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- PDB-6rh1: Revisiting pH-gated conformational switch. Complex HK853-RR468 D5... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6rh1 | ||||||||||||||||||
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Title | Revisiting pH-gated conformational switch. Complex HK853-RR468 D53A pH 7 | ||||||||||||||||||
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![]() | SIGNALING PROTEIN / Histidine Kinase / Response Regulator / Phosphotransfer / Phosphatase | ||||||||||||||||||
Function / homology | ![]() histidine phosphotransfer kinase activity / histidine kinase / phosphorelay signal transduction system / phosphorelay sensor kinase activity / nucleotide binding / identical protein binding / metal ion binding Similarity search - Function | ||||||||||||||||||
Biological species | ![]() ![]() | ||||||||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||||||||
![]() | Mideros-Mora, C. / Casino, P. / Marina, A. | ||||||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases. Authors: Mideros-Mora, C. / Miguel-Romero, L. / Felipe-Ruiz, A. / Casino, P. / Marina, A. | ||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 162.5 KB | Display | ![]() |
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PDB format | ![]() | 125.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 31.5 KB | Display | |
Data in CIF | ![]() | 44.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6rfvC ![]() 6rgyC ![]() 6rgzC ![]() 6rh0C ![]() 6rh2C ![]() 6rh7C ![]() 6rh8C ![]() 3dgeS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29378.281 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 13804.245 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | #4: Chemical | ChemComp-SO4 / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.17 Å3/Da / Density % sol: 61.17 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: 1,8 M NH4SO4, citrato pH 7 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 21, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97926 Å / Relative weight: 1 |
Reflection | Resolution: 2→87.9 Å / Num. obs: 71301 / % possible obs: 98 % / Redundancy: 5.7 % / CC1/2: 0.998 / Net I/σ(I): 10.4 |
Reflection shell | Resolution: 2→2.04 Å / Num. unique obs: 4543 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB 3DGE Resolution: 2→87.9 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.939 / SU B: 5.552 / SU ML: 0.146 / Cross valid method: THROUGHOUT / ESU R: 0.16 / ESU R Free: 0.155 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.632 Å2
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Refinement step | Cycle: LAST / Resolution: 2→87.9 Å
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Refine LS restraints |
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