+Open data
-Basic information
Entry | Database: PDB / ID: 7n69 | ||||||||||||||||||
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Title | Pre-fusion state 2 of EEEV with localized reconstruction | ||||||||||||||||||
Components |
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Keywords | VIRUS / EEEV / pre-fusion / localized reconstruction | ||||||||||||||||||
Function / homology | Function and homology information togavirin / T=4 icosahedral viral capsid / symbiont-mediated suppression of host gene expression / symbiont-mediated suppression of host toll-like receptor signaling pathway / host cell cytoplasm / symbiont entry into host cell / serine-type endopeptidase activity / fusion of virus membrane with host endosome membrane / host cell nucleus / virion attachment to host cell ...togavirin / T=4 icosahedral viral capsid / symbiont-mediated suppression of host gene expression / symbiont-mediated suppression of host toll-like receptor signaling pathway / host cell cytoplasm / symbiont entry into host cell / serine-type endopeptidase activity / fusion of virus membrane with host endosome membrane / host cell nucleus / virion attachment to host cell / host cell plasma membrane / virion membrane / structural molecule activity / proteolysis / RNA binding / membrane Similarity search - Function | ||||||||||||||||||
Biological species | Eastern equine encephalitis virus | ||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 14.1 Å | ||||||||||||||||||
Authors | Chen, C.-L. / Kuhn, R.J. / Klose, T. | ||||||||||||||||||
Funding support | United States, 5items
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Citation | Journal: Proc Natl Acad Sci U S A / Year: 2022 Title: Cryo-EM structures of alphavirus conformational intermediates in low pH-triggered prefusion states. Authors: Chun-Liang Chen / Thomas Klose / Chengqun Sun / Arthur S Kim / Geeta Buda / Michael G Rossmann / Michael S Diamond / William B Klimstra / Richard J Kuhn / Abstract: Alphaviruses can cause severe human arthritis and encephalitis. During virus infection, structural changes of viral glycoproteins in the acidified endosome trigger virus-host membrane fusion for ...Alphaviruses can cause severe human arthritis and encephalitis. During virus infection, structural changes of viral glycoproteins in the acidified endosome trigger virus-host membrane fusion for delivery of the capsid core and RNA genome into the cytosol to initiate virus translation and replication. However, mechanisms by which E1 and E2 glycoproteins rearrange in this process remain unknown. Here, we investigate prefusion cryoelectron microscopy (cryo-EM) structures of eastern equine encephalitis virus (EEEV) under acidic conditions. With models fitted into the low-pH cryo-EM maps, we suggest that E2 dissociates from E1, accompanied by a rotation (∼60°) of the E2-B domain (E2-B) to expose E1 fusion loops. Cryo-EM reconstructions of EEEV bound to a protective antibody at acidic and neutral pH suggest that stabilization of E2-B prevents dissociation of E2 from E1. These findings reveal conformational changes of the glycoprotein spikes in the acidified host endosome. Stabilization of E2-B may provide a strategy for antiviral agent development. | ||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7n69.cif.gz | 636.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7n69.ent.gz | 495.7 KB | Display | PDB format |
PDBx/mmJSON format | 7n69.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7n69_validation.pdf.gz | 865.7 KB | Display | wwPDB validaton report |
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Full document | 7n69_full_validation.pdf.gz | 907.9 KB | Display | |
Data in XML | 7n69_validation.xml.gz | 90.1 KB | Display | |
Data in CIF | 7n69_validation.cif.gz | 145.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n6/7n69 ftp://data.pdbj.org/pub/pdb/validation_reports/n6/7n69 | HTTPS FTP |
-Related structure data
Related structure data | 24201MC 7n6aC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 47938.141 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Eastern equine encephalitis virus (strain Florida 91-469) Strain: Florida 91-469 / Production host: Cricetinae gen. sp. (mammal) / References: UniProt: Q4QXJ7 #2: Protein | Mass: 47046.953 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Eastern equine encephalitis virus (strain Florida 91-469) Strain: Florida 91-469 / Production host: Cricetinae gen. sp. (mammal) / References: UniProt: Q4QXJ7 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Eastern equine encephalitis virus / Type: VIRUS / Entity ID: all / Source: RECOMBINANT | ||||||||||||||||||||||||||||||
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Molecular weight | Experimental value: NO | ||||||||||||||||||||||||||||||
Source (natural) | Organism: Eastern equine encephalitis virus | ||||||||||||||||||||||||||||||
Source (recombinant) | Organism: Cricetinae gen. sp. (mammal) / Cell: Baby hamster kidney cells | ||||||||||||||||||||||||||||||
Details of virus | Empty: NO / Enveloped: YES / Isolate: STRAIN / Type: VIRION | ||||||||||||||||||||||||||||||
Natural host | Organism: Equus caballus | ||||||||||||||||||||||||||||||
Virus shell | Diameter: 660 nm | ||||||||||||||||||||||||||||||
Buffer solution | pH: 5.5 / Details: pH 5.5 | ||||||||||||||||||||||||||||||
Buffer component |
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Specimen | Conc.: 0.2 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES Details: Estimate E2 protein concentration by SDS-PAGE with BSA ranging from 0.1 to 1 microgram. | ||||||||||||||||||||||||||||||
Specimen support | Details: 25 mA / Grid material: COPPER / Grid type: PELCO Ultrathin Carbon with Lacey Carbon | ||||||||||||||||||||||||||||||
Vitrification | Instrument: GATAN CRYOPLUNGE 3 / Cryogen name: ETHANE / Humidity: 80 % / Chamber temperature: 298 K / Details: blot for 3.3 seconds before plunging |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 81000 X / Cs: 2.7 mm / C2 aperture diameter: 100 µm |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Temperature (min): 100 K |
Image recording | Electron dose: 32 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 1 |
-Processing
EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 14.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 192060 / Algorithm: BACK PROJECTION Details: 192060 asymmetric units were used for reconstruction Symmetry type: POINT | ||||||||||||||||||||||||||||||||||||
Atomic model building | Protocol: FLEXIBLE FIT / Space: REAL | ||||||||||||||||||||||||||||||||||||
Atomic model building | PDB-ID: 6MX4 |