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Yorodumi- PDB-6rnv: The crystal structure of Thermosynechococcus elongatus protochlor... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6rnv | |||||||||||||||||||||
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Title | The crystal structure of Thermosynechococcus elongatus protochlorophyllide oxidoreductase (POR) | |||||||||||||||||||||
Components | Thermosynechococcus elongatus protochlorophyllide oxidoreductase (POR) | |||||||||||||||||||||
Keywords | PHOTOSYNTHESIS / Light dependent | |||||||||||||||||||||
Function / homology | protochlorophyllide reductase / protochlorophyllide reductase activity / Light-dependent protochlorophyllide reductase / chlorophyll biosynthetic process / short chain dehydrogenase / photosynthesis / Short-chain dehydrogenase/reductase SDR / NAD(P)-binding domain superfamily / protochlorophyllide reductase Function and homology information | |||||||||||||||||||||
Biological species | Thermosynechococcus elongatus (bacteria) | |||||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.27 Å | |||||||||||||||||||||
Authors | Levy, C.W. | |||||||||||||||||||||
Funding support | United Kingdom, China, 6items
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Citation | Journal: Nature / Year: 2019 Title: Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis. Authors: Zhang, S. / Heyes, D.J. / Feng, L. / Sun, W. / Johannissen, L.O. / Liu, H. / Levy, C.W. / Li, X. / Yang, J. / Yu, X. / Lin, M. / Hardman, S.J.O. / Hoeven, R. / Sakuma, M. / Hay, S. / Leys, D. ...Authors: Zhang, S. / Heyes, D.J. / Feng, L. / Sun, W. / Johannissen, L.O. / Liu, H. / Levy, C.W. / Li, X. / Yang, J. / Yu, X. / Lin, M. / Hardman, S.J.O. / Hoeven, R. / Sakuma, M. / Hay, S. / Leys, D. / Rao, Z. / Zhou, A. / Cheng, Q. / Scrutton, N.S. | |||||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6rnv.cif.gz | 216.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6rnv.ent.gz | 150.5 KB | Display | PDB format |
PDBx/mmJSON format | 6rnv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rn/6rnv ftp://data.pdbj.org/pub/pdb/validation_reports/rn/6rnv | HTTPS FTP |
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-Related structure data
Related structure data | 6r46C 6r48C 6rnwC 3rd5S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 36973.625 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermosynechococcus elongatus (bacteria) Production host: Escherichia coli (E. coli) / References: UniProt: Q8DLC1*PLUS | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.29 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.01 M zinc chloride, 0.1 M sodium acetate pH 5.0, 20 % w/v PEG 6K Temp details: Cold room |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.9159 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 2, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9159 Å / Relative weight: 1 |
Reflection | Resolution: 1.27→53.08 Å / Num. obs: 91463 / % possible obs: 98.76 % / Redundancy: 17.5 % / Biso Wilson estimate: 14.04 Å2 / CC1/2: 0.993 / Rmerge(I) obs: 0.0928 / Rpim(I) all: 0.02207 / Rrim(I) all: 0.09546 / Net I/σ(I): 14.73 |
Reflection shell | Resolution: 1.27→1.315 Å / Redundancy: 12.1 % / Rmerge(I) obs: 0.8746 / Mean I/σ(I) obs: 2.36 / Num. unique obs: 9068 / CC1/2: 0.541 / Rpim(I) all: 0.2592 / Rrim(I) all: 0.9132 / % possible all: 96.46 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3RD5 Resolution: 1.27→53.08 Å / SU ML: 0.157 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 19.7851
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.19 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.27→53.08 Å
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Refine LS restraints |
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LS refinement shell |
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