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データを開く
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基本情報
| 登録情報 | データベース: PDB / ID: 7dgr | |||||||||||||||
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| タイトル | Activity optimized supercomplex state2 | |||||||||||||||
要素 |
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キーワード | OXIDOREDUCTASE / Complex I / respiratory / electron transport | |||||||||||||||
| 機能・相同性 | 機能・相同性情報Complex III assembly / Complex I biogenesis / Complex IV assembly / TP53 Regulates Metabolic Genes / Mitochondrial protein import / RHOG GTPase cycle / respiratory chain complex IV assembly / Cytoprotection by HMOX1 / subthalamus development / pons development ...Complex III assembly / Complex I biogenesis / Complex IV assembly / TP53 Regulates Metabolic Genes / Mitochondrial protein import / RHOG GTPase cycle / respiratory chain complex IV assembly / Cytoprotection by HMOX1 / subthalamus development / pons development / mitochondrial respirasome assembly / cerebellar Purkinje cell layer development / Respiratory electron transport / pyramidal neuron development / respiratory chain complex IV / thalamus development / cellular response to oxygen levels / ubiquinone biosynthetic process / mitochondrial large ribosomal subunit binding / respiratory chain complex / gliogenesis / Mitochondrial translation termination / cytochrome-c oxidase / respiratory chain complex III / oxidative phosphorylation / quinol-cytochrome-c reductase / mitochondrial electron transport, cytochrome c to oxygen / Neutrophil degranulation / neural precursor cell proliferation / oxygen sensor activity / [2Fe-2S] cluster assembly / quinol-cytochrome-c reductase activity / cytochrome-c oxidase activity / mitochondrial electron transport, ubiquinol to cytochrome c / Mitochondrial protein degradation / hypothalamus development / midbrain development / NADH:ubiquinone reductase (H+-translocating) / ubiquinone binding / mitochondrial ATP synthesis coupled electron transport / electron transport coupled proton transport / mitochondrial electron transport, NADH to ubiquinone / acyl binding / mitochondrial respiratory chain complex I assembly / NADH dehydrogenase activity / oxidoreductase activity, acting on NAD(P)H / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / acyl carrier activity / response to cAMP / quinone binding / ATP synthesis coupled electron transport / enzyme regulator activity / reactive oxygen species metabolic process / neurogenesis / proton transmembrane transport / aerobic respiration / fatty acid binding / central nervous system development / respiratory electron transport chain / hippocampus development / electron transport chain / circadian rhythm / metalloendopeptidase activity / brain development / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / mitochondrial membrane / NAD binding / fatty acid biosynthetic process / FMN binding / 4 iron, 4 sulfur cluster binding / oxidoreductase activity / mitochondrial inner membrane / mitochondrial matrix / copper ion binding / negative regulation of DNA-templated transcription / apoptotic process / heme binding / protein-containing complex binding / protein-containing complex / mitochondrion / proteolysis / nucleoplasm / membrane / metal ion binding / cytoplasm 類似検索 - 分子機能 | |||||||||||||||
| 生物種 | ![]() | |||||||||||||||
| 手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.6 Å | |||||||||||||||
データ登録者 | Jeon, T.J. / Lee, S.G. / Yoo, S.H. / Ryu, J.H. / Kim, D.S. / Hyun, J.K. / Kim, H.M. / Ryu, S.E. | |||||||||||||||
引用 | ジャーナル: Antioxid Redox Signal / 年: 2022タイトル: A Dynamic Substrate Pool Revealed by cryo-EM of a Lipid-Preserved Respiratory Supercomplex. 著者: Tae Jin Jeon / Seong-Gyu Lee / Suk Hyun Yoo / Myeongbin Kim / Dabin Song / Joonghyun Ryu / Hwangseo Park / Deok-Soo Kim / Jaekyung Hyun / Ho Min Kim / Seong Eon Ryu / ![]() 要旨: Mitochondrial respiratory supercomplexes mediate redox electron transfer, generating a proton gradient for ATP synthesis. To provide structural information on the function of supercomplexes in ... Mitochondrial respiratory supercomplexes mediate redox electron transfer, generating a proton gradient for ATP synthesis. To provide structural information on the function of supercomplexes in physiologically relevant conditions, we conducted cryoelectron microscopy studies with supercomplexes in a lipid-preserving state. Here, we present cryoelectron microscopy structures of bovine respiratory supercomplex IIIIIV by using a lipid-preserving sample preparation. The preparation greatly enhances the intercomplex quinone transfer activity. The structures reveal large intercomplex motions that result in different shapes and sizes of the intercomplex space between complexes I and III, forming a dynamic substrate pool. Biochemical and structural analyses indicated that intercomplex phospholipids mediate the intercomplex motions. An analysis of the different classes of focus-refined complex I showed that structural switches due to quinone reduction led to the formation of a novel channel that could transfer reduced quinones to the intercomplex substrate pool. Our results indicate potential mechanism for the facilitated electron transfer involving a dynamic substrate pool and intercomplex movement by which supercomplexes play an active role in the regulation of metabolic flux and reactive oxygen species. | |||||||||||||||
| 履歴 |
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構造の表示
| 構造ビューア | 分子: Molmil Jmol/JSmol |
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ダウンロードとリンク
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ダウンロード
| PDBx/mmCIF形式 | 7dgr.cif.gz | 2.5 MB | 表示 | PDBx/mmCIF形式 |
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| PDB形式 | pdb7dgr.ent.gz | 表示 | PDB形式 | |
| PDBx/mmJSON形式 | 7dgr.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
| その他 | その他のダウンロード |
-検証レポート
| アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/dg/7dgr ftp://data.pdbj.org/pub/pdb/validation_reports/dg/7dgr | HTTPS FTP |
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-関連構造データ
| 関連構造データ | ![]() 30674MC ![]() 7dgqC ![]() 7dgsC ![]() 7dgzC ![]() 7dh0C ![]() 7dkfC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
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集合体
| 登録構造単位 | ![]()
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| 1 |
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要素
+NADH dehydrogenase [ubiquinone] flavoprotein ... , 3種, 3分子 98F
+NADH-ubiquinone oxidoreductase chain ... , 7種, 7分子 4276531
+タンパク質 , 4種, 7分子 AWMmyoz
+NADH dehydrogenase [ubiquinone] iron-sulfur protein ... , 7種, 7分子 BCDEGHI
+NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit ... , 12種, 12分子 JKLNOPQRSTUV
+NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit ... , 11種, 11分子 XYZabcdfhge
+NADH dehydrogenase [ubiquinone] 1 subunit ... , 2種, 2分子 ij
+Cytochrome b-c1 complex subunit ... , 9種, 18分子 kwlxpA0qA1rA2sB3tA3uB2vB1
+Cytochrome c oxidase subunit ... , 13種, 13分子 A9C4C2A7C0A6A4A5B4C3B0C1A8
+非ポリマー , 13種, 37分子 
























+詳細
-実験情報
-実験
| 実験 | 手法: 電子顕微鏡法 |
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| EM実験 | 試料の集合状態: TISSUE / 3次元再構成法: 単粒子再構成法 |
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試料調製
| 構成要素 | 名称: NADH-UBIQUINONE OXIDOREDUCTASE / タイプ: COMPLEX / Entity ID: #1-#68 / 由来: NATURAL |
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| 由来(天然) | 生物種: ![]() |
| 緩衝液 | pH: 7.4 |
| 試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
| 急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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| 顕微鏡 | モデル: FEI TITAN KRIOS |
| 電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
| 電子レンズ | モード: BRIGHT FIELD |
| 撮影 | 電子線照射量: 35 e/Å2 フィルム・検出器のモデル: FEI FALCON II (4k x 4k) |
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解析
| CTF補正 | タイプ: NONE |
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| 対称性 | 点対称性: C1 (非対称) |
| 3次元再構成 | 解像度: 4.6 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 57806 / 対称性のタイプ: POINT |
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FIELD EMISSION GUN