CagY type 1 repeat / CagY type 1 repeat / DC-EC / DC-EC Repeat / Type IV secretion system CagX conjugation protein / Conjugal transfer, TrbG/VirB9/CagX / VirB9/CagX/TrbG, C-terminal / VirB9/CagX/TrbG, C-terminal domain superfamily / Conjugal transfer protein / Type IV secretion system, VirB10/TrbI ...CagY type 1 repeat / CagY type 1 repeat / DC-EC / DC-EC Repeat / Type IV secretion system CagX conjugation protein / Conjugal transfer, TrbG/VirB9/CagX / VirB9/CagX/TrbG, C-terminal / VirB9/CagX/TrbG, C-terminal domain superfamily / Conjugal transfer protein / Type IV secretion system, VirB10/TrbI / Bacterial conjugation TrbI-like protein / Type IV secretion system, VirB10 / TraB / TrbI 類似検索 - ドメイン・相同性
Cag pathogenicity island protein (Cag7) / Cag pathogenicity island protein (Cag8) 類似検索 - 構成要素
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI118932
米国
National Institutes of Health/National Cancer Institute (NIH/NCI)
CA116087
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI039657
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM103310
米国
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)
2T32DK007673
米国
National Institutes of Health/Office of the Director
S10OD020011
米国
Other government
1I01BX004447
米国
引用
ジャーナル: Elife / 年: 2020 タイトル: Cryo-EM reveals species-specific components within the Cag type IV secretion system core complex. 著者: Michael J Sheedlo / Jeong Min Chung / Neha Sawhney / Clarissa L Durie / Timothy L Cover / Melanie D Ohi / D Borden Lacy / 要旨: The pathogenesis of -associated gastric cancer is dependent on delivery of CagA into host cells through a type IV secretion system (T4SS). The Cag T4SS includes a large membrane-spanning core ...The pathogenesis of -associated gastric cancer is dependent on delivery of CagA into host cells through a type IV secretion system (T4SS). The Cag T4SS includes a large membrane-spanning core complex containing five proteins, organized into an outer membrane cap (OMC), a periplasmic ring (PR) and a stalk. Here, we report cryo-EM reconstructions of a core complex lacking Cag3 and an improved map of the wild-type complex. We define the structures of two unique species-specific components (Cag3 and CagM) and show that Cag3 is structurally similar to CagT. Unexpectedly, components of the OMC are organized in a 1:1:2:2:5 molar ratio (CagY:CagX:CagT:CagM:Cag3). CagX and CagY are components of both the OMC and the PR and bridge the symmetry mismatch between these regions. These results reveal that assembly of the T4SS core complex is dependent on incorporation of interwoven species-specific components.
AX: Cag pathogenicity island protein (Cag8) BX: Cag pathogenicity island protein (Cag8) CX: Cag pathogenicity island protein (Cag8) DX: Cag pathogenicity island protein (Cag8) EX: Cag pathogenicity island protein (Cag8) FX: Cag pathogenicity island protein (Cag8) GX: Cag pathogenicity island protein (Cag8) HX: Cag pathogenicity island protein (Cag8) IX: Cag pathogenicity island protein (Cag8) JX: Cag pathogenicity island protein (Cag8) KX: Cag pathogenicity island protein (Cag8) LX: Cag pathogenicity island protein (Cag8) MX: Cag pathogenicity island protein (Cag8) NX: Cag pathogenicity island protein (Cag8) OX: Cag pathogenicity island protein (Cag8) PX: Cag pathogenicity island protein (Cag8) QX: Cag pathogenicity island protein (Cag8) AY: Cag pathogenicity island protein (Cag7) BY: Cag pathogenicity island protein (Cag7) CY: Cag pathogenicity island protein (Cag7) DY: Cag pathogenicity island protein (Cag7) EY: Cag pathogenicity island protein (Cag7) FY: Cag pathogenicity island protein (Cag7) GY: Cag pathogenicity island protein (Cag7) HY: Cag pathogenicity island protein (Cag7) IY: Cag pathogenicity island protein (Cag7) JY: Cag pathogenicity island protein (Cag7) KY: Cag pathogenicity island protein (Cag7) LY: Cag pathogenicity island protein (Cag7) MY: Cag pathogenicity island protein (Cag7) NY: Cag pathogenicity island protein (Cag7) OY: Cag pathogenicity island protein (Cag7) PY: Cag pathogenicity island protein (Cag7) QY: Cag pathogenicity island protein (Cag7)