+Open data
-Basic information
Entry | Database: PDB / ID: 7bvl | ||||||
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Title | Crystal structure of glucose isomerase delivered in wheat starch | ||||||
Components | Xylose isomerase | ||||||
Keywords | ISOMERASE / glucose isomerase | ||||||
Function / homology | Function and homology information xylose isomerase / D-xylose metabolic process / xylose isomerase activity / magnesium ion binding / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Streptomyces rubiginosus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Nam, K.H. | ||||||
Funding support | Korea, Republic Of, 1items
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Citation | Journal: Int J Mol Sci / Year: 2020 Title: Polysaccharide-Based Injection Matrix for Serial Crystallography. Authors: Nam, K.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7bvl.cif.gz | 95.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7bvl.ent.gz | 70.4 KB | Display | PDB format |
PDBx/mmJSON format | 7bvl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bv/7bvl ftp://data.pdbj.org/pub/pdb/validation_reports/bv/7bvl | HTTPS FTP |
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-Related structure data
Related structure data | 7bvmC 7bvnC 7bvoC 5zydS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | |
Experimental dataset #1 | Data reference: 10.11577/1631283 / Data set type: diffraction image data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 43283.297 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Streptomyces rubiginosus (bacteria) / References: UniProt: P24300, xylose isomerase | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.98 % |
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Crystal grow | Temperature: 293.5 K / Method: batch mode / pH: 7 / Details: Tris-HCl, Ammonium sulfate, Magnesium sulfate |
-Data collection
Diffraction | Mean temperature: 298.15 K / Serial crystal experiment: Y |
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 11C / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 9, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2→72.4 Å / Num. obs: 33248 / % possible obs: 100 % / Redundancy: 148.5 % / CC1/2: 0.9755 / CC star: 0.9937 / R split: 1.722E-6 / Net I/σ(I): 4.01 |
Reflection shell | Resolution: 2→2.07 Å / Num. unique obs: 3247 / CC1/2: 0.5966 / CC star: 0.8644 / R split: 6.564E-6 |
Serial crystallography sample delivery | Method: injection |
Serial crystallography sample delivery injection | Description: commercial syringe / Flow rate: 0.2 µL/min / Injector diameter: 168 µm / Injector temperature: 298.15 K |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5ZYD Resolution: 2→70.749 Å / SU ML: 0.28 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 22.17
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 86.71 Å2 / Biso mean: 30.0184 Å2 / Biso min: 14.87 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2→70.749 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / % reflection obs: 100 %
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