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- PDB-6rlp: Cryo-EM reconstruction of TMV coat protein -

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Basic information

Entry
Database: PDB / ID: 6rlp
TitleCryo-EM reconstruction of TMV coat protein
Components
  • Capsid protein
  • RNA (5'-R(P*GP*AP*A)-3')
KeywordsVIRAL PROTEIN / cryo-EM / single particle reconstruction / Tobacco mosaic virus / helical reconstruction / ESRF / Titan Krios / K2 Summit
Function / homologyTobacco mosaic virus-like, coat protein / Tobacco mosaic virus-like, coat protein superfamily / Virus coat protein (TMV like) / helical viral capsid / structural molecule activity / RNA / Capsid protein
Function and homology information
Biological speciesTobacco mosaic virus
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 2.3 Å
AuthorsKandiah, E. / Effantin, G.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Acta Crystallogr D Struct Biol / Year: 2019
Title: CM01: a facility for cryo-electron microscopy at the European Synchrotron.
Authors: Eaazhisai Kandiah / Thierry Giraud / Alejandro de Maria Antolinos / Fabien Dobias / Gregory Effantin / David Flot / Michael Hons / Guy Schoehn / Jean Susini / Olof Svensson / Gordon A ...Authors: Eaazhisai Kandiah / Thierry Giraud / Alejandro de Maria Antolinos / Fabien Dobias / Gregory Effantin / David Flot / Michael Hons / Guy Schoehn / Jean Susini / Olof Svensson / Gordon A Leonard / Christoph Mueller-Dieckmann /
Abstract: Recent improvements in direct electron detectors, microscope technology and software provided the stimulus for a `quantum leap' in the application of cryo-electron microscopy in structural biology, ...Recent improvements in direct electron detectors, microscope technology and software provided the stimulus for a `quantum leap' in the application of cryo-electron microscopy in structural biology, and many national and international centres have since been created in order to exploit this. Here, a new facility for cryo-electron microscopy focused on single-particle reconstruction of biological macromolecules that has been commissioned at the European Synchrotron Radiation Facility (ESRF) is presented. The facility is operated by a consortium of institutes co-located on the European Photon and Neutron Campus and is managed in a similar fashion to a synchrotron X-ray beamline. It has been open to the ESRF structural biology user community since November 2017 and will remain open during the 2019 ESRF-EBS shutdown.
History
DepositionMay 2, 2019Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 23, 2022Provider: repository / Type: Initial release
Revision 1.1Jun 12, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / citation_author
Item: _citation.journal_abbrev / _citation.journal_id_CSD ..._citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Capsid protein
R: RNA (5'-R(P*GP*AP*A)-3')
B: Capsid protein
Z: RNA (5'-R(P*GP*AP*A)-3')
C: Capsid protein
a: RNA (5'-R(P*GP*AP*A)-3')
D: Capsid protein
b: RNA (5'-R(P*GP*AP*A)-3')
E: Capsid protein
c: RNA (5'-R(P*GP*AP*A)-3')
F: Capsid protein
d: RNA (5'-R(P*GP*AP*A)-3')
G: Capsid protein
e: RNA (5'-R(P*GP*AP*A)-3')
H: Capsid protein
f: RNA (5'-R(P*GP*AP*A)-3')
I: Capsid protein
g: RNA (5'-R(P*GP*AP*A)-3')
J: Capsid protein
h: RNA (5'-R(P*GP*AP*A)-3')
K: Capsid protein
i: RNA (5'-R(P*GP*AP*A)-3')
L: Capsid protein
j: RNA (5'-R(P*GP*AP*A)-3')
M: Capsid protein
k: RNA (5'-R(P*GP*AP*A)-3')
N: Capsid protein
l: RNA (5'-R(P*GP*AP*A)-3')
O: Capsid protein
m: RNA (5'-R(P*GP*AP*A)-3')
P: Capsid protein
n: RNA (5'-R(P*GP*AP*A)-3')
Q: Capsid protein
o: RNA (5'-R(P*GP*AP*A)-3')
S: Capsid protein
p: RNA (5'-R(P*GP*AP*A)-3')
T: Capsid protein
q: RNA (5'-R(P*GP*AP*A)-3')
U: Capsid protein
r: RNA (5'-R(P*GP*AP*A)-3')
V: Capsid protein
s: RNA (5'-R(P*GP*AP*A)-3')
W: Capsid protein
t: RNA (5'-R(P*GP*AP*A)-3')
X: Capsid protein
u: RNA (5'-R(P*GP*AP*A)-3')
Y: Capsid protein
v: RNA (5'-R(P*GP*AP*A)-3')


Theoretical massNumber of molelcules
Total (without water)433,21648
Polymers433,21648
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "A"
d_2ens_1chain "B"
d_3ens_1chain "C"
d_4ens_1chain "D"
d_5ens_1chain "E"
d_6ens_1chain "F"
d_7ens_1chain "G"
d_8ens_1chain "H"
d_9ens_1chain "I"
d_10ens_1chain "J"
d_11ens_1chain "U"
d_12ens_1chain "L"
d_13ens_1chain "M"
d_14ens_1chain "N"
d_15ens_1chain "O"
d_16ens_1chain "P"
d_17ens_1chain "Q"
d_18ens_1chain "S"
d_19ens_1chain "T"
d_20ens_1chain "K"
d_21ens_1chain "V"
d_22ens_1chain "W"
d_23ens_1chain "X"
d_24ens_1chain "Y"
d_1ens_2chain "b"
d_2ens_2chain "Z"
d_3ens_2chain "a"
d_4ens_2chain "u"
d_5ens_2chain "R"
d_6ens_2chain "d"
d_7ens_2chain "e"
d_8ens_2chain "f"
d_9ens_2chain "g"
d_10ens_2chain "h"
d_11ens_2chain "i"
d_12ens_2chain "j"
d_13ens_2chain "c"
d_14ens_2chain "l"
d_15ens_2chain "m"
d_16ens_2chain "n"
d_17ens_2chain "o"
d_18ens_2chain "p"
d_19ens_2chain "q"
d_20ens_2chain "r"
d_21ens_2chain "s"
d_22ens_2chain "t"
d_23ens_2chain "k"
d_24ens_2chain "v"

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1SERTHRA1 - 153
d_21ens_1SERTHRB1 - 153
d_31ens_1SERTHRC1 - 153
d_41ens_1SERTHRD1 - 153
d_51ens_1SERTHRE1 - 153
d_61ens_1SERTHRF1 - 153
d_71ens_1SERTHRG1 - 153
d_81ens_1SERTHRH1 - 153
d_91ens_1SERTHRI1 - 153
d_101ens_1SERTHRJ1 - 153
d_111ens_1SERTHRK1 - 153
d_121ens_1SERTHRL1 - 153
d_131ens_1SERTHRM1 - 153
d_141ens_1SERTHRN1 - 153
d_151ens_1SERTHRO1 - 153
d_161ens_1SERTHRP1 - 153
d_171ens_1SERTHRQ1 - 153
d_181ens_1SERTHRR1 - 153
d_191ens_1SERTHRS1 - 153
d_201ens_1SERTHRT1 - 153
d_211ens_1SERTHRU1 - 153
d_221ens_1SERTHRV1 - 153
d_231ens_1SERTHRW1 - 153
d_241ens_1SERTHRX1 - 153
d_11ens_2GAY
d_21ens_2GAZ
d_31ens_2GAAA
d_41ens_2GABA
d_51ens_2GACA
d_61ens_2GADA
d_71ens_2GAEA
d_81ens_2GAFA
d_91ens_2GAGA
d_101ens_2GAHA
d_111ens_2GAIA
d_121ens_2GAJA
d_131ens_2GAKA
d_141ens_2GALA
d_151ens_2GAMA
d_161ens_2GANA
d_171ens_2GAOA
d_181ens_2GAPA
d_191ens_2GAQA
d_201ens_2GARA
d_211ens_2GASA
d_221ens_2GATA
d_231ens_2GAUA
d_241ens_2GAVA

NCS ensembles :
ID
ens_1
ens_2

NCS oper:
IDCodeMatrixVector
1given(-0.67237404574, 0.740211190054, 0.000732622094915), (-0.740211149098, -0.672374383329, 0.000378674559879), (0.000772895475893, -0.000287684096886, 0.999999659935)178.828892636, 463.311431758, -8.5565545842
2given(-0.761888364266, -0.647707967859, 0.000713280086457), (0.647707478817, -0.761888682193, -0.000811068398746), (0.00106877548945, -0.000155946729139, 0.9999994167)462.660602548, 214.18413971, -14.2841733183
3given(0.718685880154, -0.695334845767, -0.000240685757152), (0.695334833764, 0.718685917625, -0.000144090444822), (0.000273168571465, 0.999999960654)187.646261345, -79.4960634779, 2.78820790826
4given(0.283119788015, 0.959084248233, 0.000768391071686), (-0.95908447517, 0.283119344271, 0.000637484969622), (0.000393855416491, -0.000917436557176, 0.999999501594)-46.6785974882, 321.758266483, 18.4550154999
5given(-0.345172437407, 0.938539276004, 0.000125907532368), (-0.93853921152, -0.345172460365, 0.000347915099701), (0.00036999179851, 0.999999931551)78.0379521611, 438.543983342, -7.12108195351
6given(0.92697783649, 0.375116016655, -0.00025437127419), (-0.375115826961, 0.926977763975, 0.000584345551189), (0.00045499389048, -0.000446256683931, 0.999999796918)-57.9838303854, 85.9633894259, -1.41085730956
7given(-0.46413011639, 0.885766832113, 0.00059513700933), (-0.885767002617, -0.464130169405), (0.000228329836315, -0.000552247083348, 0.999999821444)110.935180295, 451.350032089, 15.5991651608
8given(-0.90036667483, -0.435131903318, 0.000278515367306), (0.435131838647, -0.900366710311, -0.000264498021693), (0.000365857492635, -0.000116954300423, 0.999999926235)448.500706314, 281.516345076, 9.83888944146
9given(-0.900835061899, 0.43416060926, 0.000869839213042), (-0.434160979614, -0.900835258076, -0.000285634199501), (0.000659570713885, -0.000634959546629, 0.999999580896)281.502025209, 448.536828918, -9.89964041314
10given(-0.949050081819, -0.315124573769, 0.000667238508896), (0.315124410055, -0.949050309663, -0.000340464727649), (0.00074053171567, -0.000112854936149, 0.999999719438)434.717159702, 313.93863409, -12.8300911073
11given(-0.345058190279, -0.93858128522, -0.000127888707009), (0.938581003832, -0.345057973293, -0.000833253928421), (0.000737947525004, -0.000407555003588, 0.999999644666)438.630821534, 78.2371956335, 7.00211393363
12given(0.718318041787, 0.695714876569), (-0.695714876137, 0.718318035934, 0.000101848939081), (0.999999994131)-79.5403283222, 187.670615711, -2.8183338692
13given(-0.671462594081, -0.741038445575), (0.741038350433, -0.671462557706, -0.00044360647746), (0.000384626570357, -0.000236176077466, 0.999999898142)463.337110596, 178.816884676, 8.45034516437
14given(-0.762086596386, 0.647474385533, 0.0009693752662), (-0.647474778557, -0.762086868357, -0.000127323050752), (0.000656309746831, -0.00072467722621, 0.99999952205)213.89066401, 462.831560717, 14.1333550257
15given(-0.99789816009, 0.0647976458713, 0.000726070978426), (-0.0647979129603, -0.997898345319, -0.000350551508645), (0.000701830115438, -0.000396862589557, 0.999999674967)371.116455538, 396.259630578, -11.36736699
16given(0.0327183919687, -0.99946460939), (0.999464472209, 0.0327183677432, -0.000526502447993), (0.000524994851252, 0.999999860696)377.75507025, -6.05230455898, 5.54903743562
17given(0.405056316784, 0.91429169988, 0.000260315729573), (-0.91429166659, 0.405056157509, 0.000507610561819), (0.000358661634283, -0.000443615366762, 0.999999837284)-61.4061592173, 289.771681086, -4.22296604979
18given(-0.949309757379, 0.314340945183, 0.000868750304881), (-0.314341355505, -0.949309942397, -0.000381426183659), (0.000704815434795, -0.000635175746299, 0.999999549893)313.845242906, 434.850149218, 12.7038294249
19given(0.92702137216, -0.375008366893, -0.000316732775918), (0.375008434407, 0.927021379775, 0.000188585468494), (0.000222896926391, -0.000293600222195, 0.999999932058)86.1281862923, -58.0395705612, 1.42318372008
20given(0.405161056555, -0.914245306784, -0.00019306798376), (0.914245213754, 0.405161102447, -0.000412542737043), (0.000455388898337, 0.999999896267)289.893318261, -61.2626838979, 4.16177676328
21given(-0.0972841870316, 0.995256069882, 0.00106879222863), (-0.995256400775, -0.0972848575928, 0.000594305843162), (0.000695463797532, -0.00100590574584, 0.999999252242)19.3841013696, 401.79070151, 17.0139345746
22given(-0.997856027479, -0.0654441466775, 0.000641941607271), (0.0654435872929, -0.997855902798, -0.000856815639205), (0.000696638790434, -0.000812967688407, 0.999999426889)396.14916198, 371.334418984, 11.3168458694
23given(0.0319722271253, 0.999488201121, 0.00105475675097), (-0.999488530829, 0.0319714910929, 0.000707458998697), (0.000673374775906, -0.0010768363152, 0.999999193495)-6.26326546223, 377.749246735, -5.57830827112
24given(-0.997918842226, 0.0644803226652, 0.000521842009761), (-0.0644802173383, -0.99791896205, 0.000216222489135), (0.000534698132602, 0.000182124009815, 0.999999840464)371.19914236, 396.079960099, -11.4297609116
25given(-0.0971883854828, -0.995265852239, 0.000548720838405), (0.995265801525, -0.0971887022328, -0.000583501159217), (0.000634068244683, 0.000489413549456, 0.999999679216)401.775820601, 19.7022777924, -17.1530881248
26given(-0.671645194983, -0.740872857827, 0.00037495532047), (0.740872488081, -0.671645254556, -0.000780023744807), (0.000829735382653, -0.000246105118977, 0.999999625486)463.281795519, 178.923729926, 8.35377336217
27given(0.718698620206, 0.69532167004, 0.000261699985863), (-0.695321681355, 0.718698654416), (-0.000229676487853, -0.000138974241252, 0.999999963967)-79.5812562509, 187.604525516, -2.75731572099
28given(-0.900669984667, 0.43450382974), (-0.434503829488, -0.900669976945, 0.000121614577422), (0.000120712916405, 0.999999992274)281.58632386, 448.475334038, -9.90677814111
29given(0.405398412289, 0.914140101839), (-0.914140001682, 0.405398387199, 0.000452747608119), (0.000429694839578, -0.000147870149192, 0.999999896748)-61.3796551682, 289.713271347, -4.29174266358
30given(-0.949465911793, 0.313869908046, 0.000403937989147), (-0.313870019727, -0.949465927885, -0.000250005586804), (0.000305056127132, -0.00036415580705, 0.999999887166)314.048860318, 434.764731066, 12.7206589656
31given(-0.344537077237, -0.938772707209), (0.938772700058, -0.344537085086, -0.000120935993168), (0.000141541117093, 0.999999989383)438.517307918, 77.980895273, 7.0246401587
32given(-0.94931022733, -0.314340147046, 0.000603524924002), (0.314339996414, -0.949310410822, -0.000332505914432), (0.000677452451582, -0.000125939242771, 0.999999762599)434.640698544, 314.085272454, -12.828641884
33given(0.926997606123, 0.375067240284), (-0.375067218584, 0.926997588596, 0.000228651006238), (0.000140663448132, -0.000189744562204, 0.999999972105)-57.9967663337, 86.0320660131, -1.40909698689
34given(0.404636781499, -0.914477469077, -0.000183327200207), (0.914477301677, 0.40463681895, -0.000556297186788), (0.000582902178548, 0.999999828462)290.009755033, -61.1914376564, 4.11340556135
35given(-0.463393154184, 0.886152456184, 0.000780419464056), (-0.88615251787, -0.463393529584, 0.000389631324633), (0.000706914085334, -0.000511018184577, 0.999999619566)110.717403781, 451.192163809, 15.4889976646
36given(0.0326803799897, -0.999465851386), (0.999465828164, 0.0326803945527, -0.000223929101237), (0.000226044448054, 0.999999972589)377.754539839, -6.12118624356, 5.61810184633
37given(-0.997913319594, -0.0645636897486, 0.000732491587183), (0.0645635302547, -0.997913570038, -0.000239362120988), (0.000746417396506, -0.00019157040599, 0.999999703081)396.005696229, 371.384021706, 11.1857898771
38given(-0.762229834104, -0.647306321467, 0.000454083616379), (0.647306254314, -0.762229966676, -0.000301707495002), (0.000541413308529, 0.99999985139)462.685926972, 214.200624363, -14.2532501298
39given(0.71847054809, -0.695557372852, 0.00011223952176), (0.695557373275, 0.718470504393, -0.000273497241131), (0.000109592236711, 0.00027456873967, 0.999999956301)187.643541783, -79.4499208549, 2.75865796104
40given(-0.344611739796, 0.938745263455, 0.000281309449359), (-0.9387452505, -0.344611806771, 0.000239371227873), (0.000321651163978, -0.000181587774212, 0.999999931783)77.8889937996, 438.521893649, -7.09318600816
41given(-0.900832658993, -0.434166069648, 0.000586905847689), (0.434165893796, -0.900832843322, -0.000406271724093), (0.000705093461195, -0.000111168335552, 0.999999745242)448.365260115, 281.770008118, 9.7745494265
42given(-0.462958352484, -0.886379924636, 0.000439393774272), (0.886379948734, -0.462958468156, -0.000207952104709), (0.000387745639554, 0.000293196667322, 0.999999881845)451.121645151, 110.814271774, -15.6685778749
43given(0.926884707264, -0.375346156696), (0.375346143068, 0.92688469589, -0.000182778624592), (0.000111014031474, 0.000152241086117, 0.999999982249)86.1358715475, -58.0106789502, 1.36783502515
44given(-0.761950542166, 0.647634469774, 0.000982269098409), (-0.647634497223, -0.761951079284, 0.000332842687873), (0.000964001397359, -0.000382541687205, 0.999999462181)213.84142924, 462.742318141, 14.0141619302
45given(0.0324960920217, 0.999471848137, 0.000169661208671), (-0.999471855967, 0.032496110803, -0.000109140252547), (-0.000114595939355, -0.000166024971426, 0.999999979652)-6.18081833526, 377.795119124, -5.60251941815
46given(-0.671981962533, 0.740566821332, 0.00101250775592), (-0.740566825655, -0.671982573757, 0.00044419170188), (0.0010093412045, -0.000451340843184, 0.999999388761)178.694321933, 463.285986557, -8.59159590999

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Components

#1: Protein ...
Capsid protein


Mass: 17091.998 Da / Num. of mol.: 24 / Source method: isolated from a natural source / Source: (natural) Tobacco mosaic virus / References: UniProt: A0A348G6U1
#2: RNA chain ...
RNA (5'-R(P*GP*AP*A)-3')


Mass: 958.660 Da / Num. of mol.: 24 / Source method: isolated from a natural source / Source: (natural) Tobacco mosaic virus

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: HELICAL ARRAY / 3D reconstruction method: helical reconstruction

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Sample preparation

ComponentName: Tobacco mosaic virus / Type: VIRUS / Entity ID: all / Source: NATURAL
Source (natural)Organism: Tobacco mosaic virus
Details of virusEmpty: NO / Enveloped: NO / Isolate: OTHER / Type: VIRION
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 500 nm
Image recordingElectron dose: 40 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

Software
NameVersionClassificationNB
phenix.real_space_refine1.20.1_4487refinement
PHENIX1.20.1_4487refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Helical symmertyAngular rotation/subunit: 22.04 ° / Axial rise/subunit: 1.41 Å / Axial symmetry: C1
3D reconstructionResolution: 2.3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 109763 / Symmetry type: HELICAL
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 28.06 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.001631320
ELECTRON MICROSCOPYf_angle_d0.451143104
ELECTRON MICROSCOPYf_chiral_restr0.03725112
ELECTRON MICROSCOPYf_plane_restr0.00355328
ELECTRON MICROSCOPYf_dihedral_angle_d7.45674800
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AELECTRON MICROSCOPYNCS constraints1.09658083134E-10
ens_1d_3AELECTRON MICROSCOPYNCS constraints2.25101369363E-13
ens_1d_4AELECTRON MICROSCOPYNCS constraints6.55693229472E-12
ens_1d_5AELECTRON MICROSCOPYNCS constraints3.46845188094E-10
ens_1d_6AELECTRON MICROSCOPYNCS constraints7.43064184775E-11
ens_1d_7AELECTRON MICROSCOPYNCS constraints3.29340712157E-10
ens_1d_8AELECTRON MICROSCOPYNCS constraints3.01381506443E-11
ens_1d_9AELECTRON MICROSCOPYNCS constraints2.29199556291E-12
ens_1d_10AELECTRON MICROSCOPYNCS constraints1.25183574297E-13
ens_1d_11AELECTRON MICROSCOPYNCS constraints2.27081598098E-11
ens_1d_12AELECTRON MICROSCOPYNCS constraints1.28315028621E-10
ens_1d_13AELECTRON MICROSCOPYNCS constraints3.45278051086E-12
ens_1d_14AELECTRON MICROSCOPYNCS constraints2.40626823573E-11
ens_1d_15AELECTRON MICROSCOPYNCS constraints4.58414589532E-10
ens_1d_16AELECTRON MICROSCOPYNCS constraints2.74633461157E-13
ens_1d_17AELECTRON MICROSCOPYNCS constraints1.63048103486E-11
ens_1d_18AELECTRON MICROSCOPYNCS constraints5.73491580595E-11
ens_1d_19AELECTRON MICROSCOPYNCS constraints1.98876200762E-13
ens_1d_20AELECTRON MICROSCOPYNCS constraints4.34435108069E-11
ens_1d_21AELECTRON MICROSCOPYNCS constraints1.51096427546E-11
ens_1d_22AELECTRON MICROSCOPYNCS constraints1.40548711137E-10
ens_1d_23AELECTRON MICROSCOPYNCS constraints2.91074889111E-11
ens_1d_24AELECTRON MICROSCOPYNCS constraints1.2852235065E-10
ens_2d_2HELECTRON MICROSCOPYNCS constraints6.9009891022E-14
ens_2d_3HELECTRON MICROSCOPYNCS constraints4.10609035098E-13
ens_2d_4HELECTRON MICROSCOPYNCS constraints4.6189949582E-11
ens_2d_5HELECTRON MICROSCOPYNCS constraints1.16622849431E-11
ens_2d_6HELECTRON MICROSCOPYNCS constraints6.5606504887E-14
ens_2d_7HELECTRON MICROSCOPYNCS constraints1.86825968722E-11
ens_2d_8HELECTRON MICROSCOPYNCS constraints2.46006873134E-11
ens_2d_9HELECTRON MICROSCOPYNCS constraints8.88623366766E-11
ens_2d_10HELECTRON MICROSCOPYNCS constraints7.71465274105E-12
ens_2d_11HELECTRON MICROSCOPYNCS constraints4.33622301185E-12
ens_2d_12HELECTRON MICROSCOPYNCS constraints2.5696702723E-11
ens_2d_13HELECTRON MICROSCOPYNCS constraints4.4831223729E-13
ens_2d_14HELECTRON MICROSCOPYNCS constraints9.3841221392E-12
ens_2d_15HELECTRON MICROSCOPYNCS constraints7.748675345E-14
ens_2d_16HELECTRON MICROSCOPYNCS constraints1.82120187586E-13
ens_2d_17HELECTRON MICROSCOPYNCS constraints6.13532326588E-12
ens_2d_18HELECTRON MICROSCOPYNCS constraints5.82147476819E-12
ens_2d_19HELECTRON MICROSCOPYNCS constraints2.84625521993E-12
ens_2d_20HELECTRON MICROSCOPYNCS constraints1.33847390546E-11
ens_2d_21HELECTRON MICROSCOPYNCS constraints5.7382151043E-12
ens_2d_22HELECTRON MICROSCOPYNCS constraints6.01343675216E-13
ens_2d_23HELECTRON MICROSCOPYNCS constraints6.12943786904E-12
ens_2d_24HELECTRON MICROSCOPYNCS constraints6.38198049532E-13

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