395472034-0.1316090850260.4301684099871.1212069154-0.4246060534470.0042361098043-0.360903747883-0.0654625465613-0.09473428954371.34903048403-0.440179798231-0.1314298518691.70751099382-0.3113445357181.47517082588-37.190168362711.588162229366.7129038039
94.571313881240.327254512534-0.8687050285174.648300395640.4299207323785.42686293792-0.246176667918-0.4153546364950.2300485665680.5341414888230.1087003779310.258466156853-0.623449756896-0.1475473074050.02708716856951.100126595440.000294661708173-0.04093871640790.760224566430.0449047947460.814559339482-63.57277460055.3666936073662.4589772116
102.125247427690.4712504621760.07002378530475.76425867157-2.965777400177.11416055153-0.000281896055908-0.4656321715450.320009874382-0.132777610115-0.1294994627-0.704241115208-0.5253012577380.0270063738045-0.1235794680361.67553669903-0.2694547097750.02130518946161.118734953970.07801519962691.11448991665-48.465101952826.189047048635.7038375284
113.862254530530.3202432719580.01000439644835.05272842916-0.9833344682132.005736654290.1804083198890.134936170147-0.112650354844-0.328108879727-0.09098524890240.8698459734860.0299687962409-0.487766481535-0.06715721271320.92023063176-0.00396762578574-0.05821742392330.968756273845-0.1061213903340.954722145737-81.353455513-24.841320541226.0538593432
124.61317867822-2.600353171673.210362114072.67808085705-2.221240300652.808840650711.014008214670.7567978929620.244619000575-1.26859461246-0.523193492246-0.2731894280131.013385265370.78416785584-0.09390203758071.206973941390.166944714018-0.05087885775560.876884600811-0.1541304126321.14362143496-60.5454284388-38.663825287726.2062125115
135.80042977753-2.175490009943.743727436375.20818849709-2.741433666198.639284174960.7159579308790.567008155643-0.637384984983-0.113451529886-0.2028883547490.82137442981.832295749150.249657097096-0.4012089263140.9660595535360.1645948985840.1496202022980.847721477186-0.06219277607061.39433586095-49.8922860998-50.828707969435.1715029657
141.497881125421.736044217010.1878508661844.909352389030.1883597934865.066460738880.40341628831-0.230104302501-2.415380480120.661258774191-1.129172552610.5855603294361.26518957452-1.231261574820.310101835641.06151709322-0.0689631457237-0.06862800150931.10154557251-0.008116984276571.46456997304-81.31169619422.4779849919547.4915456466
153.373992857341.17046952464-1.727754422373.16423176444-0.6998448700426.852082668510.7355043633260.251631653398-0.136538268341-1.00150057062-0.6791205063780.306549963712-0.2173429722280.9880171787840.2553806245471.14648966353-0.0493950235123-0.01486618710250.7531161649240.08547063398430.75255736715-65.48943773165.9916871898534.6078888433
167.58723428017-1.26487788873-0.7845459880643.990241913890.8086020121823.91194273852-0.541517753871-1.31347574619-0.580188013061-0.6139515278470.03952859016550.5945786338590.695545826821.479323522110.1390659832761.3821921647-0.2136980800850.12886918860.69081881513-0.03577217205680.834285126426-65.013691473412.530766411342.2157378104
172.44118241395-2.30308334303-2.697465049253.775544929041.978890747323.923488550860.3962556448391.174160230131.59058026663-0.844394399902-0.334950749456-0.38711365495-2.509558247790.1783076284410.02793194459792.13311585201-0.185119158027-0.1155475272120.8110506797840.1614677222021.16073265151-68.916432951125.809536441336.9405586419
183.58893973570.758085665567-0.5403372596932.98644936129-1.833004679135.07904686658-0.358150168010.2517873523851.08668627445-0.1848659478950.4039444197421.21135787119-1.78103338119-0.6295175537240.0725540075831.435970793990.04589217958850.0612207766230.81314434071-0.09209283722491.41082040145-83.09797528921.525782651648.0733234284
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676.104841558850.7646105452676.061673130919.781622441371.651908418536.05991092701-0.6061137396361.82060201505-0.532084264890.01566262267960.311576434835-1.42348665192-0.9704096945270.233958306293-0.2922916312275.715016864380.1666522574430.5336223897322.835504943750.1350452310891.85878349652-67.0891179759-1.20967143084-21.0083814481
687.6574814542-6.352500313831.952083604039.101643515580.007789230168431.170279878340.1195214265882.483605707780.143832474316-2.101247847251.361099575310.842160303935-0.7328787158090.08928310766270.5880108985684.9707349381-0.878291473240.07726772547215.11150679902-0.4683389356952.74235975702-88.53820857066.06620815889-27.6280682307
692.018237088920.972010189439-0.519737678065.227204905431.719271212490.673841356557-0.9985507037343.97467773368-2.53598372613-0.604929464174-0.009143472989260.46281284989-2.861902037430.458755883125-0.260016691185.525716528580.811466662086-0.0722884092533.63308434324-0.651665597631.18142166275-73.6123851046-0.677896777287-15.6402465059
709.068954468232.01009509539-2.021852278246.340412564290.5043249647770.5974147919211.80757171650.3646411841710.938732360233-0.09116239644181.002424804490.261100876249-1.04290349074-1.488740468660.6780265299594.855978577230.622542165665-0.5363897855572.154071652850.07176396657470.90706113632-76.603322356111.6669032504-13.6389109721
718.47128203156-3.55021046976-1.408858945474.147483566371.962692369510.865150040223-0.899893500893-0.5666803510270.9399894794660.3649038246990.2573195179052.249384528510.184678646531-1.539156956930.3071865637693.950421255440.044722576968-0.7313553223262.12156785809-0.09280649969672.93395531182-83.77882807983.78136790945-8.12234398521
723.855942872881.032468024844.34341484611.63656483029-0.01333146104415.92589308204-0.4610010241951.20102260554-1.27323353607-0.03629758137640.278623286222-0.908008415148-0.8981945051840.960651409024-0.5300504252586.57105814658-0.5065975888051.159243997692.1535125659-0.5036636187183.46821318945-77.9038280268-8.54937496373-15.0343136106
736.15689739737-1.196412470180.7313910035423.668926277750.1811135240944.37298274903-0.8285369147671.88968137144-0.102103978261-2.32540750379-1.04298460542.5580849402-1.25492328614-1.60919980427-0.3151703495975.530522021530.635148479021-2.151528057054.18364853956-2.175385201243.00214561044-81.4879557458-1.48106569362-17.9044570067
743.343010946580.298687633455-4.274914624671.341745743620.5221646166786.11221075958-0.3555312358190.3803279304662.17067350665-1.723249041780.00808802178356-0.447237043048-1.213536002690.2281750822351.03316940723.636844970480.816559589267-0.4498487844552.48568116350.6517835179752.93944959757-89.514340107510.0541587638-20.6486458065
752.597295301871.62685856288-2.153890320481.92844237473-1.14107431111.84095893207-0.3961655782533.19486793944-0.215395646015-1.959061132141.654362031770.253785246766-0.964569538613-0.6114042075671.6380249655.404063398331.42519266504-1.112840548623.62777779323-0.581071228320.577900939425-73.70047994676.46044371048-17.9753322004
765.410227051832.86956620696-1.534591863777.196377470474.482690137396.05362348183-0.44311967391.85798338128-1.853013056030.0285892151521.91285141428-2.374554459630.4513384526691.56509430003-0.5242465967473.353956881670.9812277493690.6178712268612.46425590064-0.127210546881.90128668914-63.44365200622.49058473401-12.7141897822
770.855793401697-1.241518283950.342808426644.471224295920.4106019052060.801251006534-0.5640012508872.964409425711.18188600667-2.501887312750.1519890024772.02344844089-0.9509068880231.219166649220.2296836020985.021475320770.829129317863-0.809539533854.870077035-0.07713308329322.10674790095-78.73805111338.49919264096-26.3817479207
785.829226664145.177406610843.420011925316.534305951260.2452661432025.90882471063-3.475264473541.65521328059-3.83369593938-0.4069991286950.0960323905213-0.6390354129821.4217724104-1.630005811840.1385995529170.9146088465731.970080808431.792355073951.35052101362-1.882491355152.60706239955-57.7036212739-54.7048014327-2.58425829835
797.516631712665.550191721654.768403460274.151070636092.958010860827.4771478557-1.688930600612.03482848529-2.11656329427-1.157079666772.02509458451-1.149677090230.76079900158-1.033349601710.825395559715.48042786652-0.4181722166441.539769541893.045996889-1.682975147055.78935075862-78.2840371495-64.1937119165-9.18861635974
800.287410095064-0.572098964655-0.1707250471272.42796880544-0.3767413452140.300812618033-0.3716029163250.526852692706-3.21571453657-1.913436323360.5358186299570.02542237618962.4932174966-0.5082091545521.433864555013.092340679841.43844049539-0.5677810252121.42334522459-1.990539153823.60979352362-62.5603108694-55.04782871514.24993906383
818.815224767564.155375624157.979487104073.17631416842.744687024768.04229426406-2.493630164863.61124660863-4.51008519236-2.28259172583-0.5372899051330.1683088029272.07836633105-0.7480388494940.2508786361642.650858012340.2867519007550.3375293464662.86948449941-0.9354727726132.15045888436-69.2641441795-49.0757766102-4.02759840336
827.558623490292.78138942292.443685535585.045185760260.9304710925581.05465918037-1.727125369842.40687623973-1.93132947242-0.622049648459-0.06120292963360.1497650558510.63170631251-1.493891092160.8374538083942.298416458520.0497047917285-0.2094286998522.16084568133-0.9626697290632.2749328124-72.4448891656-46.1391682848-6.75989461461
833.927543209852.262025395563.5243822343.88431554080.4189582305064.66001052148-1.936924741110.461030799257-2.29377451591-0.7008014286070.3882871290950.07220226288250.7168518727321.321802651390.6186843785412.40757307923-0.4477680987930.1306032096441.95135759702-0.4515976855752.16714389921-77.5282076801-48.68935463473.56223849737
847.173639147481.21306897266-5.521271644622.1039634944-0.710760094064.27680492767-0.567321717593-0.651966543027-3.47077357093-0.829223774366-0.167306454846-0.820384110620.9201350948950.2392299405840.4666491890592.82700968360.1694165154081.039745867111.60334760574-0.6419633283293.90201263556-68.5030958044-58.04980619635.56774593535
852.171114397832.19081250329-3.088048358274.08857086098-2.592189009556.90529083114-1.736787921161.85406699924-1.48227256925-1.633189814710.95801047982-0.5525952439831.126346842830.4974444180140.6357522262634.74915556476-0.865021866669-1.772681312823.83933330113-0.8398936836363.71050005028-82.0884439417-56.987912927-8.16272785749
863.207030255590.326798534618-1.916491023764.6770807465-2.085380009911.94847424558-2.71527178437-0.00436137025562-3.20272882426-2.193994987180.420863204029-0.2833409444683.013132891530.3539493473350.07811709155992.16659219423-0.2094333592010.4542856590582.36600516692-1.162864851862.57348397271-62.6792759441-48.233287485-3.61797792065
870.7929256580880.557334840561.594444136056.39378097642.806127625494.52698953401-1.698951714722.26517848236-3.08060293375-1.961714945831.469387666970.397536536030.315766423013-1.301036851460.8297091497343.62652434770.20764836820.1337618353783.51056065947-1.589046345173.18320706072-70.4226104045-58.2427771218-11.7546076193
精密化 TLSグループ

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'C' and (resid 1 through 125 )CA1 - 1251 - 122
22chain 'C' and (resid 126 through 145 )CA126 - 145123 - 142
33chain 'C' and (resid 146 through 221 )CA146 - 221143 - 216
44chain 'A' and (resid 1 through 145 )AB1 - 1451 - 141
55chain 'A' and (resid 146 through 221 )AB146 - 221142 - 217
66chain 'D' and (resid 1 through 125 )DC1 - 1251 - 122
77chain 'D' and (resid 126 through 176 )DC126 - 176123 - 173
88chain 'D' and (resid 177 through 223 )DC177 - 223174 - 220
99chain 'G' and (resid 1 through 143 )GD1 - 1431 - 143
1010chain 'G' and (resid 144 through 223 )GD144 - 223144 - 223
1111chain 'I' and (resid 1 through 125 )IE1 - 1251 - 125
1212chain 'I' and (resid 126 through 175 )IE126 - 175126 - 175
1313chain 'I' and (resid 176 through 223 )IE176 - 223176 - 223
1414chain 'K' and (resid 1 through 16 )KF1 - 161 - 16
1515chain 'K' and (resid 17 through 48 )KF17 - 4817 - 48
1616chain 'K' and (resid 49 through 62 )KF49 - 6249 - 62
1717chain 'K' and (resid 63 through 83 )KF63 - 8363 - 83
1818chain 'K' and (resid 84 through 125 )KF84 - 12584 - 125
1919chain 'K' and (resid 126 through 145 )KF126 - 145126 - 145
2020chain 'K' and (resid 146 through 160 )KF146 - 160146 - 160
2121chain 'K' and (resid 161 through 176 )KF161 - 176161 - 176
2222chain 'K' and (resid 177 through 193 )KF177 - 193177 - 188
2323chain 'K' and (resid 194 through 205 )KF194 - 205189 - 200
2424chain 'K' and (resid 206 through 223 )KF206 - 223201 - 218
2525chain 'J' and (resid 1 through 7 )JG1 - 71 - 7
2626chain 'J' and (resid 8 through 17 )JG8 - 178 - 17
2727chain 'J' and (resid 18 through 32 )JG18 - 3218 - 32
2828chain 'J' and (resid 33 through 52 )JG33 - 5233 - 52
2929chain 'J' and (resid 53 through 67 )JG53 - 6753 - 67
3030chain 'J' and (resid 68 through 82 )JG68 - 8268 - 82
3131chain 'J' and (resid 82A through 95 )JG82 - 9583 - 98
3232chain 'J' and (resid 96 through 112 )JG96 - 11299 - 113
3333chain 'B' and (resid 1 through 7 )BH1 - 71 - 7
3434chain 'B' and (resid 8 through 17 )BH8 - 178 - 17
3535chain 'B' and (resid 18 through 32 )BH18 - 3218 - 32
3636chain 'B' and (resid 33 through 39 )BH33 - 3933 - 39
3737chain 'B' and (resid 40 through 52 )BH40 - 5240 - 52
3838chain 'B' and (resid 53 through 67 )BH53 - 6753 - 67
3939chain 'B' and (resid 68 through 76 )BH68 - 7668 - 76
4040chain 'B' and (resid 77 through 82 )BH77 - 8277 - 82
4141chain 'B' and (resid 82A through 87 )BH82 - 8783 - 90
4242chain 'B' and (resid 88 through 95 )BH88 - 9591 - 98
4343chain 'B' and (resid 96 through 103 )BH96 - 10399 - 104
4444chain 'B' and (resid 104 through 112 )BH104 - 112105 - 113
4545chain 'E' and (resid 1 through 17 )EI1 - 171 - 17
4646chain 'E' and (resid 18 through 24 )EI18 - 2418 - 24
4747chain 'E' and (resid 25 through 32 )EI25 - 3225 - 32
4848chain 'E' and (resid 33 through 39 )EI33 - 3933 - 39
4949chain 'E' and (resid 40 through 52 )EI40 - 5240 - 52
5050chain 'E' and (resid 53 through 67 )EI53 - 6753 - 67
5151chain 'E' and (resid 68 through 76 )EI68 - 7668 - 76
5252chain 'E' and (resid 77 through 82 )EI77 - 8277 - 82
5353chain 'E' and (resid 82A through 87 )EI82 - 8783 - 90
5454chain 'E' and (resid 88 through 95 )EI88 - 9591 - 98
5555chain 'E' and (resid 96 through 103 )EI96 - 10399 - 104
5656chain 'E' and (resid 104 through 112 )EI104 - 112105 - 113
5757chain 'F' and (resid 1 through 7 )FJ1 - 71 - 7
5858chain 'F' and (resid 8 through 17 )FJ8 - 178 - 17
5959chain 'F' and (resid 18 through 32 )FJ18 - 3218 - 32
6060chain 'F' and (resid 33 through 52 )FJ33 - 5233 - 52
6161chain 'F' and (resid 53 through 67 )FJ53 - 6753 - 67
6262chain 'F' and (resid 68 through 82 )FJ68 - 8268 - 82
6363chain 'F' and (resid 82A through 87 )FJ82 - 8783 - 90
6464chain 'F' and (resid 88 through 95 )FJ88 - 9591 - 98
6565chain 'F' and (resid 96 through 103 )FJ96 - 10399 - 104
6666chain 'F' and (resid 104 through 112 )FJ104 - 112105 - 113
6767chain 'H' and (resid 1 through 7 )HK1 - 71 - 7
6868chain 'H' and (resid 8 through 17 )HK8 - 178 - 17
6969chain 'H' and (resid 18 through 39 )HK18 - 3918 - 39
7070chain 'H' and (resid 40 through 52 )HK40 - 5240 - 52
7171chain 'H' and (resid 53 through 67 )HK53 - 6753 - 67
7272chain 'H' and (resid 68 through 76 )HK68 - 7668 - 76
7373chain 'H' and (resid 77 through 82 )HK77 - 8277 - 82
7474chain 'H' and (resid 82A through 87 )HK82 - 8783 - 90
7575chain 'H' and (resid 88 through 95 )HK88 - 9591 - 98
7676chain 'H' and (resid 96 through 103 )HK96 - 10399 - 104
7777chain 'H' and (resid 104 through 112 )HK104 - 112105 - 113
7878chain 'L' and (resid 1 through 7 )LL1 - 71 - 7
7979chain 'L' and (resid 8 through 17 )LL8 - 178 - 17
8080chain 'L' and (resid 18 through 32 )LL18 - 3218 - 32
8181chain 'L' and (resid 33 through 39 )LL33 - 3933 - 39
8282chain 'L' and (resid 40 through 52 )LL40 - 5240 - 52
8383chain 'L' and (resid 53 through 67 )LL53 - 6753 - 67
8484chain 'L' and (resid 68 through 82 )LL68 - 8268 - 82
8585chain 'L' and (resid 82A through 87 )LL82 - 8783 - 90
8686chain 'L' and (resid 88 through 103 )LL88 - 10391 - 104
8787chain 'L' and (resid 104 through 112 )LL104 - 112105 - 113

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万見について

-
お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月:コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか? / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例:em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る