温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7 詳細: 10-12 mg/mL protein in 25 mM Tris/HCl pH7.5, 500mM NaCl, 2mM TCEP, supplemented with 10x molar excess of ligand and, if needed, with 10 mM MgCl2 and 5mM ATP, then mixed 1:1 with reservoir of ...詳細: 10-12 mg/mL protein in 25 mM Tris/HCl pH7.5, 500mM NaCl, 2mM TCEP, supplemented with 10x molar excess of ligand and, if needed, with 10 mM MgCl2 and 5mM ATP, then mixed 1:1 with reservoir of the Procomplex screen. Several conditions resulted in crystals.
構造決定の手法: 分子置換 開始モデル: inhouse model 解像度: 2→82.03 Å / Cor.coef. Fo:Fc: 0.941 / Cor.coef. Fo:Fc free: 0.925 / SU R Cruickshank DPI: 0.227 / 交差検証法: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.228 / SU Rfree Blow DPI: 0.196 / SU Rfree Cruickshank DPI: 0.198 詳細: ligand well defined by electron density. 19F library NMR fragment hit