National Natural Science Foundation of China (NSFC)
31570736
中国
引用
ジャーナル: PLoS Pathog / 年: 2021 タイトル: Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission. 著者: Qianqian Shao / Xudong Jia / Yuanzhu Gao / Zhe Liu / Huan Zhang / Qiqi Tan / Xin Zhang / Huiqiong Zhou / Yinyin Li / De Wu / Qinfen Zhang / 要旨: Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like ...Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like viruses, with features similar to those of the Totiviridae family. Most reported members of this family infect fungi or protozoans and lack an extracellular life cycle stage. Here, we identified a new strain of OmRV and determined high-resolution structures for this virus using single-particle cryo-electron microscopy. The structures feature an unexpected protrusion at the five-fold vertex of the capsid. Disassociation of the protrusion could result in several conformational changes in the major capsid. All these structures, together with some biological results, suggest the protrusions' associations with the extracellular transmission of OmRV.
モード: BRIGHT FIELD / 倍率(公称値): 75000 X / 最大 デフォーカス(公称値): 2500 nm / 最小 デフォーカス(公称値): 1000 nm / アライメント法: NONE
試料ホルダ
凍結剤: NITROGEN
撮影
電子線照射量: 39 e/Å2 / 検出モード: COUNTING フィルム・検出器のモデル: FEI FALCON III (4k x 4k)
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解析
EMソフトウェア
ID
名称
バージョン
カテゴリ
2
ETHAN
粒子像選択
3
EPU
画像取得
5
Gctf
CTF補正
10
PHENIX
1.17
モデル精密化
11
RELION
初期オイラー角割当
12
jspr
最終オイラー角割当
13
RELION
3.1
分類
14
RELION
3.1
3次元再構成
CTF補正
タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
粒子像の選択
選択した粒子像数: 1160016 詳細: Subtracted from every five-fold vertexes from 96668 virus particles. Each virus contains 12 five-fold vertexes. 96668x12=1160016.
対称性
点対称性: C5 (5回回転対称)
3次元再構成
解像度: 4.1 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 38935 / 対称性のタイプ: POINT