Details | Type | Solution-ID | Contents | Label | Solvent system |
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solution1 | 250 uM 1H RNA (103-MER), 100% D2O | 1H_sample100% D2Osolution2 | 250 uM A-8D, C-D, G-H, U-5,6-D2 RNA (103-MER), 100% D2O | A-8D, C-D, G-H, U-5,6-D2 | 100% D2Osolution3 | 250 uM A-8D, C-D, G-8D, U-H RNA (103-MER), 100% D2O | A-8D, C-D, G-8D, U-H | 100% D2Osolution4 | 250 uM A-8D, C-5,6-D2, G-D, U-5,6-D2 RNA (103-MER), 100% D2O | A-8D, C-5,6-D2, G-D, U-5,6-D2 | 100% D2Osolution5 | 250 uM A-8D, C-D, G-8D, U-D RNA (103-MER), 100% D2O | A-8D, C-D, G-8D, U-D | 100% D2Osolution6 | 250 uM A-H, C-D, G-H, U-D RNA (103-MER), 100% D2O | A-H, C-D, G-H, U-D | 100% D2Osolution7 | 250 uM A-H, C-H, G-D, U-D RNA (103-MER), 100% D2O | A-H, C-H, G-D, U-D | 100% D2O | | | | | | | | | | | | | | |
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Sample | Conc. (mg/ml) | Component | Isotopic labeling | Solution-ID |
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250 uMRNA (103-MER) | 1H1 | 250 uMRNA (103-MER) | A-8D, C-D, G-H, U-5,6-D2 | 2 | 250 uMRNA (103-MER) | A-8D, C-D, G-8D, U-H | 3 | 250 uMRNA (103-MER) | A-8D, C-5,6-D2, G-D, U-5,6-D2 | 4 | 250 uMRNA (103-MER) | A-8D, C-D, G-8D, U-D | 5 | 250 uMRNA (103-MER) | A-H, C-D, G-H, U-D | 6 | 250 uMRNA (103-MER) | A-H, C-H, G-D, U-D | 7 | | | | | | | | |
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Sample conditions | Ionic strength: 3 mM / Label: condition_1 / pH: 7.5 pD / Pressure: 1 atm / Temperature: 308 K |
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