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Open data
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Basic information
Entry | Database: PDB / ID: 7fga | ||||||
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Title | Alpha-1,2-glucosyltransferase_UDP_sucrose_tll1591 | ||||||
![]() | Glycosyl transferase | ||||||
![]() | TRANSFERASE / alpha-1 / 2-glucosyltransferase_UDP_sucrose_tll1591 | ||||||
Function / homology | Glycosyl transferase 4-like domain / Glycosyltransferase subfamily 4-like, N-terminal domain / Glycosyl transferase, family 1 / Glycosyl transferases group 1 / glycosyltransferase activity / surcrose isoform / URIDINE-5'-DIPHOSPHATE / Glycosyl transferase![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Su, J.Y. | ||||||
![]() | ![]() Title: Structural basis for glucosylsucrose synthesis by a member of the alpha-1,2-glucosyltransferase family Authors: Han, Q. / Yao, Y. / Liu, Y. / Zhang, W. / Yu, J. / Na, H. / Liu, T. / Mayo, K. / Su, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 280.7 KB | Display | ![]() |
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PDB format | ![]() | 226.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.9 MB | Display | ![]() |
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Full document | ![]() | 2.9 MB | Display | |
Data in XML | ![]() | 33.3 KB | Display | |
Data in CIF | ![]() | 46.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7fg9SC S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 40407.930 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: BP-1 / Gene: tll1591 / Production host: ![]() ![]() #2: Polysaccharide | alpha-D-glucopyranose-(1-1)-alpha-D-fructofuranose #3: Chemical | ChemComp-UDP / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.17 Å3/Da / Density % sol: 70.51 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 21, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→19.96 Å / Num. obs: 43413 / % possible obs: 99.4 % / Redundancy: 5.8 % / Rmerge(I) obs: 0.19 / Net I/σ(I): 6.3 |
Reflection shell | Resolution: 3.2→3.37 Å / Rmerge(I) obs: 0.99 / Num. unique obs: 6364 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7FG9 Resolution: 3.2→19.96 Å / SU ML: 0.67 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 37.28 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 145.89 Å2 / Biso mean: 64.6819 Å2 / Biso min: 28.16 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.2→19.96 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14
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