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- PDB-7cq0: Crystal structure of Streptoavidin-C1 from Streptomyces cinamonensis -
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Open data
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Basic information
Entry | Database: PDB / ID: 7cq0 | ||||||
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Title | Crystal structure of Streptoavidin-C1 from Streptomyces cinamonensis | ||||||
![]() | Mature Streptoavidin-C1 | ||||||
![]() | ANTIMICROBIAL PROTEIN / Avidin / Antifungal / Biotin | ||||||
Function / homology | Avidin / Avidin/streptavidin / Avidin-like superfamily / Avidin family / Avidin-like domain profile. / biotin binding / extracellular region / Avidin![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jeon, B.J. / Kim, S. / Lee, J.-H. / Kim, M.S. / Hwang, K.Y. | ||||||
![]() | ![]() Title: Insights into the structure of mature streptavidin C1 from Streptomyces cinnamonensis reveal the self-binding of the extension C-terminal peptide to biotin-binding sites. Authors: Jeon, B.J. / Kim, S. / Kim, M.S. / Lee, J.H. / Kim, B.S. / Hwang, K.Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.2 KB | Display | ![]() |
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PDB format | ![]() | 28.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7cpzC ![]() 6hdsS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 20172.203 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: A0A0M8UVL7 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.66 Å3/Da / Density % sol: 25.96 % |
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Crystal grow | Temperature: 298.15 K / Method: batch mode / Details: PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 R CdTe 300K / Detector: PIXEL / Date: May 8, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→46.88 Å / Num. obs: 16769 / % possible obs: 99.8 % / Redundancy: 20 % / Biso Wilson estimate: 24.97 Å2 / Rrim(I) all: 0.315 / Net I/σ(I): 6.3 |
Reflection shell | Resolution: 2.03→2.09 Å / Num. unique obs: 16769 / Rrim(I) all: 0.315 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6hds Resolution: 2.03→46.88 Å / SU ML: 0.21 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 24.1929 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.64 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.03→46.88 Å
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Refine LS restraints |
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LS refinement shell |
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