BIOSYNTHETIC PROTEIN / modulator for quorum sensing in streptococcus pneumoniae
機能・相同性
Competence protein, ComC / COMC family / pheromone activity / establishment of competence for transformation / extracellular region / Competence-stimulating peptide type 1
分子量: 2218.689 Da / 分子数: 1 / 変異: K6(DPP), D10E / 由来タイプ: 合成 詳細: DPP and GLU were chemically added to the 6th and 10th positions chemically and then cyclized. However, during structure calculation, GLU10 was labeled as GLN10 to construct the macrocycle for ...詳細: DPP and GLU were chemically added to the 6th and 10th positions chemically and then cyclized. However, during structure calculation, GLU10 was labeled as GLN10 to construct the macrocycle for convenience. GLN10 was kept in the final coordinate file and chemical shift file for consistency. The actual sequence is still: EMRLS(DPP)FFR(GLU)FILQRKK 由来: (合成) Streptococcus pneumoniae (肺炎レンサ球菌) 参照: UniProt: P60242
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
タイプ
1
1
1
isotropic
1
2D 1H-1H COSY
1
2
1
isotropic
1
2D 1H-1H TOCSY
1
3
1
isotropic
1
2D 1H-1H NOESY
1
4
1
isotropic
1
2D 1H-15N HSQC
1
5
1
isotropic
1
2D 1H-13C HSQC
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試料調製
詳細
タイプ: solution 内容: 1.9 mM CSP1-cyc(Dap6E10), 250 mM Deuterium DPC, 137 mM sodium chloride, 2.7 mM potassium chloride, 10 mM Na2HPO4, 1.8 mM na KH2PO4, 90% H2O/10% D2O Label: 1 / 溶媒系: 90% H2O/10% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
1.9mM
CSP1-cyc(Dap6E10)
naturalabundance
1
250mM
DPC
Deuterium
1
137mM
sodiumchloride
naturalabundance
1
2.7mM
potassiumchloride
naturalabundance
1
10mM
Na2HPO4
naturalabundance
1
1.8mM
KH2PO4
na
1
試料状態
イオン強度: 0.15 M / Label: 1 / pH: 7.4 / 圧: 1 atm / 温度: 298 K
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NMR測定
NMRスペクトロメーター
タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 900 MHz