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Open data
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Basic information
Entry | Database: PDB / ID: 6wfj | ||||||
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Title | Crystal structures of human E-NPP 1: apo | ||||||
![]() | Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 | ||||||
![]() | HYDROLASE / human E-NPP 1 / drug discovery / inhibitors | ||||||
Function / homology | ![]() GTP diphosphatase activity / cyclic-GMP-AMP hydrolase activity / negative regulation of hh target transcription factor activity / Vitamin B2 (riboflavin) metabolism / UTP diphosphatase activity / inorganic diphosphate transport / phosphodiesterase I / 3'-phosphoadenosine 5'-phosphosulfate binding / dinucleotide phosphatase activity / Vitamin B5 (pantothenate) metabolism ...GTP diphosphatase activity / cyclic-GMP-AMP hydrolase activity / negative regulation of hh target transcription factor activity / Vitamin B2 (riboflavin) metabolism / UTP diphosphatase activity / inorganic diphosphate transport / phosphodiesterase I / 3'-phosphoadenosine 5'-phosphosulfate binding / dinucleotide phosphatase activity / Vitamin B5 (pantothenate) metabolism / nucleotide diphosphatase / nucleoside triphosphate catabolic process / 3'-phosphoadenosine 5'-phosphosulfate metabolic process / negative regulation of protein autophosphorylation / nucleoside triphosphate diphosphatase activity / intracellular phosphate ion homeostasis / sequestering of triglyceride / nucleic acid metabolic process / ATP diphosphatase activity / negative regulation of glycogen biosynthetic process / phosphate ion homeostasis / negative regulation of bone mineralization / melanocyte differentiation / phosphodiesterase I activity / scavenger receptor activity / negative regulation of glucose import / regulation of bone mineralization / phosphate-containing compound metabolic process / exonuclease activity / polysaccharide binding / response to ATP / negative regulation of fat cell differentiation / bone mineralization / phosphatase activity / : / 3',5'-cyclic-AMP phosphodiesterase activity / ATP metabolic process / negative regulation of insulin receptor signaling pathway / generation of precursor metabolites and energy / insulin receptor binding / negative regulation of cell growth / cellular response to insulin stimulus / gene expression / basolateral plasma membrane / nucleic acid binding / immune response / lysosomal membrane / calcium ion binding / cell surface / protein homodimerization activity / extracellular space / zinc ion binding / ATP binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Peat, T.S. / Dennis, M. / Newman, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structures of human ENPP1 in apo and bound forms. Authors: Dennis, M.L. / Newman, J. / Dolezal, O. / Hattarki, M. / Surjadi, R.N. / Nuttall, S.D. / Pham, T. / Nebl, T. / Camerino, M. / Khoo, P.S. / Monahan, B.J. / Peat, T.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 362.8 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.3 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 57.3 KB | Display | |
Data in CIF | ![]() | 79.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6wetSC ![]() 6weuC ![]() 6wevC ![]() 6wewC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AcABcB
#1: Protein | Mass: 105137.375 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() References: UniProt: P22413, phosphodiesterase I, nucleotide diphosphatase |
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-Sugars , 5 types, 9 molecules ![](data/chem/img/NAG.gif)
#2: Polysaccharide | beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4) ...beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||||
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#3: Polysaccharide | alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||||
#4: Polysaccharide | Source method: isolated from a genetically manipulated source #5: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #8: Sugar | ChemComp-NAG / |
-Non-polymers , 6 types, 226 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/AMP.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/AMP.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
#6: Chemical | ChemComp-ZN / #7: Chemical | #9: Chemical | #10: Chemical | ChemComp-PEG / | #11: Chemical | #12: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.3 Å3/Da / Density % sol: 62.67 % |
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Crystal grow | Temperature: 281 K / Method: vapor diffusion, sitting drop Details: 7.5 mg/mL protein against 19-22% PEG4000, 240-270 mM trilithium/triammonium/tripotassium citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Sep 28, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.953731 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→47.487 Å / Num. obs: 96772 / % possible obs: 99.9 % / Redundancy: 26.1 % / CC1/2: 0.999 / Rpim(I) all: 0.041 / Net I/σ(I): 15.1 |
Reflection shell | Resolution: 2.5→2.54 Å / Mean I/σ(I) obs: 1.3 / Num. unique obs: 4657 / CC1/2: 0.65 / Rpim(I) all: 0.567 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 6WET Resolution: 2.5→47.44 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.94 / SU B: 8.407 / SU ML: 0.179 / Cross valid method: FREE R-VALUE / ESU R: 0.298 / ESU R Free: 0.215 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 60.345 Å2
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Refinement step | Cycle: LAST / Resolution: 2.5→47.44 Å
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Refine LS restraints |
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LS refinement shell |
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