+Open data
-Basic information
Entry | Database: PDB / ID: 7ji3 | ||||||
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Title | Cryo-EM structure of a proton-activated chloride channel | ||||||
Components | Proton-activated chloride channel | ||||||
Keywords | TRANSPORT PROTEIN / ion channel | ||||||
Function / homology | TMEM206 protein / TMEM206 protein family / membrane => GO:0016020 / plasma membrane / Proton-activated chloride channel Function and homology information | ||||||
Biological species | Takifugu bimaculatus (fish) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.46 Å | ||||||
Authors | Deng, Z. / Zhang, J. / Yuan, P. | ||||||
Citation | Journal: Sci Adv / Year: 2021 Title: Cryo-EM structure of a proton-activated chloride channel TMEM206. Authors: Zengqin Deng / Yonghui Zhao / Jing Feng / Jingying Zhang / Haiyan Zhao / Michael J Rau / James A J Fitzpatrick / Hongzhen Hu / Peng Yuan / Abstract: TMEM206 has been recently identified as an evolutionarily conserved chloride channel that underlies ubiquitously expressed, proton-activated, outwardly rectifying anion currents. Here, we report the ...TMEM206 has been recently identified as an evolutionarily conserved chloride channel that underlies ubiquitously expressed, proton-activated, outwardly rectifying anion currents. Here, we report the cryo-electron microscopy structure of pufferfish TMEM206, which forms a trimeric channel, with each subunit comprising two transmembrane segments and a large extracellular domain. An ample vestibule in the extracellular region is accessible laterally from the three side portals. The central pore contains multiple constrictions. A conserved lysine residue near the cytoplasmic end of the inner helix forms the presumed chloride ion selectivity filter. Unprecedentedly, the core structure and assembly closely resemble those of the epithelial sodium channel/degenerin family of sodium channels that are unrelated in amino acid sequence and conduct cations instead of anions. Together with electrophysiology, this work provides insights into ion conduction and gating for a new class of chloride channels that is architecturally distinct from previously characterized chloride channel families. | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7ji3.cif.gz | 144.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7ji3.ent.gz | 116.4 KB | Display | PDB format |
PDBx/mmJSON format | 7ji3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7ji3_validation.pdf.gz | 711 KB | Display | wwPDB validaton report |
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Full document | 7ji3_full_validation.pdf.gz | 716.6 KB | Display | |
Data in XML | 7ji3_validation.xml.gz | 26.6 KB | Display | |
Data in CIF | 7ji3_validation.cif.gz | 36 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ji/7ji3 ftp://data.pdbj.org/pub/pdb/validation_reports/ji/7ji3 | HTTPS FTP |
-Related structure data
Related structure data | 22343MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 52907.641 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Takifugu bimaculatus (fish) / Gene: fugu_017091 / Production host: Komagataella pastoris (fungus) / References: UniProt: A0A4Z2BWB0*PLUS |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: TMEM206 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Takifugu rubripes (torafugu) |
Source (recombinant) | Organism: pichia pas (fungus) |
Buffer solution | pH: 8 |
Specimen | Conc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company | ||||||||||||
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Microscopy | Model: FEI TITAN KRIOS | ||||||||||||
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER | ||||||||||||
Electron lens | Mode: OTHER | ||||||||||||
Image recording |
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-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 3.46 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 505115 / Symmetry type: POINT |