#256 - Apr 2021 SARS-CoV-2 Spike and Antibodies similarity (1)
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Assembly
Deposited unit
G: HIV-1 gp41 immunodominant region H: Fab 3D6 heavy chain F: HIV-1 gp41 immunodominant region E: HIV-1 gp41 immunodominant region L: Fab 3D6 light chain B: Fab 3D6 light chain A: Fab 3D6 heavy chain D: Fab 3D6 light chain C: Fab 3D6 heavy chain
Mass: 1590.798 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) HIV-1 06TG.HT008 (virus)
#2: Antibody
Fab3D6heavychain
Mass: 26649.836 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293T / Production host: Homo sapiens (human)
#3: Antibody
Fab3D6lightchain
Mass: 23270.859 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293T / Production host: Homo sapiens (human)
Mass: 18.015 Da / Num. of mol.: 1213 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.48 Å3/Da / Density % sol: 50.35 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.2 M magnesium chloride, 20% (w/v) PEG 8000, 0.1 M Tris-HCl, pH 8.5, and 0.59 mM n-undecyl-beta-D-maltoside
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N