Mass: 13466.042 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC
1
2
1
isotropic
1
2D 1H-13C HSQC aliphatic
1
3
1
isotropic
1
2D 1H-13C HSQC aromatic
1
4
1
isotropic
3
3D HNCO
1
5
1
isotropic
3
3DHN(CA)CO
1
6
1
isotropic
1
3D HNCA
1
7
1
isotropic
1
3DHN(CO)CA
1
8
1
isotropic
1
3DCBCA(CO)NH
1
9
1
isotropic
1
3D HN(CA)CB
1
10
1
isotropic
1
3DC(CO)NH
1
11
1
isotropic
1
3DH(CCO)NH
1
12
1
isotropic
3
3D (H)N(CA)NH
1
13
1
isotropic
3
3DH(NCA)NH
1
16
1
isotropic
3
3DHBHA(CO)NH
1
17
1
isotropic
3
3DHNHAHB
1
14
1
isotropic
3
3D (H)CCH-TOCSY aliphatic
1
15
1
isotropic
1
3D (H)CCH-TOCSY aromatic
1
18
1
isotropic
3
3D 1H-15N NOESY
1
19
1
isotropic
3
3D 1H-13C NOESY aliphatic
1
20
1
isotropic
3
3D 1H-13C NOESY aromatic
1
21
1
isotropic
3
2D 1H-15N HSQCIPAP
1
22
2
anisotropic
3
2D 1H-15N HSQCIPAP
1
23
1
isotropic
3
3D J-HNCO
1
24
2
anisotropic
3
3D J-HNCO
1
25
1
isotropic
3
3D J-HN(CO)CA
1
26
2
anisotropic
3
3D J-HN(CO)CA
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Sample preparation
Details
Type
Solution-ID
Contents
Label
Solvent system
solution
1
0.8 mM [U-100% 13C; U-100% 15N] foldU5, 1.06 mM potassium phosphate, 2.97 mM sodium phosphate, 155.2 mM sodium chloride, 10 uM EDTA, 0.02 % sodium azide, 0.16 % w/w protease inhibitor complete cocktail, 95% H2O/5% D2O
Nomur
95% H2O/5% D2O
solution
2
0.4 mM [U-100% 13C; U-100% 15N] foldU5, 1.06 mM potassium phosphate, 2.97 mM sodium phosphate, 155.2 mM sodium chloride, 10 mM EDTA, 0.02 % sodium azide, 0.16 % w/w protease inhibitor complete cocktail, 10 mg/mL Pf1 phage, 95% H2O/5% D2O