National Health and Medical Research Council (NHMRC, Australia)
APP1164216
オーストラリア
引用
ジャーナル: Nat Commun / 年: 2021 タイトル: A unified route for flavivirus structures uncovers essential pocket factors conserved across pathogenic viruses. 著者: Joshua M Hardy / Natalee D Newton / Naphak Modhiran / Connor A P Scott / Hariprasad Venugopal / Laura J Vet / Paul R Young / Roy A Hall / Jody Hobson-Peters / Fasséli Coulibaly / Daniel Watterson / 要旨: The epidemic emergence of relatively rare and geographically isolated flaviviruses adds to the ongoing disease burden of viruses such as dengue. Structural analysis is key to understand and combat ...The epidemic emergence of relatively rare and geographically isolated flaviviruses adds to the ongoing disease burden of viruses such as dengue. Structural analysis is key to understand and combat these pathogens. Here, we present a chimeric platform based on an insect-specific flavivirus for the safe and rapid structural analysis of pathogenic viruses. We use this approach to resolve the architecture of two neurotropic viruses and a structure of dengue virus at 2.5 Å, the highest resolution for an enveloped virion. These reconstructions allow improved modelling of the stem region of the envelope protein, revealing two lipid-like ligands within highly conserved pockets. We show that these sites are essential for viral growth and important for viral maturation. These findings define a hallmark of flavivirus virions and a potential target for broad-spectrum antivirals and vaccine design. We anticipate the chimeric platform to be widely applicable for investigating flavivirus biology.
M and E from Murray Valley encephalitis virus strain MVE-1-51 form a complex that assembles into anti-parallel dimers, (M-E)2, in the T=3 icosahedral particle.
COMPLEX
The prM and E genes of Murray Valley encephalitis virus strain MVE-1-51 were integrated into the Binjari virus genome using the Circular Polymerase Extension Reaction
#1-#2
1
RECOMBINANT
分子量
ID
Entity assembly-ID
値 (°)
実験値
1
1
22MDa
NO
2
1
11.2MDa
NO
由来(天然)
ID
Entity assembly-ID
生物種
Ncbi tax-ID
株
1
1
Binjari virus (ウイルス)
2305258
BFTA20
2
2
Murray Valley encephalitis virus (マレーバレー脳炎ウイルス)
11079
由来(組換発現)
生物種: Aedes albopictus (ヒトスジシマカ) / 細胞: C6/36
ウイルスについての詳細
中空か: NO / エンベロープを持つか: YES / 単離: SPECIES / タイプ: VIRION
天然宿主
生物種: Ochlerotatus normanensis
ウイルス殻
直径: 470 nm / 三角数 (T数): 3
緩衝液
pH: 8
緩衝液成分
ID
濃度
名称
式
Buffer-ID
1
12mM
Tris
(HOCH2)3CNH2
1
2
120mM
sodiumchloride
NaCl
1
3
1mM
Ethylenediaminetetraaceticacid (EDTA)
C10H16N2O8
1
試料
濃度: 4 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES