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- PDB-7art: 48 helix bundle DNA origami brick -

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Basic information

Entry
Database: PDB / ID: 7art
Title48 helix bundle DNA origami brick
Components
  • (STAPLE STRAND) x 211
  • SCAFFOLD STRAND
KeywordsDNA / DNA Origami
Function / homologyDNA / DNA (> 10) / DNA (> 100) / DNA (> 1000)
Function and homology information
Biological speciessynthetic construct (others)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 10 Å
AuthorsFeigl, E. / Kube, M. / Kohler, F. / Nagel-Yuksel, B. / Willner, E.M. / Funke, J.J. / Gerling, T. / Stommer, P. / Honemann, M.N. / Martin, T.G. ...Feigl, E. / Kube, M. / Kohler, F. / Nagel-Yuksel, B. / Willner, E.M. / Funke, J.J. / Gerling, T. / Stommer, P. / Honemann, M.N. / Martin, T.G. / Scheres, S.H.W. / Dietz, H.
Funding support Germany, 2items
OrganizationGrant numberCountry
German Research Foundation (DFG) Germany
European Research Council (ERC) Germany
CitationJournal: Nat Commun / Year: 2020
Title: Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution.
Authors: Massimo Kube / Fabian Kohler / Elija Feigl / Baki Nagel-Yüksel / Elena M Willner / Jonas J Funke / Thomas Gerling / Pierre Stömmer / Maximilian N Honemann / Thomas G Martin / Sjors H W ...Authors: Massimo Kube / Fabian Kohler / Elija Feigl / Baki Nagel-Yüksel / Elena M Willner / Jonas J Funke / Thomas Gerling / Pierre Stömmer / Maximilian N Honemann / Thomas G Martin / Sjors H W Scheres / Hendrik Dietz /
Abstract: The methods of DNA nanotechnology enable the rational design of custom shapes that self-assemble in solution from sets of DNA molecules. DNA origami, in which a long template DNA single strand is ...The methods of DNA nanotechnology enable the rational design of custom shapes that self-assemble in solution from sets of DNA molecules. DNA origami, in which a long template DNA single strand is folded by many short DNA oligonucleotides, can be employed to make objects comprising hundreds of unique DNA strands and thousands of base pairs, thus in principle providing many degrees of freedom for modelling complex objects of defined 3D shapes and sizes. Here, we address the problem of accurate structural validation of DNA objects in solution with cryo-EM based methodologies. By taking into account structural fluctuations, we can determine structures with improved detail compared to previous work. To interpret the experimental cryo-EM maps, we present molecular-dynamics-based methods for building pseudo-atomic models in a semi-automated fashion. Among other features, our data allows discerning details such as helical grooves, single-strand versus double-strand crossovers, backbone phosphate positions, and single-strand breaks. Obtaining this higher level of detail is a step forward that now allows designers to inspect and refine their designs with base-pair level interventions.
History
DepositionOct 26, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 11, 2020Provider: repository / Type: Initial release
Revision 1.1Dec 23, 2020Group: Structure summary / Category: audit_author
Revision 1.2Dec 30, 2020Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.3May 15, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Assembly

Deposited unit
AA: SCAFFOLD STRAND
AB: STAPLE STRAND
AC: STAPLE STRAND
AD: STAPLE STRAND
AE: STAPLE STRAND
AF: STAPLE STRAND
AG: STAPLE STRAND
AH: STAPLE STRAND
AI: STAPLE STRAND
AJ: STAPLE STRAND
AK: STAPLE STRAND
AL: STAPLE STRAND
AM: STAPLE STRAND
AN: STAPLE STRAND
AO: STAPLE STRAND
AP: STAPLE STRAND
AQ: STAPLE STRAND
AR: STAPLE STRAND
AS: STAPLE STRAND
AT: STAPLE STRAND
AU: STAPLE STRAND
AV: STAPLE STRAND
AW: STAPLE STRAND
AX: STAPLE STRAND
AY: STAPLE STRAND
AZ: STAPLE STRAND
Aa: STAPLE STRAND
Ab: STAPLE STRAND
Ac: STAPLE STRAND
Ad: STAPLE STRAND
Ae: STAPLE STRAND
Af: STAPLE STRAND
Ag: STAPLE STRAND
Ah: STAPLE STRAND
Ai: STAPLE STRAND
Aj: STAPLE STRAND
Ak: STAPLE STRAND
Al: STAPLE STRAND
Am: STAPLE STRAND
An: STAPLE STRAND
Ao: STAPLE STRAND
Ap: STAPLE STRAND
Aq: STAPLE STRAND
Ar: STAPLE STRAND
As: STAPLE STRAND
At: STAPLE STRAND
Au: STAPLE STRAND
Av: STAPLE STRAND
Aw: STAPLE STRAND
Ax: STAPLE STRAND
Ay: STAPLE STRAND
Az: STAPLE STRAND
A0: STAPLE STRAND
A1: STAPLE STRAND
A2: STAPLE STRAND
A3: STAPLE STRAND
A4: STAPLE STRAND
A5: STAPLE STRAND
A6: STAPLE STRAND
A7: STAPLE STRAND
A8: STAPLE STRAND
A9: STAPLE STRAND
BA: STAPLE STRAND
BB: STAPLE STRAND
BC: STAPLE STRAND
BD: STAPLE STRAND
BE: STAPLE STRAND
BF: STAPLE STRAND
BG: STAPLE STRAND
BH: STAPLE STRAND
BI: STAPLE STRAND
BJ: STAPLE STRAND
BK: STAPLE STRAND
BL: STAPLE STRAND
BM: STAPLE STRAND
BN: STAPLE STRAND
BO: STAPLE STRAND
BP: STAPLE STRAND
BQ: STAPLE STRAND
BR: STAPLE STRAND
BS: STAPLE STRAND
BT: STAPLE STRAND
BU: STAPLE STRAND
BV: STAPLE STRAND
BW: STAPLE STRAND
BX: STAPLE STRAND
BY: STAPLE STRAND
BZ: STAPLE STRAND
Ba: STAPLE STRAND
Bb: STAPLE STRAND
Bc: STAPLE STRAND
Bd: STAPLE STRAND
Be: STAPLE STRAND
Bf: STAPLE STRAND
Bg: STAPLE STRAND
Bh: STAPLE STRAND
Bi: STAPLE STRAND
Bj: STAPLE STRAND
Bk: STAPLE STRAND
Bl: STAPLE STRAND
Bm: STAPLE STRAND
Bn: STAPLE STRAND
Bo: STAPLE STRAND
Bp: STAPLE STRAND
Bq: STAPLE STRAND
Br: STAPLE STRAND
Bs: STAPLE STRAND
Bt: STAPLE STRAND
Bu: STAPLE STRAND
Bv: STAPLE STRAND
Bw: STAPLE STRAND
Bx: STAPLE STRAND
By: STAPLE STRAND
Bz: STAPLE STRAND
B0: STAPLE STRAND
B1: STAPLE STRAND
B2: STAPLE STRAND
B3: STAPLE STRAND
B4: STAPLE STRAND
B5: STAPLE STRAND
B6: STAPLE STRAND
B7: STAPLE STRAND
B8: STAPLE STRAND
B9: STAPLE STRAND
CA: STAPLE STRAND
CB: STAPLE STRAND
CC: STAPLE STRAND
CD: STAPLE STRAND
CE: STAPLE STRAND
CF: STAPLE STRAND
CG: STAPLE STRAND
CH: STAPLE STRAND
CI: STAPLE STRAND
CJ: STAPLE STRAND
CK: STAPLE STRAND
CL: STAPLE STRAND
CM: STAPLE STRAND
CN: STAPLE STRAND
CO: STAPLE STRAND
CP: STAPLE STRAND
CQ: STAPLE STRAND
CR: STAPLE STRAND
CS: STAPLE STRAND
CT: STAPLE STRAND
CU: STAPLE STRAND
CV: STAPLE STRAND
CW: STAPLE STRAND
CX: STAPLE STRAND
CY: STAPLE STRAND
CZ: STAPLE STRAND
Ca: STAPLE STRAND
Cb: STAPLE STRAND
Cc: STAPLE STRAND
Cd: STAPLE STRAND
Ce: STAPLE STRAND
Cf: STAPLE STRAND
Cg: STAPLE STRAND
Ch: STAPLE STRAND
Ci: STAPLE STRAND
Cj: STAPLE STRAND
Ck: STAPLE STRAND
Cl: STAPLE STRAND
Cm: STAPLE STRAND
Cn: STAPLE STRAND
Co: STAPLE STRAND
Cp: STAPLE STRAND
Cq: STAPLE STRAND
Cr: STAPLE STRAND
Cs: STAPLE STRAND
Ct: STAPLE STRAND
Cu: STAPLE STRAND
Cv: STAPLE STRAND
Cw: STAPLE STRAND
Cx: STAPLE STRAND
Cy: STAPLE STRAND
Cz: STAPLE STRAND
C0: STAPLE STRAND
C1: STAPLE STRAND
C2: STAPLE STRAND
C3: STAPLE STRAND
C4: STAPLE STRAND
C5: STAPLE STRAND
C6: STAPLE STRAND
C7: STAPLE STRAND
C8: STAPLE STRAND
C9: STAPLE STRAND
DA: STAPLE STRAND
DB: STAPLE STRAND
DC: STAPLE STRAND
DD: STAPLE STRAND
DE: STAPLE STRAND
DF: STAPLE STRAND
DG: STAPLE STRAND
DH: STAPLE STRAND
DI: STAPLE STRAND
DJ: STAPLE STRAND
DK: STAPLE STRAND
DL: STAPLE STRAND
DM: STAPLE STRAND
DN: STAPLE STRAND
DO: STAPLE STRAND
DP: STAPLE STRAND
DQ: STAPLE STRAND
DR: STAPLE STRAND
DS: STAPLE STRAND
DT: STAPLE STRAND
DU: STAPLE STRAND
DV: STAPLE STRAND
DW: STAPLE STRAND
DX: STAPLE STRAND
DY: STAPLE STRAND
DZ: STAPLE STRAND


Theoretical massNumber of molelcules
Total (without water)5,263,661212
Polymers5,263,661212
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: native gel electrophoresis
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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DNA chain , 212 types, 212 molecules AAABACADAEAFAGAHAIAJAKALAMANAOAPAQARASATAUAVAWAXAYAZAaAbAcAd...

#1: DNA chain SCAFFOLD STRAND


Mass: 2488158.250 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#2: DNA chain STAPLE STRAND


Mass: 12272.905 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#3: DNA chain STAPLE STRAND


Mass: 13423.601 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#4: DNA chain STAPLE STRAND


Mass: 15547.976 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#5: DNA chain STAPLE STRAND


Mass: 14352.249 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#6: DNA chain STAPLE STRAND


Mass: 15169.768 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#7: DNA chain STAPLE STRAND


Mass: 15018.687 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#8: DNA chain STAPLE STRAND


Mass: 15235.794 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#9: DNA chain STAPLE STRAND


Mass: 15137.720 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#10: DNA chain STAPLE STRAND


Mass: 12953.351 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#11: DNA chain STAPLE STRAND


Mass: 16705.730 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#12: DNA chain STAPLE STRAND


Mass: 11624.460 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#13: DNA chain STAPLE STRAND


Mass: 13500.686 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#14: DNA chain STAPLE STRAND


Mass: 10739.949 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#15: DNA chain STAPLE STRAND


Mass: 12184.833 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#16: DNA chain STAPLE STRAND


Mass: 12854.285 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#17: DNA chain STAPLE STRAND


Mass: 10932.042 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#18: DNA chain STAPLE STRAND


Mass: 15240.839 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#19: DNA chain STAPLE STRAND


Mass: 12784.240 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#20: DNA chain STAPLE STRAND


Mass: 11658.540 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#21: DNA chain STAPLE STRAND


Mass: 14523.438 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#22: DNA chain STAPLE STRAND


Mass: 11335.298 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#23: DNA chain STAPLE STRAND


Mass: 16741.850 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#24: DNA chain STAPLE STRAND


Mass: 11039.095 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#25: DNA chain STAPLE STRAND


Mass: 11093.142 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#26: DNA chain STAPLE STRAND


Mass: 13354.612 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#27: DNA chain STAPLE STRAND


Mass: 11705.550 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#28: DNA chain STAPLE STRAND


Mass: 13086.493 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#29: DNA chain STAPLE STRAND


Mass: 15161.758 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#30: DNA chain STAPLE STRAND


Mass: 13106.407 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#31: DNA chain STAPLE STRAND


Mass: 12918.424 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#32: DNA chain STAPLE STRAND


Mass: 10899.003 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#33: DNA chain STAPLE STRAND


Mass: 12265.922 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#34: DNA chain STAPLE STRAND


Mass: 13019.429 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#35: DNA chain STAPLE STRAND


Mass: 12989.315 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#36: DNA chain STAPLE STRAND


Mass: 12760.215 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#37: DNA chain STAPLE STRAND


Mass: 12930.366 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#38: DNA chain STAPLE STRAND


Mass: 12883.304 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#39: DNA chain STAPLE STRAND


Mass: 13497.667 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#40: DNA chain STAPLE STRAND


Mass: 11592.462 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#41: DNA chain STAPLE STRAND


Mass: 15036.767 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#42: DNA chain STAPLE STRAND


Mass: 15136.819 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#43: DNA chain STAPLE STRAND


Mass: 14317.219 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#44: DNA chain STAPLE STRAND


Mass: 12817.280 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#45: DNA chain STAPLE STRAND


Mass: 12923.378 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#46: DNA chain STAPLE STRAND


Mass: 15828.127 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#47: DNA chain STAPLE STRAND


Mass: 15116.709 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#48: DNA chain STAPLE STRAND


Mass: 11434.362 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#49: DNA chain STAPLE STRAND


Mass: 13063.404 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#50: DNA chain STAPLE STRAND


Mass: 13359.549 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#51: DNA chain STAPLE STRAND


Mass: 12215.842 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#52: DNA chain STAPLE STRAND


Mass: 10930.029 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#53: DNA chain STAPLE STRAND


Mass: 19365.443 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#54: DNA chain STAPLE STRAND


Mass: 15000.661 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#55: DNA chain STAPLE STRAND


Mass: 12994.414 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#56: DNA chain STAPLE STRAND


Mass: 12291.919 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#57: DNA chain STAPLE STRAND


Mass: 13455.650 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#58: DNA chain STAPLE STRAND


Mass: 15422.948 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#59: DNA chain STAPLE STRAND


Mass: 15200.829 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#60: DNA chain STAPLE STRAND


Mass: 12812.271 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#61: DNA chain STAPLE STRAND


Mass: 15309.863 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#62: DNA chain STAPLE STRAND


Mass: 14996.654 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#63: DNA chain STAPLE STRAND


Mass: 15082.770 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#64: DNA chain STAPLE STRAND


Mass: 15186.811 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#65: DNA chain STAPLE STRAND


Mass: 15028.688 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#66: DNA chain STAPLE STRAND


Mass: 11648.539 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#67: DNA chain STAPLE STRAND


Mass: 13518.713 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#68: DNA chain STAPLE STRAND


Mass: 12971.431 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#69: DNA chain STAPLE STRAND


Mass: 13066.472 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#70: DNA chain STAPLE STRAND


Mass: 12272.961 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#71: DNA chain STAPLE STRAND


Mass: 13130.484 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#72: DNA chain STAPLE STRAND


Mass: 11547.392 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#73: DNA chain STAPLE STRAND


Mass: 10140.524 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#74: DNA chain STAPLE STRAND


Mass: 13121.486 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#75: DNA chain STAPLE STRAND


Mass: 13257.506 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#76: DNA chain STAPLE STRAND


Mass: 15768.205 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#77: DNA chain STAPLE STRAND


Mass: 10759.967 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#78: DNA chain STAPLE STRAND


Mass: 12258.971 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#79: DNA chain STAPLE STRAND


Mass: 11416.389 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#80: DNA chain STAPLE STRAND


Mass: 11673.498 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#81: DNA chain STAPLE STRAND


Mass: 14480.311 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#82: DNA chain STAPLE STRAND


Mass: 11514.465 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#83: DNA chain STAPLE STRAND


Mass: 12191.870 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#84: DNA chain STAPLE STRAND


Mass: 12936.399 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#85: DNA chain STAPLE STRAND


Mass: 10700.965 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#86: DNA chain STAPLE STRAND


Mass: 12914.366 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#87: DNA chain STAPLE STRAND


Mass: 10737.973 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#88: DNA chain STAPLE STRAND


Mass: 12919.359 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#89: DNA chain STAPLE STRAND


Mass: 15086.689 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#90: DNA chain STAPLE STRAND


Mass: 13323.549 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#91: DNA chain STAPLE STRAND


Mass: 11616.487 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#92: DNA chain STAPLE STRAND


Mass: 12848.345 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#93: DNA chain STAPLE STRAND


Mass: 14962.665 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#94: DNA chain STAPLE STRAND


Mass: 13046.363 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#95: DNA chain STAPLE STRAND


Mass: 15152.781 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#96: DNA chain STAPLE STRAND


Mass: 15714.158 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#97: DNA chain STAPLE STRAND


Mass: 10746.933 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#98: DNA chain STAPLE STRAND


Mass: 11358.335 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#99: DNA chain STAPLE STRAND


Mass: 12818.268 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#100: DNA chain STAPLE STRAND


Mass: 13394.583 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#101: DNA chain STAPLE STRAND


Mass: 12244.930 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#102: DNA chain STAPLE STRAND


Mass: 19435.473 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#103: DNA chain STAPLE STRAND


Mass: 10894.048 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#104: DNA chain STAPLE STRAND


Mass: 12945.377 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#105: DNA chain STAPLE STRAND


Mass: 12977.375 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#106: DNA chain STAPLE STRAND


Mass: 15156.748 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#107: DNA chain STAPLE STRAND


Mass: 10771.999 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#108: DNA chain STAPLE STRAND


Mass: 10792.953 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#109: DNA chain STAPLE STRAND


Mass: 12337.994 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#110: DNA chain STAPLE STRAND


Mass: 13437.621 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#111: DNA chain STAPLE STRAND


Mass: 15216.830 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#112: DNA chain STAPLE STRAND


Mass: 14486.345 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#113: DNA chain STAPLE STRAND


Mass: 12222.863 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#114: DNA chain STAPLE STRAND


Mass: 15286.876 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#115: DNA chain STAPLE STRAND


Mass: 13147.489 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#116: DNA chain STAPLE STRAND


Mass: 15293.793 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#117: DNA chain STAPLE STRAND


Mass: 11633.475 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#118: DNA chain STAPLE STRAND


Mass: 13458.683 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#119: DNA chain STAPLE STRAND


Mass: 10637.868 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#120: DNA chain STAPLE STRAND


Mass: 10735.944 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#121: DNA chain STAPLE STRAND


Mass: 15025.671 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#122: DNA chain STAPLE STRAND


Mass: 15200.831 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#123: DNA chain STAPLE STRAND


Mass: 12999.354 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#124: DNA chain STAPLE STRAND


Mass: 11017.115 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#125: DNA chain STAPLE STRAND


Mass: 10787.944 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#126: DNA chain STAPLE STRAND


Mass: 10704.934 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#127: DNA chain STAPLE STRAND


Mass: 15168.779 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#128: DNA chain STAPLE STRAND


Mass: 12727.230 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#129: DNA chain STAPLE STRAND


Mass: 10943.085 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#130: DNA chain STAPLE STRAND


Mass: 11573.445 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#131: DNA chain STAPLE STRAND


Mass: 15766.174 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#132: DNA chain STAPLE STRAND


Mass: 15378.920 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#133: DNA chain STAPLE STRAND


Mass: 13053.382 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#134: DNA chain STAPLE STRAND


Mass: 12160.843 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#135: DNA chain STAPLE STRAND


Mass: 11498.463 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#136: DNA chain STAPLE STRAND


Mass: 14477.383 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#137: DNA chain STAPLE STRAND


Mass: 11633.474 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#138: DNA chain STAPLE STRAND


Mass: 12412.030 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#139: DNA chain STAPLE STRAND


Mass: 12901.333 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#140: DNA chain STAPLE STRAND


Mass: 10755.946 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#141: DNA chain STAPLE STRAND


Mass: 12384.952 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#142: DNA chain STAPLE STRAND


Mass: 10775.897 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#143: DNA chain STAPLE STRAND


Mass: 15249.852 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#144: DNA chain STAPLE STRAND


Mass: 13123.448 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#145: DNA chain STAPLE STRAND


Mass: 13315.575 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#146: DNA chain STAPLE STRAND


Mass: 11609.449 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#147: DNA chain STAPLE STRAND


Mass: 12925.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#148: DNA chain STAPLE STRAND


Mass: 13036.414 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#149: DNA chain STAPLE STRAND


Mass: 15141.792 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#150: DNA chain STAPLE STRAND


Mass: 15074.656 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#151: DNA chain STAPLE STRAND


Mass: 13033.397 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#152: DNA chain STAPLE STRAND


Mass: 15172.733 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#153: DNA chain STAPLE STRAND


Mass: 12867.358 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#154: DNA chain STAPLE STRAND


Mass: 14492.394 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#155: DNA chain STAPLE STRAND


Mass: 13477.699 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#156: DNA chain STAPLE STRAND


Mass: 12443.939 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#157: DNA chain STAPLE STRAND


Mass: 11039.096 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#158: DNA chain STAPLE STRAND


Mass: 12867.255 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#159: DNA chain STAPLE STRAND


Mass: 17249.145 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#160: DNA chain STAPLE STRAND


Mass: 10742.911 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#161: DNA chain STAPLE STRAND


Mass: 10950.034 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#162: DNA chain STAPLE STRAND


Mass: 12471.031 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#163: DNA chain STAPLE STRAND


Mass: 13558.682 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#164: DNA chain STAPLE STRAND


Mass: 12835.200 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#165: DNA chain STAPLE STRAND


Mass: 10689.868 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#166: DNA chain STAPLE STRAND


Mass: 15145.691 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#167: DNA chain STAPLE STRAND


Mass: 13420.604 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#168: DNA chain STAPLE STRAND


Mass: 15189.721 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#169: DNA chain STAPLE STRAND


Mass: 13569.745 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#170: DNA chain STAPLE STRAND


Mass: 12971.325 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#171: DNA chain STAPLE STRAND


Mass: 11639.472 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#172: DNA chain STAPLE STRAND


Mass: 13360.555 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#173: DNA chain STAPLE STRAND


Mass: 10648.855 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#174: DNA chain STAPLE STRAND


Mass: 10935.005 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#175: DNA chain STAPLE STRAND


Mass: 13019.374 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#176: DNA chain STAPLE STRAND


Mass: 15125.671 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#177: DNA chain STAPLE STRAND


Mass: 12376.926 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#178: DNA chain STAPLE STRAND


Mass: 12982.369 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#179: DNA chain STAPLE STRAND


Mass: 15133.749 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#180: DNA chain STAPLE STRAND


Mass: 15163.772 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#181: DNA chain STAPLE STRAND


Mass: 13037.366 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#182: DNA chain STAPLE STRAND


Mass: 12839.275 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#183: DNA chain STAPLE STRAND


Mass: 11634.462 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#184: DNA chain STAPLE STRAND


Mass: 12196.861 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#185: DNA chain STAPLE STRAND


Mass: 11079.117 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#186: DNA chain STAPLE STRAND


Mass: 15240.714 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#187: DNA chain STAPLE STRAND


Mass: 13723.789 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#188: DNA chain STAPLE STRAND


Mass: 12894.346 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#189: DNA chain STAPLE STRAND


Mass: 10677.875 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#190: DNA chain STAPLE STRAND


Mass: 14474.350 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#191: DNA chain STAPLE STRAND


Mass: 11412.367 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#192: DNA chain STAPLE STRAND


Mass: 11655.471 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#193: DNA chain STAPLE STRAND


Mass: 14953.543 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#194: DNA chain STAPLE STRAND


Mass: 13035.374 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#195: DNA chain STAPLE STRAND


Mass: 12465.001 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#196: DNA chain STAPLE STRAND


Mass: 12851.255 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#197: DNA chain STAPLE STRAND


Mass: 10793.979 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#198: DNA chain STAPLE STRAND


Mass: 13450.605 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#199: DNA chain STAPLE STRAND


Mass: 11667.448 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#200: DNA chain STAPLE STRAND


Mass: 13075.383 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#201: DNA chain STAPLE STRAND


Mass: 14957.566 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#202: DNA chain STAPLE STRAND


Mass: 10721.867 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#203: DNA chain STAPLE STRAND


Mass: 15106.708 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#204: DNA chain STAPLE STRAND


Mass: 15372.852 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#205: DNA chain STAPLE STRAND


Mass: 14974.605 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#206: DNA chain STAPLE STRAND


Mass: 11394.286 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#207: DNA chain STAPLE STRAND


Mass: 12911.246 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#208: DNA chain STAPLE STRAND


Mass: 15253.731 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#209: DNA chain STAPLE STRAND


Mass: 13483.644 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#210: DNA chain STAPLE STRAND


Mass: 12275.871 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#211: DNA chain STAPLE STRAND


Mass: 12921.301 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#212: DNA chain STAPLE STRAND


Mass: 12871.328 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: 48 helix bundle DNA origami brick , multilayer DNA origami object
Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: synthetic construct (others)
Source (recombinant)Organism: synthetic construct (others)
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 50 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON III (4k x 4k)

-
Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 10 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 31931 / Symmetry type: POINT

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