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Open data
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Basic information
Entry | Database: PDB / ID: 7c46 | ||||||
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Title | Crystal Structure of Rv1045 toxin point variant K189A | ||||||
![]() | Rv1045 toxin | ||||||
![]() | TRANSFERASE / Nucleotidyltransferase / Toxin-Antitoxin / TglT Toxin / Rv1045 / guanylyltransferase | ||||||
Function / homology | Nucleotidyl transferase AbiEii toxin, Type IV TA system / Nucleotidyl transferase AbiEii toxin, Type IV TA system / metal ion binding / Nucleotidyl transferase AbiEii/AbiGii toxin family protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Deep, A. / Kaur, J. / Thakur, K.G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal Structure of Rv1045 toxin point variant K189A Authors: Deep, A. / Kaur, J. / Thakur, K.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 132.9 KB | Display | ![]() |
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PDB format | ![]() | 107.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 32127.082 Da / Num. of mol.: 1 / Mutation: K189A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: Rv1045 / Production host: ![]() ![]() | ||||
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#2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.14 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 / Details: 2.4M ammonium phosphate dibasic, 0.1M Tris pH 8.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 9, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97947 Å / Relative weight: 1 |
Reflection | Resolution: 1.93→81.41 Å / Num. obs: 23924 / % possible obs: 100 % / Redundancy: 11.7 % / CC1/2: 0.998 / Rmerge(I) obs: 0.132 / Rpim(I) all: 0.04 / Rrim(I) all: 0.138 / Net I/σ(I): 16.2 |
Reflection shell | Resolution: 1.93→2 Å / Redundancy: 11.2 % / Rmerge(I) obs: 1.029 / Num. unique obs: 2386 / CC1/2: 0.768 / Rpim(I) all: 0.318 / Rrim(I) all: 1.078 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.74 Å2
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Refinement step | Cycle: 1 / Resolution: 1.93→81.41 Å
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Refine LS restraints |
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