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- PDB-6ous: Structure of fusion glycoprotein from human respiratory syncytial... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ous | ||||||||||||
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Title | Structure of fusion glycoprotein from human respiratory syncytial virus | ||||||||||||
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![]() | VIRAL PROTEIN/Immune system / Antibody / RSV / Neutralizing / VIRAL PROTEIN / VIRAL PROTEIN-Immune system complex | ||||||||||||
Function / homology | ![]() positive regulation of syncytium formation by virus / Translation of respiratory syncytial virus mRNAs / RSV-host interactions / Maturation of hRSV A proteins / Assembly and release of respiratory syncytial virus (RSV) virions / host cell Golgi membrane / Respiratory syncytial virus (RSV) attachment and entry / entry receptor-mediated virion attachment to host cell / symbiont entry into host cell / fusion of virus membrane with host plasma membrane ...positive regulation of syncytium formation by virus / Translation of respiratory syncytial virus mRNAs / RSV-host interactions / Maturation of hRSV A proteins / Assembly and release of respiratory syncytial virus (RSV) virions / host cell Golgi membrane / Respiratory syncytial virus (RSV) attachment and entry / entry receptor-mediated virion attachment to host cell / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / viral envelope / host cell plasma membrane / virion membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() ![]() | ||||||||||||
![]() | Su, H.P. | ||||||||||||
![]() | ![]() Title: A potent broadly neutralizing human RSV antibody targets conserved site IV of the fusion glycoprotein. Authors: Tang, A. / Chen, Z. / Cox, K.S. / Su, H.P. / Callahan, C. / Fridman, A. / Zhang, L. / Patel, S.B. / Cejas, P.J. / Swoyer, R. / Touch, S. / Citron, M.P. / Govindarajan, D. / Luo, B. / Eddins, ...Authors: Tang, A. / Chen, Z. / Cox, K.S. / Su, H.P. / Callahan, C. / Fridman, A. / Zhang, L. / Patel, S.B. / Cejas, P.J. / Swoyer, R. / Touch, S. / Citron, M.P. / Govindarajan, D. / Luo, B. / Eddins, M. / Reid, J.C. / Soisson, S.M. / Galli, J. / Wang, D. / Wen, Z. / Heidecker, G.J. / Casimiro, D.R. / DiStefano, D.J. / Vora, K.A. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.9 MB | Display | ![]() |
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PDB format | ![]() | 1.6 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.3 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 175 KB | Display | |
Data in CIF | ![]() | 236.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Fusion glycoprotein ... , 2 types, 12 molecules ACEGIKBDFHJL
#1: Protein | Mass: 9512.768 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Protein | Mass: 45609.148 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: A2 / Production host: ![]() |
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-Antibody , 2 types, 12 molecules MOQSUWNPRTVX
#3: Antibody | Mass: 24551.439 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Antibody | Mass: 23406.043 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 2 types, 10 molecules ![](data/chem/img/NAG.gif)
#5: Polysaccharide | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #6: Sugar | ChemComp-NAG / |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.37 Å3/Da / Density % sol: 63.51 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 100 mM Tris 8.5, 10% PEG 8000, 200 mM ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: May 17, 2015 | |||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 | |||||||||||||||||||||||||||
Reflection | Resolution: 3.4→49.86 Å / Num. obs: 114498 / % possible obs: 99 % / Redundancy: 2 % / Biso Wilson estimate: 78.54 Å2 / CC1/2: 0.98 / Rmerge(I) obs: 0.127 / Rpim(I) all: 0.127 / Rrim(I) all: 0.179 / Net I/σ(I): 3.9 / Num. measured all: 228565 | |||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1 / Redundancy: 2 %
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-Phasing
Phasing | Method: ![]() | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4mmt, 1hzh Resolution: 3.4→49.86 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 3.4→49.86 Å
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