+Open data
-Basic information
Entry | Database: PDB / ID: 7b7p | ||||||
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Title | PilB minor pilin from Streptococcus sanguinis | ||||||
Components | Type IV pilus biogenesis protein PilB | ||||||
Keywords | CELL ADHESION / type IV pili / pilin / von Willebrand factor A-like domain / MIDAS | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Streptococcus sanguinis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.26 Å | ||||||
Authors | Pelicic, V. / Sheppard, D. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2021 Title: PilB from Streptococcus sanguinis is a bimodular type IV pilin with a direct role in adhesion. Authors: Raynaud, C. / Sheppard, D. / Berry, J.L. / Gurung, I. / Pelicic, V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7b7p.cif.gz | 172.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7b7p.ent.gz | 140.4 KB | Display | PDB format |
PDBx/mmJSON format | 7b7p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b7/7b7p ftp://data.pdbj.org/pub/pdb/validation_reports/b7/7b7p | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 47454.379 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus sanguinis (bacteria) / Gene: pilB, SSV_2238 / Plasmid: pET28b / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0B7GP99 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.31 Å3/Da / Density % sol: 62.85 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.1 M Bis-tris propane (pH 7.0), 3 M NaCl |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.976 Å |
Detector | Type: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Apr 7, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976 Å / Relative weight: 1 |
Reflection | Resolution: 2.26→56.9 Å / Num. obs: 57372 / % possible obs: 99.4 % / Redundancy: 5.7 % / CC1/2: 0.999 / Rmerge(I) obs: 0.062 / Rrim(I) all: 0.068 / Net I/σ(I): 13.4 |
Reflection shell | Resolution: 2.26→2.3 Å / Num. unique obs: 2784 / CC1/2: 0.244 / % possible all: 96.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: SelenoMet Resolution: 2.26→56.88 Å / Cross valid method: FREE R-VALUE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 80.57 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.26→56.88 Å
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Refine LS restraints |
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