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Open data
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Basic information
Entry | Database: PDB / ID: 7mhr | ||||||
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Title | KcsA E71V closed gate with K+ | ||||||
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![]() | MEMBRANE PROTEIN / ion channel | ||||||
Function / homology | delayed rectifier potassium channel activity / Potassium channel domain / Ion channel / voltage-gated potassium channel complex / identical protein binding / : / pH-gated potassium channel KcsA![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rohaim, A. / Li, J. / Weingarth, M. / Roux, B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A distinct mechanism of C-type inactivation in the Kv-like KcsA mutant E71V. Authors: Rohaim, A. / Vermeulen, B.J.A. / Li, J. / Kummerer, F. / Napoli, F. / Blachowicz, L. / Medeiros-Silva, J. / Roux, B. / Weingarth, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 220.3 KB | Display | ![]() |
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PDB format | ![]() | 174.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 23.5 KB | Display | |
Data in CIF | ![]() | 33.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7mhxC ![]() 7mjtC ![]() 7mk6C ![]() 7mubC ![]() 1k4cS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
#3: Protein | Mass: 13181.600 Da / Num. of mol.: 1 / Mutation: E71V, L90C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
#4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.86 Å3/Da / Density % sol: 68.1 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 50 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400 |
-Data collection
Diffraction | Mean temperature: 98 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 26, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.77→50.01 Å / Num. obs: 23491 / % possible obs: 99.7 % / Redundancy: 6.6 % / CC1/2: 0.977 / Net I/σ(I): 10.3 |
Reflection shell | Resolution: 2.77→2.92 Å / Num. unique obs: 3370 / CC1/2: 0.884 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1K4C Resolution: 2.77→50.01 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.933 / SU B: 18.29 / SU ML: 0.187 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.422 / ESU R Free: 0.25 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 195.85 Å2 / Biso mean: 59.316 Å2 / Biso min: 3.88 Å2
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Refinement step | Cycle: final / Resolution: 2.77→50.01 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.77→2.837 Å / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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