+Open data
-Basic information
Entry | Database: PDB / ID: 7msl | ||||||
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Title | Structure of anti-CRISPR AcrIIC4 from Haemophilus parainfluenzae | ||||||
Components | AcrIIC4 | ||||||
Keywords | VIRAL PROTEIN / Anti-CRISPR proteins / AcrIIC4 / a Haemophilus parainfluenzae prophage | ||||||
Function / homology | IODIDE ION / Uncharacterized protein Function and homology information | ||||||
Biological species | Haemophilus parainfluenzae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SAD / Resolution: 1.73 Å | ||||||
Authors | Pan, C. / Maxwell, K.L. / Moraes, T.F. | ||||||
Funding support | Canada, 1items
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Citation | Journal: J.Mol.Biol. / Year: 2022 Title: Structural and Mechanistic Insight into CRISPR-Cas9 Inhibition by Anti-CRISPR Protein AcrIIC4 Hpa. Authors: Hwang, S. / Pan, C. / Garcia, B. / Davidson, A.R. / Moraes, T.F. / Maxwell, K.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7msl.cif.gz | 57.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7msl.ent.gz | 34.5 KB | Display | PDB format |
PDBx/mmJSON format | 7msl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7msl_validation.pdf.gz | 406.8 KB | Display | wwPDB validaton report |
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Full document | 7msl_full_validation.pdf.gz | 406.8 KB | Display | |
Data in XML | 7msl_validation.xml.gz | 5.6 KB | Display | |
Data in CIF | 7msl_validation.cif.gz | 6.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ms/7msl ftp://data.pdbj.org/pub/pdb/validation_reports/ms/7msl | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 10331.721 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Haemophilus parainfluenzae (bacteria) / Gene: NCTC10672_00033, NCTC10672_02354 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A377JKY9 | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.13 % |
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Crystal grow | Temperature: 294.15 K / Method: vapor diffusion, sitting drop / pH: 4.2 / Details: 35% PEG 600, 0.1M NaHPO4/citric acid pH 4.2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ROTATING ANODE / Type: Cu FINE FOCUS / Wavelength: 1.54 Å |
Detector | Type: RIGAKU / Detector: CCD / Date: Feb 2, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.73→36.87 Å / Num. obs: 10268 / % possible obs: 99.92 % / Redundancy: 2 % / Biso Wilson estimate: 23.55 Å2 / CC1/2: 0.998 / Net I/σ(I): 17.32 |
Reflection shell | Resolution: 1.73→1.8 Å / Num. unique obs: 990 / CC1/2: 0.67 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.73→36.87 Å / SU ML: 0.2102 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 29.2669 / Stereochemistry target values: GeoStd + Monomer Library
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 37.5 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.73→36.87 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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