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- PDB-6vbm: Crystal structure of a S310A mutant of PBP2 from Neisseria gonorrhoeae -
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Open data
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Basic information
Entry | Database: PDB / ID: 6vbm | ||||||
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Title | Crystal structure of a S310A mutant of PBP2 from Neisseria gonorrhoeae | ||||||
![]() | Probable peptidoglycan D,D-transpeptidase PenA | ||||||
![]() | HYDROLASE / PENICILLIN-BINDING PROTEIN / TRANSPEPTIDASE DOMAIN / ANTIBIOTIC RESISTANCE | ||||||
Function / homology | ![]() peptidoglycan glycosyltransferase activity / serine-type D-Ala-D-Ala carboxypeptidase / FtsZ-dependent cytokinesis / serine-type D-Ala-D-Ala carboxypeptidase activity / division septum assembly / penicillin binding / peptidoglycan biosynthetic process / cell wall organization / regulation of cell shape / response to antibiotic ...peptidoglycan glycosyltransferase activity / serine-type D-Ala-D-Ala carboxypeptidase / FtsZ-dependent cytokinesis / serine-type D-Ala-D-Ala carboxypeptidase activity / division septum assembly / penicillin binding / peptidoglycan biosynthetic process / cell wall organization / regulation of cell shape / response to antibiotic / proteolysis / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Singh, A. / Davies, C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Mutations in Neisseria gonorrhoeae penicillin-binding protein 2 associated with extended-spectrum cephalosporin resistance create an energetic barrier against acylation via restriction of protein dynamics Authors: Singh, A. / Turner, J. / Tomberg, J. / Fedarovich, A. / Unemo, M. / Nicholas, R.A. / Davies, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 137 KB | Display | ![]() |
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PDB format | ![]() | 105.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 264.8 KB | Display | ![]() |
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Full document | ![]() | 264.7 KB | Display | |
Data in XML | ![]() | 1.1 KB | Display | |
Data in CIF | ![]() | 7.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6vbcC ![]() 6vbdC ![]() 6vblC ![]() 6p53S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35310.262 Da / Num. of mol.: 2 / Mutation: S310A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P08149, serine-type D-Ala-D-Ala carboxypeptidase #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.72 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 9.3 / Details: 0.1 M CHES, 40% PEG 600 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 22, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.71→39.1 Å / Num. obs: 63070 / % possible obs: 98.6 % / Redundancy: 5.1 % / CC1/2: 0.99 / Rmerge(I) obs: 0.069 / Rpim(I) all: 0.034 / Net I/σ(I): 45.9 |
Reflection shell | Resolution: 1.71→1.75 Å / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 3 / Num. unique obs: 2904 / CC1/2: 0.904 / Rpim(I) all: 0.206 / % possible all: 92.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6P53 Resolution: 1.71→39.1 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.955 / SU B: 3.139 / SU ML: 0.098 / Cross valid method: THROUGHOUT / ESU R: 0.121 / ESU R Free: 0.113
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.074 Å2
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Refinement step | Cycle: 1 / Resolution: 1.71→39.1 Å
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Refine LS restraints |
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