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- PDB-6sbv: X-ray Structure of Human LDH-A with an Allosteric Inhibitor (Comp... -

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Basic information

Entry
Database: PDB / ID: 6sbv
TitleX-ray Structure of Human LDH-A with an Allosteric Inhibitor (Compound 7)
ComponentsL-lactate dehydrogenase A chain
KeywordsOXIDOREDUCTASE / LDHA / OXIDOREDUCTASE INHIBITOR / ALLOSTERIC INHIBITOR
Function / homology
Function and homology information


sperm fibrous sheath / pyruvate catabolic process / L-lactate dehydrogenase / oxidoreductase complex / L-lactate dehydrogenase (NAD+) activity / Pyruvate metabolism / pyruvate metabolic process / lactate metabolic process / glucose catabolic process to lactate via pyruvate / Regulation of pyruvate metabolism ...sperm fibrous sheath / pyruvate catabolic process / L-lactate dehydrogenase / oxidoreductase complex / L-lactate dehydrogenase (NAD+) activity / Pyruvate metabolism / pyruvate metabolic process / lactate metabolic process / glucose catabolic process to lactate via pyruvate / Regulation of pyruvate metabolism / substantia nigra development / glycolytic process / cadherin binding / mitochondrion / extracellular exosome / identical protein binding / nucleus / membrane / cytosol
Similarity search - Function
L-lactate dehydrogenase / L-lactate dehydrogenase active site. / L-lactate dehydrogenase, active site / L-2-Hydroxyisocaproate Dehydrogenase; Chain A, domain 2 / Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal / L-lactate/malate dehydrogenase / Lactate/malate dehydrogenase, N-terminal / Lactate/malate dehydrogenase, C-terminal / lactate/malate dehydrogenase, NAD binding domain / lactate/malate dehydrogenase, alpha/beta C-terminal domain ...L-lactate dehydrogenase / L-lactate dehydrogenase active site. / L-lactate dehydrogenase, active site / L-2-Hydroxyisocaproate Dehydrogenase; Chain A, domain 2 / Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal / L-lactate/malate dehydrogenase / Lactate/malate dehydrogenase, N-terminal / Lactate/malate dehydrogenase, C-terminal / lactate/malate dehydrogenase, NAD binding domain / lactate/malate dehydrogenase, alpha/beta C-terminal domain / Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal / NAD(P)-binding Rossmann-like Domain / NAD(P)-binding domain superfamily / Alpha-Beta Complex / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Chem-L5K / L-lactate dehydrogenase A chain
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å
AuthorsFriberg, A. / Puetter, V. / Nguyen, D. / Rehwinkel, H.
CitationJournal: Acs Omega / Year: 2020
Title: Structural Evidence for Isoform-Selective Allosteric Inhibition of Lactate Dehydrogenase A.
Authors: Friberg, A. / Rehwinkel, H. / Nguyen, D. / Putter, V. / Quanz, M. / Weiske, J. / Eberspacher, U. / Heisler, I. / Langer, G.
History
DepositionJul 22, 2019Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 10, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 1, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.2Jan 24, 2024Group: Advisory / Data collection ...Advisory / Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_unobs_or_zero_occ_atoms / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: L-lactate dehydrogenase A chain
B: L-lactate dehydrogenase A chain
C: L-lactate dehydrogenase A chain
D: L-lactate dehydrogenase A chain
E: L-lactate dehydrogenase A chain
F: L-lactate dehydrogenase A chain
G: L-lactate dehydrogenase A chain
H: L-lactate dehydrogenase A chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)297,02416
Polymers293,2848
Non-polymers3,7408
Water7,044391
1
A: L-lactate dehydrogenase A chain
B: L-lactate dehydrogenase A chain
C: L-lactate dehydrogenase A chain
D: L-lactate dehydrogenase A chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)148,5128
Polymers146,6424
Non-polymers1,8704
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area19790 Å2
ΔGint-118 kcal/mol
Surface area48360 Å2
MethodPISA
2
E: L-lactate dehydrogenase A chain
F: L-lactate dehydrogenase A chain
G: L-lactate dehydrogenase A chain
H: L-lactate dehydrogenase A chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)148,5128
Polymers146,6424
Non-polymers1,8704
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area19700 Å2
ΔGint-120 kcal/mol
Surface area48530 Å2
MethodPISA
Unit cell
Length a, b, c (Å)80.369, 146.287, 308.362
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11A
21B
12A
22C
13A
23D
14A
24E
15A
25F
16A
26G
17A
27H
18B
28C
19B
29D
110B
210E
111B
211F
112B
212G
113B
213H
114C
214D
115C
215E
116C
216F
117C
217G
118C
218H
119D
219E
120D
220F
121D
221G
122D
222H
123E
223F
124E
224G
125E
225H
126F
226G
127F
227H
128G
228H

NCS domain segments:

Component-ID: _ / Beg auth comp-ID: ALA / Beg label comp-ID: ALA / End auth comp-ID: PHE / End label comp-ID: PHE / Refine code: _ / Auth seq-ID: 2 - 332 / Label seq-ID: 2 - 332

Dom-IDEns-IDAuth asym-IDLabel asym-ID
11AA
21BB
12AA
22CC
13AA
23DD
14AA
24EE
15AA
25FF
16AA
26GG
17AA
27HH
18BB
28CC
19BB
29DD
110BB
210EE
111BB
211FF
112BB
212GG
113BB
213HH
114CC
214DD
115CC
215EE
116CC
216FF
117CC
217GG
118CC
218HH
119DD
219EE
120DD
220FF
121DD
221GG
122DD
222HH
123EE
223FF
124EE
224GG
125EE
225HH
126FF
226GG
127FF
227HH
128GG
228HH

NCS ensembles :
ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28

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Components

#1: Protein
L-lactate dehydrogenase A chain / LDH-A / Cell proliferation-inducing gene 19 protein / LDH muscle subunit / LDH-M / Renal carcinoma ...LDH-A / Cell proliferation-inducing gene 19 protein / LDH muscle subunit / LDH-M / Renal carcinoma antigen NY-REN-59


Mass: 36660.527 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: LDHA, PIG19 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P00338, L-lactate dehydrogenase
#2: Chemical
ChemComp-L5K / ~{N}-[3-[(7-nitrodibenzofuran-2-yl)sulfonylamino]phenyl]-1-oxidanyl-cyclopropane-1-carboxamide


Mass: 467.451 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C22H17N3O7S / Feature type: SUBJECT OF INVESTIGATION
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 391 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.09 Å3/Da / Density % sol: 60.2 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop
Details: 0.1 M HEPES pH 7.5-7.7, 4-8% PEG 8000, 20% ethylene glycol, and 10% acetonitrile
PH range: 7.5-7.7

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.918409 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 22, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.918409 Å / Relative weight: 1
ReflectionResolution: 2.6→50 Å / Num. obs: 112371 / % possible obs: 99.5 % / Redundancy: 5.4 % / CC1/2: 0.991 / Rrim(I) all: 0.225 / Net I/σ(I): 6.18
Reflection shellResolution: 2.6→2.76 Å / Redundancy: 5.4 % / Mean I/σ(I) obs: 1.63 / Num. unique obs: 17771 / CC1/2: 0.797 / Rrim(I) all: 0.861 / % possible all: 98.7

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Processing

Software
NameVersionClassification
REFMAC5.8.0238refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4l4r

4l4r


Resolution: 2.6→48.93 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.927 / SU B: 14.316 / SU ML: 0.268 / Cross valid method: THROUGHOUT / ESU R: 0.515 / ESU R Free: 0.277 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.24233 2101 1.9 %RANDOM
Rwork0.2191 ---
obs0.21954 110270 99.54 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å
Displacement parametersBiso mean: 43.504 Å2
Baniso -1Baniso -2Baniso -3
1-4.22 Å20 Å20 Å2
2---0.08 Å20 Å2
3----4.14 Å2
Refinement stepCycle: LAST / Resolution: 2.6→48.93 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms20374 0 264 391 21029
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0050.01321017
X-RAY DIFFRACTIONr_bond_other_d0.0010.01720391
X-RAY DIFFRACTIONr_angle_refined_deg1.2871.64528478
X-RAY DIFFRACTIONr_angle_other_deg1.1611.58447201
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.63552616
X-RAY DIFFRACTIONr_dihedral_angle_2_deg38.60923.499886
X-RAY DIFFRACTIONr_dihedral_angle_3_deg15.71153876
X-RAY DIFFRACTIONr_dihedral_angle_4_deg21.3661588
X-RAY DIFFRACTIONr_chiral_restr0.0520.22735
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.0222865
X-RAY DIFFRACTIONr_gen_planes_other0.0010.023919
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it2.354.46510506
X-RAY DIFFRACTIONr_mcbond_other2.354.46510505
X-RAY DIFFRACTIONr_mcangle_it3.7676.6913105
X-RAY DIFFRACTIONr_mcangle_other3.7676.6913106
X-RAY DIFFRACTIONr_scbond_it2.7294.86910511
X-RAY DIFFRACTIONr_scbond_other2.7284.86910512
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other4.5457.14415348
X-RAY DIFFRACTIONr_long_range_B_refined6.34751.33722807
X-RAY DIFFRACTIONr_long_range_B_other6.34451.34122795
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05

Ens-IDDom-IDAuth asym-IDNumberRms dev position (Å)
11A101040.05
12B101040.05
21A101220.04
22C101220.04
31A101170.05
32D101170.05
41A101610.04
42E101610.04
51A101380.05
52F101380.05
61A101560.04
62G101560.04
71A101230.05
72H101230.05
81B100830.05
82C100830.05
91B101140.05
92D101140.05
101B100980.05
102E100980.05
111B101520.04
112F101520.04
121B101160.05
122G101160.05
131B101340.04
132H101340.04
141C101160.05
142D101160.05
151C101590.04
152E101590.04
161C101050.05
162F101050.05
171C101560.04
172G101560.04
181C101290.04
182H101290.04
191D101490.04
192E101490.04
201D101440.04
202F101440.04
211D101250.04
212G101250.04
221D101680.04
222H101680.04
231E101510.04
232F101510.04
241E101520.05
242G101520.05
251E101630.04
252H101630.04
261F101470.04
262G101470.04
271F101450.04
272H101450.04
281G101380.04
282H101380.04
LS refinement shellResolution: 2.598→2.666 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.339 149 -
Rwork0.367 7826 -
obs--97.43 %

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