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- PDB-7eqc: Crystal structure of the mini-centralspindlin complex -

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Basic information

Entry
Database: PDB / ID: 7eqc
TitleCrystal structure of the mini-centralspindlin complex
Components
  • CYtoKinesis defect
  • Kinesin-like protein
KeywordsCELL CYCLE / Heterotetramer / Centralspindlin complex / assembly / molecular backbone
Function / homology
Function and homology information


spindle midzone assembly / CDC42 GTPase cycle / RAC2 GTPase cycle / RHOD GTPase cycle / RAC3 GTPase cycle / RAC1 GTPase cycle / pronuclear migration / COPI-dependent Golgi-to-ER retrograde traffic / RHOA GTPase cycle / RHOB GTPase cycle ...spindle midzone assembly / CDC42 GTPase cycle / RAC2 GTPase cycle / RHOD GTPase cycle / RAC3 GTPase cycle / RAC1 GTPase cycle / pronuclear migration / COPI-dependent Golgi-to-ER retrograde traffic / RHOA GTPase cycle / RHOB GTPase cycle / Kinesins / microtubule bundle formation involved in mitotic spindle midzone assembly / regulation of actomyosin contractile ring contraction / centralspindlin complex / meiotic spindle midzone assembly / mitotic cleavage furrow ingression / polar body extrusion after meiotic divisions / mitotic spindle midzone assembly / mitotic spindle midzone / plus-end-directed microtubule motor activity / microtubule motor activity / kinesin complex / microtubule-based movement / mitotic sister chromatid segregation / cleavage furrow / mitotic cytokinesis / Rho protein signal transduction / spindle midzone / GTPase activator activity / spindle / midbody / microtubule binding / microtubule / centrosome / protein kinase binding / ATP hydrolysis activity / ATP binding / identical protein binding / nucleus / metal ion binding / plasma membrane / cytoplasm
Similarity search - Function
Kinesin-like protein Kif23, Arf6-interacting domain / Kinesin-like protein Kif23, Arf6-interacting domain superfamily / Arf6-interacting domain of mitotic kinesin-like protein 1 / Kinesin-like protein / Rho GTPase-activating protein domain / RhoGAP domain / Rho GTPase-activating proteins domain profile. / GTPase-activator protein for Rho-like GTPases / Phorbol esters/diacylglycerol binding domain (C1 domain) / Rho GTPase activation protein ...Kinesin-like protein Kif23, Arf6-interacting domain / Kinesin-like protein Kif23, Arf6-interacting domain superfamily / Arf6-interacting domain of mitotic kinesin-like protein 1 / Kinesin-like protein / Rho GTPase-activating protein domain / RhoGAP domain / Rho GTPase-activating proteins domain profile. / GTPase-activator protein for Rho-like GTPases / Phorbol esters/diacylglycerol binding domain (C1 domain) / Rho GTPase activation protein / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain profile. / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Kinesin motor domain / Zinc finger phorbol-ester/DAG-type signature. / Zinc finger phorbol-ester/DAG-type profile. / Protein kinase C-like, phorbol ester/diacylglycerol-binding domain / C1-like domain superfamily / Kinesin motor domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Kinesin-like protein / Rac GTPase-activating protein 1
Similarity search - Component
Biological speciesCaenorhabditis elegans (invertebrata)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SIRAS / Resolution: 2.5 Å
AuthorsChen, Z. / Pan, H.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2021
Title: Mechanistic insights into central spindle assembly mediated by the centralspindlin complex.
Authors: Pan, H. / Guan, R. / Zhao, R. / Ou, G. / Chen, Z.
History
DepositionMay 1, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Apr 13, 2022Provider: repository / Type: Initial release
Revision 1.1May 29, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: CYtoKinesis defect
C: CYtoKinesis defect
J: Kinesin-like protein
F: Kinesin-like protein
D: CYtoKinesis defect
E: Kinesin-like protein
G: CYtoKinesis defect
I: Kinesin-like protein


Theoretical massNumber of molelcules
Total (without water)119,7328
Polymers119,7328
Non-polymers00
Water68538
1
B: CYtoKinesis defect
C: CYtoKinesis defect
E: Kinesin-like protein
I: Kinesin-like protein


Theoretical massNumber of molelcules
Total (without water)59,8664
Polymers59,8664
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area16000 Å2
ΔGint-144 kcal/mol
Surface area24650 Å2
MethodPISA
2
J: Kinesin-like protein
F: Kinesin-like protein
D: CYtoKinesis defect
G: CYtoKinesis defect


Theoretical massNumber of molelcules
Total (without water)59,8664
Polymers59,8664
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area16800 Å2
ΔGint-153 kcal/mol
Surface area25740 Å2
MethodPISA
Unit cell
Length a, b, c (Å)64.756, 65.406, 75.789
Angle α, β, γ (deg.)96.420, 92.240, 105.030
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain B and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))
21(chain C and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))
31(chain D and (resid 9 through 91 or resid 93 through 109))
41(chain G and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))
12(chain F and resid 439 through 545)
22(chain I and (resid 439 through 459 or resid 466 through 497 or resid 506 through 545))
32(chain J and (resid 439 through 459 or resid 466 through 497 or resid 506 through 545))

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111LYSLYSASNASN(chain B and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))BA9 - 2413 - 28
121PROPROLYSLYS(chain B and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))BA28 - 9132 - 95
131LEULEUVALVAL(chain B and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))BA93 - 10997 - 113
211LYSLYSASNASN(chain C and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))CB9 - 2413 - 28
221PROPROLYSLYS(chain C and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))CB28 - 9132 - 95
231LEULEUVALVAL(chain C and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))CB93 - 10997 - 113
311LYSLYSLYSLYS(chain D and (resid 9 through 91 or resid 93 through 109))DE9 - 9113 - 95
321LEULEUVALVAL(chain D and (resid 9 through 91 or resid 93 through 109))DE93 - 10997 - 113
411LYSLYSASNASN(chain G and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))GG9 - 2413 - 28
421PROPROLYSLYS(chain G and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))GG28 - 9132 - 95
431LEULEUVALVAL(chain G and (resid 9 through 24 or resid 28 through 91 or resid 93 through 109))GG93 - 10997 - 113
112ARGARGGLYGLY(chain F and resid 439 through 545)FD439 - 54518 - 124
212ARGARGGLUGLU(chain I and (resid 439 through 459 or resid 466 through 497 or resid 506 through 545))IH439 - 45918 - 38
222ILEILEGLNGLN(chain I and (resid 439 through 459 or resid 466 through 497 or resid 506 through 545))IH466 - 49745 - 76
232SERSERGLYGLY(chain I and (resid 439 through 459 or resid 466 through 497 or resid 506 through 545))IH506 - 54585 - 124
312ARGARGGLUGLU(chain J and (resid 439 through 459 or resid 466 through 497 or resid 506 through 545))JC439 - 45918 - 38
322ILEILEGLNGLN(chain J and (resid 439 through 459 or resid 466 through 497 or resid 506 through 545))JC466 - 49745 - 76
332SERSERGLYGLY(chain J and (resid 439 through 459 or resid 466 through 497 or resid 506 through 545))JC506 - 54585 - 124

NCS ensembles :
ID
1
2

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Components

#1: Protein
CYtoKinesis defect / CYK-4


Mass: 14494.723 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Caenorhabditis elegans (invertebrata) / Gene: cyk-4, CELE_K08E3.6, K08E3.6 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9XUS9
#2: Protein
Kinesin-like protein / ZEN-4


Mass: 15438.277 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Caenorhabditis elegans (invertebrata) / Gene: zen-4, CELE_M03D4.1, M03D4.1 / Production host: Escherichia coli (E. coli) / References: UniProt: G5EG83
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 38 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.57 Å3/Da / Density % sol: 52.08 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop
Details: 8-10% PEG3350, 100mM NaAc 5.0, 100mM tacsimate 5.0, 5mM DTT

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.97915 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 17, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97915 Å / Relative weight: 1
ReflectionResolution: 2.5→50 Å / Num. obs: 39530 / % possible obs: 95.95 % / Redundancy: 3.7 % / CC1/2: 0.829 / Net I/σ(I): 21.076
Reflection shellResolution: 2.5→2.57 Å / Num. unique obs: 3953 / CC1/2: 0.829

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Processing

Software
NameVersionClassification
PHENIX1.13_2998refinement
PDB_EXTRACT3.27data extraction
HKL-2000data reduction
HKL-2000data scaling
SHARPphasing
RefinementMethod to determine structure: SIRAS / Resolution: 2.5→41.653 Å / SU ML: 0.39 / Cross valid method: THROUGHOUT / σ(F): 1.98 / Phase error: 32.82 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2774 1636 4.14 %
Rwork0.2381 37879 -
obs0.2397 39515 95.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 197.25 Å2 / Biso mean: 83.5588 Å2 / Biso min: 30.89 Å2
Refinement stepCycle: final / Resolution: 2.5→41.653 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6672 0 0 38 6710
Biso mean---66.14 -
Num. residues----810
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11B2007X-RAY DIFFRACTION12.658TORSIONAL
12C2007X-RAY DIFFRACTION12.658TORSIONAL
13D2007X-RAY DIFFRACTION12.658TORSIONAL
14G2007X-RAY DIFFRACTION12.658TORSIONAL
21F1365X-RAY DIFFRACTION12.658TORSIONAL
22I1365X-RAY DIFFRACTION12.658TORSIONAL
23J1365X-RAY DIFFRACTION12.658TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.5-2.57360.37891340.3604317396
2.5736-2.65660.39881330.3328318597
2.6566-2.75160.33161440.3191319997
2.7516-2.86170.31161380.2763322598
2.8617-2.99190.32531350.2755320397
2.9919-3.14960.32991390.27320697
3.1496-3.34690.27821370.2492318698
3.3469-3.60510.31071400.2395323897
3.6051-3.96770.2291340.2063319697
3.9677-4.54120.24211340.1915313696
4.5412-5.71910.26141360.2173308793
5.7191-41.6530.25761320.2324284587
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.7517-1.33233.3248.6143-0.19565.67710.1781-2.0234-0.14280.0994-0.19482.02080.6376-1.7606-0.09970.5726-0.0544-0.04640.99630.12940.6903111.1905168.652658.0494
22.06330.86350.37392.05852.57622.11930.2394-0.2317-2.0017-1.5072-0.17731.60721.5232-2.52460.13650.8811-0.3247-0.16070.91570.03150.8791106.735159.852848.5451
34.5762-0.61741.03284.30212.59122.80141.3068-1.2010.15812.3934-1.61670.6378-0.29250.34650.24831.566-0.34970.07010.7806-0.17430.7378102.3053137.734839.7523
42.46562.98130.87359.50112.65882.3526-0.1244-0.0615-1.10790.649-0.00330.2959-0.28840.13840.18070.8299-0.05140.05150.6241-0.01720.898395.4385114.946726.9981
54.45422.3491.30582.6822.14871.6130.0360.11910.02690.33390.2943-0.775-0.31950.2407-0.34430.63350.15010.1730.559-0.03920.641279.338484.190717.2603
66.62961.7422-1.98738.82790.9394.68680.74240.81680.36080.5557-0.04410.3773-0.0372-1.5957-0.58450.48780.0575-0.07520.88870.11560.495562.720756.292417.2428
77.41971.8543-0.31465.5429-0.38716.669-0.92411.0554-0.24560.00880.8243-0.2167-0.1594-0.1677-0.07860.58830.0780.00430.59970.05110.3937112.9851171.496339.6477
82.6220.91591.36665.57160.94713.3794-0.1052-0.15610.65251.4146-0.28512.1749-0.0085-0.0020.4290.6802-0.07280.36340.4633-0.0780.946993.3228137.296233.5323
92.26125.4548-0.32232.06811.27084.26870.3188-0.07550.9419-0.3442-0.67230.5085-0.90560.39270.53641.0428-0.05280.23330.653-0.03481.189185.1777111.086229.9811
102.15841.81810.37632.26441.16421.58520.311-0.15180.3961.9429-0.54630.4713-0.0282-0.0530.40960.9522-0.06880.00260.53060.0210.854882.429385.16726.7761
112.99362.32941.46372.35014.21156.08860.15860.56240.55410.36050.5847-0.58980.15590.3667-0.86090.66040.0745-0.19490.4210.07220.81175.4260.202917.6465
120.32110.0329-0.97312.01432.68834.92350.84680.1868-1.4366-0.59661.8771.81162.3912-2.7267-0.97651.1804-0.2505-0.77150.81580.07371.295265.879343.340310.6737
136.7639-0.76441.64224.2863-1.6998.24920.84160.6074-0.4218-0.4962-0.2005-0.21630.55561.5454-0.66010.60550.0738-0.07380.7733-0.04690.487959.031958.17671.5827
144.64790.428-1.75614.18981.55886.2138-0.3458-0.2411-0.5558-0.13990.106-0.22990.39920.1321-0.03770.43140.02190.15980.45540.07240.426769.542890.810415.6854
151.25181.8569-0.66919.33181.21980.02130.4288-0.66710.66130.345-0.3726-0.3217-0.32740.3495-0.05540.4505-0.0680.12740.5932-0.09450.765677.0053117.189520.6518
167.40641.89540.7912.13231.61536.52850.47650.3219-0.94590.3966-0.67952.38350.8298-1.26571.0080.7344-0.0349-0.00750.6184-0.04281.649881.5797141.081520.9331
173.02486.01530.75142.1381.33840.1804-0.31840.6704-0.2090.24460.7852.03380.0702-0.586-0.48170.7138-0.0696-0.19420.61570.06991.335991.2112160.392120.9333
184.79385.35381.59819.30583.27222.6250.0027-0.14690.60320.3428-0.2122-0.4284-0.42350.14190.27580.86520.0305-0.29190.38510.07860.8427105.6765181.370126.7575
197.52173.54440.55017.02562.60842.29920.5001-0.3412-0.79390.59040.4899-0.43880.6883-0.927-0.76940.6636-0.1057-0.18620.66060.13340.5301122.3549167.076858.9062
201.581-0.9113-0.15690.53430.09730.2611-0.3542-1.5003-0.3845-0.3503-0.0746-0.70630.6794-0.13030.86631.4632-0.20720.46191.15860.08891.422689.7353133.500643.1257
210.68690.14180.56510.2009-0.1870.1688-0.06060.44780.65040.1355-0.46731.99790.37-0.22650.3451.1198-0.19870.42031.1868-0.30930.813594.7915153.75744.3034
227.85013.59223.88596.28122.29684.12210.3404-0.5491.05661.0535-0.60880.2245-0.4792-0.39310.16150.70810.0905-0.12970.5424-0.07380.5102113.7398180.58349.7613
238.51612.84960.80659.16671.18146.69780.46580.73541.25040.1343-0.3022-0.6714-1.76920.83870.09731.3348-0.0864-0.36370.6792-0.00041.1856129.6822194.263256.2651
242.27871.0186-0.72738.0138-7.5598.8521-0.50580.845-0.0146-0.22981.8673-0.6775-1.17951.3234-1.00141.7855-0.3638-0.63371.0378-0.23221.7833136.4562201.960261.8168
2510.08612.7095-0.9016.06421.81392.87561.23910.2775-1.49430.4241-0.3153-0.4541.26410.2839-0.9920.9089-0.0514-0.42190.94780.1340.814858.299851.53177.3091
263.21372.21170.6195.56511.81461.968-0.18910.30670.1064-0.62440.4317-0.5691-0.15230.5549-0.31660.5785-0.0660.19520.6742-0.01390.590678.575695.75228.434
274.86634.32042.24015.05151.98440.39530.03461.0067-0.50440.32590.47-0.3434-0.40980.4191-0.20390.8282-0.08790.00090.4841-0.00530.577664.491479.86540.1273
287.01192.45482.31863.21331.83132.10550.5074-0.0197-0.3198-0.0522-0.0443-0.21670.6269-0.3828-0.38710.58740.0044-0.17140.6347-0.03180.445340.257156.3414-5.2463
292.01246.6403-2.4368.2384-0.36131.781.1597-1.52331.71970.1056-0.30181.09640.8848-0.65380.0181.1519-0.3977-0.35291.03890.28610.697724.95241.0893-6.9604
302.62040.32830.16870.5047-0.5841.42530.70430.691-0.9598-0.8441-0.08732.16071.7272-1.4296-0.4661.5445-0.4858-0.50211.34080.17431.347317.172730.9378-9.5009
313.94752.69850.68885.51850.72212.96730.7857-0.36510.64990.9602-0.37330.1757-0.3357-0.0142-0.38740.63380.0415-0.05130.7647-0.34630.840247.557871.56144.4418
324.26311.9755-0.95686.54170.30923.3562-0.1060.39640.3541-1.194-0.17190.93940.4928-0.1740.23960.6489-0.0557-0.00560.41650.05570.465964.173894.73617.9821
334.51026.30020.92619.41952.860.4287-0.43110.25380.1881-1.51140.4119-1.1105-0.76550.6340.15570.5887-0.16210.13880.58990.04430.71978.8654114.583811.2046
347.80226.38155.30799.4413.97656.06780.58860.40510.44660.9456-0.94442.00140.2453-0.11240.51610.5094-0.02040.03230.516-0.0130.959490.5445135.974118.7566
352.83093.74692.0072.23320.55270.80940.03840.4571-0.07890.4674-0.22441.17020.0203-0.5357-0.02610.5816-0.03850.10910.6128-0.08970.761896.9885159.354428.2341
364.20250.0007-0.67687.70831.30464.24730.01810.07320.78460.6546-0.0092-0.8421-0.8133-0.6084-0.15740.74850.1567-0.18750.5820.00420.895101.1734186.849834.5253
371.8848-0.76621.02550.3496-0.42940.61031.58810.33981.08261.07211.1667-0.9267-0.5995-0.2396-1.53091.42730.4788-0.46320.6879-0.16771.4894108.7466190.881349.6078
383.0834-1.0518-0.725.30434.04139.5661-0.73420.02320.499-0.18780.03670.6379-0.65360.01340.74780.90410.0942-0.28330.5451-0.04030.6335109.8055180.979738.4493
392.22840.996-0.54367.2352-0.6071.64480.11570.4137-0.38910.2226-0.0826-0.5498-0.00110.13940.04630.60510.0051-0.04450.6284-0.05770.6491104.1478141.065927.6257
403.6231-1.4014-0.48382.02592.20699.3236-0.45410.7249-0.88080.36832.19182.2029-1.59470.5298-2.02831.2462-0.2046-0.14411.08890.00041.4653112.4656152.091839.2746
413.17013.78642.89526.44413.43364.5503-0.3897-0.33430.2673-0.8111-0.06480.3027-0.8285-0.19560.23320.54320.0109-0.17710.5110.03380.4617120.8075175.604454.9868
420.64381.95611.02464.95112.90481.7321-1.204-0.6592.4724-0.12720.7442-0.2136-0.0329-0.05080.24571.48060.0647-0.55480.8837-0.38571.8353129.6195199.755668.7822
435.32591.21331.13614.71071.13325.8887-0.1622-0.32840.35520.32190.203-0.1533-0.9825-0.289-0.22140.74410.0783-0.20860.5005-0.09960.677442.571170.3172-5.1101
445.58530.2428-1.9854.9567-1.30666.14420.32870.62120.3356-0.4577-0.03661.2940.6246-0.6629-0.49360.5783-0.0153-0.14790.4393-0.01420.656758.762399.971514.7804
454.11280.85340.09459.07350.09623.44630.13870.19340.24520.7347-0.420.07070.1038-0.32750.33310.39790.03630.10750.40150.01230.380861.404694.122120.5001
464.40362.15893.84544.2872.38765.43820.6057-0.97510.59091.2677-1.06770.45140.7474-1.08160.34950.4751-0.11490.02230.7888-0.0480.41852.926963.535310.5866
477.01537.16354.47018.00714.7542.8247-0.20050.1994-0.30030.75290.1753-0.5262-0.41980.08270.050.8699-0.0678-0.38780.88270.15270.877441.440245.2136-3.825
484.2171.9903-4.03482.0765-4.32256.41261.0362-0.5048-0.92360.71210.36520.90752.06940.0774-1.66661.4757-0.3813-0.37660.9080.02531.085126.902431.5876-13.8508
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain B and resid 9:20)B9 - 20
2X-RAY DIFFRACTION2(chain B and resid 21:27)B21 - 27
3X-RAY DIFFRACTION3(chain B and resid 28:46)B28 - 46
4X-RAY DIFFRACTION4(chain B and resid 47:64)B47 - 64
5X-RAY DIFFRACTION5(chain B and resid 65:92)B65 - 92
6X-RAY DIFFRACTION6(chain B and resid 93:109)B93 - 109
7X-RAY DIFFRACTION7(chain C and resid 9:27)C9 - 27
8X-RAY DIFFRACTION8(chain C and resid 28:52)C28 - 52
9X-RAY DIFFRACTION9(chain C and resid 53:65)C53 - 65
10X-RAY DIFFRACTION10(chain C and resid 66:87)C66 - 87
11X-RAY DIFFRACTION11(chain C and resid 88:102)C88 - 102
12X-RAY DIFFRACTION12(chain C and resid 103:114)C103 - 114
13X-RAY DIFFRACTION13(chain D and resid 9:24)D9 - 24
14X-RAY DIFFRACTION14(chain D and resid 28:45)D28 - 45
15X-RAY DIFFRACTION15(chain D and resid 46:65)D46 - 65
16X-RAY DIFFRACTION16(chain D and resid 66:77)D66 - 77
17X-RAY DIFFRACTION17(chain D and resid 78:93)D78 - 93
18X-RAY DIFFRACTION18(chain D and resid 94:112)D94 - 112
19X-RAY DIFFRACTION19(chain E and resid 438:456)E438 - 456
20X-RAY DIFFRACTION20(chain E and resid 457:487)E457 - 487
21X-RAY DIFFRACTION21(chain E and resid 488:509)E488 - 509
22X-RAY DIFFRACTION22(chain E and resid 510:527)E510 - 527
23X-RAY DIFFRACTION23(chain E and resid 528:538)E528 - 538
24X-RAY DIFFRACTION24(chain E and resid 539:543)E539 - 543
25X-RAY DIFFRACTION25(chain F and resid 443:458)F443 - 458
26X-RAY DIFFRACTION26(chain F and resid 465:487)F465 - 487
27X-RAY DIFFRACTION27(chain F and resid 488:512)F488 - 512
28X-RAY DIFFRACTION28(chain F and resid 513:533)F513 - 533
29X-RAY DIFFRACTION29(chain F and resid 534:540)F534 - 540
30X-RAY DIFFRACTION30(chain F and resid 541:550)F541 - 550
31X-RAY DIFFRACTION31(chain G and resid 9:29)G9 - 29
32X-RAY DIFFRACTION32(chain G and resid 30:43)G30 - 43
33X-RAY DIFFRACTION33(chain G and resid 44:63)G44 - 63
34X-RAY DIFFRACTION34(chain G and resid 64:77)G64 - 77
35X-RAY DIFFRACTION35(chain G and resid 78:97)G78 - 97
36X-RAY DIFFRACTION36(chain G and resid 98:115)G98 - 115
37X-RAY DIFFRACTION37(chain I and resid 439:443)I439 - 443
38X-RAY DIFFRACTION38(chain I and resid 444:458)I444 - 458
39X-RAY DIFFRACTION39(chain I and resid 459:493)I459 - 493
40X-RAY DIFFRACTION40(chain I and resid 494:506)I494 - 506
41X-RAY DIFFRACTION41(chain I and resid 507:533)I507 - 533
42X-RAY DIFFRACTION42(chain I and resid 534:545)I534 - 545
43X-RAY DIFFRACTION43(chain J and resid 439:460)J439 - 460
44X-RAY DIFFRACTION44(chain J and resid 461:478)J461 - 478
45X-RAY DIFFRACTION45(chain J and resid 479:500)J479 - 500
46X-RAY DIFFRACTION46(chain J and resid 501:517)J501 - 517
47X-RAY DIFFRACTION47(chain J and resid 518:536)J518 - 536
48X-RAY DIFFRACTION48(chain J and resid 537:547)J537 - 547

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