Cag pathogenicity island protein Cag12 / Cag pathogenicity island protein Cag12 / CagY type 1 repeat / CagY type 1 repeat / DC-EC / DC-EC Repeat / Type IV secretion system CagX conjugation protein / Conjugal transfer, TrbG/VirB9/CagX / VirB9/CagX/TrbG, C-terminal / VirB9/CagX/TrbG, C-terminal domain superfamily ...Cag pathogenicity island protein Cag12 / Cag pathogenicity island protein Cag12 / CagY type 1 repeat / CagY type 1 repeat / DC-EC / DC-EC Repeat / Type IV secretion system CagX conjugation protein / Conjugal transfer, TrbG/VirB9/CagX / VirB9/CagX/TrbG, C-terminal / VirB9/CagX/TrbG, C-terminal domain superfamily / Conjugal transfer protein / Type IV secretion system, VirB10/TrbI / Bacterial conjugation TrbI-like protein / Type IV secretion system, VirB10 / TraB / TrbI / Prokaryotic membrane lipoprotein lipid attachment site profile. 類似検索 - ドメイン・相同性
Type IV secretion system apparatus protein CagX / Cag pathogenicity island protein (Cag7) / Cag pathogenicity island protein 類似検索 - 構成要素
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI118932
米国
National Institutes of Health/National Cancer Institute (NIH/NCI)
CA116087
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI039657
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM103310
米国
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)
2T32DK007673
米国
National Institutes of Health/Office of the Director
S10OD020011
米国
Other government
1I01BX004447
米国
引用
ジャーナル: Elife / 年: 2020 タイトル: Cryo-EM reveals species-specific components within the Cag type IV secretion system core complex. 著者: Michael J Sheedlo / Jeong Min Chung / Neha Sawhney / Clarissa L Durie / Timothy L Cover / Melanie D Ohi / D Borden Lacy / 要旨: The pathogenesis of -associated gastric cancer is dependent on delivery of CagA into host cells through a type IV secretion system (T4SS). The Cag T4SS includes a large membrane-spanning core ...The pathogenesis of -associated gastric cancer is dependent on delivery of CagA into host cells through a type IV secretion system (T4SS). The Cag T4SS includes a large membrane-spanning core complex containing five proteins, organized into an outer membrane cap (OMC), a periplasmic ring (PR) and a stalk. Here, we report cryo-EM reconstructions of a core complex lacking Cag3 and an improved map of the wild-type complex. We define the structures of two unique species-specific components (Cag3 and CagM) and show that Cag3 is structurally similar to CagT. Unexpectedly, components of the OMC are organized in a 1:1:2:2:5 molar ratio (CagY:CagX:CagT:CagM:Cag3). CagX and CagY are components of both the OMC and the PR and bridge the symmetry mismatch between these regions. These results reveal that assembly of the T4SS core complex is dependent on incorporation of interwoven species-specific components.
AT: Cag pathogenicity island protein AX: Type IV secretion system apparatus protein CagX AY: Cag pathogenicity island protein (Cag7) BT: Cag pathogenicity island protein BX: Type IV secretion system apparatus protein CagX BY: Cag pathogenicity island protein (Cag7) CT: Cag pathogenicity island protein CX: Type IV secretion system apparatus protein CagX CY: Cag pathogenicity island protein (Cag7) DT: Cag pathogenicity island protein DX: Type IV secretion system apparatus protein CagX DY: Cag pathogenicity island protein (Cag7) ET: Cag pathogenicity island protein EX: Type IV secretion system apparatus protein CagX EY: Cag pathogenicity island protein (Cag7) FT: Cag pathogenicity island protein FX: Type IV secretion system apparatus protein CagX FY: Cag pathogenicity island protein (Cag7) GT: Cag pathogenicity island protein GX: Type IV secretion system apparatus protein CagX GY: Cag pathogenicity island protein (Cag7) HT: Cag pathogenicity island protein HX: Type IV secretion system apparatus protein CagX HY: Cag pathogenicity island protein (Cag7) IT: Cag pathogenicity island protein IX: Type IV secretion system apparatus protein CagX IY: Cag pathogenicity island protein (Cag7) JT: Cag pathogenicity island protein JX: Type IV secretion system apparatus protein CagX JY: Cag pathogenicity island protein (Cag7) KT: Cag pathogenicity island protein KX: Type IV secretion system apparatus protein CagX KY: Cag pathogenicity island protein (Cag7) LT: Cag pathogenicity island protein LX: Type IV secretion system apparatus protein CagX LY: Cag pathogenicity island protein (Cag7) MT: Cag pathogenicity island protein MX: Type IV secretion system apparatus protein CagX MY: Cag pathogenicity island protein (Cag7) NT: Cag pathogenicity island protein NX: Type IV secretion system apparatus protein CagX NY: Cag pathogenicity island protein (Cag7)