デオキシリボ核酸 / DNA (> 10) / リボ核酸 / RNA (> 10) / DNA-directed RNA polymerases II and IV subunit 5A / RNA-dependent RNA polymerase 2 / DNA-directed RNA polymerases II, IV and V subunit 11 / DNA-directed RNA polymerases II, IV and V subunit 3 / DNA-directed RNA polymerases II, IV and V subunit 9A / DNA-directed RNA polymerases II, IV and V subunit 10 ...デオキシリボ核酸 / DNA (> 10) / リボ核酸 / RNA (> 10) / DNA-directed RNA polymerases II and IV subunit 5A / RNA-dependent RNA polymerase 2 / DNA-directed RNA polymerases II, IV and V subunit 11 / DNA-directed RNA polymerases II, IV and V subunit 3 / DNA-directed RNA polymerases II, IV and V subunit 9A / DNA-directed RNA polymerases II, IV and V subunit 10 / DNA-directed RNA polymerases II, IV and V subunit 6A / DNA-directed RNA polymerases II, IV and V subunit 12 / DNA-directed RNA polymerases IV and V subunit 2 / DNA-directed RNA polymerases II, IV and V subunit 8B 類似検索 - 構成要素
National Natural Science Foundation of China (NSFC)
31822001
中国
引用
ジャーナル: Science / 年: 2021 タイトル: Pol IV and RDR2: A two-RNA-polymerase machine that produces double-stranded RNA. 著者: Kun Huang / Xiao-Xian Wu / Cheng-Li Fang / Zhou-Geng Xu / Hong-Wei Zhang / Jian Gao / Chuan-Miao Zhou / Lin-Lin You / Zhan-Xi Gu / Wen-Hui Mu / Yu Feng / Jia-Wei Wang / Yu Zhang / 要旨: DNA methylation affects gene expression and maintains genome integrity. The DNA-dependent RNA polymerase IV (Pol IV), together with the RNA-dependent RNA polymerase RDR2, produces double-stranded ...DNA methylation affects gene expression and maintains genome integrity. The DNA-dependent RNA polymerase IV (Pol IV), together with the RNA-dependent RNA polymerase RDR2, produces double-stranded small interfering RNA precursors essential for establishing and maintaining DNA methylation in plants. We determined the cryo–electron microscopy structures of the Pol IV–RDR2 holoenzyme and the backtracked transcription elongation complex. These structures reveal that Pol IV and RDR2 form a complex with their active sites connected by an interpolymerase channel, through which the Pol IV–generated transcript is handed over to the RDR2 active site after being backtracked, where it is used as the template for double-stranded RNA (dsRNA) synthesis. Our results describe a ‘backtracking-triggered RNA channeling’ mechanism underlying dsRNA synthesis and also shed light on the evolutionary trajectory of eukaryotic RNA polymerases.
#241 - 2020年1月 20年の分子を振り返って (Twenty Years of Molecules) 類似性 (48)
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集合体
登録構造単位
A: DNA-directed RNA polymerase IV subunit 1 B: DNA-directed RNA polymerases IV and V subunit 2 C: DNA-directed RNA polymerases II, IV and V subunit 3 E: DNA-directed RNA polymerases II and IV subunit 5A F: DNA-directed RNA polymerases II, IV and V subunit 6A H: DNA-directed RNA polymerases II, IV and V subunit 8B I: DNA-directed RNA polymerases II, IV and V subunit 9A J: DNA-directed RNA polymerases II, IV and V subunit 10 K: DNA-directed RNA polymerases II, IV and V subunit 11 L: DNA-directed RNA polymerases II, IV and V subunit 12 M: RNA-dependent RNA polymerase 2 N: DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*TP*AP*TP*CP*GP*GP*TP*AP*GP*AP*GP*TP*G)-3') O: RNA (39-MER) Q: DNA (33-MER) R: RNA (5'-R(*CP*CP*GP*A)-3') ヘテロ分子
DNA-directedRNApolymerasesIVandVsubunit2 / DNA-directed RNA polymerase D subunit 2a / AtNRPD2a / Nuclear RNA polymerase D 2a / Nuclear RNA ...DNA-directed RNA polymerase D subunit 2a / AtNRPD2a / Nuclear RNA polymerase D 2a / Nuclear RNA polymerase E 2 / Protein DEFECTIVE IN MERISTEM SILENCING 2 / Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 2 / RNA polymerase IV subunit 2a / POL IV 2a
DNA-directedRNApolymerasesIIandIVsubunit5A / RPB5a / DNA-directed RNA polymerase II subunit E / RNA polymerase I / II and III 24.3 kDa subunit / AtRPB24.3