+Open data
-Basic information
Entry | Database: PDB / ID: 7f7p | ||||||
---|---|---|---|---|---|---|---|
Title | AcrIIC4 | ||||||
Components | anti-CRISPR protein AcrIIC4 | ||||||
Keywords | IMMUNE SYSTEM / Anti-CRISPR AcrIIC4 / Inhibitor of CRISPR-Cas 9 | ||||||
Function / homology | Uncharacterized protein Function and homology information | ||||||
Biological species | Haemophilus parainfluenzae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / AB INITIO PHASING / Resolution: 2.03 Å | ||||||
Authors | Kim, G.E. / Park, H.H. | ||||||
Funding support | Korea, Republic Of, 1items
| ||||||
Citation | Journal: Protein Sci. / Year: 2021 Title: Crystal structure of the anti-CRISPR, AcrIIC4. Authors: Kim, G.E. / Lee, S.Y. / Park, H.H. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7f7p.cif.gz | 49.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7f7p.ent.gz | 34.7 KB | Display | PDB format |
PDBx/mmJSON format | 7f7p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7f7p_validation.pdf.gz | 432 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 7f7p_full_validation.pdf.gz | 434.5 KB | Display | |
Data in XML | 7f7p_validation.xml.gz | 8.9 KB | Display | |
Data in CIF | 7f7p_validation.cif.gz | 11.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f7/7f7p ftp://data.pdbj.org/pub/pdb/validation_reports/f7/7f7p | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 11056.469 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Haemophilus parainfluenzae (bacteria) / Gene: NCTC10672_00033, NCTC10672_02354 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A377JKY9 #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 41.18 % |
---|---|
Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop Details: 0.1 M CHES/Sodium hydroxide pH 9.5 30 % w/v PEG 3000 |
-Data collection
Diffraction | Mean temperature: 125 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 5C (4A) / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Dec 1, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→29.26 Å / Num. obs: 11895 / % possible obs: 99.84 % / Redundancy: 6.8 % / Rrim(I) all: 0.097 / Net I/σ(I): 11.5 |
Reflection shell | Resolution: 2.03→2.103 Å / Num. unique obs: 1183 / CC1/2: 0.669 |
-Processing
Software |
| |||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 2.03→29.26 Å / SU ML: 0.19 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 28.97 / Stereochemistry target values: ML
| |||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.03→29.26 Å
| |||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||
LS refinement shell |
|