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- PDB-7e3x: Crystal structure of SDR family NAD(P)-dependent oxidoreductase f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7e3x | ||||||
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Title | Crystal structure of SDR family NAD(P)-dependent oxidoreductase from exiguobacterium | ||||||
![]() | Oxidoreductase | ||||||
![]() | OXIDOREDUCTASE / mutant / short chain reductase | ||||||
Function / homology | short chain dehydrogenase / Short-chain dehydrogenase/reductase, conserved site / Short-chain dehydrogenases/reductases family signature. / Short-chain dehydrogenase/reductase SDR / oxidoreductase activity / NAD(P)-binding domain superfamily / NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Oxidoreductase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chen, L. / Tang, J. / Yuan, S. / Zhang, F. / Chen, S. | ||||||
![]() | ![]() Title: Structure-guided evolution of a ketoreductase forefficient and stereoselective bioreduction of bulkyalpha-aminobeta-keto esters Authors: Tang, J. / Chen, L. / Zhang, L. / Ni, G. / Yu, J. / Wang, H. / Zhang, F. / Yuan, S. / Feng, M. / Che, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 110.8 KB | Display | ![]() |
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PDB format | ![]() | 83.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 19.8 KB | Display | |
Data in CIF | ![]() | 26.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7e24C ![]() 7e28C ![]() 5t2uS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28159.857 Da / Num. of mol.: 2 / Mutation: F88V, V127I, A138L, R142M, A190V, S193A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.62 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / Details: PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 130 mm / Detector: CCD / Date: Dec 25, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.58→50 Å / Num. obs: 17891 / % possible obs: 97.7 % / Redundancy: 4.8 % / Biso Wilson estimate: 29.57 Å2 / CC1/2: 0.948 / Net I/σ(I): 1.6 |
Reflection shell | Resolution: 2.6→2.64 Å / Mean I/σ(I) obs: 1.4 / Num. unique obs: 821 / CC1/2: 0.57 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5t2u Resolution: 2.58→44.76 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso max: 71.02 Å2 / Biso mean: 28.0926 Å2 / Biso min: 12.48 Å2 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.58→44.76 Å
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LS refinement shell | Resolution: 2.58→2.6693 Å
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