PDB-6m7z: A divergent kinase lacking the glycine-rich loop regulates membra...

Basic information

• Entry: Database: PDB / ID: 6m7z
• Title: A divergent kinase lacking the glycine-rich loop regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuole
• Components: Bradyzoite pseudokinase 1
• Keywords: TRANSFERASE / kinase / atypical / SIGNALING PROTEIN
• Function / homology: cyst wall / protein kinase activity / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / : / ATP binding / Bradyzoite pseudokinase 1
• Biological species: Toxoplasma gondii (eukaryote)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / SAD / MAD / Resolution: 2.5 Å
• Authors: Beraki, T. / Borek, D.M. / Reese, M.L.

Funding support

United States, 2items

Organization	Grant number	Country
National Science Foundation (NSF, United States)	MCB1553334	United States
Robert A. Welch Foundation	I-1936-20170325	United States

• Citation: Journal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2019; Title: Divergent kinase regulates membrane ultrastructure of theToxoplasmaparasitophorous vacuole.; Authors: Beraki, T. / Hu, X. / Broncel, M. / Young, J.C. / O'Shaughnessy, W.J. / Borek, D. / Treeck, M. / Reese, M.L.

History

Deposition	Aug 21, 2018	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Oct 17, 2018	Provider: repository / Type: Initial release
Revision 1.1	Mar 20, 2019	Group: Data collection / Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Apr 10, 2019	Group: Data collection / Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3	Nov 27, 2019	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4	Mar 13, 2024	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / struct_ncs_dom_lim Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

Assembly

Deposited unit

A: Bradyzoite pseudokinase 1

B: Bradyzoite pseudokinase 1

C: Bradyzoite pseudokinase 1

D: Bradyzoite pseudokinase 1

E: Bradyzoite pseudokinase 1

F: Bradyzoite pseudokinase 1

hetero molecules

Summary:

• 217 kDa, 6 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	216,734	25
Polymers	215,635	6
Non-polymers	1,099	19
Water	6,864	381

1

A: Bradyzoite pseudokinase 1

hetero molecules

Summary:

• defined by software
• Evidence: software-determined (PISA v1.52)
• 36.1 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	36,063	3
Polymers	35,939	1
Non-polymers	124	2
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

2

B: Bradyzoite pseudokinase 1

hetero molecules

Summary:

• defined by software
• 36.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	36,187	5
Polymers	35,939	1
Non-polymers	248	4
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

3

C: Bradyzoite pseudokinase 1

hetero molecules

Summary:

• defined by software
• 36.3 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	36,347	8
Polymers	35,939	1
Non-polymers	408	7
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

4

D: Bradyzoite pseudokinase 1

Summary:

• defined by software
• 35.9 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,939	1
Polymers	35,939	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

5

E: Bradyzoite pseudokinase 1

hetero molecules

Summary:

• defined by software
• 36.1 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	36,134	5
Polymers	35,939	1
Non-polymers	195	4
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

6

F: Bradyzoite pseudokinase 1

hetero molecules

Summary:

• defined by software
• 36.1 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	36,063	3
Polymers	35,939	1
Non-polymers	124	2
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

Unit cell

Length a, b, c (Å)	171.466, 123.068, 86.615
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	18
Space group name H-M	P21212

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
1	1	A
2	1	B
1	2	A
2	2	C
1	3	A
2	3	D
1	4	A
2	4	E
1	5	A
2	5	F
1	6	B
2	6	C
1	7	B
2	7	D
1	8	B
2	8	E
1	9	B
2	9	F
1	10	C
2	10	D
1	11	C
2	11	E
1	12	C
2	12	F
1	13	D
2	13	E
1	14	D
2	14	F
1	15	E
2	15	F

NCS domain segments:

Component-ID: _ / Refine code: _

Dom-ID	Ens-ID	Beg auth comp-ID	Beg label comp-ID	End auth comp-ID	End label comp-ID	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	ALA	ALA	ARG	ARG	A	A	68 - 371	10 - 313
2	1	ALA	ALA	ARG	ARG	B	B	68 - 371	10 - 313
1	2	ALA	ALA	TYR	TYR	A	A	68 - 372	10 - 314
2	2	ALA	ALA	TYR	TYR	C	C	68 - 372	10 - 314
1	3	THR	THR	TYR	TYR	A	A	71 - 372	13 - 314
2	3	THR	THR	TYR	TYR	D	D	71 - 372	13 - 314
1	4	ALA	ALA	ARG	ARG	A	A	68 - 371	10 - 313
2	4	ALA	ALA	ARG	ARG	E	E	68 - 371	10 - 313
1	5	ALA	ALA	TYR	TYR	A	A	68 - 372	10 - 314
2	5	ALA	ALA	TYR	TYR	F	F	68 - 372	10 - 314
1	6	PRO	PRO	ARG	ARG	B	B	66 - 371	8 - 313
2	6	PRO	PRO	ARG	ARG	C	C	66 - 371	8 - 313
1	7	THR	THR	ARG	ARG	B	B	71 - 371	13 - 313
2	7	THR	THR	ARG	ARG	D	D	71 - 371	13 - 313
1	8	ILE	ILE	ARG	ARG	B	B	65 - 371	7 - 313
2	8	ILE	ILE	ARG	ARG	E	E	65 - 371	7 - 313
1	9	ILE	ILE	ARG	ARG	B	B	65 - 371	7 - 313
2	9	ILE	ILE	ARG	ARG	F	F	65 - 371	7 - 313
1	10	THR	THR	TYR	TYR	C	C	71 - 372	13 - 314
2	10	THR	THR	TYR	TYR	D	D	71 - 372	13 - 314
1	11	PRO	PRO	TYR	TYR	C	C	66 - 372	8 - 314
2	11	PRO	PRO	TYR	TYR	E	E	66 - 372	8 - 314
1	12	PRO	PRO	TYR	TYR	C	C	66 - 372	8 - 314
2	12	PRO	PRO	TYR	TYR	F	F	66 - 372	8 - 314
1	13	THR	THR	TYR	TYR	D	D	71 - 372	13 - 314
2	13	THR	THR	TYR	TYR	E	E	71 - 372	13 - 314
1	14	THR	THR	TYR	TYR	D	D	71 - 372	13 - 314
2	14	THR	THR	TYR	TYR	F	F	71 - 372	13 - 314
1	15	ILE	ILE	TYR	TYR	E	E	65 - 372	7 - 314
2	15	ILE	ILE	TYR	TYR	F	F	65 - 372	7 - 314

NCS ensembles :

ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15

• Details: THE AUTHOR STATES THAT THE BIOLOGICAL UNIT OF THIS PROTEIN IS UNKNOWN.

Components

#1: Protein

A B C D E F
Bradyzoite pseudokinase 1 / BPK1

Details:

Mass: 35939.133 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Toxoplasma gondii (eukaryote) / Strain: RH / Gene: BPK1 / Plasmid: pGEX4T / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21/Rosetta2 / References: UniProt: A0A452CSY6*PLUS, EC: 2.7.99.1

#2: Chemical

A-401 A-402 B-401 B-402 B-403 B-404 C-401 C-402 C-403 C-404 C-405 C-406 E-401 E-402 F-401 F-402
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL

Details:

Mass: 62.068 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: C₂H₆O₂

#3: Chemical

C-407 E-403 E-404 ChemComp-CL / CHLORIDE ION

Details:

Mass: 35.453 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Formula: Cl

#4: Water

ChemComp-HOH / water

Details:

Mass: 18.015 Da / Num. of mol.: 381 / Source method: isolated from a natural source / Formula: H₂O

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.12 ų/Da / Density % sol: 41.95 % / Mosaicity: 0.569 °
• Crystal grow: Temperature: 298 K / Method: vapor diffusion / pH: 7.4 / Details: 0.2M Proline, 0.1M HEPES 7.4, 10% PEG-3350

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 1.038 Å
• Detector: Type: ADSC QUANTUM 315r / Detector: CCD / Date: Aug 12, 2015
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1.038 Å / Relative weight: 1
• Reflection: Resolution: 2.5→50 Å / Num. obs: 64181 / % possible obs: 99.9 % / Redundancy: 8 % / R_merge(I) obs: 0.088 / R_pim(I) all: 0.034 / R_rim(I) all: 0.095 / Χ²: 1.003 / Net I/σ(I): 9.5 / Num. measured all: 512518

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	Rmerge(I) obs	Num. unique obs	CC1/2	Rpim(I) all	Rrim(I) all	Χ2	% possible all
2.5-2.54	7.3	0.855	3158	0.761	0.345	0.925	0.788	99.8
2.54-2.59	7.6	0.738	3141	0.803	0.294	0.796	0.762	99.7
2.59-2.64	7.8	0.68	3182	0.852	0.266	0.731	0.774	100
2.64-2.69	7.9	0.645	3161	0.827	0.25	0.693	0.855	100
2.69-2.75	8.1	0.544	3156	0.926	0.207	0.583	0.788	100
2.75-2.82	8.2	0.461	3207	0.939	0.175	0.493	0.802	100
2.82-2.89	8.2	0.378	3176	0.955	0.143	0.404	0.805	100
2.89-2.96	8.2	0.308	3170	0.965	0.116	0.329	0.812	100
2.96-3.05	8.2	0.266	3164	0.977	0.1	0.284	0.831	100
3.05-3.15	8.2	0.188	3198	0.987	0.071	0.201	0.843	100
3.15-3.26	8.2	0.147	3191	0.991	0.055	0.157	0.9	100
3.26-3.39	8.2	0.118	3176	0.994	0.044	0.126	0.964	100
3.39-3.55	8.2	0.105	3200	0.992	0.04	0.112	1.136	100
3.55-3.73	8.2	0.091	3223	0.994	0.034	0.097	1.314	100
3.73-3.97	8.1	0.08	3207	0.995	0.03	0.085	1.405	100
3.97-4.27	8.1	0.071	3231	0.996	0.026	0.076	1.427	100
4.27-4.7	8	0.069	3238	0.996	0.026	0.073	1.559	100
4.7-5.38	7.9	0.064	3268	0.996	0.024	0.068	1.386	100
5.38-6.78	7.7	0.05	3303	0.997	0.019	0.053	0.873	100
6.78-50	7.4	0.046	3431	0.997	0.018	0.049	0.956	99

Phasing

• Phasing: Method: MAD

Processing

Software

Name	Version	Classification	NB
REFMAC	5.8.0230	refinement
HKL-2000		data reduction
SCALEPACK		data scaling
SHELXDE		phasing
PDB_EXTRACT	3.24	data extraction

Refinement

Method to determine structure: SAD / Resolution: 2.5→47.7 Å / Cor.coef. F_o:F_c: 0.935 / Cor.coef. F_o:F_c free: 0.902 / SU B: 17.847 / SU ML: 0.201 / Cross valid method: THROUGHOUT / ESU R: 0.93 / ESU R Free: 0.302 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

	Rfactor	Num. reflection	% reflection	Selection details
Rfree	0.23877	2987	5.1 %	RANDOM
Rwork	0.19721	-	-	-
obs	0.1993	58472	90.9 %	-

• Solvent computation: Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK

Displacement parameters

B_iso mean: 43.39 Ų

	Baniso -1	Baniso -2	Baniso -3
1-	0.94 Å2	0 Å2	-0 Å2
2-	-	-0.47 Å2	0 Å2
3-	-	-	-0.47 Å2

Refinement step

Cycle: 1 / Resolution: 2.5→47.7 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	12413	0	67	381	12861

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.008	0.014	12717
X-RAY DIFFRACTION	r_bond_other_d	0.001	0.017	11920
X-RAY DIFFRACTION	r_angle_refined_deg	1.244	1.655	17185
X-RAY DIFFRACTION	r_angle_other_deg	0.852	1.63	27958
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	6.374	5	1545
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	29.512	21.153	659
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	14.362	15	2268
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	20.996	15	108
X-RAY DIFFRACTION	r_chiral_restr	0.064	0.2	1590
X-RAY DIFFRACTION	r_gen_planes_refined	0.005	0.02	13941
X-RAY DIFFRACTION	r_gen_planes_other	0.001	0.02	2159
X-RAY DIFFRACTION	r_nbd_refined
X-RAY DIFFRACTION	r_nbd_other
X-RAY DIFFRACTION	r_nbtor_refined
X-RAY DIFFRACTION	r_nbtor_other
X-RAY DIFFRACTION	r_xyhbond_nbd_refined
X-RAY DIFFRACTION	r_xyhbond_nbd_other
X-RAY DIFFRACTION	r_metal_ion_refined
X-RAY DIFFRACTION	r_metal_ion_other
X-RAY DIFFRACTION	r_symmetry_vdw_refined
X-RAY DIFFRACTION	r_symmetry_vdw_other
X-RAY DIFFRACTION	r_symmetry_hbond_refined
X-RAY DIFFRACTION	r_symmetry_hbond_other
X-RAY DIFFRACTION	r_symmetry_metal_ion_refined
X-RAY DIFFRACTION	r_symmetry_metal_ion_other
X-RAY DIFFRACTION	r_mcbond_it	4.079	1.599	6240
X-RAY DIFFRACTION	r_mcbond_other	4.079	1.598	6239
X-RAY DIFFRACTION	r_mcangle_it	6.15	2.369	7759
X-RAY DIFFRACTION	r_mcangle_other	6.15	2.369	7760
X-RAY DIFFRACTION	r_scbond_it	5.359	2.149	6477
X-RAY DIFFRACTION	r_scbond_other	5.358	2.149	6477
X-RAY DIFFRACTION	r_scangle_it
X-RAY DIFFRACTION	r_scangle_other	7.707	2.984	9425
X-RAY DIFFRACTION	r_long_range_B_refined	10.305	19.181	13558
X-RAY DIFFRACTION	r_long_range_B_other	10.311	19.081	13504
X-RAY DIFFRACTION	r_rigid_bond_restr
X-RAY DIFFRACTION	r_sphericity_free
X-RAY DIFFRACTION	r_sphericity_bonded

Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05

Ens-ID	Dom-ID	Auth asym-ID	Number	Rms dev position (Å)
1	1	A	7726	0.09
1	2	B	7726	0.09
2	1	A	7932	0.09
2	2	C	7932	0.09
3	1	A	7783	0.09
3	2	D	7783	0.09
4	1	A	7970	0.08
4	2	E	7970	0.08
5	1	A	7826	0.08
5	2	F	7826	0.08
6	1	B	7739	0.09
6	2	C	7739	0.09
7	1	B	7521	0.1
7	2	D	7521	0.1
8	1	B	7818	0.08
8	2	E	7818	0.08
9	1	B	7735	0.09
9	2	F	7735	0.09
10	1	C	7771	0.09
10	2	D	7771	0.09
11	1	C	7928	0.09
11	2	E	7928	0.09
12	1	C	7811	0.1
12	2	F	7811	0.1
13	1	D	7742	0.09
13	2	E	7742	0.09
14	1	D	7721	0.09
14	2	F	7721	0.09
15	1	E	7892	0.09
15	2	F	7892	0.09

LS refinement shell

Resolution: 2.5→2.59 Å / Total num. of bins used: 20

	Rfactor	Num. reflection	% reflection
Rfree	0.289	155	-
Rwork	0.238	2824	-
obs	-	4024	63.5 %

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	8.0742	1.3123	4.3277	0.2324	0.7853	15.8599	-0.5969	-0.5391	0.0164	-0.1526	-0.0371	-0.0021	-0.636	-0.4739	0.6341	0.4527	0.0394	0.0143	0.2668	-0.0019	0.2832	36.0195	51.9959	23.5461
2	3.266	2.6656	1.1684	9.136	-4.1305	4.1689	0.114	0.1395	0.0146	0.4096	-0.1379	-0.0111	-0.2954	0.2287	0.024	0.3196	0.0111	0.0332	0.2281	-0.036	0.2637	40.1648	50.3997	30.1227
3	4.374	-4.4658	4.221	4.6152	-3.9376	9.3166	-0.1379	0.0211	0.4475	0.2141	-0.0464	-0.4081	-0.168	0.1676	0.1843	0.3081	-0.055	0.0749	0.2513	-0.0834	0.2805	45.6644	43.6123	42.4822
4	11.27	-7.0319	1.8618	5.5198	-1.9946	6.9357	-0.0464	0.1773	-0.0393	0.3667	-0.3093	-0.5366	-0.2254	-0.3154	0.3556	0.3489	-0.0929	-0.0776	0.2153	0.0252	0.3435	53.9295	32.2263	39.6943
5	8.0364	-14.0642	-4.2464	24.8337	7.5489	2.3198	0.4263	0.2305	-0.5386	-0.9868	-0.5016	0.6815	-0.3226	-0.1121	0.0753	0.4786	0.1185	0.0865	0.4757	-0.2043	0.7015	50.6383	30.6339	29.2061
6	2.4612	1.7386	-3.6661	6.3616	-3.5051	5.6259	-0.3186	-0.3538	-0.3504	0.3199	-0.3436	-1.0617	0.3533	0.5569	0.6622	0.3208	0.046	-0.0521	0.4044	0.0715	0.1797	45.2528	28.7717	44.6571
7	8.1631	0.6457	-0.0506	3.2413	0.5423	0.0996	0.1461	-0.5613	0.1901	0.773	-0.1939	0.287	0.1055	-0.059	0.0477	0.4193	0.0012	0.1136	0.3909	-0.0456	0.0484	37.6946	31.1551	50.0044
8	0.9247	0.8751	0.6273	2.5824	-1.824	3.811	-0.0741	-0.0253	0.1165	-0.1146	-0.0581	0.0852	0.0147	0.137	0.1323	0.2927	0.0437	0.0675	0.264	-0.0643	0.2482	43.4158	40.3409	29.0801
9	3.538	3.7069	-0.2607	7.6248	2.0954	1.8854	0.0344	-0.6501	0.1454	0.0386	-0.3832	0.451	-0.0246	-0.05	0.3488	0.2437	0.0376	0.1271	0.3743	-0.1021	0.2527	28.4364	38.0073	34.3817
10	2.9504	-0.7802	0.7563	1.3305	1.1462	1.8179	-0.4104	-0.1704	0.3114	0.2593	0.1282	0.1154	0.0855	0.0382	0.2822	0.3974	0.0002	0.0698	0.3009	-0.076	0.2329	36.9106	37.0501	37.8864
11	0.6963	1.7649	0.6353	4.5152	1.4318	2.0641	0.0973	-0.0023	-0.0853	0.1644	0.0428	-0.2133	0.1413	-0.0075	-0.14	0.3001	-0.0103	0.075	0.3351	-0.0336	0.263	41.3967	32.1386	37.7147
12	2.2156	-2.1329	-0.8788	15.6972	5.1108	1.7502	0.0119	0.2696	0.0557	0.6204	0.1287	-0.1019	0.1546	-0.0513	-0.1407	0.271	0.006	0.0131	0.277	0.0119	0.2364	36.0419	14.5529	29.8239
13	0.225	-0.2895	0.7124	7.6708	0.0199	2.645	0.1315	-0.1413	0.0645	0.0364	-0.196	0.2666	0.3694	-0.3367	0.0645	0.1263	-0.0642	0.1658	0.3502	-0.0059	0.3481	23.8637	19.5172	28.8345
14	0.9661	1.6424	-0.7374	3.3526	-0.7817	1.0449	0.0527	-0.0819	0.0878	0.2424	-0.2012	0.2443	0.0401	0.0237	0.1485	0.2516	0.0109	0.0504	0.2977	-0.0321	0.3055	29.3486	28.6964	27.7737
15	0.0909	-0.0369	0.1039	2.0329	0.1284	1.4389	0.0369	-0.0007	-0.0207	-0.222	-0.0672	0.3922	0.1922	-0.0975	0.0303	0.2173	-0.0247	-0.0235	0.2891	-0.0053	0.3438	25.4942	16.4976	20.2311
16	1.7142	0.9578	2.1844	5.9516	-3.3412	6.7765	-0.0133	-0.0119	0.0934	-0.1707	0.073	0.4219	0.2931	-0.2062	-0.0597	0.3016	-0.0617	0.0485	0.301	-0.0527	0.352	21.1427	14.9349	15.8039
17	8.9281	-1.9907	-7.1424	4.4794	3.6104	10.6743	-0.0302	0.1368	0.1891	-0.5993	-0.0621	0.5488	0.2489	-0.281	0.0924	0.1657	-0.004	-0.1262	0.263	0.0138	0.354	20.091	24.4319	10.8305
18	0.7201	0.5966	0.533	0.7119	1.5862	9.0028	-0.0808	0.1084	0.0887	-0.0095	-0.0331	0.2198	-0.1623	-0.5261	0.1139	0.1273	-0.042	0.0888	0.2994	-0.0135	0.4082	13.3237	18.9163	24.4932
19	19.4872	-9.9907	0.5114	9.3955	-4.6869	7.5059	0.0275	-0.0453	-0.8366	0.2822	-0.2211	0.6318	-0.6795	-0.2259	0.1936	0.2458	0.0212	0.1501	0.322	-0.0428	0.3322	15.8303	24.2626	35.3808
20	6.5885	2.7222	2.6856	1.1437	1.1061	1.0961	0.153	-0.3008	-0.221	0.1005	-0.0413	-0.0303	0.04	-0.1286	-0.1118	0.3766	-0.0257	0.3248	0.4835	0.1479	0.35	23.6853	15.2677	38.9957
21	12.0528	8.4422	-3.6264	9.6771	1.3735	5.2062	0.7434	-1.5616	0.9712	0.3411	-0.2068	0.2933	-0.3675	1.2548	-0.5366	0.3253	-0.2876	-0.0706	0.6783	-0.3626	0.4762	82.2158	16.1188	54.8281
22	6.3002	4.9241	4.2337	9.5795	2.3178	3.0868	0.2397	0.1976	-0.2147	0.09	-0.1039	-0.3653	0.1061	0.3199	-0.1358	0.2846	-0.0659	-0.0577	0.3472	-0.0248	0.1982	84.7347	13.8962	45.9321
23	2.0702	-0.315	2.664	3.3191	-1.6871	6.9952	0.105	0.1151	0.0802	-0.0502	-0.1139	-0.141	0.4897	0.0379	0.0088	0.2754	0.0097	0.0146	0.227	-0.0024	0.2176	74.3166	4.8674	30.6557
24	4.0095	-2.3936	2.4771	9.3926	8.1336	13.2088	0.3408	-0.3809	0.0513	-0.7041	-0.1893	-0.2057	-0.451	-0.8877	-0.1514	0.1736	0.0566	-0.0472	0.4013	0.0424	0.3116	66.0174	14.5598	28.5502
25	3.0495	2.2061	-1.0356	2.7513	-1.5401	2.7855	0.1067	0.218	-0.3579	0.0988	-0.1407	-0.0887	0.4068	-0.0903	0.034	0.3896	-0.0471	-0.0913	0.194	0.0047	0.2405	67.8454	-3.5433	34.4094
26	5.6513	5.9907	0.0126	8.3565	0.0804	0.8003	0.0419	-0.0142	0.174	-0.1605	0.0429	0.0445	0.0395	-0.0587	-0.0847	0.3172	-0.0225	0.0058	0.2269	0.0169	0.1619	70.3619	7.5194	38.1596
27	4.2401	0.7741	0.1077	5.7495	2.5573	2.8526	0.2052	-0.3389	0.0542	-0.0674	0.0167	-0.0209	-0.0545	0.0074	-0.2219	0.3381	-0.0931	-0.0251	0.2841	0.028	0.1396	74.3737	11.5991	48.3874
28	9.7179	5.6076	1.5483	4.0812	4.7453	18.1099	0.0944	-0.1746	-0.5203	0.0915	0.0447	-0.3082	0.5075	0.5266	-0.1391	0.3634	-0.0404	-0.0162	0.2416	0.0519	0.2084	68.1921	0.9297	53.4294
29	2.5144	0.3514	-3.7322	0.2249	-0.3171	5.7843	0.1327	-0.1961	-0.0527	0.2655	-0.0583	-0.137	0.0961	0.2095	-0.0744	0.4358	-0.0759	-0.0771	0.298	0.0452	0.247	68.2469	3.3986	45.9094
30	5.0759	0.5177	-1.6334	0.114	-0.0647	2.1621	0.1859	0.1223	-0.2039	0.0772	-0.08	-0.0986	0.0132	-0.1588	-0.1059	0.3599	-0.063	-0.0372	0.2552	0.022	0.1888	64.3203	6.7404	38.6428
31	6.1079	-2.2671	-5.8206	13.2882	-9.8264	17.0974	0.0928	0.2308	0.0013	-0.3769	0.134	0.2006	0.2328	-0.4347	-0.2268	0.2672	-0.07	-0.0131	0.3305	-0.0365	0.1863	45.0213	9.8739	41.7336
32	10.6032	3.1012	2.9754	1.0219	0.595	1.8861	0.1918	-0.2239	0.0164	0.0989	-0.1355	0.0988	0.1223	-0.0061	-0.0563	0.3127	-0.055	0.0612	0.3115	-0.0031	0.2045	50.1461	7.6729	56.2838
33	3.0374	-0.2971	-0.7203	1.8566	1.1379	0.8181	-0.011	-0.0309	0.0462	0.108	0.0398	0.0571	0.0399	0.0243	-0.0288	0.4441	-0.123	0.0387	0.2875	0.0301	0.1138	60.3286	10.6268	51.5057
34	10.325	1.1904	-0.8097	0.3679	0.5478	1.883	0.3987	-0.5732	-0.2812	-0.0751	0.0203	-0.1632	-0.3514	0.2698	-0.419	0.3615	-0.1057	0.0562	0.3439	-0.0059	0.2385	64.372	20.4384	61.2556
35	1.6642	-0.9168	-1.3857	0.51	0.7522	1.1896	0.1495	0.0404	0.049	-0.0456	-0.0317	-0.0003	-0.2359	-0.1023	-0.1178	0.4873	-0.0129	0.1648	0.377	0.0956	0.2369	46.0448	16.8182	52.2129
36	13.328	5.6951	3.5079	8.1206	-1.184	2.1902	0.2217	-0.3431	-0.4748	-0.285	-0.0316	-0.0652	0.2255	-0.1411	-0.1901	0.2733	-0.0799	0.1384	0.3499	-0.0239	0.2051	37.6222	12.7177	56.6796
37	2.4366	2.077	0.6118	2.7334	-0.7061	1.7873	-0.0115	-0.3392	0.2137	0.3188	-0.0803	0.1819	-0.2501	-0.4081	0.0918	0.5094	-0.049	0.1353	0.3948	-0.0905	0.1049	49.4808	21.1472	63.1956
38	1.0319	0.212	-1.2344	10.4096	6.1662	5.5327	-0.2796	-0.1158	-0.1142	0.424	0.0501	0.1143	0.5503	0.0577	0.2295	0.4348	-0.0051	0.1127	0.372	0.0037	0.117	46.9463	7.9517	64.8253
39	14.9932	-3.5208	-3.0104	1.4815	3.9599	17.472	-0.0544	-0.4174	-0.5888	0.0636	0.1854	0.0817	0.2619	0.0672	-0.131	0.3505	-0.0473	0.0565	0.3425	0.049	0.2505	54.2835	-0.8977	60.8601
40	5.8466	7.4232	-2.8683	9.6658	-3.6997	1.425	-0.0885	0.1985	-0.381	-0.1645	-0.0392	-0.3737	0.0526	-0.0308	0.1277	0.5512	0.0016	-0	0.3675	-0.1176	0.3376	49.3481	-1.7931	51.1193
41	3.3403	-3.5737	-6.7105	3.9431	7.3637	13.891	-0.3765	0.0719	-0.4333	0.198	-0.2561	0.3797	0.3903	-0.2106	0.6326	0.4234	0.1793	0.1681	0.6467	0.0376	0.4146	61.5455	-2.4297	-5.8437
42	8.4622	0.4233	-1.3228	1.3459	-0.2495	4.0643	-0.0723	0.3719	-0.1042	-0.2109	-0.1578	-0.0577	0.1402	0.153	0.2302	0.2797	-0.0348	0.0087	0.2964	-0.0026	0.2081	59.7033	6.6833	-7.7791
43	9.7036	-4.3663	-0.8596	6.8476	4.0458	4.2241	0.1285	0.8381	0.4127	-0.1311	-0.0789	-0.2123	0.0628	-0.2009	-0.0496	0.2751	-0.0448	-0.0126	0.3	0.0703	0.1449	48.2975	12.9791	-5.2212
44	5.1983	1.2116	7.1416	6.1217	3.998	10.7662	-0.0282	0.1478	0.0917	-0.0405	-0.1478	0.2853	-0.1039	0.0845	0.1759	0.2655	0.0318	0.0505	0.2768	0.0256	0.2873	43.4579	22.4577	7.3807
45	15.9101	9.1821	-1.762	25.0817	12.2232	9.0613	0.4321	0.4173	1.5752	0.5749	0.3838	-0.0806	0.1247	0.0264	-0.8159	0.6501	0.0258	-0.128	0.3306	-0.1075	0.3035	51.3634	19.7068	15.5405
46	3.3733	2.2357	-0.9837	2.0471	1.0001	5.1322	-0.1115	-0.2186	0.263	-0.1069	-0.1308	0.2724	-0.0621	0.0337	0.2423	0.3114	-0.0074	-0.0608	0.2139	-0.0149	0.2858	40.3681	14.521	11.8684
47	0.2779	0.5569	-1.2931	2.0149	-1.7868	7.2937	0.0326	0.0563	0.0178	-0.0938	-0.0251	0.2072	-0.1596	-0.0527	-0.0075	0.2096	0.0132	-0.052	0.3076	-0.0145	0.2779	33.3103	12.1857	1.6591
48	10.1076	2.1151	-4.2275	0.5589	-0.9032	2.1151	-0.0888	0.152	0.1058	-0.0673	0.0531	0.0525	0.0596	-0.0261	0.0357	0.2704	-0.0235	-0.0094	0.2407	-0.0071	0.2646	45.5713	10.9979	6.0328
49	10.0175	1.1573	-4.4438	3.6167	-1.2641	4.5664	-0.0284	-0.0766	0.0023	0.0695	0.1738	0.0257	-0.0929	0.3042	-0.1454	0.2877	-0.0356	-0.0231	0.2588	-0.0052	0.2371	58.8848	8.0806	0.8037
50	3.2837	3.8202	0.8859	5.1443	-0.1893	2.3819	-0.1019	0.0822	-0.0435	-0.1578	0.1702	-0.0234	0.0041	-0.0423	-0.0684	0.281	-0.0472	-0.0029	0.2806	-0.0374	0.2556	48.7503	-1.7245	1.3593
51	0.6415	0.3535	-0.4514	0.9635	-1.0246	1.3638	-0.0052	0.1101	0.0364	-0.0703	0.0268	0.0208	0.0143	-0.138	-0.0215	0.3018	-0.0569	-0.0153	0.2699	-0.0098	0.2603	44.8865	6.977	5.4413
52	0.1863	-0.4143	0.8804	2.2133	-1.042	4.8429	-0.0634	-0.03	-0.0003	0.1379	0.0202	0.1167	-0.2157	-0.191	0.0432	0.299	-0.0133	-0.0049	0.2817	-0.0497	0.2839	43.7269	3.3388	26.6604
53	1.2649	0.6815	-1.745	1.3878	-1.8083	3.7169	0.0385	0.0482	-0.019	0.095	-0.0978	-0.1313	0.1633	-0.1694	0.0593	0.323	-0.0589	-0.0123	0.2355	-0.0063	0.2484	49.4782	-8.7695	17.2073
54	0.7244	0.0114	0.0235	1.2722	-0.8245	0.5609	0.0225	-0.0527	0.078	0.001	-0.0702	0.0022	0.0436	0.0202	0.0477	0.3293	-0.0636	-0.0254	0.2326	-0.0313	0.2649	49.0798	-3.1639	14.2292
55	0.1871	-0.5691	-1.07	2.4624	2.9237	6.712	0.0275	-0.0181	-0.0001	0.1407	-0.0847	-0.2067	0.0318	0.0543	0.0572	0.3139	-0.028	-0.0366	0.2501	0.0081	0.2745	55.4257	-7.6722	25.4067
56	10.8718	-1.152	-7.0065	2.9753	0.2126	4.6166	0.1447	-0.0875	-0.1465	0.6381	-0.2564	-0.1432	-0.1949	0.0911	0.1117	0.4184	-0.12	-0.0416	0.2944	0.0243	0.1319	45.7876	-11.9599	34.6319
57	7.8931	-0.7639	-3.3128	1.5543	1.5716	2.4594	0.0854	-0.1278	-0.3207	0.1985	-0.1176	-0.1377	0.1213	-0.0227	0.0322	0.3015	0.0024	-0.0316	0.2222	0.0467	0.291	60.7588	-15.3989	26.2373
58	6.8197	1.1132	-0.1599	0.4722	-0.6368	1.8483	0.0981	0.0296	-0.0983	-0.0485	0.025	-0.0875	0.5077	0.0445	-0.1231	0.4408	0.0099	-0.0552	0.1589	-0.0097	0.2634	53.3005	-19.5591	21.0774
59	1.2447	-0.5076	0.4461	8.638	-4.2856	4.2127	0.0351	0.0132	-0.1299	-0.1852	0.0077	0.1207	0.4574	-0.4138	-0.0427	0.286	-0.0911	-0.0132	0.235	-0.0012	0.2336	38.8046	-15.7416	17.6408
60	10.4138	0.7305	4.7275	2.7455	-2.6887	5.5571	-0.4296	-1.1738	-0.1792	0.1877	0.2637	-0.1955	-0.5477	-0.9524	0.1659	0.5237	0.157	-0.1468	0.4417	-0.0905	0.2555	35.3924	-5.5277	26.1992
61	33.3856	1.7463	-22.4394	0.1052	-1.179	15.0894	-0.3849	0.8406	-0.3295	-0.0077	0.1235	0.0158	0.2796	-0.5795	0.2614	0.4051	0.1315	0.0522	0.4955	0.319	0.7417	30.6018	42.9026	11.1024
62	4.3456	-1.6642	-2.6595	4.3891	0.6554	5.8205	-0.0144	0.0502	0.0586	0.1719	0.0974	0.3501	0.1051	0.0158	-0.083	0.2224	0.0094	-0.0063	0.2442	0.0339	0.2905	36.1638	42.2262	6.9874
63	6.7231	-2.2202	-10.5836	3.8157	3.8021	16.8019	0.1569	0.7779	0.0254	0.0934	-0.1931	0.3182	-0.1981	-1.0443	0.0361	0.2555	0.0365	-0.0599	0.3832	0.0447	0.2808	38.4395	38.8268	-0.4204
64	7.4719	0.2498	3.8661	7.6347	-4.2596	4.5266	0.0369	0.4071	-0.2704	-0.0698	0.113	0.0114	0.0664	0.1433	-0.1499	0.3335	0.0298	0.0196	0.2833	-0.0681	0.2146	50.9246	29.1348	-2.8999
65	10.9652	-8.7067	-2.2695	7.4379	0.572	3.7478	0.0553	-0.1637	0.2943	-0.0526	0.2457	-0.3043	0.1265	-0.2012	-0.3011	0.2681	-0.0029	-0.0417	0.2058	-0.0197	0.4709	55.997	27.1663	5.8858
66	4.2635	1.5358	-2.0967	0.5768	-0.751	1.0328	-0.2253	-0.0756	-0.3222	-0.0656	0.0554	-0.0884	0.1368	0.0479	0.1699	0.3954	0.0228	-0.028	0.2711	0.0311	0.2227	59.9532	34.0248	-2.5215
67	4.561	1.8116	-2.699	4.2082	-3.9714	4.2978	-0.4817	0.3616	0.3139	-0.5993	0.2979	-0.0384	0.4035	-0.3261	0.1838	0.4189	-0.0067	-0.1	0.3079	0.0395	0.2599	55.3135	39.1791	-10.7705
68	5.8882	2.4987	-4.4331	1.1321	-1.7314	3.9533	0.0287	0.1869	0.0155	0.0445	-0.0234	-0.0294	-0.0113	-0.2352	-0.0053	0.314	0.0245	-0.0162	0.232	0.0522	0.2593	51.9839	37.456	3.3348
69	0.7621	1.5677	-0.7797	5.6014	-2.6091	2.1134	0.0577	-0.0076	0.1272	0.1644	-0.0757	0.1599	-0.1184	-0.0691	0.0181	0.3091	0.0396	-0.0333	0.2082	0.0295	0.277	45.6648	47.9536	5.4841
70	0.8593	0.3242	0.1703	2.1652	0.2483	0.0722	-0.0858	-0.0344	0.143	-0.1647	-0.0126	0.0767	-0.0185	-0.0295	0.0984	0.3027	0.0067	-0.0495	0.2705	0.0403	0.2869	51.3969	42.3413	1.4104
71	4.3516	0.6618	4.8536	0.9703	-0.1141	6.2554	0.0048	0.197	-0.2846	-0.0498	0.1895	-0.206	0.0913	0.0669	-0.1944	0.2794	0.0699	-0.0742	0.2389	-0.0347	0.3352	69.2383	45.0207	8.6692
72	11.3987	0.8138	0.0065	0.1319	0.2016	0.5604	-0.159	-0.091	0.0628	-0.0327	0.0315	0.0703	-0.0797	0.1171	0.1276	0.2926	-0.005	-0.0346	0.1883	0.0269	0.329	68.7506	57.4554	9.5755
73	4.775	1.6802	-0.7845	2.0958	0.3936	0.4371	0.2879	-0.4254	0.6128	-0.0177	-0.1612	0.0583	-0.0806	0.0282	-0.1267	0.303	0.0016	0.0174	0.2273	-0.07	0.3586	52.5755	56.3952	12.4692
74	0.3652	0.3338	0.4684	0.4256	0.0799	1.8004	0.0247	0.0134	0.0993	0.0794	-0.0375	0.0622	-0.1248	0.0307	0.0128	0.3238	0.0221	-0.0462	0.2149	0.0316	0.3322	58.6538	51.5425	8.3569
75	3.4389	0.3068	1.4838	0.3816	-0.5187	1.8582	0.0131	-0.2672	0.154	0.0648	-0.1312	0.0245	-0.1624	0.0544	0.1181	0.2842	0.0022	-0.0488	0.2152	-0.0892	0.3592	63.5318	57.0164	20.1042
76	12.0659	0.1852	-10.5146	0.0053	-0.1618	9.1707	-0.1559	0.1966	-0.1752	0.0054	-0.027	-0.0035	0.0897	-0.1693	0.1828	0.2942	-0.0826	-0.0773	0.2605	-0.0225	0.3352	77.9968	61.2853	15.0444
77	6.1775	0.0473	-0.9302	2.3729	-1.2774	6.936	0.0469	-0.8163	0.7866	0.0905	-0.085	-0.0429	-0.5872	-0.0886	0.0382	0.2566	0.0282	-0.0365	0.1505	-0.1769	0.3668	62.1692	65.6926	21.9513
78	8.9682	4.1341	-0.6301	3.5011	1.3106	1.6539	-0.1979	-0.0005	1.4162	-0.6808	0.269	0.6543	-0.5896	0.2759	-0.0711	0.4088	-0.1005	-0.0435	0.0481	0.0228	0.6201	68.4771	69.3733	10.081
79	7.3815	-8.7643	-0.2069	10.9079	-0.561	6.2523	-0.2655	0.347	0.0883	0.1949	0.1204	-0.0065	-0.5382	0.1312	0.145	0.3227	-0.2434	-0.0275	0.7785	0.1932	0.3441	65.1884	62.1643	-0.6448
80	11.4628	-2.3144	3.1852	0.8439	1.4114	12.1093	-0.2534	0.4059	0.6787	-0.023	0.0959	-0.1333	-0.5089	1.1006	0.1574	0.2651	-0.0366	0.0109	0.3134	0.0555	0.3246	75.5781	54.7495	2.7521
81	5.824	-4.7722	-2.1933	4.3226	0.6513	4.7021	0.2889	0.2724	-0.3364	-0.3474	-0.2167	0.2589	0.8208	-0.0524	-0.0722	0.5682	-0.0022	-0.2185	0.1557	-0.0346	0.2672	75.1023	-2.048	2.3407
82	1.7905	-0.3554	-1.4044	7.3203	6.3895	9.1232	0.0996	-0.2195	-0.1447	-0.1323	0.1069	-0.1259	0.0054	-0.1461	-0.2065	0.2647	-0.0031	-0.0195	0.2283	0.0093	0.2696	84.5417	4.0957	16.2898
83	2.8343	-2.3226	5.6384	7.2899	-2.8491	11.8104	0.109	0.1984	0.0163	0.1412	-0.1663	-0.0683	0.3291	0.406	0.0573	0.2853	-0.0082	0.0323	0.2764	0.0098	0.2525	82.8841	12.8875	23.1528
84	7.6797	-0.6224	1.6433	2.9008	4.5516	8.4952	-0.1135	-0.3269	-0.1154	0.026	0.1738	0.1318	-0.1162	0.2344	-0.0603	0.2566	-0.011	0.03	0.2485	-0.0029	0.2898	76.6692	16.7423	25.5153
85	7.3592	3.552	-0.0852	9.8446	4.4169	2.4981	0.2735	0.2791	0.8056	-0.1864	-0.3158	0.2116	-0.1671	-0.3848	0.0423	0.2975	0.0483	0.0599	0.5008	-0.2333	0.5423	68.7007	18.7448	17.4905
86	7.8087	6.4473	2.6157	5.8975	2.1689	0.9039	-0.0411	0.0909	0.1946	0.01	0.073	0.1722	-0.0113	0.0634	-0.0319	0.2317	-0.0034	0.0033	0.2515	-0.0434	0.3724	78.3558	19.6858	15.6053
87	0.679	-2.427	0.0515	11.4635	-0.9622	0.2527	0.1056	-0.1261	0.1162	-0.2104	-0.147	-0.3204	-0.0196	0.1003	0.0414	0.2495	-0.0514	0.0049	0.3246	-0.0531	0.3159	90.9515	18.4353	21.6016
88	5.3377	2.454	0.7668	2.4816	0.1457	1.2811	0.1168	-0.2547	0.1665	0.0222	-0.0464	0.0031	0.0216	-0.0273	-0.0704	0.2282	-0.0135	0.0078	0.2091	-0.0029	0.2364	84.7053	13.3937	14.1427
89	2.8634	0.0209	-1.6984	1.8433	-2.1701	3.5751	0.1493	-0.0797	0.1387	-0.1916	0.062	0.021	0.183	0.0264	-0.2113	0.2665	0.0367	-0.0144	0.2245	-0.0764	0.3516	81.1202	8.9523	2.1114
90	0.5307	0.4189	-1.0804	0.9166	-1.6054	4.6935	-0.0292	0.0983	0.0331	0.0458	0.0744	-0.2906	0.2262	0.109	-0.0452	0.2343	0.0245	-0.0164	0.2676	-0.0342	0.3223	85.2602	13.4924	6.6579
91	1.1573	0.1735	0.0012	3.6206	-2.2516	1.5479	-0.1213	-0.0708	0.0167	0.0542	-0.0034	-0.2935	-0.0571	0.0445	0.1247	0.284	-0.0179	-0.0183	0.2089	-0.047	0.2871	81.1899	21.6466	10.2987
92	7.2135	-2.4269	-5.708	1.4979	1.0131	11.2643	-0.0316	-0.05	0.5659	0.2122	0.2137	-0.2689	-0.6468	-0.0824	-0.1821	0.297	-0.0366	-0.0181	0.1164	-0.0309	0.3277	81.3206	39.1149	2.2161
93	2.1778	0.2761	0.9051	3.2688	-0.6665	0.8097	0.0664	0.2132	0.0535	-0.0329	-0.0867	-0.2244	0.0201	0.0494	0.0203	0.2352	-0.0014	0.0601	0.2738	-0.0067	0.2656	80.7543	22.8753	-3.9399
94	10.8779	-2.5419	4.7375	3.3248	0.7215	3.4722	-0.1685	0.2505	-0.43	-0.0235	0.1884	0.2407	0.0827	0.0635	-0.0199	0.3794	-0.1172	0.0435	0.3739	-0.0523	0.2484	69.454	12.0909	-10.9008
95	0.8298	0.4909	0.5468	6.6409	2.5836	1.1826	0.0992	0.2028	0.1999	-0.3523	-0.116	-0.3356	-0.088	0.0003	0.0168	0.3307	-0.0194	0.0256	0.2957	0.0178	0.2563	73.1139	30.8465	-7.8071
96	7.1456	3.5071	0.9159	7.1242	4.2582	2.8889	-0.0487	0.249	0.4343	-0.0706	0.0026	0.0303	-0.1271	-0.0506	0.0461	0.3249	-0.0247	-0.0073	0.2203	0.0943	0.2874	78.6532	41.1401	-12.0014
97	0.6091	0.1553	-0.4733	1.4086	-0.3562	4.8154	-0.0912	0.5134	0.2205	-0.5091	-0.0352	0.0143	0.03	0.0095	0.1264	0.4214	-0.0723	0.0068	0.5477	0.1415	0.1919	69.3944	27.7418	-16.9347
98	3.4701	-1.4207	-1.1854	12.7657	-0.4744	4.1001	0.1684	0.4214	0.0096	-0.1398	-0.4299	-0.3928	0.2247	0.0443	0.2615	0.2649	-0.0048	0.0817	0.362	0.0574	0.1541	80.9996	28.2411	-19.6404
99	11.2978	-7.6824	4.4692	12.4259	4.2471	9.2304	-0.4104	0.3685	0.1492	-0.3604	-0.0499	0.1618	-0.6819	0.3207	0.4603	0.2494	-0.0765	0.156	0.2909	0.1114	0.2854	86.9028	33.9838	-15.7884
100	4.7115	-0.4795	2.7594	4.0245	1.6113	6.3869	-0.0082	0.6222	0.6192	0.0223	-0.086	-0.9233	-0.0987	0.6539	0.0943	0.1547	-0.0797	0.0885	0.2333	0.1404	0.4176	91.8924	30.1355	-5.8934
101	2.1455	2.2673	3.4243	5.0578	-1.0029	13.5269	-0.171	0.2572	0.1196	-0.342	0.057	0.2222	0.0108	0.8742	0.114	0.2761	0.0007	0.0824	0.4128	0.1665	0.2723	81.2944	32.164	56.6616
102	0.4265	-0.8893	-0.6735	11.316	-6.5963	9.5805	-0.1515	-0.166	-0.0373	0.2573	0.0903	-0.1944	-0.0878	-0.0839	0.0613	0.3496	0.0218	0.0705	0.3772	0.0582	0.2055	71.0011	32.8663	54.7566
103	1.7584	-0.7359	1.3196	13.5736	-1.6954	1.0924	-0.4258	-0.4399	0.1644	0.9453	0.2851	-0.1117	-0.3723	-0.3651	0.1408	0.2863	0.0201	0.0584	0.3365	-0.0815	0.2039	65.8535	43.0708	49.8266
104	5.9541	2.4978	-1.4651	3.5934	4.0068	8.7995	0.3094	0.0297	0.3881	-0.3355	-0.1597	0.1293	-0.8667	-0.4414	-0.1497	0.3233	0.0662	-0.0307	0.3469	-0.0762	0.2913	58.4387	47.7767	38.2987
105	3.3202	0.7042	4.551	2.6354	-1.1277	8.2903	-0.1725	0.0916	-0.009	-0.2749	0.2123	-0.1248	0.2105	-0.3605	-0.0399	0.3483	-0.1845	-0.0171	0.4818	0.0435	0.2055	61.2725	38.5698	32.4593
106	0.9348	0.4291	-0.3433	1.6506	-0.9723	5.2209	-0.102	0.0362	0.0832	0.0393	-0.0531	0.3948	-0.549	-0.0341	0.155	0.2737	-0.0205	-0.0539	0.1926	-0.0937	0.3166	68.8012	53.372	34.536
107	1.3992	0.881	-1.4535	8.9509	-5.8048	4.4839	-0.2085	0.1435	-0.1628	0.1734	-0.0775	-0.09	0.0357	-0.2067	0.2861	0.3035	-0.0922	0.0499	0.2572	-0.1158	0.2671	68.7349	34.49	42.3514
108	1.264	2.1976	-1.4128	7.7163	-1.1275	2.2046	0.0028	-0.1564	-0.2328	-0.1989	-0.3959	-0.1873	-0.0357	-0.0884	0.3931	0.2998	-0.0797	0.0048	0.3165	-0.0261	0.2448	76.6275	37.4259	46.4729
109	4.3071	0.8055	-1.9086	0.2036	-0.1924	1.4192	-0.0043	-0.0532	0.1959	0.0142	-0.0345	0.0621	0.1334	-0.1691	0.0388	0.3233	-0.091	-0.0024	0.3004	-0.0286	0.2959	84.7092	46.0255	44.6175
110	3.4488	0.4123	-1.4504	0.9004	0.4512	1.0873	-0.0507	0.0825	-0.069	0.0864	-0.0405	-0.018	0.0584	-0.1062	0.0913	0.3424	-0.0573	-0.0264	0.223	-0.0789	0.2969	74.9418	43.4043	36.5844
111	4.1806	-0.125	3.1776	0.7088	0.2249	2.911	-0.1792	-0.1192	-0.3746	-0.3528	0.4301	-0.4097	-0.4164	-0.1581	-0.2509	0.3505	-0.2355	0.085	0.5102	-0.3069	0.4636	90.0418	41.461	21.8587
112	4.1228	-1.8345	1.7443	1.928	-0.2777	8.1956	-0.0989	-0.0093	-0.2765	0.072	-0.1973	-0.2402	-0.1663	0.0176	0.2962	0.2464	-0.0477	-0.0202	0.2214	0.0035	0.3207	92.9888	38.6708	40.6278
113	2.4606	0.4656	-0.9498	0.9788	-1.2133	1.5916	-0.374	0.1333	-0.3546	0.1586	0.153	-0.2047	-0.1794	-0.1785	0.221	0.3421	-0.1109	0.0859	0.136	-0.0688	0.3862	84.9471	38.276	33.5377
114	1.3333	-1.7913	3.6194	6.0693	-5.0835	10.0561	0.1839	0.0236	-0.1419	-0.0112	-0.0531	-0.1737	0.3604	0.2485	-0.1309	0.2683	-0.0672	-0.0417	0.2257	0.1298	0.5577	91.4824	28.7197	43.7885
115	0.6383	-0.0433	2.1809	2.0267	-1.6633	9.0838	-0.1308	0.0935	-0.0828	0.0049	-0.0126	-0.3316	-0.1184	0.1254	0.1433	0.2802	-0.0948	0.0733	0.1927	-0.0865	0.3989	93.3495	29.9127	27.4546
116	10.3412	-2.8454	2.5301	0.8574	-0.6836	0.6223	0.6941	1.1836	-0.4218	-0.0858	-0.5542	0.0835	0.1849	0.2589	-0.1399	0.5165	-0.3521	0.1928	0.7735	-0.0727	0.2908	95.7763	38.2522	14.8029
117	4.8215	2.0867	-7.695	10.006	-9.311	16.2108	-0.1424	0.0869	-0.097	-0.7698	-0.4185	-0.6562	0.7046	0.1536	0.5609	0.2619	-0.1152	0.1244	0.2296	-0.1479	0.3341	101.1346	33.9572	19.8854
118	3.0307	-3.8001	2.5262	8.747	-0.791	3.5237	0.2391	0.4477	-0.1782	0.1417	-0.2148	0.4498	0.4833	0.5867	-0.0243	0.1695	0.0321	0.2725	0.1437	-0.142	0.7386	99.8122	24.4263	28.7186
119	2.1801	-0.6443	-1.4653	6.5652	-0.4473	1.452	-0.1017	-0.0481	-0.3183	0.5293	-0.0094	-0.0088	-0.1679	0.4176	0.1111	0.1961	-0.1602	0.0701	0.4353	-0.0835	0.4406	103.2886	34.2925	32.1431
120	3.1597	-1.5131	-0.308	4.7442	-3.7943	3.9926	-0.0187	0.1359	-0.1863	0.4886	0.0095	-0.0524	-0.6384	0.0388	0.0092	0.4605	-0.2521	0.086	0.1999	-0.1631	0.37	95.9808	49.364	28.5683

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	A	68 - 73
2	X-RAY DIFFRACTION	2	A	74 - 80
3	X-RAY DIFFRACTION	3	A	81 - 89
4	X-RAY DIFFRACTION	4	A	90 - 99
5	X-RAY DIFFRACTION	5	A	100 - 107
6	X-RAY DIFFRACTION	6	A	108 - 118
7	X-RAY DIFFRACTION	7	A	119 - 133
8	X-RAY DIFFRACTION	8	A	134 - 152
9	X-RAY DIFFRACTION	9	A	153 - 166
10	X-RAY DIFFRACTION	10	A	167 - 177
11	X-RAY DIFFRACTION	11	A	178 - 189
12	X-RAY DIFFRACTION	12	A	190 - 198
13	X-RAY DIFFRACTION	13	A	199 - 219
14	X-RAY DIFFRACTION	14	A	220 - 252
15	X-RAY DIFFRACTION	15	A	285 - 326
16	X-RAY DIFFRACTION	16	A	327 - 335
17	X-RAY DIFFRACTION	17	A	336 - 345
18	X-RAY DIFFRACTION	18	A	346 - 359
19	X-RAY DIFFRACTION	19	A	360 - 365
20	X-RAY DIFFRACTION	20	A	366 - 372
21	X-RAY DIFFRACTION	21	B	65 - 72
22	X-RAY DIFFRACTION	22	B	73 - 80
23	X-RAY DIFFRACTION	23	B	81 - 97
24	X-RAY DIFFRACTION	24	B	98 - 107
25	X-RAY DIFFRACTION	25	B	108 - 127
26	X-RAY DIFFRACTION	26	B	128 - 146
27	X-RAY DIFFRACTION	27	B	147 - 159
28	X-RAY DIFFRACTION	28	B	160 - 164
29	X-RAY DIFFRACTION	29	B	165 - 176
30	X-RAY DIFFRACTION	30	B	177 - 193
31	X-RAY DIFFRACTION	31	B	194 - 202
32	X-RAY DIFFRACTION	32	B	203 - 216
33	X-RAY DIFFRACTION	33	B	217 - 251
34	X-RAY DIFFRACTION	34	B	252 - 291
35	X-RAY DIFFRACTION	35	B	292 - 320
36	X-RAY DIFFRACTION	36	B	321 - 328
37	X-RAY DIFFRACTION	37	B	329 - 346
38	X-RAY DIFFRACTION	38	B	347 - 359
39	X-RAY DIFFRACTION	39	B	360 - 364
40	X-RAY DIFFRACTION	40	B	365 - 371
41	X-RAY DIFFRACTION	41	C	66 - 71
42	X-RAY DIFFRACTION	42	C	72 - 78
43	X-RAY DIFFRACTION	43	C	79 - 87
44	X-RAY DIFFRACTION	44	C	88 - 98
45	X-RAY DIFFRACTION	45	C	99 - 103
46	X-RAY DIFFRACTION	46	C	107 - 116
47	X-RAY DIFFRACTION	47	C	117 - 126
48	X-RAY DIFFRACTION	48	C	127 - 145
49	X-RAY DIFFRACTION	49	C	146 - 150
50	X-RAY DIFFRACTION	50	C	151 - 164
51	X-RAY DIFFRACTION	51	C	165 - 187
52	X-RAY DIFFRACTION	52	C	188 - 199
53	X-RAY DIFFRACTION	53	C	200 - 229
54	X-RAY DIFFRACTION	54	C	230 - 288
55	X-RAY DIFFRACTION	55	C	289 - 306
56	X-RAY DIFFRACTION	56	C	307 - 328
57	X-RAY DIFFRACTION	57	C	329 - 340
58	X-RAY DIFFRACTION	58	C	341 - 354
59	X-RAY DIFFRACTION	59	C	355 - 368
60	X-RAY DIFFRACTION	60	C	369 - 372
61	X-RAY DIFFRACTION	61	D	71 - 73
62	X-RAY DIFFRACTION	62	D	74 - 79
63	X-RAY DIFFRACTION	63	D	80 - 84
64	X-RAY DIFFRACTION	64	D	85 - 93
65	X-RAY DIFFRACTION	65	D	94 - 107
66	X-RAY DIFFRACTION	66	D	108 - 117
67	X-RAY DIFFRACTION	67	D	118 - 128
68	X-RAY DIFFRACTION	68	D	129 - 144
69	X-RAY DIFFRACTION	69	D	145 - 164
70	X-RAY DIFFRACTION	70	D	165 - 185
71	X-RAY DIFFRACTION	71	D	186 - 197
72	X-RAY DIFFRACTION	72	D	198 - 216
73	X-RAY DIFFRACTION	73	D	217 - 229
74	X-RAY DIFFRACTION	74	D	230 - 253
75	X-RAY DIFFRACTION	75	D	286 - 307
76	X-RAY DIFFRACTION	76	D	317 - 327
77	X-RAY DIFFRACTION	77	D	328 - 349
78	X-RAY DIFFRACTION	78	D	350 - 359
79	X-RAY DIFFRACTION	79	D	360 - 368
80	X-RAY DIFFRACTION	80	D	369 - 372
81	X-RAY DIFFRACTION	81	E	64 - 80
82	X-RAY DIFFRACTION	82	E	81 - 87
83	X-RAY DIFFRACTION	83	E	88 - 92
84	X-RAY DIFFRACTION	84	E	93 - 98
85	X-RAY DIFFRACTION	85	E	99 - 105
86	X-RAY DIFFRACTION	86	E	106 - 112
87	X-RAY DIFFRACTION	87	E	113 - 119
88	X-RAY DIFFRACTION	88	E	120 - 146
89	X-RAY DIFFRACTION	89	E	147 - 164
90	X-RAY DIFFRACTION	90	E	165 - 177
91	X-RAY DIFFRACTION	91	E	178 - 195
92	X-RAY DIFFRACTION	92	E	196 - 204
93	X-RAY DIFFRACTION	93	E	205 - 250
94	X-RAY DIFFRACTION	94	E	251 - 286
95	X-RAY DIFFRACTION	95	E	287 - 318
96	X-RAY DIFFRACTION	96	E	319 - 330
97	X-RAY DIFFRACTION	97	E	331 - 345
98	X-RAY DIFFRACTION	98	E	346 - 352
99	X-RAY DIFFRACTION	99	E	353 - 358
100	X-RAY DIFFRACTION	100	E	359 - 372
101	X-RAY DIFFRACTION	101	F	65 - 71
102	X-RAY DIFFRACTION	102	F	72 - 79
103	X-RAY DIFFRACTION	103	F	80 - 86
104	X-RAY DIFFRACTION	104	F	87 - 91
105	X-RAY DIFFRACTION	105	F	92 - 107
106	X-RAY DIFFRACTION	106	F	108 - 132
107	X-RAY DIFFRACTION	107	F	133 - 147
108	X-RAY DIFFRACTION	108	F	148 - 158
109	X-RAY DIFFRACTION	109	F	159 - 164
110	X-RAY DIFFRACTION	110	F	165 - 193
111	X-RAY DIFFRACTION	111	F	194 - 213
112	X-RAY DIFFRACTION	112	F	214 - 222
113	X-RAY DIFFRACTION	113	F	223 - 249
114	X-RAY DIFFRACTION	114	F	250 - 288
115	X-RAY DIFFRACTION	115	F	289 - 304
116	X-RAY DIFFRACTION	116	F	305 - 323
117	X-RAY DIFFRACTION	117	F	324 - 330
118	X-RAY DIFFRACTION	118	F	331 - 339
119	X-RAY DIFFRACTION	119	F	340 - 354
120	X-RAY DIFFRACTION	120	F	355 - 372