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- PDB-6sj8: Methyltransferase MtgA from Desulfitobacterium hafniense in compl... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6sj8 | ||||||
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Title | Methyltransferase MtgA from Desulfitobacterium hafniense in complex with tetrahydrofolate | ||||||
![]() | Tetrahydromethanopterin S-methyltransferase | ||||||
![]() | TRANSFERASE / Anaerobic Bacteria / Glycine Betaine Metabolism / Methyl Transfer / Cobalamin / Tetrahydrofolate | ||||||
Function / homology | Tetrahydromethanopterin S-methyltransferase, subunit H/Methyltransferase Mtx, subunit H / Tetrahydromethanopterin S-methyltransferase MtrH subunit / tetrahydromethanopterin S-methyltransferase activity / tetrahydromethanopterin S-methyltransferase / Dihydropteroate synthase-like / one-carbon metabolic process / methylation / Chem-44V / Tetrahydromethanopterin S-methyltransferase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Badmann, T. / Groll, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structures in Tetrahydrofolate Methylation in Desulfitobacterial Glycine Betaine Metabolism at Atomic Resolution. Authors: Badmann, T. / Groll, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 270.5 KB | Display | ![]() |
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PDB format | ![]() | 218.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 999.1 KB | Display | ![]() |
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Full document | ![]() | 1000.8 KB | Display | |
Data in XML | ![]() | 29 KB | Display | |
Data in CIF | ![]() | 44.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6sjkC ![]() 6sjnC ![]() 6sjoC ![]() 6sjpC ![]() 6sjrC ![]() 6sjsC ![]() 6sk4C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 33508.270 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: Dhaf_4327 / Plasmid: pET-28b(+) / Production host: ![]() ![]() References: UniProt: B8FUR2, tetrahydromethanopterin S-methyltransferase |
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-Non-polymers , 5 types, 588 molecules ![](data/chem/img/44V.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | #4: Chemical | ChemComp-GOL / #5: Chemical | ChemComp-SO4 / | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.25 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 100 mM HEPES, 27% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 23, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.35→30 Å / Num. obs: 120092 / % possible obs: 99.1 % / Redundancy: 4.1 % / CC1/2: 0.99 / Rmerge(I) obs: 0.048 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 1.35→1.45 Å / Rmerge(I) obs: 0.645 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 23073 / CC1/2: 0.74 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 73.66 Å2 / Biso mean: 19.422 Å2 / Biso min: 9.95 Å2
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Refinement step | Cycle: final / Resolution: 1.35→15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.35→1.385 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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