PDB-7lxr: Kinesin-like protein at 61F (Klp61f) bound to AMPPNP

ID or keywords:

Entry

Database: PDB / ID: 7lxr

Title

Kinesin-like protein at 61F (Klp61f) bound to AMPPNP

Components

Kinesin-like protein Klp61F

Keywords

CELL CYCLE / microtubule-binding ATP-binding cell division mitosis plus-end directed motor protein

Function / homology

Function and homology information

aster / plus-end directed microtubule sliding / fusome organization /

fusome / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins / centrosome separation / spindle elongation / mitotic spindle microtubule / positive regulation of Golgi to plasma membrane protein transport ...
Similarity search - Function

Biological species

Drosophila melanogaster (fruit fly)

Method

X-RAY DIFFRACTION /

MOLECULAR REPLACEMENT / Resolution: 1.744 Å

Authors

Worthylake, D.K. / Wojcik, E.J.

Funding support

United States, 1items

Citation

Journal: To Be Published
Title: Crystal Structure of the Klp61f motor domain bound to AMPPNP
Authors: Wojcik, E.J. / Worthylake, D.K.

History

Deposition	Mar 4, 2021	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Nov 16, 2022	Provider: repository / Type: Initial release
Revision 1.1	Oct 18, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7lxr.cif.gz	508.4 KB	Display	PDBx/mmCIF format
PDB format	pdb7lxr.ent.gz	407.4 KB	Display	PDB format
PDBx/mmJSON format	7lxr.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/lx/7lxr ftp://data.pdbj.org/pub/pdb/validation_reports/lx/7lxr	HTTPS FTP

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Related structure data

Related structure data	3hqdS S: Starting model for refinement
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#175 - Jul 2014 Microtubules similarity (9) #64 - Apr 2005 Kinesin similarity (7) #241 - Jan 2020 Twenty Years of Molecules similarity (9) #18 - Jun 2001 Myosin similarity (1) #174 - Jun 2014 GFP-like Proteins similarity (1) #108 - Dec 2008 Hsp90 similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #182 - Feb 2015 Insulin receptor similarity (1) #214 - Oct 2017 Chimeric Antigen Receptors similarity (1) #238 - Oct 2019 Ribonucleotide Reductase similarity (1) #43 - Jul 2003 Src Tyrosine Kinase similarity (1) #50 - Feb 2004 Glycolytic Enzymes similarity (1) #60 - Dec 2004 Ubiquitin similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #80 - Aug 2006 AAA+ Proteases similarity (1) #95 - Nov 2007 Multidrug Resistance Transporters similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#175 - Jul 2014
Microtubules
similarity (9)
#64 - Apr 2005
Kinesin
similarity (7)
#241 - Jan 2020
Twenty Years of Molecules
similarity (9)
#18 - Jun 2001
Myosin
similarity (1)
#174 - Jun 2014
GFP-like Proteins
similarity (1)
#108 - Dec 2008
Hsp90
similarity (1)
#168 - Dec 2013
DNA Helicase
similarity (1)
#182 - Feb 2015
Insulin receptor
similarity (1)
#214 - Oct 2017
Chimeric Antigen Receptors
similarity (1)
#238 - Oct 2019
Ribonucleotide Reductase
similarity (1)
#43 - Jul 2003
Src Tyrosine Kinase
similarity (1)
#50 - Feb 2004
Glycolytic Enzymes
similarity (1)
#60 - Dec 2004
Ubiquitin
similarity (1)
#73 - Jan 2006
Topoisomerase
similarity (1)
#80 - Aug 2006
AAA+ Proteases
similarity (1)
#95 - Nov 2007
Multidrug Resistance Transporters
similarity (1)

Deposited unit

A: Kinesin-like protein Klp61F

B: Kinesin-like protein Klp61F

hetero molecules

84.4 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Kinesin-like protein Klp61F

hetero molecules

defined by author&software
42.3 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Kinesin-like protein Klp61F

hetero molecules

defined by author&software
42.1 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	42.692, 50.813, 93.344
Angle α, β, γ (deg.)	78.532, 77.869, 73.197
Int Tables number	1
Space group name H-M	P1

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Protein , 1 types, 2 molecules AB

#1: Protein	Kinesin-like protein Klp61F / Bipolar kinesin KRP-130 Mass: 41425.965 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: Klp61F, KLP2, CG9191 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): BL21 Star (DE3) / References: UniProt: P46863

#1: Protein

Kinesin-like protein Klp61F / Bipolar kinesin KRP-130

Mass: 41425.965 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Drosophila melanogaster (fruit fly) / Gene: Klp61F, KLP2, CG9191 / Production host:

Escherichia coli BL21(DE3) (bacteria) / Variant (production host): BL21 Star (DE3) / References: UniProt: P46863

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Non-polymers , 5 types, 339 molecules

#2: Chemical	ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol Mass: 62.068 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₂H₆O₂
#3: Chemical	ChemComp-ANP / PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER Mass: 506.196 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₁₀H₁₇N₆O₁₂P₃ / Feature type: SUBJECT OF INVESTIGATION / Comment: AMP-PNP, energy-carrying molecule analogue*YM
#4: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#5: Chemical	ChemComp-IOD / IODIDE ION / Iodide Mass: 126.904 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: I
#6: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 329 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.27 Å³/Da / Density % sol: 45.75 %
Crystal grow	Temperature: 277.15 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 10% ethylene glycol, 50mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (sodium HEPES) pH 7.5, 180mM NaI and 15-17% polyethylene glycol MW 3,350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: ROTATING ANODE / Type: BRUKER AXS MICROSTAR / Wavelength: 1.5418 Å
Detector	Type: Bruker Platinum 135 / Detector: CCD / Date: Dec 29, 2012 / Details: Helios
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1.5418 Å / Relative weight: 1
Reflection	Resolution: 1.74→48.11 Å / Num. obs: 67986 / % possible obs: 91.8 % / Redundancy: 3.31 % / R_merge(I) obs: 0.0569 / Net I/σ(I): 12.56
Reflection shell	Resolution: 1.74→1.84 Å / R_merge(I) obs: 0.2722 / Mean I/σ(I) obs: 2.5 / Num. unique obs: 9356

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Processing

Software

Name	Version	Classification
REFMAC	5.8.0267	refinement
SAINT		data reduction
SAINT		data scaling
AMoRE		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 3HQD

3hqd

Resolution: 1.744→48.11 Å / Cor.coef. F_o:F_c: 0.956 / Cor.coef. F_o:F_c free: 0.937 / SU B: 5.203 / SU ML: 0.088 / Cross valid method: FREE R-VALUE / ESU R: 0.129 / ESU R Free: 0.124
Details: Hydrogens have been added in their riding positions

	R_factor	Num. reflection	% reflection
R_free	0.2238	2669	3.981 %
R_work	0.185	64375	-
all	0.187	-	-
obs	-	67044	90.616 %

Solvent computation

Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT

Displacement parameters

B_iso mean: 29.723 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	-0.593 Å²	0.341 Å²	0.235 Å²
2-	-	0.678 Å²	-0.956 Å²
3-	-	-	-0.119 Å²

Refinement step

Cycle: LAST / Resolution: 1.744→48.11 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	5431	0	82	329	5842

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.013	0.013	5663
X-RAY DIFFRACTION	r_bond_other_d	0.001	0.017	5503
X-RAY DIFFRACTION	r_angle_refined_deg	1.953	1.656	7681
X-RAY DIFFRACTION	r_angle_other_deg	1.457	1.583	12706
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	7.698	5	712
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	33.05	21.667	300
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	18.326	15	1072
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	16.63	15	49
X-RAY DIFFRACTION	r_chiral_restr	0.096	0.2	784
X-RAY DIFFRACTION	r_chiral_restr_other	0.08	0.2	2
X-RAY DIFFRACTION	r_gen_planes_refined	0.01	0.02	6272
X-RAY DIFFRACTION	r_gen_planes_other	0.001	0.02	1228
X-RAY DIFFRACTION	r_nbd_refined	0.234	0.2	1081
X-RAY DIFFRACTION	r_symmetry_nbd_other	0.207	0.2	5382
X-RAY DIFFRACTION	r_nbtor_refined	0.164	0.2	2685
X-RAY DIFFRACTION	r_symmetry_nbtor_other	0.089	0.2	2927
X-RAY DIFFRACTION	r_xyhbond_nbd_refined	0.204	0.2	313
X-RAY DIFFRACTION	r_symmetry_xyhbond_nbd_other	0.179	0.2	6
X-RAY DIFFRACTION	r_metal_ion_refined	0.091	0.2	3
X-RAY DIFFRACTION	r_symmetry_nbd_refined	0.271	0.2	22
X-RAY DIFFRACTION	r_nbd_other	0.257	0.2	96
X-RAY DIFFRACTION	r_symmetry_xyhbond_nbd_refined	0.328	0.2	20
X-RAY DIFFRACTION	r_xyhbond_nbd_other	0.371	0.2	2
X-RAY DIFFRACTION	r_mcbond_it	1.833	2.029	2773
X-RAY DIFFRACTION	r_mcbond_other	1.833	2.029	2772
X-RAY DIFFRACTION	r_mcangle_it	3.082	3.023	3468
X-RAY DIFFRACTION	r_mcangle_other	3.081	3.024	3469
X-RAY DIFFRACTION	r_scbond_it	2.311	2.372	2890
X-RAY DIFFRACTION	r_scbond_other	2.31	2.373	2891
X-RAY DIFFRACTION	r_scangle_it	3.755	3.424	4199
X-RAY DIFFRACTION	r_scangle_other	3.754	3.424	4200
X-RAY DIFFRACTION	r_lrange_it	6.508	24.722	6153
X-RAY DIFFRACTION	r_lrange_other	6.507	24.721	6154

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.744-1.79	0.288	157	0.272	3894	X-RAY DIFFRACTION	73.5476
1.79-1.839	0.275	172	0.251	4545	X-RAY DIFFRACTION	89.3371
1.839-1.892	0.296	172	0.226	4506	X-RAY DIFFRACTION	89.8924
1.892-1.95	0.247	170	0.209	4409	X-RAY DIFFRACTION	90.1378
1.95-2.014	0.226	202	0.185	4191	X-RAY DIFFRACTION	90.5586
2.014-2.085	0.214	168	0.178	4114	X-RAY DIFFRACTION	90.4329
2.085-2.164	0.198	162	0.176	3982	X-RAY DIFFRACTION	90.8971
2.164-2.252	0.204	161	0.16	3845	X-RAY DIFFRACTION	90.9008
2.252-2.352	0.23	150	0.173	3663	X-RAY DIFFRACTION	91.1111
2.352-2.467	0.202	135	0.178	3479	X-RAY DIFFRACTION	90.7812
2.467-2.6	0.239	143	0.172	3344	X-RAY DIFFRACTION	91.2111
2.6-2.758	0.231	142	0.174	3142	X-RAY DIFFRACTION	90.8186
2.758-2.948	0.236	132	0.178	3029	X-RAY DIFFRACTION	92.1037
2.948-3.184	0.229	130	0.182	2806	X-RAY DIFFRACTION	93.772
3.184-3.487	0.223	109	0.189	2711	X-RAY DIFFRACTION	96.6415
3.487-3.898	0.209	108	0.176	2504	X-RAY DIFFRACTION	98.715
3.898-4.499	0.2	88	0.163	2196	X-RAY DIFFRACTION	99.4773
4.499-5.507	0.199	84	0.171	1868	X-RAY DIFFRACTION	99.2374
5.507-7.774	0.331	50	0.227	1432	X-RAY DIFFRACTION	98.1457
7.774-48.11	0.17	34	0.212	715	X-RAY DIFFRACTION	89.2729

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.1785	-0.2721	-0.6968	0.6665	0.5428	0.9103	0.0314	0.0492	-0.1442	-0.1644	-0.0952	0.1214	-0.1198	-0.0777	0.0637	0.077	0.0089	0.0024	0.0796	-0.0058	0.0877	-3.0354	-2.0207	-10.1162
2	1.7109	-1.5546	-1.383	1.5931	1.2772	1.1228	0.0141	0.0259	0.0789	-0.0491	0.0494	-0.1078	-0.0191	-0.0058	-0.0634	0.0774	-0.0126	0.0336	0.0951	-0.036	0.0707	11.8829	-9.799	-22.6826
3	0.5797	-0.2407	0.6692	4.8336	1.3088	1.3327	-0.0381	0.1184	-0.0021	-0.0948	-0.059	0.1517	-0.1076	0.0699	0.0971	0.0752	0.0194	-0.0022	0.1616	-0.0576	0.0869	-6.1296	-4.2427	-21.0566
4	0.4926	0.1447	0.0103	0.4452	0.6285	0.9744	0.0143	0.1243	0.0172	-0.0801	-0.0538	0.0274	-0.1377	-0.1257	0.0395	0.0915	0.0168	-0.0088	0.0811	0.0035	0.0801	-3.4489	-1.4703	-16.398
5	0.1548	-0.1255	-0.1758	0.6357	0.2707	0.2383	0.0017	0.0521	-0.0035	-0.0262	-0.0318	0.0405	-0.0149	-0.0414	0.0301	0.0787	0.005	-0.0005	0.0729	0.0014	0.108	-0.3532	2.5063	-1.1839
6	2.7966	-0.1798	0.649	2.5612	0.0406	2.6818	0.053	-0.1286	0.3024	-0.3746	0.1071	-0.2877	-0.49	0.3216	-0.1601	0.15	-0.0839	0.0884	0.0741	-0.0161	0.1714	9.7622	15.7179	-6.3296
7	0.5361	-0.6406	-0.546	1.4488	0.8252	0.6012	-0.0143	0.0064	0.0763	0.0015	0.0642	-0.0369	0.0185	0.0104	-0.0499	0.0903	-0.0031	0.0068	0.0336	0.0078	0.1397	1.0285	17.0865	2.3743
8	0.3371	-0.2057	-0.2439	1.0793	0.3872	0.2608	-0.0284	-0.0713	0.0072	0.1593	-0.013	-0.0491	0.0511	0.0354	0.0415	0.1036	0.0121	-0.0227	0.0672	0.0055	0.1141	8.4826	1.3221	12.0618
9	5.3354	-1.336	1.3898	0.3512	-0.5289	2.9333	-0.5799	-0.5578	-0.5561	0.1245	0.1667	0.1032	-0.0332	-0.0777	0.4132	0.1302	-0.0109	0.0781	0.1602	0.084	0.1755	7.1204	-5.3445	20.6441
10	0.7718	-6.1813	-0.4194	49.6073	3.3595	0.2279	0.3098	0.0135	-0.0283	-2.3987	-0.335	0.2538	-0.1623	-0.0089	0.0252	0.1647	0.0059	-0.0767	0.549	-0.0658	0.0977	-11.3014	-2.26	18.9066
11	8.5659	4.3943	-3.5251	2.2595	-1.8076	1.4527	-0.0002	0.058	0.214	0.0134	0.0659	0.1204	-0.0033	-0.0169	-0.0656	0.1333	0.063	-0.012	0.1468	0.0612	0.0907	1.9168	-0.9036	18.1814
12	0.7102	-0.3065	-0.2097	1.9665	0.0967	0.3305	0.0152	-0.1278	0.2172	-0.0031	0.0462	-0.2386	0.0028	0.0856	-0.0614	0.0498	-0.0029	-0.0208	0.0623	-0.023	0.1436	11.9359	11.3695	8.0076
13	0.2502	0.0359	-0.1582	0.1484	0.092	0.3483	-0.0244	0.0138	-0.0021	0.0424	-0.0209	-0.0386	0.0056	0.0422	0.0454	0.0796	-0.0045	-0.0049	0.0763	0.0102	0.1169	9.0495	0.2543	3.0511
14	4.9415	3.6358	2.2487	4.8088	-0.1027	6.9639	-0.073	0.0066	-0.111	0.0385	-0.2774	-0.05	-0.2253	-0.3004	0.3503	0.0851	0.0183	0.0203	0.1047	-0.0543	0.0892	-13.735	21.9198	17.1988
15	1.1564	-1.7861	-1.4202	3.417	2.4489	1.8436	-0.0564	-0.0152	-0.0882	0.1355	-0.047	0.1064	0.0896	-0.0083	0.1034	0.0867	0.0042	0.0044	0.067	-0.0011	0.1102	0.0368	0.0022	4.2489
16	2.9616	-0.1577	0.2776	0.0471	0.0107	0.0604	0.0167	0.0921	-0.1613	0.0599	-0.0078	-0.0105	0.0634	-0.0136	-0.0089	0.1279	-0.0154	0.0185	0.0509	0.0078	0.1606	7.0717	-17.1206	2.2917
17	1.2681	0.4549	0.3006	0.7689	0.1081	0.4748	0.0071	-0.0958	-0.053	0.1319	-0.0724	0.0858	0.0339	0.0159	0.0654	0.0781	-0.0345	0.0382	0.0575	0.0202	0.1242	-7.8804	-11.5443	7.4077
18	0.639	-0.2515	-0.5481	0.8269	0.7022	0.7975	-0.0117	0.0439	-0.028	-0.0304	-0.0106	0.0305	-0.0188	-0.023	0.0223	0.0702	0.0034	0.0079	0.0824	-0.0175	0.1009	-0.936	-7.2894	-8.6256
19	2.9936	-1.0752	-1.7077	0.5676	0.9869	1.7599	-0.0416	0.1103	-0.0706	0.0584	-0.0488	0.0618	0.1239	-0.1136	0.0904	0.0582	-0.0126	0.0185	0.0723	-0.0091	0.153	-9.2105	-9.937	-6.8518
20	1.6106	3.3173	2.458	7.4535	6.5831	7.5192	0.1636	-0.1967	0.0779	0.2055	-0.3964	0.2137	-0.0032	-0.2822	0.2329	0.0993	-0.0315	0.0249	0.0869	-0.0384	0.125	-14.801	9.1198	13.9495
21	0.664	0.7965	0.7505	1.8376	0.6086	0.9955	0.1076	0.2534	-0.0921	0.028	0.0115	-0.0781	0.2316	0.3595	-0.1191	0.1057	0.0603	-0.0163	0.2056	-0.0762	0.0828	-2.2323	-21.0898	36.9517
22	3.3101	1.4607	1.4954	0.9776	1.5592	3.1379	0.0099	-0.2909	-0.1538	0.0272	-0.0818	-0.0386	0.1222	-0.1176	0.0719	0.1132	-0.0283	0.0093	0.102	-0.0518	0.0761	-14.7536	-31.2338	24.4452
23	0.2594	-0.5531	0.6565	1.4947	-0.2165	6.369	-0.0086	-0.0199	0.0023	0.0045	0.0813	-0.0506	0.1601	0.0989	-0.0728	0.0962	0.0242	0.0186	0.0837	-0.0618	0.1008	3.008	-29.0134	32.7819
24	0.8473	1.1341	-0.1067	1.5305	-0.1507	0.021	0.0249	-0.1107	-0.0512	0.0006	-0.0781	-0.0939	0.0137	-0.0095	0.0532	0.0881	0.0191	0.0429	0.2464	-0.0638	0.104	2.347	-30.9224	16.6838
25	0.9447	-0.9887	-0.9426	1.0849	1.0587	1.0452	-0.0331	-0.0756	0.0519	0.125	0.2036	-0.1548	0.1578	0.2185	-0.1706	0.1395	0.1166	-0.1151	0.221	-0.2166	0.2129	1.3029	-25.9483	33.7063
26	0.1694	0.1904	0.166	0.5509	0.2812	1.1063	0.0474	0.1073	-0.0937	0.0429	0.041	-0.1251	0.0769	0.0855	-0.0884	0.0791	0.018	-0.0272	0.1079	-0.0374	0.069	-5.7257	-17.4929	45.8186
27	2.145	0.1902	-5.2232	2.7734	-0.9017	12.8408	-0.1252	0.1013	0.1263	-0.1676	0.412	-0.2315	0.4403	-0.3667	-0.2867	0.1518	-0.0924	-0.026	0.1813	0.0392	0.0666	-19.9008	-23.9558	55.5044
28	4.9457	4.5657	5.8313	9.0422	8.7398	9.2141	0.1247	0.0553	-0.1648	0.5952	0.0657	-0.1334	0.5099	0.0829	-0.1905	0.1856	-0.0158	-0.0327	0.0236	0.0377	0.0739	-9.9024	-27.3367	54.5169
29	0.5802	0.4045	0.1929	0.4479	0.2102	0.6112	0.0516	-0.0423	-0.0237	0.0533	-0.0398	-0.0298	0.0289	-0.1953	-0.0117	0.1087	0.0137	-0.0078	0.0814	-0.0063	0.023	-12.7092	-10.2807	53.2373
30	9.9603	-0.0456	-5.6247	1.6494	-0.8415	3.6906	-0.1405	0.3137	0.1123	0.0175	0.1369	-0.0466	-0.0339	-0.2576	0.0036	0.1933	0.0221	-0.0647	0.0651	0.0289	0.0803	-18.8644	7.3983	44.78
31	20.9701	-5.1014	2.3577	5.6334	-2.017	0.7396	0.256	-0.6072	-0.3481	-0.1379	-0.2398	-0.0316	0.0507	0.0548	-0.0162	0.1417	-0.0211	-0.0103	0.062	0.0571	0.1488	0.6337	7.9845	46.9747
32	0.6375	0.0583	-0.1192	0.2233	0.391	1.6292	0.0488	0.0021	0.0433	0.0748	-0.0565	0.0656	-0.0539	-0.2368	0.0077	0.0898	0.0198	0.0128	0.0919	-0.0155	0.0634	-18.4229	-7.1492	50.6475
33	0.6625	-0.3829	-0.2037	3.7734	2.6885	1.9294	0.0418	0.1491	-0.046	0.0193	-0.2637	0.3132	-0.0047	-0.1675	0.2218	0.1014	0.0205	-0.0141	0.1621	-0.0169	0.0413	-20.3954	-15.1036	33.8033
34	0.4715	0.2338	0.3546	0.2396	0.4895	1.1171	0.0605	-0.0003	0.0409	0.028	0.0042	-0.0315	0.0946	0.0057	-0.0647	0.0991	0.005	-0.0049	0.1219	-0.0123	0.0802	-3.8269	-6.2274	57.3507
35	1.1154	0.0301	-0.2295	0.0272	-0.124	1.1015	-0.0268	0.0979	0.0995	-0.0214	0.0107	0.0042	-0.1944	0.0792	0.0161	0.1846	-0.0353	-0.0192	0.1916	0.0483	0.0179	-11.0127	-6.1477	27.3527
36	6.0653	-0.323	-2.7188	1.8577	1.3921	2.0641	0.1543	-0.1276	0.4669	-0.6653	0.2694	-0.3081	-0.5228	0.2468	-0.4237	0.2429	-0.1321	0.1009	0.2293	0.0375	0.1248	3.243	2.2642	31.9453
37	4.1989	-0.9033	1.8126	2.4469	-0.3052	0.7891	-0.1397	0.2424	0.0428	-0.0186	0.1335	0.022	-0.0788	0.1245	0.0062	0.142	-0.0532	0.0501	0.1603	0.043	0.0453	-4.6088	-0.9614	38.6939
38	0.0648	0.2682	0.3071	1.1745	1.0498	3.0514	-0.0263	0.0017	-0.008	-0.0893	0.0323	-0.0524	0.0411	0.1611	-0.0061	0.0606	0.0098	0.0064	0.1379	-0.0561	0.0653	-3.8732	-17.68	31.5525
39	2.0169	1.1832	2.8506	1.8707	2.0032	4.1325	-0.081	0.2708	-0.0617	0.0132	0.1851	-0.0127	-0.1022	0.4211	-0.1041	0.0139	-0.0439	0.017	0.2147	-0.0445	0.0486	6.0625	-10.7931	36.251
40	0.7184	0.4074	1.1812	0.4635	1.7717	7.1997	-0.0465	0.0155	0.059	-0.0403	0.0464	0.0118	-0.1249	0.1427	0.0001	0.0792	-0.0154	-0.014	0.0971	-0.0085	0.086	4.816	0.2445	61.101

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	ALL	A	18 - 29
2	X-RAY DIFFRACTION	2	ALL	A	30 - 42
3	X-RAY DIFFRACTION	3	ALL	A	43 - 53
4	X-RAY DIFFRACTION	4	ALL	A	54 - 87
5	X-RAY DIFFRACTION	5	ALL	A	88 - 115
6	X-RAY DIFFRACTION	6	ALL	A	116 - 130
7	X-RAY DIFFRACTION	7	ALL	A	131 - 151
8	X-RAY DIFFRACTION	8	ALL	A	152 - 173
9	X-RAY DIFFRACTION	9	ALL	A	174 - 182
10	X-RAY DIFFRACTION	10	ALL	A	183 - 188
11	X-RAY DIFFRACTION	11	ALL	A	189 - 196
12	X-RAY DIFFRACTION	12	ALL	A	197 - 216
13	X-RAY DIFFRACTION	13	ALL	A	217 - 242
14	X-RAY DIFFRACTION	14	ALL	A	243 - 251
15	X-RAY DIFFRACTION	15	ALL	A	252 - 268
16	X-RAY DIFFRACTION	16	ALL	A	274 - 296
17	X-RAY DIFFRACTION	17	ALL	A	297 - 323
18	X-RAY DIFFRACTION	18	ALL	A	324 - 343
19	X-RAY DIFFRACTION	19	ALL	A	344 - 358
20	X-RAY DIFFRACTION	20	ALL	A	359 - 367
21	X-RAY DIFFRACTION	21	ALL	B	18 - 29
22	X-RAY DIFFRACTION	22	ALL	B	30 - 42
23	X-RAY DIFFRACTION	23	ALL	B	43 - 52
24	X-RAY DIFFRACTION	24	ALL	B	53 - 60
25	X-RAY DIFFRACTION	25	ALL	B	61 - 74
26	X-RAY DIFFRACTION	26	ALL	B	75 - 115
27	X-RAY DIFFRACTION	27	ALL	B	116 - 123
28	X-RAY DIFFRACTION	28	ALL	B	124 - 129
29	X-RAY DIFFRACTION	29	ALL	B	130 - 172
30	X-RAY DIFFRACTION	30	ALL	B	173 - 181
31	X-RAY DIFFRACTION	31	ALL	B	182 - 187
32	X-RAY DIFFRACTION	32	ALL	B	188 - 221
33	X-RAY DIFFRACTION	33	ALL	B	222 - 233
34	X-RAY DIFFRACTION	34	ALL	B	234 - 265
35	X-RAY DIFFRACTION	35	ALL	B	266 - 301
36	X-RAY DIFFRACTION	36	ALL	B	302 - 310
37	X-RAY DIFFRACTION	37	ALL	B	311 - 323
38	X-RAY DIFFRACTION	38	ALL	B	324 - 348
39	X-RAY DIFFRACTION	39	ALL	B	349 - 360
40	X-RAY DIFFRACTION	40	ALL	B	361 - 367

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